LOCUS       sequence-270213-       13944 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BeYDV viral replicon on T-DNA backbone expressing Firefly Luc+ and 
            IPT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13944)
  AUTHORS   Maher MF, Nasti RA, Vollbrecht M, Starker CG, Clark MD, Voytas DF
  TITLE     Plant gene editing through de novo induction of meristems.
  JOURNAL   Nat Biotechnol. 2019 Dec 16. pii: 10.1038/s41587-019-0337-2. doi: 
            10.1038/s41587-019-0337-2.
  PUBMED    31844292
REFERENCE   2  (bases 1 to 13944)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2019 Dec 16. pii: 10.1038/s41587-019-0337-2. doi: 
            10.1038/s41587-019-0337-2."
COMMENT     Sequence Label: pMKV059
FEATURES             Location/Qualifiers
     source          1..13944
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(107..695)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(187..206)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(782..1576)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    2001..2025
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             3863..5515
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(3929..3947)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     promoter        6361..6706
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     6649..6669
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     misc_feature    9665..9689
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             10990..11619
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             12048..13121
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      13187..13381
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(13617..13639)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    13725..13865
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     13780..13799
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(13880..13897)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
       61 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
      121 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
      181 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
      241 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
      301 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
      361 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
      421 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
      481 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
      541 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
      601 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
      661 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
      721 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg cattctaggt
      781 actaaaacaa ttcatccagt aaaatataat attttatttt ctcccaatca ggcttgatcc
      841 ccagtaagtc aaaaaatagc tcgacatact gttcttcccc gatatcctcc ctgatcgacc
      901 ggacgcagaa ggcaatgtca taccacttgt ccgccctgcc gcttctccca agatcaataa
      961 agccacttac tttgccatct ttcacaaaga tgttgctgtc tcccaggtcg ccgtgggaaa
     1021 agacaagttc ctcttcgggc ttttccgtct ttaaaaaatc atacagctcg cgcggatctt
     1081 taaatggagt gtcttcttcc cagttttcgc aatccacatc ggccagatcg ttattcagta
