LOCUS       sequence-270333-        7995 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Exchange cassette containing inducible TauWT 2N4R-EGFP fusion, 
            CAG-driven rtTA3, Puromycin resistance marker, targets AAVS1 site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7995)
  AUTHORS   Wang X, Friesen E, Muller I, Lemieux M, Dukart R, Maia IB, Kalia S, 
            Schmitt-Ulms G
  TITLE     Rapid Generation of Human Neuronal Cell Models Enabling Inducible 
            Expression of Proteins-of-interest for Functional Studies.
  JOURNAL   Bio Protoc. 2020 May 5;10(9):e3615. doi: 10.21769/BioProtoc.3615. 
            eCollection 2020 May 5.
  PUBMED    33659578
REFERENCE   2  (bases 1 to 7995)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Bio 
            Protoc."; date: "2020-05-5"; volume: "10(9):e3615. doi"; pages: " 
            10.21769/BioProtoc.3615. eCollection 2020 May 5"
COMMENT     Sequence Label: AAVS1 CAG rtTA3 TauWT 2N4R-EGFP
FEATURES             Location/Qualifiers
     source          1..7995
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      23..611
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     512..531
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     765..782
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    complement(990..1023)
                     /label=lox66
                     /bound_moiety="Cre recombinase"
                     /note="Right element (RE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    1026..1059
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     misc_feature    1064..1089
                     /label=SA
                     /note="splice acceptor site"
     CDS             1113..1166
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             1176..1775
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(1176..1195)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     1672..1692
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     protein_bind    1778..1811
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     polyA_signal    1814..2038
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     polyA_signal    2045..2126
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(2264..2971)
                     /codon_start=1
                     /product="reverse tetracycline transactivator 3"
                     /label=rtTA3
                     /note="activates expression from Tet-responsive promoters 
                     in the presence of doxycycline"
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPG"
     primer_bind     complement(3015..3034)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     complement(3096..3118)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     polyA_signal    3886..4007
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3897..3916)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(join(4130..4843,4844..4846,4847..4849))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(4133..4843,4844..4846,4847..4849))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4175..4196)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     4524..4543
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4783..4804
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      4846..4855
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      6223..6232
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(6264..6288)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(6305..6619)
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     primer_bind     complement(6308..6332)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    6379..6397
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6415..6433
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6450..6468
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6486..6504
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6522..6540
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6557..6575
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6593..6611
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    complement(6636..6669)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     CDS             complement(join(7034..7825,7826..7894))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7657..7676
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
       61 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
      121 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
      181 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
      241 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
      301 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
