LOCUS       sequence-270351-       21252 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  T-DNA encoding Fungal Bioluminescent Pathway Components, complete 
            pathway, without recycling gene CPH.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 21252)
  AUTHORS   Khakhar A, Starker CG, Chamness JC, Lee N, Stokke S, Wang C, Swanson
            R, Rizvi F, Imaizumi T, Voytas DF
  TITLE     Building customizable auto-luminescent luciferase-based reporters in
            plants.
  JOURNAL   Elife. 2020 Mar 25;9. pii: 52786. doi: 10.7554/eLife.52786.
  PUBMED    32209230
REFERENCE   2  (bases 1 to 21252)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2020-03-25"; volume: "9. pii: 52786. doi"; pages: " 
            10.7554/eLife.52786"
COMMENT     Sequence Label: P303-FBP_2
FEATURES             Location/Qualifiers
     source          1..21252
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(5..22)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5..21)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(14..36)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    224..248
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             1549..2178
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             2612..3679
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      3745..3939
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(4175..4197)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    4283..4423
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     4338..4357
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(4438..4455)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4609..5197)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4689..4708)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(5284..6078)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    6503..6527
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    6605..6779
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(6836..7633)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLL
                     SSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEH
                     QGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDI
                     ALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6947..6966)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     7557..7576
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(7696..8372)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     protein_bind    8563..8584
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8599..8604,8605..8622,8623..8629)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8637..8653
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8642..8664
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8661..8677
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8661..8677
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        8805..9150
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     terminator      10301..10501
                     /label=gene 7 terminator
                     /note="transcription terminator for octopine-type Ti 
                     plasmid gene 7"
     terminator      12262..12969
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     terminator      18561..18809
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     promoter        19654..19700
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     terminator      20524..21231
                     /label=OCS terminator
                     /note="octopine synthase terminator"
ORIGIN
        1 tggcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta
       61 atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg
      121 atcgcccttc ccaacagttg cgcagcctga atggcgaatg ctagagcagc ttgagcttgg
