LOCUS       sequence-270342-        1419 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses N-teminal Flag tagged   aa 721-1176 of LARS1 .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1419)
  AUTHORS   Yoon MS, Son K, Arauz E, Han JM, Kim S, Chen J
  TITLE     Leucyl-tRNA Synthetase Activates Vps34 in Amino Acid-Sensing mTORC1 
            Signaling.
  JOURNAL   Cell Rep. 2016 Aug 9;16(6):1510-1517. doi: 
            10.1016/j.celrep.2016.07.008. Epub 2016 Jul 28.
  PUBMED    27477288
REFERENCE   2  (bases 1 to 1419)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2016.07.008"; journalName: "Cell Rep"; date: 
            "2016-08-9- 9"; volume: "16"; issue: "6"; pages: "1510-1517"
COMMENT     Sequence Label: pcDNA8-Flag-LARS(721-1176aa)
FEATURES             Location/Qualifiers
     source          1..1419
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     62..81
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        62..80
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      102..111
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ccngggctnt agcgagctct ctggctacta gagaacccac tgcttactgg cttatcgaaa
       61 ttaatacgac tcactatagg gagacccaag cttggggggc ggccgccatg gattataaag
      121 atgatgatga tggatcggat cccatgacag gcaacttcct cactttgacc caagctattg
      181 acaaattttc agcagatgga atgcgtttgg ctctggctga tgctggtgac actgtagaag
      241 atgccaactt tgtggaagcc atggcagatg caggtattct ccgtctgtac acctgggtag
      301 agtgggtgaa agaaatggtt gccaactggg acagcctaag aagtggtcct gccagcactt
      361 tcaatgatag agtttttgcc agtgaattga atgcaggaat tataaaaaca gatcaaaact
      421 atgaaaagat gatgtttaaa gaagctttga aaacagggtt ttttgagttt caggccgcaa
      481 aagataagta ccgtgaattg gctgtggaag ggatgcacag agaacttgtg ttccggttta
      541 ttgaagttca gacacttctc ctcgctccat tctgtccaca tttgtgtgag cacatctgga
      601 cactcctggg aaagcctgac tcaattatga atgcttcatg gcctgtggca ggtcctgtta
      661 atgaagtttt aatacactcc tcacagtatc ttatggaagt aacacatgac cttagactac
      721 gactcaagaa ctatatgatg ccagctaaag ggaagaagac tgacaaacaa cccctgcaga
      781 agccctcaca ttgcaccatc tatgtggcaa agaactatcc accttggcaa cataccaccc
      841 tgtctgttct acgtaaacac ttttgaaggn ccaatancgg aaaaactgcc tgacaacaaa
      901 gtcattgcta gtgaactagg cngtatgccn gaaactgaag aaaatacatg aagaaaggtc
      961 ntgccntttt gttgccctga ttaaggnaaa tcngggnnna agatggggcc nccntatttt
     1021 tggatttgca attagnantt ngatgaaaag ggctntgcct tatggaanaa nanagntcta
     1081 tctgactaaa ttcccttgaa ccaaaaaanc ataaaaattn caattttgcc cncccaancc
     1141 aaaaaaaaaa tncggggaaa cngntgtccc tggnaaaccc cttaantttt taaaaaaaaa
     1201 cccggnggtt ccttttttcn gggaancccc ncccnnccag gnccctttcc accaaanttg
     1261 aaccggnnag ggaanatcgn tntnccaaan cggngtttaa gaaaaaancc ggaaattaaa
     1321 cccttcnaag aaanntggna attttntncc nggnggcccc cccaatttcn tngggaaaaa
     1381 aancccnaaa cccccttttg gngggntttt taggcacca
//
