LOCUS       sequence-270307-         661 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Used to sense miRNA activity using fluorescence based tools. 
            hsa-miR-186-5p target in 3' UTR of of mKate. miRNA activity can be 
            measured by the repression of mKate2 relative to EBFP2 fluorescent 
            proteins . Plasmid is inherently unstable; please see Depositor 
            Comments.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 661)
  AUTHORS   Gam JJ, Babb J, Weiss R
  TITLE     A mixed antagonistic/synergistic miRNA repression model enables 
            accurate predictions of multi-input miRNA sensor activity.
  JOURNAL   Nat Commun. 2018 Jun 22;9(1):2430. doi: 10.1038/s41467-018-04575-0.
  PUBMED    29934631
REFERENCE   2  (bases 1 to 661)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-06-22"; volume: "9(1):2430. doi"; pages: " 
            10.1038/s41467-018-04575-0"
COMMENT     Sequence Label: LSB-hsa-miR-186-5p
FEATURES             Location/Qualifiers
     source          1..661
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(111..135)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(359..378)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    424..479
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(478..497)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
ORIGIN
        1 aatcaaggag gccgacaaag agacatacgt cgagcagcac gaggtggctg tggccagata
       61 ctgcgacctc cctagcaaac tggggcacaa acttaattga gaattctacc acccagcttt
      121 cttgtacaaa gtggtacgtc tcaagaccag aggtctctgc ttgaacacgg agcccaaaag
      181 gagaattctt tgagcccaaa aggagaattc tttgagccca aaaggagaat tctttgagcc
      241 caaaaggaga attctttgca accagaagag acctgtgctt cactcctcag gtgcaggctg
      301 cctatcagaa ggtggtggct ggtgtggcca atgccctggc tcacaaatac cactgagatc
      361 tttttccctc tgccaaaaat tatggggaca tcatgaagcc ccttgagcat ctgacttctg
      421 gctaataaag gaaatttatt ttcattgcaa tagtgtgttg gaattttttg tgtctctcac
      481 tcggaaggac atatgggagg gcaaatcatt taaaacatca gaatgagtat ttggtttaga
      541 gtttggcaac atatgcccat atgctggctg ccatgaacaa aggttggcta taaagaggtc
      601 atcagtatat gaaacagccc cctgctgtcc attccttatt ccatagaaaa gccttgactt
      661 g
//
