LOCUS       sequence-270223-       16953 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  B6J Cdk6-T2A-td-sfGFP allele targeting vector, low copy number.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16953)
  AUTHORS   Nam HS, Capecchi MR
  TITLE     Lrig1 expression prospectively identifies stem cells in the 
            ventricular-subventricular zone that are neurogenic throughout adult
            life.
  JOURNAL   Neural Dev. 2020 Mar 17;15(1):3. doi: 10.1186/s13064-020-00139-5.
  PUBMED    32183906
REFERENCE   2  (bases 1 to 16953)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neural 
            Dev."; date: "2020-03-17"; volume: "15(1):3. doi"; pages: " 
            10.1186/s13064-020-00139-5"
COMMENT     Sequence Label: pB6-Cdk6-T2A-td-sfGFP-FNF-TK
FEATURES             Location/Qualifiers
     source          1..16953
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     41..58
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             4381..4434
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(4444..4446,4447..5157)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             4444..5157
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Nager et al., 2011)"
                     /label=superfolder GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             join(5188..5190,5191..5904)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             5188..5901
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Nager et al., 2011)"
                     /label=superfolder GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     protein_bind    complement(5937..5970)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     polyA_signal    6025..6249
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     6238..6255
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(6287..7090)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6398..6417)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     7008..7027
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(7109..7156)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(7168..7667)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(7216..7235)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     7609..7632
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     protein_bind    complement(7731..7764)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     promoter        13047..13109
                     /label=Mini-TK promoter
                     /note="minimal herpes simplex virus (HSV) thymidine kinase 
                     promoter"
     5'UTR           13131..13170
                     /label=HSV TK 5'-UTR
                     /note="5' untranslated region from the herpes simplex virus
                     thymidine kinase gene"
     CDS             13188..14318
                     /codon_start=1
                     /product="herpes simplex virus thymidine kinase"
                     /label=HSV TK
                     /note="confers sensitivity to nucleoside analogs such as 
                     acyclovir or ganciclovir"
                     /translation="MASYPGHQHASAFDQAARSRGHSNRRTALRPRRQQEATEVRPEQK
                     MPTLLRVYIDGPHGMGKTTTTQLLVALGSRDDIVYVPEPMTYWRVLGASETIANIYTTQ
