LOCUS       sequence-270229-         670 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Used to sense miRNA activity using fluorescence based tools. 
            hsa-miR-181b-2-3p target in 3' UTR of of mKate. miRNA activity can 
            be measured by the repression of mKate2 relative to EBFP2 
            fluorescent proteins . Plasmid is inherently unstable; please see 
            Depositor Comments.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 670)
  AUTHORS   Gam JJ, Babb J, Weiss R
  TITLE     A mixed antagonistic/synergistic miRNA repression model enables 
            accurate predictions of multi-input miRNA sensor activity.
  JOURNAL   Nat Commun. 2018 Jun 22;9(1):2430. doi: 10.1038/s41467-018-04575-0.
  PUBMED    29934631
REFERENCE   2  (bases 1 to 670)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-06-22"; volume: "9(1):2430. doi"; pages: " 
            10.1038/s41467-018-04575-0"
COMMENT     Sequence Label: LSB-hsa-miR-181b-2-3p
FEATURES             Location/Qualifiers
     source          1..670
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(112..136)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(352..371)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    417..472
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(471..490)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
ORIGIN
        1 gaatcaagga ggccgacaaa gagacatacg tcgagcagca cgaggtggct gtggccagat
       61 actgcgacct ccctagcaaa ctggggcaca aacttaattg agaattctac cacccagctt
      121 tcttgtacaa agtggtacgt ctcaagacca gaggtctctg cttcccagcg atgcattcat
      181 tgatcagtga gtgcattcat tgatcagtga gtgcattcat tgatcagtga gtgcattcat
      241 tgatcagtga gcaaccagaa gagacctgtg cttcactcct caggtgcagg ctgcctatca
      301 gaaggtggtg gctggtgtgg ccaatgccct ggctcacaaa taccactgag atctttttcc
      361 ctctgccaaa aattatgggg acatcatgaa gccccttgag catctgactt ctggctaata
      421 aaggaaattt attttcattg caatagtgtg ttggaatttt ttgtgtctct cactcggaag
      481 gacatatggg agggcaaatc atttaaaaca tcagaatgag tatttggttt agagtttggc
      541 aacatatgcc catatgctgg ctgccatgaa caaaggttgg ctataaagag gtcatcagta
      601 tatgaaacag ccccctgctg tccattcctt attccataga aaagccttga cttgaggtta
      661 gatttttttt
//