     1141 agtaatccaa ttcggctaag cggctgtcta agctattcgt atagggacaa tccgatatgt
     1201 cgatggagtg aaagagcctg atgcactccg catacagctc gataatcttt tcagggcttt
     1261 gttcatcttc atactcttcc gagcaaagga cgccatcggc ctcactcatg agcagattgc
     1321 tccagccatc atgccgttca aagtgcagga cctttggaac aggcagcttt ccttccagcc
     1381 atagcatcat gtccttttcc cgttccacat cataggtggt ccctttatac cggctgtccg
     1441 tcatttttaa atataggttt tcattttctc ccaccagctt atatacctta gcaggagaca
     1501 ttccttccgt atcttttacg cagcggtatt tttcgatcag ttttttcaat tccggtgata
     1561 ttctcatttt agccatttat tatttccttc ctcttttcta cagtatttaa agatacccca
     1621 agaagctaat tataacaaga cgaactccaa ttcactgttc cttgcattct aaaaccttaa
     1681 ataccagaaa acagcttttt caaagttgtt ttcaaagttg gcgtataaca tagtatcgac
     1741 ggagccgatt ttgaaaccgc ggtgatcaca ggcagcaacg ctctgtcatc gttacaatca
     1801 acatgctacc ctccgcgaga tcatccgtgt ttcaaacccg gcagcttagt tgccgttctt
     1861 ccgaatagca tcggtaacat gagcaaagtc tgccgcctta caacggctct cccgctgacg
     1921 ccgtcccgga ctgatgggct gcctgtatcg agtggtgatt ttgtgccgag ctgccggtcg
     1981 gggagctgtt ggctggctgg tggcaggata tattgtggtg taaacaaatt gacgcttaga
     2041 caacttaata acacattgcg gacgttttta atgtagagct caaagtttaa cgcgttagca
     2101 gaaggcatgt tgttgtgact ccgaggggtt gcctcaaact ctatcttata accggcgtgg
     2161 aggcatggag gcaggggtat tttggtcatt ttaatagata gtggaaaatg acgtggaatt
     2221 tacttaaaga cgaagtcttt gcgacaaggg ggggcccacg ccgaatttaa tattaccggc
     2281 gtggcccccc cttatcgcga gtgctttagc acgagcggtc cagatttaaa gtagaaaatt
     2341 tcccgcccac tagggttaaa ggtgttcaca ctataaaagc atatacgatg tgatggtatt
     2401 tgatggagcg tatattgtat caggtatttc cgttggatac gaattattcg tacgaccctc
     2461 ggtaccgatc ggcgcgcgcg gccgcacaga gatttaaata gctccggtga cggacggcgc
     2521 gccctatgtc gagctgcagg tcaacggatc aggatattct tgtttaagat gttgaactct
     2581 atggaggttt gtatgaactg atgatctagg accggataag ttcccttctt catagcgaac
     2641 ttattcaaag aatgttttgt gtatcattct tgttacattg ttattaatga aaaaatatta
     2701 ttggtcattg gactgaacac gagtgttaaa tatggaccag gccccaaata agatccattg
     2761 atatatgaat taaataacaa gaataaatcg agtcaccaaa ccacttgcct tttttaacga
     2821 gacttgttca ccaacttgat acaaaagtca ttatcctatg caaatcaata atcatacaaa
     2881 aatatccaat aacactaaaa aattaaaaga aatggataat ttcacaatat gttatacgat
     2941 aaagaagtta cttttccaag aaattcactg attttataag cccacttgca ttagataaat
     3001 ggcaaaaaaa aacaaaaagg aaaagaaata aagcacgaag aattctagaa aatacgaaat
     3061 acgcttcaat gcagtgggac ccacggttca attattgcca attttcagct ccaccgtata
     3121 tttaaaaaat aaaacgataa tgctaaaaaa atataaatcg taacgatcgt taaatctcaa
     3181 cggctggatc ttatgacgac cgttagaaat tgtggttgtc gacgagtcag taataaacgg
     3241 cgtcaaagtg gttgcagccg gcacacacga gtcgtgttta tcaactcaaa gcacaaatac
     3301 ttttcctcaa cctaaaaata aggcaattag ccaaaaacaa ctttgcgtgt aaacaacgct
     3361 caatacacgt gtcattttat tattagctat tgcttcaccg ccttagcttt ctcgtgacct
     3421 agtcgtcctc gtcttttctt cttcttcttc tataaaacaa tacccaaaga gctcttcttc
     3481 ttcacaattc agatttcaat ttctcaaaat cttaaaaact ttctctcaat tctctctacc
     3541 gtgatcaagg taaatttctg tgttccttat tctctcaaaa tcttcgattt tgttttcgtt
     3601 cgatcccaat ttcgtatatg ttctttggtt tagattctgt taatcttaga tcgaacacga
     3661 ttttctgggt ttgatcgtta gatatcatct taattctcga ttagggtttc atagatatca