      181 atcagattgt cgtttcccgc cttcagttta aactatcagt gtttgacagg atatattggc
      241 gggtaaacct aagagaaaag agcgtttatt agaataatcg gatatttaaa agggcgtgaa
      301 aaggtttatc cgttcgtcca tttgtatgtg catgccaacc acagggttcc cctcgggatc
      361 aaagtacttt gatccaaccc ctccgctgct atagtgcagt cggcttctga cgttcagtgc
      421 agccgtcttc tgaaaacgac atgtcgcaca agtcctaagt tacgcgacag gctgccgccc
      481 tgcccttttc ctggcgtttt cttgtcgcgt gttttagtcg cataaagtag aatacttgcg
      541 actagaaccg gagacattac gccatgaaca agagcgccgc cgctggcctg ctgggctatg
      601 cccgcgtcag caccgacgac caggacttga ccaaccaacg ggccgaactg cacgcggccg
      661 gctgcaccaa gctgttttcc gagaagatca ccggcaccag gcgcgaccgc ccggagctgg
      721 ccaggatgct tgaccaccta cgccctggcg acgttgtgac agtgaccagg ctagaccgcc
      781 tggcccgcag cacccgcgac ctactggaca ttgccgagcg catccaggag gccggcgcgg
      841 gcctgcgtag cctggcagag ccgtgggccg acaccaccac gccggccggc cgcatggtgt
      901 tgaccgtgtt cgccggcatt gccgagttcg agcgttccct aatcatcgac cgcacccgga
      961 gcgggcgcga ggccgccaag gcccgaggcg tgaagtttgg cccccgccct accctcaccc
     1021 cggcacagat cgcgcacgcc cgcgagctga tcgaccagga aggccgcacc gtgaaagagg
     1081 cggctgcact gcttggcgtg catcgctcga ccctgtaccg cgcacttgag cgcagcgagg
     1141 aagtgacgcc caccgaggcc aggcggcgcg gtgccttccg tgaggacgca ttgaccgagg
     1201 ccgacgccct ggcggccgcc gagaatgaac gccaagagga acaagcatga aaccgcacca
     1261 ggacggccag gacgaaccgt ttttcattac cgaagagatc gaggcggaga tgatcgcggc
     1321 cgggtacgtg ttcgagccgc ccgcgcacgt ctcaaccgtg cggctgcatg aaatcctggc
     1381 cggtttgtct gatgccaagc tggcggcctg gccggccagc ttggccgctg aagaaaccga
     1441 gcgccgccgt ctaaaaaggt gatgtgtatt tgagtaaaac agcttgcgtc atgcggtcgc
     1501 tgcgtatatg atgcgatgag taaataaaca aatacgcaag gggaacgcat gaaggttatc
     1561 gctgtactta accagaaagg cgggtcaggc aagacgacca tcgcaaccca tctagcccgc
     1621 gccctgcaac tcgccggggc cgatgttctg ttagtcgatt ccgatcccca gggcagtgcc
     1681 cgcgattggg cggccgtgcg ggaagatcaa ccgctaaccg ttgtcggcat cgaccgcccg
     1741 acgattgacc gcgacgtgaa ggccatcggc cggcgcgact tcgtagtgat cgacggagcg
     1801 ccccaggcgg cggacttggc tgtgtccgcg atcaaggcag ccgacttcgt gctgattccg
     1861 gtgcagccaa gcccttacga catatgggcc accgccgacc tggtggagct ggttaagcag
     1921 cgcattgagg tcacggatgg aaggctacaa gcggcctttg tcgtgtcgcg ggcgatcaaa
     1981 ggcacgcgca tcggcggtga ggttgccgag gcgctggccg ggtacgagct gcccattctt
     2041 gagtcccgta tcacgcagcg cgtgagctac ccaggcactg ccgccgccgg cacaaccgtt
     2101 cttgaatcag aacccgaggg cgacgctgcc cgcgaggtcc aggcgctggc cgctgaaatt
     2161 aaatcaaaac tcatttgagt taatgaggta aagagaaaat gagcaaaagc acaaacacgc
     2221 taagtgccgg ccgtccgagc gcacgcagca gcaaggctgc aacgttggcc agcctggcag
     2281 acacgccagc catgaagcgg gtcaactttc agttgccggc ggaggatcac accaagctga
     2341 agatgtacgc ggtacgccaa ggcaagacca ttaccgagct gctatctgaa tacatcgcgc
     2401 agctaccaga gtaaatgagc aaatgaataa atgagtagat gaattttagc ggctaaagga
     2461 ggcggcatgg aaaatcaaga acaaccaggc accgacgccg tggaatgccc catgtgtgga
     2521 ggaacgggcg gttggccagg cgtaagcggc tgggttgtct gccggccctg caatggcact
     2581 ggaaccccca agcccgagga atcggcgtga cggtcgcaaa ccatccggcc cggtacaaat
     2641 cggcgcggcg ctgggtgatg acctggtgga gaagttgaag gccgcgcagg ccgcccagcg
     2701 gcaacgcatc gaggcagaag cacgccccgg tgaatcgtgg caagcggccg ctgatcgaat
     2761 ccgcaaagaa tcccggcaac cgccggcagc cggtgcgccg tcgattagga agccgcccaa
     2821 gggcgacgag caaccagatt ttttcgttcc gatgctctat gacgtgggca cccgcgatag
     2881 tcgcagcatc atggacgtgg ccgttttccg tctgtcgaag cgtgaccgac gagctggcga
     2941 ggtgatccgc tacgagcttc cagacgggca cgtagaggtt tccgcagggc cggccggcat
     3001 ggccagtgtg tgggattacg acctggtact gatggcggtt tcccatctaa ccgaatccat
     3061 gaaccgatac cgggaaggga agggagacaa gcccggccgc gtgttccgtc cacacgttgc
     3121 ggacgtactc aagttctgcc ggcgagccga tggcggaaag cagaaagacg acctggtaga
