LOCUS       sequence-270199-        9611 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway destination clone of GFP (as control) tagged with C-terminal
            V5 for generating protein-proximity networks using 
            proximity-dependent biotinylation proteomics .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9611)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 9611)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p667-UBC-GFP-V5_IDG-K
FEATURES             Location/Qualifiers
     source          1..9611
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    27..48
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(63..68,69..86,87..93)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    101..117
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     106..128
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     125..141
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     125..141
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     160..180
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        162..180
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        208..434
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             435..615
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    662..787
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1280..1513
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1698..1742
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2009..2125
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    2157..2177
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        2177..3388
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     3225..3244
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    3389..3413
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(3434..3436,3437..3439,3440..4150)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3479..3500)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3740..3759)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4087..4108
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(4170..4194)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4193..4234
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(4293..4312)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     protein_bind    complement(4429..4449)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     misc_feature    4478..5066
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4531..4551)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4949..4960)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        5080..5579
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5115..5138)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5512..5531
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             5600..6199
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5600..5619)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6096..6116
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             6337..6570
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6642..6763
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6679..6698)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6733..6752
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6803..6938
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6958..6977)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6959..6977)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6987..7004)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6987..7003)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6996..7018)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7145..7600
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7232..7251)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7442..7463
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7626..7730
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7731..7799,7800..8591)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7949..7968)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8762..9350
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9251..9270
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9504..9521
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag
       61 gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt
      121 cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc tcactaaagg
      181 gaacaaaagc tggagctgca agcttaatgt agtcttatgc aatactcttg tagtcttgca
      241 acatggtaac gatgagttag caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc
      301 gattggtgga agtaaggtgg tacgatcgtg ccttattagg aaggcaacag acgggtctga
      361 catggattgg acgaaccact gaattgccgc attgcagaga tattgtattt aagtgcctag
      421 ctcgatacat aaacgggtct ctctggttag accagatctg agcctgggag ctctctggct
      481 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
      541 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
      601 ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga
      661 gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg
      721 actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga
      781 gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc
      841 agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg
      901 attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca
      961 gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc
     1021 aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa
     1081 gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg ctgatcttca
     1141 gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa tataaagtag
     1201 taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag
     1261 aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca
     1321 ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag
     1381 tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca
     1441 cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg
     1501 atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc
     1561 cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga
     1621 tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat
     1681 cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt
     1741 tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag
     1801 taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta
     1861 ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacccgaca
     1921 ggcccgaagg aatagaagaa gaaggtggag agagagacag agacagatcc attcgattag
     1981 tgaacggatc tcgacggtat cggttaactt ttaaaagaaa aggggggatt ggggggtaca
     2041 gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa
     2101 aacaaattac aaaattcaaa attttatcgt ccagtgtggt ggaattctgc agatatcaac
     2161 tttgtataga aaagttggcc tccgcgccgg gttttggcgc ctcccgcggg cgcccccctc
     2221 ctcacggcga gcgctgccac gtcagacgaa gggcgcagcg agcgtcctga tccttccgcc
     2281 cggacgctca ggacagcggc ccgctgctca taagactcgg ccttagaacc ccagtatcag
     2341 cagaaggaca ttttaggacg ggacttgggt gactctaggg cactggtttt ctttccagag
     2401 agcggaacag gcgaggaaaa gtagtccctt ctcggcgatt ctgcggaggg atctccgtgg
     2461 ggcggtgaac gccgatgatt atataaggac gcgccgggtg tggcacagct agttccgtcg
     2521 cagccgggat ttgggtcgcg gttcttgttt gtggatcgct gtgatcgtca cttggtgagt
     2581 agcgggctgc tgggctggcc ggggctttcg tggccgccgg gccgctcggt gggacggaag
     2641 cgtgtggaga gaccgccaag ggctgtagtc tgggtccgcg agcaaggttg ccctgaactg
     2701 ggggttgggg ggagcgcagc aaaatggcgg ctgttcccga gtcttgaatg gaagacgctt
     2761 gtgaggcggg ctgtgaggtc gttgaaacaa ggtggggggc atggtgggcg gcaagaaccc
     2821 aaggtcttga ggccttcgct aatgcgggaa agctcttatt cgggtgagat gggctggggc
     2881 accatctggg gaccctgacg tgaagtttgt cactgactgg agaactcggt ttgtcgtctg