                     HRLDQGEISAGDAAVVMTSAQITMGMPYAVTDAVLAPHIGGEAGSSHAPPPALTLIFDR
                     HPIAALLCYPAARYLMGSMTPQAVLAFVALIPPTLPGTNIVLGALPEDRHIDRLAKRQR
                     PGERLDLAMLAAIRRVYGLLANTVRYLQCGGSWREDWGQLSGTAVPPQGAEPQSNAGPR
                     PHIGDTLFTLFRAPELLAPNGDLYNVFAWALDVLAKRLRSMHVFILDYDQSPAGCRDAL
                     LQLTSGMVQTHVTTPGSIPTICDLARTFAREMGEAN"
     primer_bind     complement(14316..14335)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    14360..14408
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     complement(14712..14729)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(14712..14728)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(14721..14743)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(14937..14956)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     15056..15078
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(15116..15134)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        15202..15306
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(15307..15375,15376..16167)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(15525..15544)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      16332..16877
                     /direction=RIGHT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     16778..16797
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gtaagccatt tccgctcgcc gcagtcgaac gaccgagcgt agcgagtcag tgagcgagga
       61 agcggaatat atcctgtatc acatattctg ctgacgcacc ggtgcagcct tttttctcct
      121 gccacatgaa gcacttcact gacaccctca tcagtgccaa catagtaagc cagtatacac
      181 tccgctagca acctggctta tcgaaatatt taaatggagg ccatcatctg ctctccattt
      241 cttatgcctg gtgggattcc tagccacctc cagcacccag tctctggacc tgtggcatta
      301 cgtgtactgc cacaaagcca atgcagtagg acagaaggga ctttttgctt gttccgtggg
      361 aaagcctgct tgacttttgg ccaaggcctg gcactcccac atagctaaca gaagagcagc
      421 tggaaagcct tgtatgtttg gtgcctttag attatactgg ctttgtttca tttctctgaa
      481 cagtgctctc aacgggaaaa ggacaatgct gcaccctagt gtcactgcta acccacttca
      541 ttatttccac cacagccatt ttacagtctg tgccctgtag ttcttccccg tgtaaacagt
      601 gaacggttac agattctatc caaccgtcca aatagagaat tatgatgcag gccttatcga
      661 atgaacctgt catgatttca ttatgctcat taagggccat gacagtcctt attggacagg
      721 aagctgattt tttttttcct aaggaaagtc atagtctcag actcttaaca aggtaagctg
      781 tagacacatt tctactttca gagagcattg agtgcttcag aaggaaagca gttaggccat
      841 gacatttaga gcgttttcta agaccagtat catagttaag tggaggtata aaaaaaaaga
      901 tgctgaacat ggagtgagga gaaccgaggc tgtccgcaca gtgtgtgacc tgggcaggca
      961 ttctccttcc tcattttgtc ttccatccct acagtgaggt ggcacacctg acctatgtgt
     1021 ctctcacagc tttttacagg agatggagaa gacaacaggc tctaagtttt attcgtatgt
     1081 atttgtgtat gtgtgtatgt gtgtgatatg tatccatgca ttaggtgtgg ttaatatgta
     1141 tttacatatc agactggcag acagactgtt tccctgaaag cgtctggctt ctgactgctg
     1201 tattcataag gtttcaaatc agcaagtatg ttcaaagcta ctaataacac tcaggaggct
     1261 gagacaggag gattttcagt tggagaccag cctgggatac ctagtgaaac actatctcaa
     1321 aagaaaagat gctgtctgtc aataaagtga tttaaagtct aagcatgatt acaatccgtt
     1381 cctaaggggc tgataatgag gatgtactaa agtccttgta caaaagggca cacatttcaa
