LOCUS       sequence-270220-        6680 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses ANXA2 chimeras in bacterial cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6680)
  AUTHORS   Ecsedi P, Gogl G, Hof H, Kiss B, Harmat V, Nyitray L
  TITLE     Structure Determination of the Transactivation Domain of p53 in 
            Complex with S100A4 Using Annexin A2 as a Crystallization Chaperone.
  JOURNAL   Structure. 2020 Aug 4;28(8):943-953.e4. doi: 
            10.1016/j.str.2020.05.001. Epub 2020 May 21.
  PUBMED    32442400
REFERENCE   2  (bases 1 to 6680)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.str.2020.05.001"; journalName: "Structure"; date: 
            "2020-08-4- 4"; volume: "28"; issue: "8"; pages: "943-953.e4"
COMMENT     Sequence Label: NdeI – SacII – SpeI(NheI) - ANXA2 (23-339)
FEATURES             Location/Qualifiers
     source          1..6680
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1..18
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             40..60
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     primer_bind     complement(1089..1107)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      1103..1150
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(join(1325..1330,1331..1347,1348..1353))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(1380..1398)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1466..1570
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1571..1639,1640..2431)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1789..1808)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2602..3190
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3091..3110
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3344..3361
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    3376..3515
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(3441..3460)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3601..3623
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(3617..3808)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     6163..6182
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(6202..6279)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     6485..6504
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     6588..6607
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6588..6606
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    6607..6631
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             6646..6668
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 catcaccatc accatcacga ctacgacatc ccgactaccg aaaacctgta cttccaggga
       61 tcgcatatgc cgcggactag tgcttatggg tctgtcaaag cctatactaa ctttgatgct
      121 gagcgggatg ctttgaacat tgaaacagcc atcaagacca aaggtgtgga tgaggtcacc
      181 attgtcaaca ttttgaccaa ccgcagcaat gaacagagac aggatattgc cttcgcctac
      241 cagagaagga ccaaaaagga acttgcatca gcactgaagt cagccttatc tggccacctg
      301 gagacggtga ttttgggcct attgaagaca cctgctcagt atgacgcttc tgagctaaag
      361 gcttccatga aggggctggg aaccgacgag gactctctca ttgagatcat ctgctccaga
      421 accaaccagg agctgcagga aattaacaga gtctacaagg aaatgtacaa gactgatctg
      481 gagaaggaca ttatttcgga cacatctggt gacttccgca agctgatggt tgccctggca
      541 aagggtagaa gagcagagga tggctctgtc attgattatg aactgattga ccaagatgct
      601 cgggatctct atgacgctgg agtgaagagg aaaggaactg atgttcccaa gtggatcagc
      661 atcatgaccg agcggagcgt gccccacctc cagaaagtat ttgataggta caagagttac
      721 agcccttatg acatgttgga aagcatcagg aaagaggtta aaggagacct ggaaaatgct
      781 ttcctgaacc tggttcagtg cattcagaac aagcccctgt attttgctga tcggctgtat
      841 gactccatga agggcaaggg gacgcgagat aaggtcctga tcagaatcat ggtctcccgc
      901 agtgaagtgg acatgttgaa aattaggtct gagttcaaga gaaagtacgg caagtccctg
      961 tactattata tccagcaaga cactaagggc gactaccaga aagcgctgct gtacctgtgt
     1021 ggtggagatg actaactcga atccggctgc taacaaagcc cgaaaggaag ctgagttggc
     1081 tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag
     1141 gggttttttg ctgaaaggag gaactatatc cggatatccc gcaagaggcc cggcagtacc
     1201 ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc
     1261 gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta
     1321 ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg
     1381 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc
     1441 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     1501 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     1561 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     1621 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     1681 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     1741 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     1801 ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     1861 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     1921 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     1981 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     2041 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     2101 caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     2161 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     2221 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     2281 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     2341 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     2401 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     2461 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     2521 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     2581 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     2641 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     2701 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta
     2761 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     2821 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     2881 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     2941 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     3001 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     3061 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     3121 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     3181 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     3241 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     3301 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     3361 ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca
     3421 ccgcaatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca
     3481 ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg
     3541 acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct
     3601 ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc
     3661 ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt
     3721 ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt
     3781 taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca
     3841 tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg
     3901 aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg
     3961 accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc
     4021 cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg
     4081 acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc
     4141 aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat
     4201 tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga
     4261 tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg
     4321 agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc
     4381 gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc
     4441 ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac
     4501 aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc
     4561 ataaatcgcc gtgacgatca gcggtccaat gatcgaagtt aggctggtaa gagccgcgag
     4621 cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa
     4681 cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc
     4741 tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat
     4801 ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc
     4861 gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg
     4921 gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa
     4981 gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac
     5041 tgggttgaag gctctcaagg gcatcggtcg agatcccggt gcctaatgag tgagctaact
     5101 tacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaactgtc gtgccagctg
     5161 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ccagggtggt
     5221 ttttcttttc accagtgaga cgggcaacag ctgattgccc ttcaccgcct ggccctgaga
     5281 gagttgcagc aagcggtcca cgctggtttg ccccagcagg cgaaaatcct gtttgatggt
     5341 ggttaacggc gggatataac atgagctgtc ttcggtatcg tcgtatccca ctaccgagat
     5401 atccgcacca acgcgcagcc cggactcggt aatggcgcgc attgcgccca gcgccatctg
     5461 atcgttggca accagcatcg cagtgggaac gatgccctca ttcagcattt gcatggtttg
     5521 ttgaaaaccg gacatggcac tccagtcgcc ttcccgttcc gctatcggct gaatttgatt
     5581 gcgagtgaga tatttatgcc agccagccag acgcagacgc gccgagacag aacttaatgg
     5641 gcccgctaac agcgcgattt gctggtgacc caatgcgacc agatgctcca cgcccagtcg
     5701 cgtaccgtct tcatgggaga aaataatact gttgatgggt gtctggtcag agacatcaag
     5761 aaataacgcc ggaacattag tgcaggcagc ttccacagca atggcatcct ggtcatccag
     5821 cggatagtta atgatcagcc cactgacgcg ttgcgcgaga agattgtgca ccgccgcttt
     5881 acaggcttcg acgccgcttc gttctaccat cgacaccacc acgctggcac ccagttgatc
     5941 ggcgcgagat ttaatcgccg cgacaatttg cgacggcgcg tgcagggcca gactggaggt
     6001 ggcaacgcca atcagcaacg actgtttgcc cgccagttgt tgtgccacgc ggttgggaat
     6061 gtaattcagc tccgccatcg ccgcttccac tttttcccgc gttttcgcag aaacgtggct
     6121 ggcctggttc accacgcggg aaacggtctg ataagagaca ccggcatact ctgcgacatc
     6181 gtataacgtt actggtttca cattcaccac cctgaattga ctctcttccg ggcgctatca
     6241 tgccataccg cgaaaggttt tgcgccattc gatggtgtcc gggatctcga cgctctccct
     6301 tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg
     6361 ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg
     6421 ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc
     6481 catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg
     6541 ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaattaa tacgactcac
     6601 tataggggaa ttgtgagcgg ataacaattc ccctctagaa ataattttgt ttaactttaa
     6661 gaaggagata taccatgggc
//