     3721 tccgatttgt tcaaataatt tgagttttgt cgaataatta ctcttcgatt tgtgatttct
     3781 atctagatct ggtgttagtt tctagtttgt gcgatcgaat ttgtcgatta atctgagttt
     3841 ttctgattaa caggcctgca ggatggaaga cgccaaaaac ataaagaaag gcccggcgcc
     3901 attctatccg ctggaagatg gaaccgctgg agagcaactg cataaggcta tgaagagata
     3961 cgccctggtt cctggaacaa ttgcttttac agatgcacat atcgaggtgg acatcactta
     4021 cgctgagtac ttcgaaatgt ccgttcggtt ggcagaagct atgaaacgat atgggctgaa
     4081 tacaaatcac agaatcgtcg tatgcagtga aaactctctt caattcttta tgccggtgtt
     4141 gggcgcgtta tttatcggag ttgcagttgc gcccgcgaac gacatttata atgaacgtga
     4201 attgctcaac agtatgggca tttcgcagcc taccgtggtg ttcgtttcca aaaaggggtt
     4261 gcaaaaaatt ttgaacgtgc aaaaaaagct cccaatcatc caaaaaatta ttatcatgga
     4321 ttctaaaacg gattaccagg gatttcagtc gatgtacacg ttcgtcacat ctcatctacc
     4381 tcccggtttt aatgaatacg attttgtgcc agagtccttc gatagggaca agacaattgc
     4441 actgatcatg aactcctctg gatctactgg tctgcctaaa ggtgtcgctc tgcctcatag
     4501 aactgcctgc gtgagattct cgcatgccag agatcctatt tttggcaatc aaatcattcc
     4561 ggatactgcg attttaagtg ttgttccatt ccatcacggt tttggaatgt ttactacact
     4621 cggatatttg atatgtggat ttcgagtcgt cttaatgtat agatttgaag aagagctgtt
     4681 tctgaggagc cttcaggatt acaagattca aagtgcgctg ctggtgccaa ccctattctc
     4741 cttcttcgcc aaaagcactc tgattgacaa atacgattta tctaatttac acgaaattgc
     4801 ttctggtggc gctcccctct ctaaggaagt cggggaagcg gttgccaaga ggttccatct
     4861 gccaggtatc aggcaaggat atgggctcac tgagactaca tcagctattc tgattacacc
     4921 cgagggggat gataaaccgg gcgcggtcgg taaagttgtt ccattttttg aagcgaaggt
     4981 tgtggatctg gataccggga aaacgctggg cgttaatcaa agaggcgaac tgtgtgtgag
     5041 aggtcctatg attatgtccg gttatgtaaa caatccggaa gcgaccaacg ccttgattga
     5101 caaggatgga tggctacatt ctggagacat agcttactgg gacgaagacg aacacttctt
     5161 catcgttgac cgcctgaagt ctctgattaa gtacaaaggc tatcaggtgg ctcccgctga
     5221 attggaatcc atcttgctcc aacaccccaa catcttcgac gctggtgtcg caggtcttcc
     5281 cgacgatgac gccggtgaac ttcccgccgc cgttgttgtt ttggagcacg gaaagacgat
     5341 gacggaaaaa gagatcgtgg attacgtcgc cagtcaagta acaaccgcga aaaagttgcg
     5401 cggaggagtt gtgtttgtgg acgaagtacc gaaaggtctt accggaaaac tcgacgcaag
     5461 aaaaatcaga gagatcctca taaaggccaa gaagggcgga aagatcgccg tgtgactcga
     5521 ggttcgagta ttatggcatt gggaaaactg tttttcttgt accatttgtt gtgcttgtaa
     5581 tttactgtgt tttttattcg gttttcgcta tcgaactgtg aaatggaaat ggatggagaa
     5641 gagttaatga atgatatggt ccttttgttc attctcaaat taatattatt tgttttttct
     5701 cttatttgtt gtgtgttgaa tttgaaatta taagagatat gcaaacattt tgttttgagt
     5761 aaaaatgtgt caaatcgtgg cctctaatga ccgaagttaa tatgaggagt aaaacacttg
     5821 tagttgtgtt agagctcccg gggcgcgccg atatcgagct ctcccggcgc gccagatttg
     5881 ccttttcaat ttcagaaaga atgctaaccc acagatggtt agagaggctt acgcagcagg
     5941 tatcatcaag acgatctacc cgagcaataa tctccaggaa atcaaatacc ttcccaagaa
     6001 ggttaaagat gcagtcaaaa gattcaggac taactgcatc aagaacacag agaaagatat
     6061 atttctcaag atcagaagta ctattccagt atggacgatt caaggcttgc ttcacaaacc
     6121 aaggcaagta atagagattg gagtctctaa aaaggtagtt cccactgaat caaaggccat
     6181 ggagtcaaag attcaaatag aggacctaac agaactcgcc gtaaagactg gcgaacagtt