     1441 actgtttgtc agccatatcc catactaccc aaacccatcc ccatgttact tttcgtgatt
     1501 atgacaacat ttttacccct tcattaccca ctatagacct ggctattgag cacacttata
     1561 tgatgcccaa gtcatgattt tgctcaatga ttccaggaag tctatccatg agtctttgta
     1621 tgcctgagta atgtaactct ctgcagatgc ccagtgggga cagagttagt gtcttgcctc
     1681 ggcacccagg ggcatgtgcg tgggtgatgg cgaagctgct ctttccaggg aggtctgtga
     1741 cctgggctct gtaaagggaa gggaacagtg ataaagccct gtgacattta cctgtcacat
     1801 tatattatcc tgtgacattt accacctcct ttcctaaaag atgcttgtga ccacgctttc
     1861 taggcctgcg cgtcaggaag tcagaacaga accttaattc aggccagcgc catttctagt
     1921 tagggttaaa atagtttgga tcattatcta ggtcaccata tatcctgtcc gctcaactgt
     1981 gattttgtct tcttttagtt cagagttact gagtctgtac cgtccaaata cacttagcac
     2041 ctatacataa actataaata atactacacc tggataaatc atatagggcc acctgcttac
     2101 cattaataag taacaaatgg tactgtaatg tgcagtttca gtaaattacc gccaaccctc
     2161 attgtaacca taaattattg ttgtcacgta cataaagaca ccagggctca tagttgtcat
     2221 gtaatttgtc cttacctgtc aattaagggc aggtgctgaa tttgaagcca cgtccgccta
     2281 tttccaaagc tcttttatga ctcgctgttt cctccacatt tctgatccca gaatggtagt
     2341 caggggggaa tctaaatgat accctttcta attttctgtt tctcactctg gaataccatt
     2401 acacagcgct tccataagac aggcccatcg tagtttttaa ccttatcttt gagtctaatt
     2461 tatgtaatgg tttagaatat tgctctttaa tttacgaacc tcagccaaag tgatgcgtgt
     2521 cacaagccgt gccggacgag ctgcctgaaa acacattttc acagaactgt tgtatcacac
     2581 cacaaagcga agcttcgggt gtaggtgaag acttggtata tctgatgagt gtccttcaga
     2641 atggcagacc ctgccttgtc actacaccaa gacttacacc aggggggcgc cctgccctgt
     2701 cactaagccg ggacttctcc ctggctgcac cagtgccatt cacatacaca ccttccatcg
     2761 ggggtctgct gccagggaca gacacttcct cttgtcagtg aagtttgcag tagggtaaat
     2821 gagaagtcct ttctgccatg aggtggggtt ctccctgtgc cgctctaaga tttggtaagg
     2881 aaggtcatgt ttaaaccgca ggcatttaca ttcccgtctg gttcttccgt agcatcattg
     2941 gactcccagg agaggaagac tggcctaggg acgtggccct tccccggcag gcttttcatt
     3001 ccaaatctgc tcaacccatc gagaagtttg tgacagatat tgacgaacta ggcaaagacc
     3061 tacttctggt aagttcctgg gatgccgtca ccttgtgccc caaccatgtg gcaacgacgt
     3121 ggcgtgggag gctgtatttg tttttgtgga ttccgtttcc agctggtgag ttttgattct
     3181 gcaatgtctt cctaacccag ctctcaacac ttgaatgtga atttggacga agctttatga
     3241 gacgctaggt tccttgggca aaggcttctt gacaagaaat tgcttttctt cgcttgaagt
     3301 ttcatgccac ttgaagcaac gtgctagtcg catctttgca aaatatttcc ccccaaagga
     3361 accaagtaac catacttgta gttattgatt tactgaataa gtaccttgtg tgctattcac
     3421 atataagata tcccattaaa aacaagatcc tagggctgga gaggtgtctc cgttctcaag
     3481 aatatgtatc atttttgcag agggcccaag ttcagttctc agcacctgta cccagcagct
     3541 tacagcagcc tggaactcta gctccagaga cactcttggc cccacaggcg cttgggttca
     3601 aacgcacatt cccacacaca gagacgcgaa tacttattct taaaatgaag tttttttttt
     3661 tttaataagc acttttgtat tttgaaactc aaactcctcg ttgttggtgt tgtgtataat
     3721 gtccttcctg cagacacaga aggccctctt ccttctgttc tcagagtcag agccctatga
     3781 agaataagtc tgttattgat cctgaattag gagcccgtac tcttgattca gaccgtgggt
     3841 ctatcaaacc tgcatgtgtc tgtgtgtctc accctgcctg gtggcatgtt tttctcatgg
     3901 aggcaagtag aaccatgacc aaaggcctag gtcagttatg tggtcagggc agcttgaaga
     3961 aaggtgagaa tgttcaaccc ttgagtaagt tgtgatacta gaaggtaatt ttaagtcagg