      361 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
      421 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
      481 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
      541 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
      601 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
      661 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
      721 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
      781 aggaactgtc atggcatctt ccaggggtcc gagagctcag ctagtcttct tcctccaacc
      841 cgggccccta tgtccacttc aggacagcat gtttgctgcc tccagggatc ctgtgtcccc
      901 gagctgggac caccttatat tcccagggcc ggttaatgtg gctctggttc tgggtacttt
      961 tatctgtccc ctccacccca cagtggggca taacttcgta tagcatacat tatacgaacg
     1021 gtaaagaagt tcctattctc tagaaagtat aggaacttcg cttctgacct cttctcttcc
     1081 tcccacaggg cctcgagaga tctggcagcg gagagggcag aggaagtctt ctaacatgcg
     1141 gtgacgtgga ggagaatccc ggccctaggc tcgagatgac cgagtacaag cccacggtgc
     1201 gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg
     1261 actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg gtcaccgagc
     1321 tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg
     1381 acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg
     1441 ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga
     1501 tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg
     1561 gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg
     1621 aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc
     1681 ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc
     1741 gcacctggtg catgacccgc aagcccggtg cctgatcgaa gttcctattc tctagaaagt
     1801 ataggaactt cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg
     1861 ccttccttga ccctggaaga tgccactccc actgtccttt cctaataaaa tgaggaaatt
     1921 gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc
     1981 aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg ctctatgggt
     2041 cgaggcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa
     2101 ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg
     2161 ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa cctctacaaa tgtggtatgg
     2221 ctgattatga tcctctagag atatcgtcga caagcttatc gatttacccg gggagcatgt
     2281 caaggtcaaa atcgtcaagg gcatcggctg ggagcatgtc taagtcaaaa tcgtcaaggg
     2341 cgtcggtcgg cccgccgctt tcgcacttta gctgtttctc caggccacat atgattagtt
     2401 ccaggccgaa aaggaaggca ggttcggctc cctgccggtc gaacagctca attgcttgtc
     2461 tcagaagtgg gggcatagaa tcggtggtag gtgtctctct ttcctctttt gctacttgat
     2521 gctcctgttc ctccaatacg cagcccagtg taaagtggcc cacggcggac agagcgtaca
     2581 gtgcgttctc cagggagaag ccttgctgac acaggaacgc gagctgattt tccagggttt
     2641 cgtactgttt ctctgttggg cgggtgccga gatgcacttt agccccgtcg cgatgtgaga
     2701 ggagagcaca gcggtatgac ttggcgttgt tccgcagaaa gtcttgccat gactcgcctt
     2761 ccagggggca gaagtgggta tgatgcctgt ccagcatctc gattggcagg gcatcgagca
     2821 gggcccgctt gttcttcacg tgccagtaca gggtaggctg ctcaactccc agcttttgag
     2881 cgagtttcct tgtcgtcagg ccttcgatac cgacaccatt gagtaattcc agagctccgt
     2941 ttatgacttt gctcttgtcc agcctagaca tggtggcact agtgatccaa ttctttgcca
     3001 aaatgatgag acagcacaat aaccagcacg ttgcccagga gctgtaggaa aaagaagaag
     3061 gcatgaacat ggttagcaga ggctctagag ccgccggtca cacgccagaa gccgaacccc
     3121 gccctgcccc gtcccccccg aaggcagccg tccccccgcg gacagccccg agccgctccc
     3181 cccgcacaaa gggccctccc ggagcccctc aaggctttca cgcagccaca gaaaagaaac
     3241 aagccgtcat taaaccaagc gctaattaca gcccggagga gaagggccgt cccgcccgct
     3301 cacctgtggg agtaacgcgg tcagtcagag ccggggcggg cggcgcgagg cggcggcgga
     3361 gcggggcacg gggcgaaggc agcgcgcagc gactcccgcc cgccgcgcgc ttcgcttttt
     3421 atagggccgc cgccgccgcc gcctcgccat aaaaggaaac tttcggagcg cgccgctctg
     3481 attggctgcc gccgcacctc tccgcctcgc cccgccccca tcgctgcaca aaataattaa
     3541 aaaataaata aatacaaaat tgggggtggg gagggggggg agatggggag agtgaagcag
     3601 aacgtggggc tcacctcgac catggtaata gcgatgacta atacgtagat gtactgccaa
     3661 gtaggaaagt cccataaggt catgtactgg gcataatgcc aggcgggcca tttaccgtca
     3721 ttgacgtcaa tagggggcgt acttggcata tgatacactt gatgtactgc caagtgggca
     3781 gtttaccgta aatactccac ccattgacgt caatggaaag tccctattgg cgttactatg
     3841 ggaacatacg tcattattga cgtcaatggt acgatcttcc tagcttaaga tacattgatg
     3901 agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg
     3961 atgctattgc tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt
     4021 gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttaa agcaagtaaa
     4081 acctctacaa atgtggtatg gctgattatg atctagagtc gcggccgctt tacttgtaca