     6241 catacagagt ctcttacgac tcaatgacaa gaagaaaatc ttcgtcaaca tggtggagca
     6301 cgacacactt gtctactcca aaaatatcaa agatacagtc tcagaagacc aaagggcaat
     6361 tgagactttt caacaaaggg taatatccgg aaacctcctc ggattccatt gcccagctat
     6421 ctgtcacttt attgtgaaga tagtggaaaa ggaaggtggc tcctacaaat gccatcattg
     6481 cgataaagga aaggccatcg ttgaagatgc ctctgccgac agtggtccca aagatggacc
     6541 cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt
     6601 ggattgatgt gatatctcca ctgacgtaag ggatgacgca caatcccact atccttcgca
     6661 agacccttcc tctatataag gaagttcatt tcatttggag agaacacggg ggactcctgc
     6721 aggatggatc tgcgtctaat tttcggtcca acttgcacag gaaagacgtc gaccgcgata
     6781 cgtcttgccc agcagactgg ccttccagtc ctttcgctcg atcgggtcca atgctgtcct
     6841 caactgtcaa ccggaagcgg acgaccaaca gtggaagaac tgaaaggaac gacccgtcta
     6901 taccttgaag atcggcctct ggtgaagggt atcatcgcag ccaagcaagc tcacgaaagg
     6961 ctgatcgggg aagtgtacaa ttatgaggcc cacggcgggc ttattcttga gggaggatct
     7021 atctcgttgc tcaggtgcat ggcgcaaagc agttattgga gtaccgattt tcgttggcat
     7081 attattcgcc acaagttagc agacgaggag acattcatga acgcggccaa ggccagagtt
     7141 aggcagatgt tgcgccctgc tgtaggccca tctattattc aagagttggt tcatctttgg
     7201 aatgagcctc ggctgaggcc catactgaaa gagatcgacg gatatcgata tgccatgtta
     7261 tttgctagcc agaaccagat cacacccgat atgctattgc agcttgaccc agatatggag
     7321 ggtgagttga ttcatggaat cgctcaggag tatctcatcc atgcgcgccg gcaggagcag
     7381 gaattccctc cagtgagcgt ggtcgctttc gaaggattcg aaggtccacc gttcggaatg
     7441 tgctagctcg agccctagac ttgtccatct tctggattgg ccaagttaat taatgtatga
     7501 aataaaagga tgcacacata gtgacatgct aatcactata atgtgggcat caaagttgtg
     7561 tgttatgtgt aattactaat tatctgaata agagaaagag atcatccata tttcttatcc
     7621 taaatgaatg tcacgtgtct ttataattct ttgatgaacc agatgcattt tattaaccaa
     7681 ttccatatac atataaatat taatcatata taattaatat caattgggtt agcaaaacaa
     7741 atctagtcta ggtgtgtttt gctaattatt gggggatagt gcaaaaagaa atctacgttc
     7801 tcaataattc agatagaaaa cttaataaag tgagataatt tacatagatt gcttttatcc
     7861 tttgatatat gtgaaaccat gcatgatata aggaaaatag atagagaaat aattttttac
     7921 atcgttgaat atgtaaacaa tttaattcaa gaagctagga atataaatat tgaggagttt
     7981 atgattagag ctcagtgttt gatcgccggc ggtaccgagt gtacttcaag tcagtgggaa
     8041 atcaataaaa tgattatttt atgaatatat ttcattgtgc aagtagatag aaattacata
     8101 tgttacataa cacacgaaat aaacaaaaaa agacaatcca aaaacaaaca ccccaaaaaa
     8161 aataatcact ttagataaac tcgtatgagg agaggcacgt tcagtgactc gacgattccc
     8221 gagcaaaaaa agtctccccg tcacacatgt agtgggtgac gcaattatct ttaaagtaat
     8281 ccttctgttg acttgtcatt gataacatcc agtcttcgtc aggattgcaa agaattatag
     8341 aagggatccc accttttatt ttcttctttt ttccatattt agggttgaca gtgaaatcag
     8401 actggcaacc tattaattgc ttccacaatg ggacgaactt gaaggggatg tcgtcgatga
     8461 tattataggt ggcgtgttca tcgtagttgg tgaaatcgat ggtaccgttc caatagttgt
     8521 gtcgtccgag acttctagcc caggtggtct ttccggtacg agttggtccg cagatgtaga
     8581 ggctggggtg tcggattcca ttccttccat tgtccttgtt aaatcggcca tccattcaag
     8641 gtcagattga gcttgttggt atgagacagg atgtatgtaa gtataagcgt ctatgcttac
     8701 atggtataga tgggtttccc tccaggagtg tagatcttcg tggcagcgaa gatctgattc
     8761 tgtgaagggc gacacatacg gttcaggttg tggagggaat aatttgttgg ctgaatattc