     4021 tgtctggtaa tcacattaat tgtttttacg gaagcagcac ttcttgtgtc ggggtgtttt
     4081 actttagttc atgtcgtgct ggtgactttt ctccccaaat agaactgtgg aaaaatttta
     4141 gccacctttt ccaagacttc ccccaaggtc cagggttggc cgtgctatgt ttcacaccag
     4201 atgttctctg tattgcagaa atgcctgacg tttaatccag ctaaaaggat atccgcctac
     4261 ggcgccctga atcacccgta cttccaagat ctggagagat acaaggacaa cctgaactct
     4321 cacctgccat ccaaccagag cacctcggag ctgaacacag ccggtttaaa ctctggttcc
     4381 gagggcagag gaagtctgtt aacatgcggt gacgtcgagg agaatcctgg cccatctggc
     4441 tccatgagca aaggagaaga acttttcact ggagttgtcc caattcttgt tgaattagat
     4501 ggtgatgtta atgggcacaa attttctgtc cgtggagagg gtgaaggtga tgctacaaac
     4561 ggaaaactca cccttaaatt tatttgcact actggaaaac tacctgttcc gtggccaaca
     4621 cttgtcacta ctctgaccta tggtgttcaa tgcttttccc gttatccgga tcacatgaaa
     4681 cggcatgact ttttcaagag tgccatgccc gaaggttatg tacaggaacg cactatatct
     4741 ttcaaagatg acgggaccta caagacgcgt gctgaagtca agtttgaagg tgataccctt
     4801 gttaatcgta tcgagttaaa gggtattgat tttaaagaag atggaaacat tcttggacac
     4861 aaactcgagt acaactttaa ctcacacaat gtatacatca cggcagacaa acaaaagaat
     4921 ggaatcaaag ctaacttcaa aattcgccac aacgttgaag atggttccgt tcaactagca
     4981 gaccattatc aacaaaatac tccaattggc gatggccctg tccttttacc agacaaccat
     5041 tacctgtcga cacaatctgt cctttcgaaa gatcccaacg aaaagcgtga ccacatggtc
     5101 cttcttgagt ttgtaactgc tgctgggatt acacatggca tggatgagct ctacaaaggt
     5161 tctgctggat ctgcagcagg atctggtatg agcaaaggag aagaactttt cactggagtt
     5221 gtcccaattc ttgttgaatt agatggtgat gttaatgggc acaaattttc tgtccgtgga
     5281 gagggtgaag gtgatgctac aaacggaaaa ctcaccctta aatttatttg cactactgga
     5341 aaactacctg ttccgtggcc aacacttgtc actactctga cctatggtgt tcaatgcttt
     5401 tcccgttatc cggatcacat gaaacggcat gactttttca agagtgccat gcccgaaggt
     5461 tatgtacagg aacgcactat atctttcaaa gatgacggga cctacaagac gcgtgctgaa
     5521 gtcaagtttg aaggtgatac ccttgttaat cgtatcgagt taaagggtat tgattttaaa
     5581 gaagatggaa acattcttgg acacaaactc gagtacaact ttaactcaca caatgtatac
     5641 atcacggcag acaaacaaaa gaatggaatc aaagctaact tcaaaattcg ccacaacgtt
     5701 gaagatggtt ccgttcaact agcagaccat tatcaacaaa atactccaat tggcgatggc
     5761 cctgtccttt taccagacaa ccattacctg tcgacacaat ctgtcctttc gaaagatccc
     5821 aacgaaaagc gtgaccacat ggtccttctt gagtttgtaa ctgctgctgg gattacacat
     5881 ggcatggatg agctctacaa atgaggcgcg ccggatccat tatgtacctg actgatgaag
     5941 ttcctatact ttctagagaa taggaacttc gaagggttcc gcaagctcta gtcgagcccc
     6001 agctggttct ttccgcctca gaagccatag agcccaccgc atccccagca tgcctgctat
     6061 tgtcttccca atcctccccc ttgctgtcct gccccacccc accccccaga atagaatgac
     6121 acctactcag acaatgcgat gcaatttcct cattttatta ggaaaggaca gtgggagtgg
     6181 caccttccag ggtcaaggaa ggcacggggg aggggcaaac aacagatggc tggcaactag
     6241 aaggcacagt cgaggctgat cagcgagctc tagagaattg atcccctcag aagaactcgt
     6301 caagaaggcg atagaaggcg atgcgctgcg aatcgggagc ggcgataccg taaagcacga
     6361 ggaagcggtc agcccattcg ccgccaagct cttcagcaat atcacgggta gccaacgcta
     6421 tgtcctgata gcggtccgcc acacccagcc ggccacagtc gatgaatcca gaaaagcggc
     6481 cattttccac catgatattc ggcaagcagg catcgccatg ggtcacgacg agatcatcgc