     4141 gctcgtccat gccgagagtg atcccggcgg cggtcacgaa ctccagcagg accatgtgat
     4201 cgcgcttctc gttggggtct ttgctcaggg cggactgggt gctcaggtag tggttgtcgg
     4261 gcagcagcac ggggccgtcg ccgatggggg tgttctgctg gtagtggtcg gcgagctgca
     4321 cgctgccgtc ctcgatgttg tggcggatct tgaagttcac cttgatgccg ttcttctgct
     4381 tgtcggccat gatatagacg ttgtggctgt tgtagttgta ctccagcttg tgccccagga
     4441 tgttgccgtc ctccttgaag tcgatgccct tcagctcgat gcggttcacc agggtgtcgc
     4501 cctcgaactt cacctcggcg cgggtcttgt agttgccgtc gtccttgaag aagatggtgc
     4561 gctcctggac gtagccttcg ggcatggcgg acttgaagaa gtcgtgctgc ttcatgtggt
     4621 cggggtagcg gctgaagcac tgcacgccgt aggtcagggt ggtcacgagg gtgggccagg
     4681 gcacgggcag cttgccggtg gtgcagatga acttcagggt cagcttgccg taggtggcat
     4741 cgccctcgcc ctcgccggac acgctgaact tgtggccgtt tacgtcgccg tccagctcga
     4801 ccaggatggg caccaccccg gtgaacagct cctcgccctt gctcaccatg gtggcgaccg
     4861 gtggatcccg ggcccgcggt accgtcgact gcagaattct tatcaaaccc tgcttggcca
     4921 gggaggcaga cacctcgtca gctagcgtgg cgagctgggg cgagtctacc atgtcgatgc
     4981 tgccggtgga ggagacattg ctgagatgcc gtggagacgt gtccccagac accactggcg
     5041 acttgtacac gatctccgcc ccgtggtctg tcttggcttt ggcgttctcg cggaaggtca
     5101 gcttgtgggt ttcaatcttt ttatttcctc cgccagggac gtgggtgata ttgtccaggg
     5161 acccaatctt cgactggact ctgtccttga agtcaagctt ctcagatttt acttccacct
     5221 ggccacctcc tggtttatga tggatgttgc ctaatgagcc acacttggag gtcaccttgc
     5281 tcaggtcaac tggtttgtag actatttgca cactgccgcc tcccgggacg tgtttgatat
     5341 tatcctttga gccacacttg gactggacgt tgctaagatc cagcttctta ttaattatct
     5401 gcaccttccc gcctcccggc tggtgcttca ggttctcagt ggagccgatc ttggacttga
     5461 cattcttcag gtctggcatg ggcacggggg ctgtctgcag gcggctcttg gcggaagacg
     5521 gcgacttggg tggagtacgg accactgcca ccttcttggg ctcccgggtg ggtggggttg
     5581 gaagggacgg ggtgcgggag cggctgccgg gagtgcctgg ggagccgggg ctgctgtagc
     5641 cgctgcgatc ccctgatttt ggaggttcac cagagctggg tggtgtcttt ggagcgggcg
     5701 gggtttttgc tggaatcctg gtggcgttgg cctggccctt ctggcctgga ggggctgctc
     5761 cccgcggtgt ggcgatcttc gttttaccat cagccccctt ggcttttttg tcatcgcttc
     5821 cagtcccgtc tttgctttta ctgaccatgc gagcttgggt cacgtgacca gcagcttcgt
     5881 cttccaggct gggggtgtct ccaatgcctg cttcttcagc tgtggttcct tctgggatct
     5941 ccgtgtgggg ctgcgcggca gcctgcttgc cgggagctcc ctcatccact aagggtgctg
     6001 tcacatcttc cgctgttgga gtgctcttag catcagaggt ttcagagccc ggttcctcag
     6061 atccgtcctc agtgggggtc tgcaggggag attctttcag gccagcgtcc gtgtcaccct
     6121 cttggtcttg gtgcatggtg tagcccccct gatctttcct gtcccccaac ccgtacgtcc
     6181 cagcgtgatc ttccatcact tcgaactcct ggcggggctc agccatggtg gcgctgagga
     6241 ccggtccaac ctgaaaaccg cggagcctgc ttttttgtac aaacttgttg atcggaattc
     6301 tccaggcgat ctgacggttc actaaacgag ctctgcttat ataggcctcc caccgtacac
     6361 gcctacctcg acatacgttc tctatcactg atagggagta aactcgacat acgttctcta
     6421 tcactgatag ggataaactc gacatacgtt ctctatcact gatagggagt aaactcgaca
     6481 tacgttctct atcactgata gggagtaaac tcgacatacg ttctctatca ctgataggga
     6541 gtaaactcga catcgttctc tatcactgat agggagtaaa ctcgacatac gttctctatc
     6601 actgataggg agtaaactcg acagtactaa gctttataac ttcgtatagg atactttata
     6661 cgaagttata ctagggacag gattggtgac agaaaagccc catccttagg cctcctcctt
     6721 cctagtctcc tgatattggg tctaaccccc acctcctgtt aggcagattc cttatctggt
     6781 gacacacccc catttcctgg agccatctct ctccttgcca gaacctctaa ggtttgctta
     6841 cgatggagcc agcatcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     6901 tatttttcta aatacattca aatatgtatc cgctcatgag attatcaaaa aggatcttca
     6961 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     7021 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     7081 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     7141 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     7201 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     7261 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     7321 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     7381 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     7441 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     7501 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     7561 taagatgctt ttctgtgact ggtgaatact caaccaagtc attctgagaa tagtgtatgc
     7621 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     7681 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     7741 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     7801 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     7861 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     7921 gcatttatca gggttattgt ctcatgacca aaatccctta acgtgagttt tcgttccact
     7981 gagcgtcaga ccccg
//