     3181 aacctgcatt cggttaaaca ccacgcacgt tgccatgcag cgtacgaaga aggccaagaa
     3241 cggccgcctg gtgacggtat ccgagggtga agccttgatt agccgctaca agatcgtaaa
     3301 gagcgaaacc gggcggccgg agtacatcga gatcgagcta gctgattgga tgtaccgcga
     3361 gatcacagaa ggcaagaacc cggacgtgct gacggttcac cccgattact ttttgatcga
     3421 tcccggcatc ggccgttttc tctaccgcct ggcacgccgc gccgcaggca aggcagaagc
     3481 cagatggttg ttcaagacga tctacgaacg cagtggcagc gccggagagt tcaagaagtt
     3541 ctgtttcacc gtgcgcaagc tgatcgggtc aaatgacctg ccggagtacg atttgaagga
     3601 ggaggcgggg caggctggcc cgatcctagt catgcgctac cgcaacctga tcgagggcga
     3661 agcatccgcc ggttcctaat gtacggagca gatgctaggg caaattgccc tagcagggga
     3721 aaaaggtcga aaaggtctct ttcctgtgga tagcacgtac attgggaacc caaagccgta
     3781 cattgggaac cggaacccgt acattgggaa cccaaagccg tacattggga accggtcaca
     3841 catgtaagtg actgatataa aagagaaaaa aggcgatttt tccgcctaaa actctttaaa
     3901 acttattaaa actcttaaaa cccgcctggc ctgtgcataa ctgtctggcc agcgcacagc
     3961 cgaagagctg caaaaagcgc ctacccttcg gtcgctgcgc tccctacgcc ccgccgcttc
     4021 gcgtcggcct atcgcggccg ctggccgctc aaaaatggct ggcctacggc caggcaatct
     4081 accagggcgc ggacaagccg cgccgtcgcc actcgaccgc cggcgcccac atcaaggcac
     4141 cctgcctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga
     4201 cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag
     4261 cgggtgttgg cgggtgtcgg ggcgcagcca tgacccagtc acgtagcgat agcggagtgt
     4321 atactggctt aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg
     4381 tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgctctt ccgcttcctc
     4441 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     4501 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     4561 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     4621 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     4681 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     4741 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     4801 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     4861 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     4921 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     4981 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     5041 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     5101 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     5161 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     5221 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgcattctag
     5281 gtactaaaac aattcatcca gtaaaatata atattttatt ttctcccaat caggcttgat
     5341 ccccagtaag tcaaaaaata gctcgacata ctgttcttcc ccgatatcct ccctgatcga
     5401 ccggacgcag aaggcaatgt cataccactt gtccgccctg ccgcttctcc caagatcaat
     5461 aaagccactt actttgccat ctttcacaaa gatgttgctg tctcccaggt cgccgtggga
     5521 aaagacaagt tcctcttcgg gcttttccgt ctttaaaaaa tcatacagct cgcgcggatc
     5581 tttaaatgga gtgtcttctt cccagttttc gcaatccaca tcggccagat cgttattcag
     5641 taagtaatcc aattcggcta agcggctgtc taagctattc gtatagggac aatccgatat
     5701 gtcgatggag tgaaagagcc tgatgcactc cgcatacagc tcgataatct tttcagggct
     5761 ttgttcatct tcatactctt ccgagcaaag gacgccatcg gcctcactca tgagcagatt
     5821 gctccagcca tcatgccgtt caaagtgcag gacctttgga acaggcagct ttccttccag
     5881 ccatagcatc atgtcctttt cccgttccac atcataggtg gtccctttat accggctgtc
     5941 cgtcattttt aaatataggt tttcattttc tcccaccagc ttatatacct tagcaggaga
     6001 cattccttcc gtatctttta cgcagcggta tttttcgatc agttttttca attccggtga
     6061 tattctcatt ttagccattt attatttcct tcctcttttc tacagtattt aaagataccc
     6121 caagaagcta attataacaa gacgaactcc aattcactgt tccttgcatt ctaaaacctt
     6181 aaataccaga aaacagcttt ttcaaagttg ttttcaaagt tggcgtataa catagtatcg
     6241 acggagccga ttttgaaacc gcggtgatca caggcagcaa cgctctgtca tcgttacaat
