LOCUS       sequence-270202-        9169 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This plasmid can be used to express independently eGFP and a 
            zebrafish wt1b dominant negative isoform (N-terminal domain) from a 
            bidirectional 5xUAS. Also CFP expression under crystallin promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9169)
  AUTHORS   Sanz-Morejon A, Garcia-Redondo AB, Reuter H, Marques IJ, Bates T, 
            Galardi-Castilla M, Grosse A, Manig S, Langa X, Ernst A, Piragyte I,
            Botos MA, Gonzalez-Rosa JM, Ruiz-Ortega M, Briones AM, Salaices M, 
            Englert C, Mercader N
  TITLE     Wilms Tumor 1b Expression Defines a Pro-regenerative Macrophage 
            Subtype and Is Required for Organ Regeneration in the Zebrafish.
  JOURNAL   Cell Rep. 2019 Jul 30;28(5):1296-1306.e6. doi: 
            10.1016/j.celrep.2019.06.091.
  PUBMED    31365871
REFERENCE   2  (bases 1 to 9169)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2019.06"; journalName: "Cell Rep"; date: 
            "2019-07-30- 30"; volume: "28"; issue: "5"; pages: "1296-1306.e6"
COMMENT     Sequence Label: 
            Tol2-pA-GI-wt1bDNNterm-E1b-5xUAS-E1b-eGFP-GI-pA-Tol2; gcryst:CFP
FEATURES             Location/Qualifiers
     source          1..9169
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    497..630
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    665..799
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    905..1039
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(join(1175..1888,1889..1891,1892..1894))
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     misc_feature    join(2525..2712,2713..2724)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(2758..2774)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2758..2774)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(2811..2831)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(2811..2829)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2850..2866)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2850..2866)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2863..2885)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2874..2890
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2898..2904,2905..2922,2923..2928))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2943..2964
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3081..3098)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3252..3840)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3332..3351)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4011..4802,4803..4871))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4634..4653
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4872..4976)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(5139..5160)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    join(5613..5624,5625..5787)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     regulatory      6180..6189
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      6184..6193
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    6318..6452
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     intron          6525..7097
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     CDS             complement(join(7160..7873,7874..7876,7877..7879))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(7163..7873,7874..7876,7877..7879))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     regulatory      7876..7885
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    7961..8055
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     intron          join(9021..9169,1..424)
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
ORIGIN
        1 ctttctactc tgttgacaac cattgtctcc tcttattttc ttttcatttt ctgtaacttt
       61 ttcgttaaac tttagcttgc atttgtaacg aatttttaaa ttcacttttg tttatttgtc
      121 agattgtaag tactttctct aatcactttt ttttcaaggc aatcagggta tattatattg
      181 tacttcagca cagttttaga gaacaattgt tataattaaa tgataaggta gaatatttct
      241 gcatataaat tctggctggc gtggaaatat tcttattggt agaaacaact acaccctggt
      301 catcatcctg cctttctctt tatggttaca atgatataca ctgtttgaga tgaggataaa
      361 atactctgag tccaaaccgg gcccctctgc taaccatgtt catgccttct tctctttcct
      421 acagctcctg ggcaacgtgc tggttgttgt gctgtctcat cattttggca aagaattaat
      481 tcctcgacgg atcgtagatc cagacatgat aagatacatt gatgagtttg gacaaaccac
      541 aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt
      601 tgtaaccatt ataagctgca ataacaagtt aacaatctag aactatagtg agtcgtatta
      661 cgtagatcca gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca
      721 gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat
      781 aagctgcaat aaacaagtta acaacaattg cattcatttt atgtttcagg ttcaggggga
      841 ggtgtgggag gttttttaat tcgcggccgc ggcgacctgc agcctgttga caattttcga
      901 tgatgatcca gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca
      961 gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat
     1021 aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg
     1081 ggaggtgtgg gaggtttttt aaagcaagta aaacctctac aaatgtggta tggctgatta
     1141 tgatcctcta gatcagatcc agagtcgcgg ccgcttactt gtacagctcg tccatgccga
     1201 gagtgatccc ggcggcggtc acgaactcca gcaggaccat gtgatcgcgc ttctcgttgg
     1261 ggtctttgct cagcttggac tgggtgctca ggtagtggtt gtcgggcagc agcacggggc
     1321 cgtcgccgat gggggtgttc tgctggtagt ggtcggcgag ctgcacgctg ccgtcctcga
     1381 tgttgtggcg gatcttgaag ttggccttga tgccgttctt ctgcttgtcg gcggtgatat
     1441 agacgttgtc gctgatggcg ttgtactcca gcttgtgccc caggatgttg ccgtcctcct
     1501 tgaagtcgat gcccttcagc tcgatgcggt tcaccagggt gtcgccctcg aacttcacct
     1561 cggcgcgggt cttgtagttg ccgtcgtcct tgaagaagat ggtgcgctcc tggacgtagc
     1621 cttcgggcat ggcggacttg aagaagtcgt gctgcttcat gtggtcgggg tagcgggcga
     1681 agcactgcac gccccaggtc agggtggtca cgagggtggg ccagggcacg ggcagcttgc
     1741 cggtggtgca gatgaacttc agggtcagct tgccgtaggt ggcatcgccc tcgccctcgc
     1801 cggacacgct gaacttgtgg ccgtttacgt cgccgtccag ctcgaccagg atgggcacca
     1861 ccccggtgaa cagctcctcg cccttgctca ccatggtacc cccgcaagtc tgactgagtg
     1921 gaagtttcag ttcaatggaa gggagaaaag agccagcctt tatatatgtc atgtattgct
     1981 gctgtctgca tgcgatgaac ctagagctta ctttttctgt caagtaaggc aaaagcagag
     2041 aacctttgtc ttgcattgtg tttgatgttg tgctgtgaca gtcatttctt agttcatgct
     2101 ggtacttttg ttccatagct ttacccaaag agttatccag cattcccagt tattccaaaa
     2161 actcaatgtc acaaattttc cagtccattg cttagcagcc gagatctgcg aagatacggc
     2221 cacgggtgct cttgatcctg tggctgattt tggactgtgc tgctcgcagc tgctgatgaa
     2281 tcacatactt cctccatttt cttccactga ttgactgtta taatttccct aatttccagg
     2341 tcaaggtgct gtgcattgtg gtaatagatg tgacatgacg tcacttccaa aggaccaatg
     2401 aacatgtctg accaatttca tataatgtga aaacgatttt cataggcaga ataaataaca
     2461 tttaaattaa actgggcatc agcgcaattc aattggtttg gtaatagcaa gggaaaatag
     2521 aatgaagtga tctccaaaaa ataagtactt tttgactgta aataaaattg taaggagtaa
     2581 aaagtacttt tttttctaaa aaaatgtaat taagtaaaag taaaagtatt gatttttaat
     2641 tgtactcaag taaagtaaaa atccccaaaa ataatactta agtacagtaa tcaagtaaaa
     2701 ttactcaagt actttacacc tctggttctt gaccccctac cttcagcaag cccagcagat
     2761 ccactagttc tagagcggcc gccaccgcgg tggagctcca gcttttgttc cctttagtga
     2821 gggttaattg cgcgcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
     2881 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
     2941 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     3001 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     3061 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     3121 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     3181 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     3241 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     3301 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     3361 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     3421 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     3481 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     3541 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     3601 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     3661 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     3721 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     3781 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     3841 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     3901 ggcattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     3961 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     4021 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     4081 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     4141 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     4201 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     4261 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     4321 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     4381 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     4441 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     4501 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     4561 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     4621 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     4681 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     4741 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     4801 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     4861 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     4921 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     4981 tttccccgaa aagtgccacc taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa
     5041 atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata
     5101 aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac
     5161 tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc
     5221 cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa
     5281 atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaaggct ggctaagaac
     5341 tcatcagcct ccccggtcca tctacccacg taccaatgca ccaattggcc acaatgacgg
     5401 ctactacatg gtgccattcc ttcctcttta taggaatgga gactacctcc tgtccaacaa
     5461 tgctcttgga tacgagtacg cctacctgtt ggacccaggt cattgcacaa caccagaaat
     5521 gccctctgat ctgcaaaaga cgtgaatatc tgttcagaca cccatatcca ctctgttcca
     5581 cacaggtcag aggtttgtcc aggagttctt gacagaggtg taaaaagtac tcaaaaattt
     5641 tactcaagtg aaagtacaag tacttaggga aaattttact caattaaaag taaaagtatc
     5701 tggctagaat cttacttgag taaaagtaaa aaagtactcc attaaaattg tacttgagta
     5761 ttaaggaagt aaaagtaaaa gcaagaaaga aaactagaga ttcttgttta agcttttaat
     5821 ctcaaaaaac attaaatgaa atgcatacaa ggttttatcc tgctttagaa ctgtttgtat
     5881 ttaattatca aactataaga cagacaatct aatgccagta cacgctactc aaagttgtaa
     5941 aacctcagat ttaacttcag tagaagctga ttctcaaaat tgttagtgtc aagcctagct
     6001 cttttggggc tgaaaagcaa tcctgcagtg ctgaaaagcc tctcacaggc agccgatgcg
     6061 ggaagaggtg tattagtctt gatagagagg ctgcaaatag caggaaacgt gagcagagac
     6121 tccctggtgt ctgaaacaca ggccagatgg gccctcgagc ccgacgtcgc atgctcccgg
     6181 ccgccatggc ggccgcggga attcgattca cacacgccgt accgggccca atgcattggc
     6241 gccgcggccg cgaattaaaa aacctcccac acctccccct gaacctgaaa cataaaatga
     6301 atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     6361 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     6421 aactcatcaa tgtatcttat catgtctgga tctacgatcc gtcgaggaat taattctttg
     6481 ccaaaatgat gagacagcac aacaaccagc acgttgccca ggagctgtag gaaagagaag
     6541 aaggcatgaa catggttagc agaggggccc ggtttggact cagagtattt tatcctcatc
     6601 tcaaacagtg tatatcattg taaccataaa gagaaaggca ggatgatgac cagggtgtag
     6661 ttgtttctac caataagaat atttccacgc cagccagaat ttatatgcag aaatattcta
     6721 ccttatcatt taattataac aattgttctc taaaactgtg ctgaagtaca atataatata
     6781 ccctgattgc cttgaaaaaa aagtgattag agaaagtact tacaatctga caaataaaca
     6841 aaagtgaatt taaaaattcg ttacaaatgc aagctaaagt ttaacgaaaa agttacagaa
     6901 aatgaaaaga aaataagagg agacaatggt tgtcaacaga gtagaaagtg aaagaaacaa
     6961 aattatcatg agggtccatg gtgatacaag ggacatcttc ccattctaaa caacaccctg
     7021 aaaactttgc cccctccata taacatgaat tttacaatag cgaaaaagaa agaacaatca
     7081 agggtcccca aactcaccct gaagttctca ggatcggtcg agtaatacga ctcactatag
     7141 ttctagctag aggctcgagt tacttgtaca gctcgtccat gccgagagtg atcccggcgg
     7201 cggtcacgaa ctccagcagg accatgtgat cgcgcttctc gttggggtct ttgctcaggg
     7261 cggactgggt gctcaggtag tggttgtcgg gcagcagcac ggggccgtcg ccgatggggg
     7321 tgttctgctg gtagtggtcg gcgagctgca cgctgccgtc ctcgatgttg tggcggatct
     7381 tgaagttcac cttgatgccg ttcttctgct tgtcggccat gatatagacg ttgtggctgt
     7441 tgtagttgta ctccagcttg tgccccagga tgttgccgtc ctccttgaag tcgatgccct
     7501 tcagctcgat gcggttcacc agggtgtcgc cctcgaactt cacctcggcg cgggtcttgt
     7561 agttgccgtc gtccttgaag aagatggtgc gctcctggac gtagccttcg ggcatggcgg
     7621 acttgaagaa gtcgtgctgc ttcatgtggt cggggtagcg gctgaagcac tgcacgccgt
     7681 aggtcagggt ggtcacgagg gtgggccagg gcacgggcag cttgccggtg gtgcagatga
     7741 acttcagggt cagcttgccg taggtggcat cgccctcgcc ctcgccggac acgctgaact
     7801 tgtggccgtt tacgtcgccg tccagctcga ccaggatggg caccaccccg gtgaacagct
     7861 cctcgccctt gctcaccatg gtggcggcga attcgtgtgg aggagctcaa agtgaggctg
     7921 agacgcgatg ggatccatta tataccctct agagtctccg ctcggaggac agtactccgc
     7981 tcggaggaca gtactccgct cggaggacag tactccgctc ggaggacagt actccgctcg
     8041 gaggacagta ctccgacctg caggcatgga tctagagggt atataatgga tcccatcgcg
     8101 tctcagcctc actttgagct cctccacacg aattgattta aattaattaa cgccaccatg
     8161 ctgacagagc cgtgcggccc gatgggatcg gatgtcagag acctgagcgc cctgcttcca
     8221 gctgtcccga cacttccagt gggaaactca aactctctgc ctgtcggcga agcttcgcag
     8281 tggggcccga tgctggactt tcaccctgcc tctccctatg gatctcttcc ttcccactcc
     8341 ttcgtgaagc aggagcctgg ctgggggccc ccagaccctt acgaggaccc tcactgcggt
     8401 ttgggggcct tcaccgtaca cttctctggg cagctgacag ggatgcacgg cccatttgct
     8461 gatcctcctt ctagccaaag cagaatgttc tccaatgggc cttatttgcc aggctgcatg
     8521 gagagtcctc ctgctccacg caaccagggt tatgggacag tagcagtcga cggagttccc
     8581 agttacggtc acacacctac acaacacact cctccgttcc ctaatgacac cttcaaacac
     8641 gaggacccga tgtctccgca aagcaccatg ggtgagcagc agttctccgt cccgccgcct
     8701 gtatacggct gtcacagtcc accagatcag acgctgcttc taagaaactc cttcaacagt
     8761 gataatctgt accaaatgag gtctcagctg gagtgtgtgg cctggaatcc tgttagctca
     8821 ctgacgccca gcattaagag ctccagttat gaaactgaag catcttcagg agcacctcct
     8881 ctggtctaca gctgcagcac acagtatcac atacacacac atgggttgtt cagaggatta
     8941 caggatgtgt aagctagcct cgagcctcta gctagaacta tagtgagtcg tattactcga
     9001 ccgatcctga gaacttcagg gtgagtttgg ggacccttga ttgttctttc tttttcgcta
     9061 ttgtaaaatt catgttatat ggagggggca aagttttcag ggtgttgttt agaatgggaa
     9121 gatgtccctt gtatcaccat ggaccctcat gataattttg tttctttca
//