     6541 cgtcgggcat gcgcgccttg agcctggcga acagttcggc tggcgcgagc ccctgatgct
     6601 cttcgtccag atcatcctga tcgacaagac cggcttccat ccgagtacgt gctcgctcga
     6661 tgcgatgttt cgcttggtgg tcgaatgggc aggtagccgg atcaagcgta tgcagccgcc
     6721 gcattgcatc agccatgatg gatactttct cggcaggagc aaggtgagat gacaggagat
     6781 cctgccccgg cacttcgccc aatagcagcc agtcccttcc cgcttcagtg acaacgtcga
     6841 gcacagctgc gcaaggaacg cccgtcgtgg ccagccacga tagccgcgct gcctcgtcct
     6901 gcagttcatt cagggcaccg gacaggtcgg tcttgacaaa aagaaccggg cgcccctgcg
     6961 ctgacagccg gaacacggcg gcatcagagc agccgattgt ctgttgtgcc cagtcatagc
     7021 cgaatagcct ctccacccaa gcggccggag aacctgcgtg caatccatct tgttcaatgg
     7081 ccgatcccat ggtttagttc ctcaccttgt cgtattatac tatgccgata tactatgccg
     7141 atgattaatt gtcaacaggc tgcaggtcga aaggcccgga gatgaggaag aggagaacag
     7201 cgcggcagac gtgcgctttt gaagcgtgca gaatgccggg cctccggagg accttcgggc
     7261 gcccgccccg cccctgagcc cgcccctgag cccgcccccg gacccacccc ttcccagcct
     7321 ctgagcccag aaagcgaagg agcaaagctg ctattggccg ctgccccaaa ggcctacccg
     7381 cttccattgc tcagcggtgc tgtccatctg cacgagacta gtgagacgtg ctacttccat
     7441 ttgtcacgtc ctgcacgacg cgagctgcgg ggcggggggg aacttcctga ctaggggagg
     7501 agtagaaggt ggcgcgaagg ggccaccaaa gaacggagcc ggttggcgcc taccggtgga
     7561 tgtggaatgt gtgcgaggcc agaggccact tgtgtagcgc caagtgccca gcggggctgc
     7621 taaagcgcat gctccagact gccttgggaa aagcgcctcc cctacccggt agaatttcga
     7681 cgacctgcag ccaagctagc ttggctggac gtaaactcct cttcagacct gaagttccta
     7741 tactttctag agaataggaa cttcggaatt cgatatcaag cttatcgata ccttaattaa
     7801 ggttccacgg ggatgcccat gagctcgtca tctgaacaca ttggcggctg cgagtcccct
     7861 aagcaagcct ctcagagcag ttgaagattg ctggctgcca accttctggc tgccagcttc
     7921 tgggtgggct ctgccttacc aaggaaacca cctagtttac tgttcagaga tcaatgcaag
     7981 ggtgattgca gctttatgtt cgtttgtaca cttgtttgtt ttgtctgttt gtttcaagaa
     8041 cctggaaaac ttccagaaga agagaagctg ctgaccaatt gtgctgccat ttcgttttct
     8101 aaccttgaat gctgccagtg tagggtggga atccaggccc agctgagtta tgatgtaatc
     8161 cgcctgcagc tgctgggcct gctttggtac ttgtgagtgt gtgtgcatgc gtatgtgtgt
     8221 gtaagagaga agaggagggg agagaaagac ccctgatctc gtcaagtgtt actttttttt
     8281 tgtagaaaac aagaataatt gagttttaaa gagtagaggt gactgatagt aagaagggct
     8341 tgttcagtga aaggtgattc acaatggagt cttgttagga aggttggacc taagtcctca
     8401 gagttgcctt cctgtccaaa agcttttgct agcagtaaac aataaaggtt tagatgccac
     8461 aaaaaatggg gggaaccaca atatttttta agagactttt taaggcatac atcttctatt
     8521 tactctttgg aaagctgaac ttaatgtgtc ccaggcccta tatatagtac agtatgtact
     8581 taattgtttc tttggggaaa gatgctataa gtatcttatt acttgcaata catttaagga
     8641 gtgagtgtac ctcagatagg ttttaaagat agagagcacc tgttttctgg tgtgagatgt
     8701 tatcattttc ttcacgtctc ttgatacctt gataccttgt caccttaggg aatcacttcc
     8761 tgctctgact agaggcggga ataccatcta gctgtctcca ccacccacca tggcgcatct
     8821 gccttgtgct gccttgtgta gtgcgaagct ctcaaccacc agcacttcta attcattttc
     8881 ctgccactgc ctggctaacg acagatggcc cagctgcccc aatcccacac ccgcttgcac
     8941 gcttaccgtc tttcaccgaa tgctttgggc gtaggctccc attccgaaac cctaacagta
     9001 tccccttgtg cctttgtaat acagtcttcc ccctgccgca gctgaggtca cctaggcagt
     9061 gaagagtgct tgttctgtgt gtgtatagac tactaccgac tgtcacttgg tgtttcctat