     6301 caacatgcta ccctccgcga gatcatccgt gtttcaaacc cggcagctta gttgccgttc
     6361 ttccgaatag catcggtaac atgagcaaag tctgccgcct tacaacggct ctcccgctga
     6421 cgccgtcccg gactgatggg ctgcctgtat cgagtggtga ttttgtgccg agctgccggt
     6481 cggggagctg ttggctggct ggtggcagga tatattgtgg tgtaaacaaa ttgacgctta
     6541 gacaacttaa taacacattg cggacgtttt taatgtactg aattaacgcc gaattaattc
     6601 gggggatctg gattttagta ctggattttg gttttaggaa ttagaaattt tattgataga
     6661 agtattttac aaatacaaat acatactaag ggtttcttat atgctcaaca catgagcgaa
     6721 accctatagg aaccctaatt cccttatctg ggaactactc acacattatt atggagaaac
     6781 tcgagcttgt cgatcgactc tagctagagg atcgatccga accccagagt cccgctcaga
     6841 agaactcgtc aagaaggcga tagaaggcga tgcgctgcga atcgggagcg gcgataccgt
     6901 aaagcacgag gaagcggtca gcccattcgc cgccaagctc ttcagcaata tcacgggtag
     6961 ccaacgctat gtcctgatag cggtccgcca cacccagccg gccacagtcg atgaatccag
     7021 aaaagcggcc attttccacc atgatattcg gcaagcaggc atcgccatgt gtcacgacga
     7081 gatcctcgcc gtcgggcatg cgcgccttga gcctggcgaa cagttcggct ggcgcgagcc
     7141 cctgatgctc ttcgtccaga tcatcctgat cgacaagacc ggcttccatc cgagtacgtg
     7201 ctcgctcgat gcgatgtttc gcttggtggt cgaatgggca ggtagccgga tcaagcgtat
     7261 gcagccgccg cattgcatca gccatgatgg atactttctc ggcaggagca aggtgagatg
     7321 acaggagatc ctgccccggc acttcgccca atagcagcca gtcccttccc gcttcagtga
     7381 caacgtcgag cacagctgcg caaggaacgc ccgtcgtggc cagccacgat agccgcgctg
     7441 cctcgtcctg gagttcattc agggcaccgg acaggtcggt cttgacaaaa agaaccgggc
     7501 gcccctgcgc tgacagccgg aacacggcgg catcagagca gccgattgtc tgttgtgccc
     7561 agtcatagcc gaatagcctc tccacccaag cggccggaga acctgcgtgc aatccatctt
     7621 gttcaatccc catggtcgat cgacagatct gcgaaagctc gagagagata gatttgtaga
     7681 gagagactgg tgatttcagc gtgtcctctc caaatgaaat gaacttcctt atatagagga
     7741 agggtcttgc gaaggatagt gggattgtgc gtcatccctt acgtcagtgg agatatcaca
     7801 tcaatccact tgctttgaag acgtggttgg aacgtcttct ttttccacga tgctcctcgt
     7861 gggtgggggt ccatctttgg gaccactgtc ggcagaggca tcttgaacga tagcctttcc
     7921 tttatcgcaa tgatggcatt tgtaggtgcc accttccttt tctactgtcc ttttgatgaa
     7981 gtgacagata gctgggcaat ggaatccgag gaggtttccc gatattaccc tttgttgaaa
     8041 agtctcaata gccctttggt cttctgagac tgtatctttg atattcttgg agtagacgag
     8101 agtgtcgtgc tccaccatgt tcacatcaat ccacttgctt tgaagacgtg gttggaacgt
     8161 cttctttttc cacgatgctc ctcgtgggtg ggggtccatc tttgggacca ctgtcggcag
     8221 aggcatcttg aacgatagcc tttcctttat cgcaatgatg gcatttgtag gtgccacctt
     8281 ccttttctac tgtccttttg atgaagtgac agatagctgg gcaatggaat ccgaggaggt
     8341 ttcccgatat taccctttgt tgaaaagtct caatagccct ttggtcttct gagactgtat
     8401 ctttgatatt cttggagtag acgagagtgt cgtgctccac catgttggca agctgctcta
     8461 gccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga
     8521 caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac
     8581 tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt
     8641 gagcggataa caatttcaca caggaaacag ctatgacatg attacgaatt cgagctcggt
     8701 acccggggat cggcgcgccc tatggaggtc aacatggtgg agcacgacac tctggtctac
     8761 tccaaaaatg tcaaagatac agtctcagaa gatcaaaggg ctattgagac ttttcaacaa
     8821 aggataattt cgggaaacct cctcggattc cattgcccag ctatctgtca cttcatcgaa
     8881 aggacagtag aaaaggaagg tggctcctac aaatgccatc attgcgataa aggaaaggct
     8941 atcattcaag atctctctgc cgacagtggt cccaaagatg gacccccacc cacgaggagc
     9001 atcgtggaaa aagaagaggt tccaaccacg tctacaaagc aagtggattg atgtgacatc
     9061 tccactgacg taagggatga cgcacaatcc cactatcctt cgcaagaccc ttcctctata
     9121 taaggaagtt catttcattt ggagaggaca cgctcgagta taagagctca tttttacaac
     9181 aattaccaac aacaacaaac aacaaacaac attacaatta catttacaat tatcgataca
     9241 atggtgcagg acacttcaag tgctagcaca agtccaattt taaccagatg gtatatagac