     8821 cagccattga agctttgttg cccattcatg agggaattct tccttgatca tgtcaagata
     8881 ttcctcctta gacgttgcag tctggataat agttctccat cgtgcgtcag atttgcgagg
     8941 agaaacctta tgatctcgga aatctcctct ggttttaata tctccgtcct ttgatatgta
     9001 atcaaggact tgtttagagt ttctagctgg ctggatatta gggtgatttc cttcaaaatc
     9061 gaaaaaagaa ggatccctaa tacaaggttt tttatcaagc tggagaagag catgatagtg
     9121 ggtagtgcca tcttgatgaa gctcagaagc aacaccaagg aagaaaataa gaaaaggtgt
     9181 gagtttctcc cagagaaact ggaataaatc atctctttga gatgagcact tgggataggt
     9241 aaggaaaaca tatttagatt ggagtctgaa gttcttacta gcagaaggca tgttgttgtg
     9301 actccgaggg gttgcctcaa actctatctt ataaccggcg tggaggcatg gaggcagggg
     9361 tattttggtc attttaatag atagtggaaa atgacgtgga atttacttaa agacgaagtc
     9421 tttgcgacaa gggggggccc acgccgaatt taatattacc ggcgtggccc ccccttatcg
     9481 cgagtgcttt agcacgagcg gtccagattt aaagtagaaa atttcccgcc cactagggtt
     9541 aaaggtgttc acactataaa agcatatacg atgtgatggt atttgatgga gcgtatattg
     9601 tatcaggtat ttccgttgga tacgaattat tcgtacgacc ctcatagttt aaactatcag
     9661 tgtttgacag gatatattgg cgggtaaacc taagagaaaa gagcgtttat tagaataatc
     9721 ggatatttaa aagggcgtga aaaggtttat ccgttcgtcc atttgtatgt gcatgccaac
     9781 cacagggttc ccctcgggat caaagtactt tgatccaacc cctccgctgc tatagtgcag
     9841 tcggcttctg acgttcagtg cagccgtctt ctgaaaacga catgtcgcac aagtcctaag
     9901 ttacgcgaca ggctgccgcc ctgccctttt cctggcgttt tcttgtcgcg tgttttagtc
     9961 gcataaagta gaatacttgc gactagaacc ggagacatta cgccatgaac aagagcgccg
    10021 ccgctggcct gctgggctat gcccgcgtca gcaccgacga ccaggacttg accaaccaac
    10081 gggccgaact gcacgcggcc ggctgcacca agctgttttc cgagaagatc accggcacca
    10141 ggcgcgaccg cccggagctg gccaggatgc ttgaccacct acgccctggc gacgttgtga
    10201 cagtgaccag gctagaccgc ctggcccgca gcacccgcga cctactggac attgccgagc
    10261 gcatccagga ggccggcgcg ggcctgcgta gcctggcaga gccgtgggcc gacaccacca
    10321 cgccggccgg ccgcatggtg ttgaccgtgt tcgccggcat tgccgagttc gagcgttccc
    10381 taatcatcga ccgcacccgg agcgggcgcg aggccgccaa ggcccgaggc gtgaagtttg
    10441 gcccccgccc taccctcacc ccggcacaga tcgcgcacgc ccgcgagctg atcgaccagg
    10501 aaggccgcac cgtgaaagag gcggctgcac tgcttggcgt gcatcgctcg accctgtacc
    10561 gcgcacttga gcgcagcgag gaagtgacgc ccaccgaggc caggcggcgc ggtgccttcc
    10621 gtgaggacgc attgaccgag gccgacgccc tggcggccgc cgagaatgaa cgccaagagg
    10681 aacaagcatg aaaccgcacc aggacggcca ggacgaaccg tttttcatta ccgaagagat
    10741 cgaggcggag atgatcgcgg ccgggtacgt gttcgagccg cccgcgcacg gctcaaccgt
    10801 gcggctgcat gaaatcctgg ccggtttgtc tgatgccaag ctggcggcct ggccggccag
    10861 cttggccgct gaagaaaccg agcgccgccg tctaaaaagg tgatgtgtat ttgagtaaaa
    10921 cagcttgcgt catgcggtcg ctgcgtatat gatgcgatga gtaaataaac aaatacgcaa
    10981 ggggaacgca tgaaggttat cgctgtactt aaccagaaag gcgggtcagg caagacgacc
    11041 atcgcaaccc atctagcccg cgccctgcaa ctcgccgggg ccgatgttct gttagtcgat
    11101 tccgatcccc agggcagtgc ccgcgattgg gcggccgtgc gggaagatca accgctaacc
    11161 gttgtcggca tcgaccgccc gacgattgac cgcgacgtga aggccatcgg ccggcgcgac
    11221 ttcgtagtga tcgacggagc gccccaggcg gcggacttgg ctgtgtccgc gatcaaggca
    11281 gccgacttcg tgctgattcc ggtgcagcca agcccttacg acatatgggc caccgccgac
    11341 ctggtggagc tggttaagca gcgcattgag gtcacggatg gaaggctaca agcggccttt