     9121 ctttaagtgt atgttgtcag tgtaatgtct gaggaaatgt cttttcctct cttctagaga
     9181 taactactta ctctctaaag tgatctctct gtctgtccgc aggatgtgtt tctgggtttt
     9241 tgttcttttt tttttttttt tttttttttt gtctgaggcc tcatgtcagc cctgctttct
     9301 gcagagccag cctaccctgg gcattataaa caactgtacc ttactgtttt ctttcagtcc
     9361 tttacattct gtgccacctt tgccttatta tatccgtggc ttagtgctaa gctggcacta
     9421 ctccatagaa ggtgtagaag gcagctttcg ggaagcgtag ctgagttttg tttgaatcgt
     9481 gtttgagcac atttaagtaa acgctagatg atgcttcact gtagagcttt gaaagactgc
     9541 ctgatgtttc acacggcagt gtaggctcca gatggtgttt gtatgttatg gaccttactg
     9601 tgtttctcgt agctgaacaa tgcatgtggt tatttgtttg tttgtttttt ggtttctctg
     9661 tgtagccctg gctgtcctgg aactcactct gtagaccagg ctggcctcga attcagaaat
     9721 gcgcctgcct ctgcctccca agtgctggga tcaaaggcgt gcgccaccac gcccggcttt
     9781 gcatatggtt atctgtatgg agaagaatta gatgtctttc ctgagagctt tggaggttgg
     9841 gaatcatctt cttctgttta tttttatttt taacattaca ttacattctt ctgattcagt
     9901 atcgcaagaa aggatttttt cttttttcaa aaacaaaact ttaagaagcc agaggctagg
     9961 ctgagaatgt tctgcgctcg tcctcacagc tctgctgccg acactgccaa cgctcgagac
    10021 agtcagcctg gaacctctgg gacaaggtct actcgaggtc tcacttggac agcgagttct
    10081 gccttgtcat tgaacaaggt ccaaaataca gacatttttg ctagggccta gaaatcgacc
    10141 ataaaactca ctgcattgag gcctaatcca ttctatatta acatccatgt gataaagtga
    10201 aaataatttt gagggttttg ctttcgccaa aaaaaaaaaa gaaagaaaga aaaaaaaaga
    10261 aaggaagaaa aagaaaaaag aaaggattgt tttccttagc tttgctcaag aaaaatacag
    10321 ctatcctaaa ctggaatctt cccgtattct ttagagtctt aagtatagag tttaacttca
    10381 cttttgtcag ctgtcttgag cttatttcta agtagttttg ttttggcttt tggtgagtga
    10441 ggtagtggga ggttaatttt ggggtctttt ttttttttgg ctttgtttgt gtgtgtgtgt
    10501 gtgtgtgtgt gtttatgtgt ttggcttttg ggtttggttg gttagttgtt tagtttgttt
    10561 ttttgttgtt gtttaatttg ctttttctag gcagggtttc tctatgtggc cctggctgtc
    10621 ctagaacttg ctctgtagac caggctagcc tcaaactcag agatccactt gcctctgtct
    10681 cgagtgtgct tgaactaaag gcatgagcta ccctcctcag cccactccaa actttgagta
    10741 atatttttaa atcatagaca tgttggtatt agaaacatgg cgtattgtgg tggatggtag
    10801 agaaagggcg ccgcaacctc tcaggacatc atatgtctgg ccagctcttc attcatatag
    10861 gtgctctctg gatatgtctt tacatcagac tcacacctct gaaacccggt tttcacacaa
    10921 ggagacttgc cattggggtc tttagtagcc cagggaaaca tagagtaaac tgaagagctt
    10981 actgtgtgcc gcttatcctt agatggcctc atccagtcac aggtctttgt cagaatctaa
    11041 ccattgattc actgaggaga ccctactgcc ctgaggttcc actgaagcag cttttagtga
    11101 gactagggca agaatatacc gaatagcttc tgttcaaggt ttggttccag agactctacc
    11161 atttacagga ctggctggag ttacaacaac ctcttaaaga gggtagggcg ggttctccag
    11221 ttagaccccc taacctcaca ggtgagtagt cctaacctga cagccttaga aacacagttg
    11281 ccgagtacct gagagcaccc agtggcccgc cgcctcctat tcagcccctc tttaaagacg
    11341 acagacggtt aagccatgga gaagctgttc aggtagcacg gggccatttc tgcccccagc
    11401 ttgtcatgtt ttttctctca ggacccactc tctgaccagt agacttgaat ttagccccaa
    11461 gtgatttact aaactcagct cagaagctca cttaaaaata ggacctccac atggtcgtgc
    11521 taagtaaaaa tgaagacccc aagttgcaca gggtgctgct atctcaaaga caatactttc
    11581 ctttgaatag aaagctgtgc cgtccaccct gcaatgggtg ctagccaact tgcttgagaa