     9301 actcgacctc tcacggcaag taccgccgca cttccgctgc tggaaacgct gcaaccagct
     9361 gatcagatct ccgtccaaaa atattatcat ctcaaggaca agcacatgtc acttgcctcc
     9421 aaccttttga agtatttatt cgttcatcga aattgccgaa taccatggag tagtattgtc
     9481 atatcaagaa cgcccgaccc gcacagaagg ccttgctata tacctccttc aggttcacaa
     9541 gaggactcct tcaaagacgg atatacggga atcaacgtag aattcaacgt tagtcaccaa
     9601 gccagcatgg tggcaatcgc aggtacggct ttcacaccta atagcggtgg agatagcaaa
     9661 ctcaaaccgg aggtcggaat agatataaca tgtgtaaatg agaggcaggg caggaatgga
     9721 gaagaaagat ccttggaatc cctgaggcag tatatagata tattcagcga agtattcagc
     9781 accgcagaga tggctaatat aaggcgactt gatggggttt cctcaagttc ccttagtgca
     9841 gacagactcg tagattatgg ataccgatta ttctacacct attgggcctt aaaagaggct
     9901 tatatcaaga tgacaggtga ggccttattg gctccttggt tgagagagct ggagttttcc
     9961 aacgtggtcg ctccagccgc tgtcgccgaa agcggggact ctgctggaga ctttggcgaa
    10021 ccgtacactg gcgtgcgtac aacattgtat aagaacctcg tagaggacgt acgaattgag
    10081 gtagctgctt tgggaggaga ttatctgttc gcaacagccg ctagaggtgg tggtataggc
    10141 gcatcctcac gtcctggtgg tggcccagat ggctctggaa ttagatccca ggacccttgg
    10201 aggccgttca agaaactcga tatagagcgt gacatacagc catgtgctac gggcgtttgt
    10261 aattgcctgt cctcaggata ggcttgatcc cccgtcgaca gctagctata tcatcaattt
    10321 atgtattaca cataatatcg cactcagtct ttcatctacg gcaatgtacc agctgatata
    10381 atcagttatt gaaatatttc tgaatttaaa cttgcatcaa taaatttatg tttttgcttg
    10441 gactataata cctgacttgt tattttatca ataaatattt aaactatatt tctttcaaga
    10501 tcgctgttag agctcggact ggcagacata ctgtcccaca aatgaagatg gaatctgtaa
    10561 aagaaaacgc gtgaaataat gcgtctgaca aaggttaggt cggctgcctt taatcaatac
    10621 caaagtggtc cctaccacga tggaaaaact gtgcagtcgg tttggctttt tctgacgaac
    10681 aaataagatt cgtggccgac aggtgggggt ccaccatgtg aaggcatctt cagactccaa
    10741 taatggagca atgacgtaag ggcttacgaa ataagtaagg gtagtttggg aaatgtccac
    10801 tcacccgtca gtctataaat acttagcccc tccctcattg ttaagggagc aaaatctcag
    10861 agagatagtc ctagagagag aaagagagca agtagcctag aagtagtcaa ggcggcgaag
    10921 tattcaggca cgtggccagg aagaagaaaa gccaagacga cgaaaacagg taagagctaa
    10981 gcttaatggc atcctttgaa aattcactca gtgtcttaat agttggggct ggcttaggtg
    11041 gtttggccgc tgctatagca ctgcgaaggc aaggacatgt agtcaagata tacgatagtt
    11101 cttcattcaa ggccgaattg ggagcaggat tagctgtacc acccaatact ctccgttccc
    11161 tgcaacaact gggctgtaat acagaaaacc tgaacggagt agacaacctc tgctttacgg
    11221 ccatgggata cgacgggagt gtaggtatga tgaataatat gaccgactac cgtgaggcat
    11281 acggcacttc ctggataatg gtacatcgag tggatctcca caacgagctg atgcgagtcg
    11341 ccttggatcc gggcgggtta gggcctcctg ccactcttca ccttaaccat cgtgttacgt
    11401 tctgcgatgt ggatgcctgt acagtcacgt ttacgaacgg gacgacacag tctgctgact
    11461 tgattgtcgg agctgatggt attaggtcta cgattcgaag attcgttctg gaggaggacg
    11521 tgactgttcc agctagcgga attgtcggct ttcgatggtt ggtccaggcc gatgctctcg
    11581 acccatatcc cgaactggat tggattgtca aaaaaccacc cttgggagcc aggttgattt
    11641 ctactccaca gaacccgcag agtggcgtag gactggccga tagacgaacg atcattattt
    11701 acgcatgcag aggtgggacc atggttaatg tattagccgt acacgacgac gaaagagatc
    11761 aaaacactgc tgattggtcc gttcccgcat caaaggatga tttattcaga gtgtttcacg
    11821 attaccatcc aagattccga cgactcttag agttagcaca ggacattaat ttatggcaga
    11881 tgagggtggt cccggtcttg aaaaaatggg tgaacaagag ggtatgcttg ctgggtgatg
    11941 ccgcccatgc ttcactgccc acacttggac aaggcttcgg gatggggttg gaggatgcag
    12001 tcgctttggg cacgttgttg cctaagggaa cgaccgcatc acagatcgag acaaggctgg
    12061 ccgtttacga acagctgcga aaggatcgag ctgagtttgt cgccgcagag agctatgagg
    12121 agcaatatgt cccagagatg agagggctgt accttaggtc caaagaattg agagacagag
    12181 tgatgggtta tgatattaag gtagagtcag agaaggtttt ggaaacactg ttgcgatcat
    12241 ccaactcagc ttgagcttgt cctgctttaa tgagatatgc gagaagccta tgatcgcatg
