LOCUS       sequence-27025-e         713 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 713)
  AUTHORS   Kita-Matsuo H, Barcova M, Prigozhina N, Salomonis N, Wei K, Jacot 
            JG, Nelson B, Spiering S, Haverslag R, Kim C, Talantova M, Bajpai R,
            Calzolari D, Terskikh A, McCulloch AD, Price JH, Conklin BR, Chen 
            HS, Mercola M
  TITLE     Lentiviral vectors and protocols for creation of stable hESC lines 
            for fluorescent tracking and drug resistance selection of 
            cardiomyocytes.
  JOURNAL   PLoS ONE. 2009  . 4(4):e5046.
  PUBMED    19352491
REFERENCE   2  (bases 1 to 713)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE. 
            2009  . 4(4):e5046."
COMMENT     Sequence Label: aMHC-puro rex-neo
FEATURES             Location/Qualifiers
     source          1..713
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     LTR             16..196
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    243..367
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    417..534
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     609..625
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     609..625
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 gctggtttag tgaccgggtc tctctggtta gaccagattt gagcctggga gctctctggc
       61 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
      121 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
      181 tggaaaatct ctagcagtgg cgcccgaaca gggacctgaa agcgaaaggg aaaccagagg
      241 agctctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc
      301 gactgcagag tacgccaaaa ttttgactag cggaggctag aaggagagag atgggtgcga
      361 gagcgtcagt attaagcggg ggaaaataag cccgggatgg cagtattcat ccacaatttt
      421 aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca
      481 acagacatac aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt
      541 tattacaggg acagcggcct atggaatgaa agaccccacc tgtcgagctc caccgcggtg
      601 gcggccgctc tagaactagt ggatcctgca aggtcacaca agggtctcca cccaccaggt
      661 gccctagtct caatttcagt ttccatgccc ttgtctcaca tgctgggcct ccc
//