     2941 ttgcgggggc ggcagttatg gcggtgccgt tgggcagtgc acccgtacct ttgggagcgc
     3001 gcgccctcgt cgtgtcgtga cgtcacccgt tctgttggct tataatgcag ggtggggcca
     3061 cctgccggta ggtgtgcggt aggcttttct ccgtcgcagg acgcagggtt cgggcctagg
     3121 gtaggctctc ctgaatcgac aggcgccgga cctctggtga ggggagggat aagtgaggcg
     3181 tcagtttctt tggtcggttt tatgtaccta tcttcttaag tagctgaagc tccggttttg
     3241 aactatgcgc tcggggttgg cgagtgtgtt ttgtgaagtt ttttaggcac cttttgaaat
     3301 gtaatcattt gggtcaatat gtaattttca gtgttagact agtaaattgt ccgctaaatt
     3361 ctggccgttt ttggcttttt tgttagacgc aactttgtac aaaaaagcag gctccgcggc
     3421 cgcccccttc accatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct
     3481 ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtctggcg agggcgaggg
     3541 cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt
     3601 gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc
     3661 cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga
     3721 gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga
     3781 gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa
     3841 catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga
     3901 caagcagaag aacggcatca aggcgaactt caagatccgc cacaacatcg aggacggcag
     3961 cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct
     4021 gcccgacaac cactacctga gcacccagtc cgccctgagc aaagacccca acgagaagcg
     4081 cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga
     4141 gctgtacaag tccaagggtg ggcgcgccga cccagctttc ttgtacaaag tgggcaagcc
     4201 tatccctaac cctctcctcg gtctcgattc tacgtaataa cactggatat accaccgttg
     4261 atatatccca atggcatcgt aaagaacatt ttgaggcatt tcagtcagtt gctcaatgta
     4321 cctataacca gaccgttcag ctggatatta cggccttttt aaagaccgta aagaaaaata
     4381 agcacaagtt ttatccggcc tttattcaca ttcttgcccg cctgtaatca actttattat
     4441 acatagttga tatccagcac agtggcggcc gctcgacaat caacctctgg attacaaaat
     4501 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc
     4561 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt
     4621 gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg
     4681 cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg
     4741 tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc
     4801 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt
     4861 gttgtcgggg aagctgacgt cctttccatg gctgctcgcc tgtgttgcca cctggattct
     4921 gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg
     4981 cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg
     5041 gatctccctt tgggccgcct ccccgcctgg aattctaccg ggtaggggag gcgcttttcc
     5101 caaggcagtc tggagcatgc gctttagcag ccccgctggg cacttggcgc tacacaagtg
     5161 gcctctggcc tcgcacacat tccacatcca ccggtaggcg ccaaccggct ccgttctttg
     5221 gtggcccctt cgcgccacct tctactcctc ccctagtcag gaagttcccc cccgccccgc
     5281 agctcgcgtc gtgcaggacg tgacaaatgg aagtagcacg tctcactagt ctcgtgcaga
     5341 tggacagcac cgctgagcaa tggaagcggg taggcctttg gggcagcggc caatagcagc
     5401 tttgctcctt cgctttctgg gctcagaggc tgggaagggg tgggtccggg ggcgggctca
     5461 ggggcgggct caggggcggg gcgggcgccc gaaggtcctc cggaggcccg gcattctgca
     5521 cgcttcaaaa gcgcacgtct gccgcgctgt tctcctcttc ctcatctccg ggcctttcga
     5581 cctgcagccc aagcttacca tgaccgagta caagcccacg gtgcgcctcg ccacccgcga
     5641 cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg
     5701 ccacaccgtc gatccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct
     5761 cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc
     5821 ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg
     5881 catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc
     5941 gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca
     6001 ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc
     6061 cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct
     6121 cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac
     6181 ccgcaagccc ggtgcctgac gcccgcccca cgacccgcag cgcccgaccg aaaggagcgc
     6241 acgaccccat gcatctcgag ggcccggtac ctttaagacc aatgacttac aaggcagctg
     6301 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctagct cactcccaac
     6361 gaagacaaga tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct
     6421 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     6481 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     6541 ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt
     6601 atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt
     6661 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     6721 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc
     6781 tatcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     6841 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
     6901 gctattccag aagtagtgag gaggcttttt tggaggccta gggacgtacc caattcgccc
     6961 tatagtgagt cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa
     7021 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
     7081 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
     7141 tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     7201 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     7261 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     7321 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
     7381 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     7441 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
     7501 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     7561 tttaacgcga attttaacaa aatattaacg cttacaattt aggtggcact tttcggggaa
     7621 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     7681 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     7741 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     7801 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     7861 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     7921 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
     7981 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     8041 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     8101 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     8161 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     8221 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     8281 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     8341 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     8401 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     8461 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     8521 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     8581 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     8641 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     8701 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     8761 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     8821 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     8881 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     8941 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     9001 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     9061 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     9121 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     9181 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     9241 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     9301 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     9361 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     9421 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     9481 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
     9541 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
     9601 ttcccgactg g
//