    12301 atatttgctt tcaattctgt tgtgcacgtt gtaaaaaacc tgagcatgtg tagctcagat
    12361 ccttaccgcc ggtttcggtt cattctaatg aatatatcac ccgttactat cgtattttta
    12421 tgaataatat tctccgttca atttactgat tgtaccctac tacttatatg tacaatatta
    12481 aaatgaaaac aatatattgt gctgaatagg tttatagcga catctatgat agagcgccac
    12541 aataacaaac aattgcgttt tattattaca aatccaattt taaaaaaagc ggcagaaccg
    12601 gtcaaaccta aaagactgat tacataaatc ttattcaaat ttcaaaagtg ccccaggggc
    12661 tagtatctac gacacaccga gcggcgaact aataacgctc actgaaggga actccggttc
    12721 cccgccggcg cgcatgggtg agattccttg aagttgagta ttggccgtcc gctctaccga
    12781 aagttacggg caccattcaa cccggtccag cacggcggcc gggtaaccga cttgctgccc
    12841 cgagaattat gcagcatttt tttggtgtat gtgggcccca aatgaagtgc aggtcaaacc
    12901 ttgacagtga cgacaaatcg ttgggcgggt ccagggcgaa ttttgcgaca acatgtcgag
    12961 gctcagcagg acccggggcg cgccctatgg agtggcagac atactgtccc acaaatgaag
    13021 atggaatctg taaaagaaaa cgcgtgaaat aatgcgtctg acaaaggtta ggtcggctgc
    13081 ctttaatcaa taccaaagtg gtccctacca cgatggaaaa actgtgcagt cggtttggct
    13141 ttttctgacg aacaaataag attcgtggcc gacaggtggg ggtccaccat gtgaaggcat
    13201 cttcagactc caataatgga gcaatgacgt aagggcttac gaaataagta agggtagttt
    13261 gggaaatgtc cactcacccg tcagtctata aatacttagc ccctccctca ttgttaaggg
    13321 agcaaaatct cagagagata gtcctagaga gagaaagaga gcaagtagcc tagaagtagt
    13381 caaggcggcg aagtattcag gcacgtggcc aggaagaaga aaagccaaga cgacgaaaac
    13441 aggtaagagc taagcttaat gaatagtagt aaaaaccctc catccactct gttagatgtt
    13501 tttctggata ccgctaggaa ccttgataca gcatccagaa atgtcctgga gtgcggagaa
    13561 cacaggtgga gttacagaga gttggacaca gtgtcatccg cattggccca acaccttcga
    13621 tacactgtgg gtcttagccc cactgtagcc gtgattagcg agaatcaccc ctacatactt
    13681 gcattaatgc tcgccgtatg gaaactcggc ggaacgttcg caccgataga tgttcattcc
    13741 ccagccgaac ttgttgctgg aatgttgaat attgtaagcc cgtcatgtct tgtcattcct
    13801 agctctgatg tgacaaatca gacattggca tgcgatctta atataccggt tgttgcattc
    13861 cacccacatc aaagtacaat tcccgaactc aacaagaagt atctcacaga ttctcagatc
    13921 agtcctgacc tgcccttccc cgaccctaat cgacctgctt tgtacttatt cacgtcatct
    13981 gcaacgtcta ggtccaattt gaagtgcgtg ccacttacac acacattcat attgcgaaac
    14041 tccttaagta aacgagcatg gtgcaaaaga atgaggccag agacagattt tgatggtatt
    14101 agggtcctgg ggtgggcccc ctggagccat gtcctcgctc atatgcagga cattggccct
    14161 cttacattat tgaatgccgg gtgctacgta tttgccacga ccccatctac ctacccgacg
    14221 gagctgaagg atgaccgtga tgttattagt tgcgccgcaa atgccgtaat gtacaaaggt
    14281 gttaaatcct tcgcatgcct cccattcgtg cttgggggac tcaaggcttt atgtgagtca
    14341 gagcccagtg ttaaggcaca actgcaagtg gaggaacgag cccagctttt gaagtcactc
    14401 caacacatga aaatcctgga atgcgggggg gcaatgctcg aagtgagtgt ggcaagctgg
    14461 gctatagaga ataggattcc tatatctatt gggattggta tgacagagac aggcggcgct
    14521 cttttcgctg ggccggtcca agcaattcaa accggcttta gcagtgaaga taaatttatc
    14581 gaggacgcaa cctacttgct ggtcaaggac gattacgaga gccacgcaga ggaggacata
    14641 aacgagggcg agttagtggt aaagtccaga atgttaccta ggggttattt gggctataac
    14701 gacccctctt tctctgttga cgatgctggt tgggttactt tcaaaactgg agatcgatat
    14761 tcagtcactc ctgacggcaa attctcctgg cttggtcgaa atactgattt cattcagatg
    14821 acgagtggtg agactctcga tccccgtccg attgagtccc tcctttgtga gtccagcctt
    14881 atatcacgag cctgtgtcat cggggataag ttcctgaacg gaccagccac agctgtatgc
    14941 gctattatag aactcgaacc aacgactgtt gagaaaggcc aggctcacag cagggatatt
    15001 gcccgaatct ttgcccccat caatcgagat cttcctcccc cgttacgtat agcatggagt
    15061 cacgtacttg tacttcagcc aagcgagaaa ataccgatga cgaaaaaggg tacaatcttc
    15121 cgtaagaaaa tcgaacaggt tttcggttcc gctttaggag gcagtagcgg cgacaactca
    15181 caagcaacca ctgacgcttc agtagttagg agggatgagt tgagcaacac ggtcaaacat
    15241 atcataagca gagttttggg agtctccgac gatgaactcc tttggacact gagctttgct