    11401 gtcgtgtcgc gggcgatcaa aggcacgcgc atcggcggtg aggttgccga ggcgctggcc
    11461 gggtacgagc tgcccattct tgagtcccgt atcacgcagc gcgtgagcta cccaggcact
    11521 gccgccgccg gcacaaccgt tcttgaatca gaacccgagg gcgacgctgc ccgcgaggtc
    11581 caggcgctgg ccgctgaaat taaatcaaaa ctcatttgag ttaatgaggt aaagagaaaa
    11641 tgagcaaaag cacaaacacg ctaagtgccg gccgtccgag cgcacgcagc agcaaggctg
    11701 caacgttggc cagcctggca gacacgccag ccatgaagcg ggtcaacttt cagttgccgg
    11761 cggaggatca caccaagctg aagatgtacg cggtacgcca aggcaagacc attaccgagc
    11821 tgctatctga atacatcgcg cagctaccag agtaaatgag caaatgaata aatgagtaga
    11881 tgaattttag cggctaaagg aggcggcatg gaaaatcaag aacaaccagg caccgacgcc
    11941 gtggaatgcc ccatgtgtgg aggaacgggc ggttggccag gcgtaagcgg ctgggttgcc
    12001 tgccggccct gcaatggcac tggaaccccc aagcccgagg aatcggcgtg agcggtcgca
    12061 aaccatccgg cccggtacaa atcggcgcgg cgctgggtga tgacctggtg gagaagttga
    12121 aggccgcgca ggccgcccag cggcaacgca tcgaggcaga agcacgcccc ggtgaatcgt
    12181 ggcaagcggc cgctgatcga atccgcaaag aatcccggca accgccggca gccggtgcgc
    12241 cgtcgattag gaagccgccc aagggcgacg agcaaccaga ttttttcgtt ccgatgctct
    12301 atgacgtggg cacccgcgat agtcgcagca tcatggacgt ggccgttttc cgtctgtcga
    12361 agcgtgaccg acgagctggc gaggtgatcc gctacgagct tccagacggg cacgtagagg
    12421 tttccgcagg gccggccggc atggccagtg tgtgggatta cgacctggta ctgatggcgg
    12481 tttcccatct aaccgaatcc atgaaccgat accgggaagg gaagggagac aagcccggcc
    12541 gcgtgttccg tccacacgtt gcggacgtac tcaagttctg ccggcgagcc gatggcggaa
    12601 agcagaaaga cgacctggta gaaacctgca ttcggttaaa caccacgcac gttgccatgc
    12661 agcgtacgaa gaaggccaag aacggccgcc tggtgacggt atccgagggt gaagccttga
    12721 ttagccgcta caagatcgta aagagcgaaa ccgggcggcc ggagtacatc gagatcgagc
    12781 tagctgattg gatgtaccgc gagatcacag aaggcaagaa cccggacgtg ctgacggttc
    12841 accccgatta ctttttgatc gatcccggca tcggccgttt tctctaccgc ctggcacgcc
    12901 gcgccgcagg caaggcagaa gccagatggt tgttcaagac gatctacgaa cgcagtggca
    12961 gcgccggaga gttcaagaag ttctgtttca ccgtgcgcaa gctgatcggg tcaaatgacc
    13021 tgccggagta cgatttgaag gaggaggcgg ggcaggctgg cccgatccta gtcatgcgct
    13081 accgcaacct gatcgagggc gaagcatccg ccggttccta atgtacggag cagatgctag
    13141 ggcaaattgc cctagcaggg gaaaaaggtc gaaaaggcct ctttcctgtg gatagcacgt
    13201 acattgggaa cccaaagccg tacattggga accggaaccc gtacattggg aacccaaagc
    13261 cgtacattgg gaaccggtca cacatgtaag tgactgatat aaaagagaaa aaaggcgatt
    13321 tttccgccta aaactcttta aaacttatta aaactcttaa aacccgcctg gcctgtgcat
    13381 aactgtctgg ccagcgcaca gccgaagagc tgcaaaaagc gcctaccctt cggtcgctgc
    13441 gctccctacg ccccgccgct tcgcgtcggc ctatcgcggc cgctggccgc tcaaaaatgg
    13501 ctggcctacg gccaggcaat ctaccagggc gcggacaagc cgcgccgtcg ccactcgacc
    13561 gccggcgccc acatcaaggc accctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc
    13621 tgacacatgc agctcccgga aacggtcaca gcttgtctgt aagcggatgc cgggagcaga
    13681 caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc catgacccag
    13741 tcacgtagcg atagcggagt gtatactggc ttaactatgc ggcatcagag cagattgtac
    13801 tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca
    13861 tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
    13921 gagcggtatc agctcactca aagg
//