    11641 agtcaggagc aagcattttg agttttaaag aatgtcctga acttgtcttt taagtaaaca
    11701 tagctgcttt attaaaagct gtgactacta aagccagtca cagccatcgt cactgtggga
    11761 gttaaatcat gtcaactttc aaacatcaca gttgtttcaa tcttctgtcc aaagaactcc
    11821 tcttctggag aaaacctgat tttggttttg gctttttgtc ttgtcttgtt ttgtttttcc
    11881 tgagatgggg tctctctgtg aagccctggc tatcctggaa ctcagtgtgt agaccaggct
    11941 ggccttgaac tcatgtagat ctgcctgcta aaactaaaga catgcaccac catgacagct
    12001 gactattttt tatgatgagc acagaagtgg ctcacatttt cttaaagcac acttttgtca
    12061 catagtagag tctaggaaag aataacattg aaaatcccca tcccagtgtg acacttgtgt
    12121 aagcacatat ggatagccat tggaagaata catgcttgct tgtaacatga aattaaagac
    12181 attgtataat ttcttaatct ttaaaaatta ccaaaaaagt ggcaacagta tgtttaaggt
    12241 tattttaatt tttacataaa ttgttaacca tatttgtttg cctttaagta tattctaaga
    12301 attataatta agtataatta taagtcatcc cgcacattca gcacttatgg actaggtatg
    12361 tcaaaataca caaattctgt atttcttgtt tcataggaag aaagcagcca tctgaagctc
    12421 tactcatata tatgcctaag aatatctata caaaattaaa gtttaaaagg cctttgcctc
    12481 tcgtgcagct gagaatagac aacttataag ccttatccaa aaaccatgat aaagggatca
    12541 tgtgcatcct tacaaaaacc ccttatcctg tgcatactta catccaagta catcctaagg
    12601 aaggtgtccg tgcattcatc tgccacagtg gggaagctgc ctggtgcaag gtgtgagtcc
    12661 tggaatgatt agtgatagtg gtctcaaatg aggacacctt cgtctcccac cagtttcctt
    12721 cctatggaca ggctcagtcc agttatacag gccaggaggg gctggtgccc acagcctccc
    12781 gcctctgcaa ggtaggattg catatctcaa acatctctaa ctcagtttcg gctttgttgg
    12841 tcttgtttaa ggaaataagc cagtaagcca aaactatttt tgttgttgtt ctcttgtctt
    12901 ctgaaagaga ggggatatca attttaaatg tttctctcaa tttgatcttt caaatttttc
    12961 taataaagtg ccaaattgct gctcgtcttg tcattggcga attcgaacac gcagatgcag
    13021 tcggggcggc gcggtccgag gtccacttcg catattaagg tgacgcgtgt ggcctcgaac
    13081 accgagcgac cctgcagcga cccgcttaac agcgtcaaca gcgtgccgca gatcttggtg
    13141 gcgtgaaact cccgcacctc ttcggccagc gccttgtaga agcgcgtatg gcttcgtacc
    13201 ccggccatca acacgcgtct gcgttcgacc aggctgcgcg ttctcgcggc catagcaacc
    13261 gacgtacggc gttgcgccct cgccggcagc aagaagccac ggaagtccgc ccggagcaga
    13321 aaatgcccac gctactgcgg gtttatatag acggtcccca cgggatgggg aaaaccacca
    13381 ccacgcaact gctggtggcc ctgggttcgc gcgacgatat cgtctacgta cccgagccga
    13441 tgacttactg gcgggtgctg ggggcttccg agacaatcgc gaacatctac accacacaac
    13501 accgcctcga ccagggtgag atatcggccg gggacgcggc ggtggtaatg acaagcgccc
    13561 agataacaat gggcatgcct tatgccgtga ccgacgccgt tctggctcct catatcgggg
    13621 gggaggctgg gagctcacat gccccgcccc cggccctcac cctcatcttc gaccgccatc
    13681 ccatcgccgc cctcctgtgc tacccggccg cgcggtacct tatgggcagc atgacccccc
    13741 aggccgtgct ggcgttcgtg gccctcatcc cgccgacctt gcccggcacc aacatcgtgc
    13801 ttggggccct tccggaggac agacacatcg accgcctggc caaacgccag cgccccggcg
    13861 agcggctgga cctggctatg ctggctgcga ttcgccgcgt ttacgggcta cttgccaata
    13921 cggtgcggta tctgcagtgc ggcgggtcgt ggcgggagga ctggggacag ctttcgggga
    13981 cggccgtgcc gccccagggt gccgagcccc agagcaacgc gggcccacga ccccatatcg
    14041 gggacacgtt atttaccctg tttcgggccc ccgagttgct ggcccccaac ggcgacctgt
    14101 ataacgtgtt tgcctgggcc ttggacgtct tggccaaacg cctccgttcc atgcacgtct
    14161 ttatcctgga ttacgaccaa tcgcccgccg gctgccggga cgccctgctg caacttacct