    15301 gagcttggca tgacgtccgc tcttgctact agaatagcaa atgaattgaa cgaagttctg
    15361 gtaggtgtga acctccccat caatgcatgc tacattcatg tggacctgcc ttctttgtca
    15421 aatgccgtgt atgcaaagtt agcacacctt aaactcccgg ataggacgcc cgagccaagg
    15481 aaggccccag ttgaaaatcc cggtggaaag gaaatcgtaa tagtaggtca ggcttttagg
    15541 cttccgggga gtataaatga tgtcgcttct ttgagggacg cttttttggc tagacaagca
    15601 tccagtatta ttactgaaat accgccagat agatgggacc acgcatcatt ttatccaaaa
    15661 gacatacgat ttaataaagc agggctcgtc gacatcgcta actacgacca cagttttttc
    15721 ggattgacag ccacggaggc actgtacctg tcaccaacga tgcgtttggc acttgaagtg
    15781 tcctttgaag ccttggagaa tgctaatata cccgtgtccc agttaaaggg ctctcaaaca
    15841 gctgtatatg tcgcaaccac cgacgacggg tttgagacac tgctgaatgc tgaggcagga
    15901 tatgatgcat acacacgatt ctatggcact ggtagggccg cctctacggc cagtggacga
    15961 attagttatt tactcgacgt acacggccct tcaattaccg tagacactgc ttgttcaggc
    16021 ggtgctgttt gcatagacca ggccatagac tacctccaat cctctagcgc agctgatacc
    16081 gccataatct gcgccagcaa cactcactgc tggccaggtt ccttcatgtt tctgagtgct
    16141 caaggtatgg taagttccgg ggggcgatgt gcaactttta cgacggatgc cgacggctat
    16201 gtaccctctg aaggtgctgt ggcatttatc ctcaaaaccc gagaagcagc tatgagagat
    16261 aaagatacta tcttggcaac tatcaaagca acgcagatta gtcataatgg tcgttcccaa
    16321 ggtttagtcg caccaaacgt caattcacaa gctgatttac acagaagctt attgcagaaa
    16381 gctggtttga gtccagccga cattcacttc atcgaagctc acgggacagg aacgtcactc
    16441 ggtgacctta gtgaaataca agcaattaat gatgcctaca cgtcatctca gcctcgaaca
    16501 gccggaccct taatagtaag cgcaagtaaa accgtcattg gtcacacaga gcctgctgga
    16561 ccgttggtag gcatgctctc agttctcaac tcctttaaag agggcgcagt tcccggctta
    16621 gctcacctga cagcagataa cctcaatccg gctttagact gctcctccgt ccctttactt
    16681 atcccctacc agcctgtcca cttggcagct cctaagccac acagggctgc tgtgatgagt
    16741 tatggcttct ccggcactct tggaggtata gttcttgaag caccggatga ggaaaggctt
    16801 gaggaagaac cacccaacga taagcctatg ttatttgttg ttagcgccaa aactcatact
    16861 gcattgattg agtaccttgg acgatacctt gaatttctgc tccaggccaa tccccaagac
    16921 ttctgcgata tctgttacac tagctgcgtt ggacgtgagc attacaggta ccgttttgca
    16981 tgtgtggcaa acgatatgga agatttaatt ggccaacttc agaagaggct tgggtctaag
    17041 gttccaccta aaccgtccta taaaagagga gccctcgctt ttgctttcag tggacaaggg
    17101 acccagtttc gtggaatggc aacagaattg gctaaggcat atagtggttt tcgtaagata
    17161 gtcagtgatc ttgctaaaag agcatcagaa ctgtccggcc atgcaatcga caggtttctg
    17221 cttgcatatg acattggggc tgagaatgtt gcaccggata gtgaagctga tcaaatctgc
    17281 atattcgttt accaatgtag tgttctcaga tggctccaaa caatggggat tcgtccgtca
    17341 gccgtgatcg gtcacagcct cggtgagatt agcgcatccg tagctgctgg agcattgtcc
    17401 ttggactctg cccttgacct cgtaataagt agagccaggt tactgaggtc atctacgaat
    17461 gccccagctg gcatggcagc tatgtctgca tcccaggatg aagtagtcga actcatagga
    17521 aagctcgatt tagataaagc taatagcctc agcgtgtccg taataaacgg cccccagaac
    17581 accgttgttt caggatcttc tgcagcaata gaaagtatcg ttgctctcgc aaagggacga
    17641 aaaatcaagg ccagcgcact caacattaac caggcattcc atagtcctta cgtcgattct
    17701 gcagtacccg gcttacgagc ttggagtgag aaacatatat catcagctcg accattgcaa
    17761 atccctttgt actctaccct cttgggcgct caagtttctg agggtcaaat gttaaatccc
    17821 gatcactggg tagaccatgc taggaaaccc gttcagttcg cacaagcagc cactataatg
    17881 aaggaatctt tcaccggagt aataatcgat attggacctc aagttgtagc atggtccctg
    17941 ctcttaagca atggtcttac ttccgtgacc gcactcgccg caaagcgtgg ccgttcacag
    18001 caagtggcct tcctctccgc cttagctgat ctttatcagg actacggcgt cgtaccagac
    18061 tttgttggac tgtacgcaca acaggaggac gcttccagac tgaaaaagac cgatatctta
    18121 acttacccct ttcagagggt tcgacgttac ccttccttta taccctctag aagggcaccc
    18181 acccacgctc atgtccaaga cgaggaaact ctgtcatcag gatcatcaac tccaacctta
    18241 gaaaatactg acctggacag tgggaaggaa tctctgatgg gaccaacaag agggcttttg
    18301 cgagtcgatg atcttaggga ctcaatagtc agttcagtca aagatgtcct ggaattgaag
    18361 agtaacgaag atctggacct cagcgagtcc cttaatgctc ttggcatgga ctctataatg
    18421 ttcgctcaat tacgaaaaag gataggggag ggcctcggcc tgtccgttcc aatggttttt
    18481 ctcagcgatg ccttcagtat tggagaaatg gtcagtaacc tcgtggaaca ggccgaagct
    18541 tccgaggaca attgagctta tatgaagatg aagatgaaat atttggtgtg tcaaataaaa
    18601 agcttgtgtg cttaagtttg tgtttttttc ttggcttgtt gtgttatgaa tttgtggctt
    18661 tttctaatat taaatgaatg taagatcaca ttataatgaa taaacaaatg tttctataat
    18721 ccattgtgaa tgttttgttg gatctcttct gcagcatata actactgtat gtgctatggt
    18781 atggactatg gaatatgatt aaagataagc gctgttagag ctctcccggc gcgccctatg
    18841 gagagatttg ccttttcaat ttcagaaaga atgctaaccc acagatggtt agagaggctt
    18901 acgcagcagg tatcatcaag acgatctacc cgagcaataa tctccaggaa atcaaatacc
    18961 ttcccaagaa ggttaaagat gcagtcaaaa gattcaggac taactgcatc aagaacacag
    19021 agaaagatat atttctcaag atcagaagta ctattccagt atggacgatt caaggcttgc
    19081 ttcacaaacc aaggcaagta atagagattg gagtctctaa aaaggtagtt cccactgaat
    19141 caaaggccat ggagtcaaag attcaaatag aggacctaac agaactcgcc gtaaagactg
    19201 gcgaacagtt catacagagt ctcttacgac tcaatgacaa gaagaaaatc ttcgtcaaca
    19261 tggtggagca cgacacactt gtctactcca aaaatatcaa agatacagtc tcagaagacc
    19321 aaagggcaat tgagactttt caacaaaggg taatatccgg aaacctcctc ggattccatt
    19381 gcccagctat ctgtcacttt attgtgaaga tagtggaaaa ggaaggtggc tcctacaaat
    19441 gccatcattg cgataaagga aaggccatcg ttgaagatgc ctctgccgac agtggtccca
    19501 aagatggacc cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt
    19561 caaagcaagt ggattgatgt gatatctcca ctgacgtaag ggatgacgca caatcgcctg
    19621 gacttccaca gctatagcgg gccactatcc ttcgcaagac ccttcctcta tataaggaag
    19681 ttcatttcat ttggagagaa cacgggggac taatgcgtat aaatatctct ctgtctagct
    19741 tattcgagag attaagcaaa ctcagctctc gtagcatagc tattacatgt ggtgtcgtcc
    19801 tcgcctctgc tattgctttt cccatcatac gacgagatta tcagacgttc ttagaggttg
    19861 gtcctagcta tgcccctcaa aattttcgag gctacattat cgtttgcgta ctcagcttgt
    19921 tccgacaaga gcaaaaaggg ttggcaatct acgacagact cccagagaag agaagatggc
    19981 tggccgatct cccatttcgt gaaggtacta gacctagtat aacatcacac ataatacaga
    20041 gacagcgaac tcaacttgta gaccaagagt ttgccacacg agaactgata gacaaagtta
    20101 ttcccagggt gcaagcacga catacagata aaacattttt gagcacgagc aagtttgaat
    20161 ttcatgctaa agccattttc ctgttgccaa gtattcctat aaacgacccc ttaaacatac
    20221 ctagtcatga tacggttcgt agaacgaaac gagagattgc acacatgcat gactaccacg
    20281 actgtactct tcacttagct cttgcagctc aagatggaaa agaagtcctg aaaaaaggct
    20341 ggggccaacg tcatccgctt gccgggccgg gtgtacctgg gccgccgaca gaatggactt
    20401 tcttatacgc accccgtaat gaggaagaag ctagggtagt agaaatgatt gtcgaggcaa
    20461 gtatcggata catgactaat gatccggcag gtaaaatcgt cgagaatgcc aaatgagctt
    20521 gtcctgcttt aatgagatat gcgagaagcc tatgatcgca tgatatttgc tttcaattct
    20581 gttgtgcacg ttgtaaaaaa cctgagcatg tgtagctcag atccttaccg ccggtttcgg
    20641 ttcattctaa tgaatatatc acccgttact atcgtatttt tatgaataat attctccgtt
    20701 caatttactg attgtaccct actacttata tgtacaatat taaaatgaaa acaatatatt
    20761 gtgctgaata ggtttatagc gacatctatg atagagcgcc acaataacaa acaattgcgt
    20821 tttattatta caaatccaat tttaaaaaaa gcggcagaac cggtcaaacc taaaagactg
    20881 attacataaa tcttattcaa atttcaaaag tgccccaggg gctagtatct acgacacacc
    20941 gagcggcgaa ctaataacgc tcactgaagg gaactccggt tccccgccgg cgcgcatggg
    21001 tgagattcct tgaagttgag tattggccgt ccgctctacc gaaagttacg ggcaccattc
    21061 aacccggtcc agcacggcgg ccgggtaacc gacttgctgc cccgagaatt atgcagcatt
    21121 tttttggtgt atgtgggccc caaatgaagt gcaggtcaaa ccttgacagt gacgacaaat
    21181 cgttgggcgg gtccagggcg aattttgcga caacatgtcg aggctcagca ggaccgctgt
    21241 tagagctcag tg
//