    14221 ccgggatggt ccagacccac gtcaccaccc ccggctccat accgacgata tgcgacctgg
    14281 cgcgcacgtt tgcccgggag atgggggagg ctaactgaaa cacggaagga gacaataccg
    14341 gaaggaaccc gcgctatgac ggcaataaaa agacagaata aaacgcacgg gtgttgggtc
    14401 gtttgttcat aaacgcgggg ttcggtccca gggctggcac tctgtcgata ccccaccgag
    14461 accccattgg ggccaatacg cccgcgtttc ttccttttcc ccaccccacc ccccaagttc
    14521 gggtgaaggc ccagggctcg cagccaacgt cggggcggca ggccctgcca tagccactgg
    14581 ccccgtgggt tagggacggg gtcccccatg gggaatggtt tatggttcgt gggggttatt
    14641 attttgggcg ttgcgtgggg tcaggtccac gacccaagct tggctgcagg tcgagctcgc
    14701 gaaagcttgg cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
    14761 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc
    14821 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggcg cctgatgcgg
    14881 tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatggtgcac tctcagtaca
    14941 atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg
    15001 ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg
    15061 agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc
    15121 gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt
    15181 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
    15241 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
    15301 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
    15361 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
    15421 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
    15481 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
    15541 ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
    15601 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
    15661 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
    15721 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
    15781 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
    15841 acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
    15901 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
    15961 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
    16021 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
    16081 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
    16141 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
    16201 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
    16261 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccttaata
    16321 agatgatctt cttgagatcg ttttggtctg cgcgtaatct cttgctctga aaacgaaaaa
    16381 accgccttgc agggcggttt ttcgaaggtt ctctgagcta ccaactcttt gaaccgaggt
    16441 aactggcttg gaggagcgca gtcaccaaaa cttgtccttt cagtttagcc ttaaccggcg
    16501 catgacttca agactaactc ctctaaatca attaccagtg gctgctgcca gtggtgcttt
    16561 tgcatgtctt tccgggttgg actcaagacg atagttaccg gataaggcgc agcggtcgga
    16621 ctgaacgggg ggttcgtgca tacagtccag cttggagcga actgcctacc cggaactgag
    16681 tgtcaggcgt ggaatgagac aaacgcggcc ataacagcgg aatgacaccg gtaaaccgaa
    16741 aggcaggaac aggagagcgc acgagggagc cgccaggggg aaacgcctgg tatctttata
    16801 gtcctgtcgg gtttcgccac cactgatttg agcgtcagat ttcgtgatgc ttgtcagggg
    16861 ggcggagcct atggaaaaac ggctttgccg cggccctctc acttccctgt taagtatctt
    16921 cctggcatct tccaggaaat ctccgccccg ttc
//
