LOCUS       sequence-270374-        3827 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Module plasmid, individual component of Fungal Bioluminescent 
            Pathway, Npga.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3827)
  AUTHORS   Khakhar A, Starker CG, Chamness JC, Lee N, Stokke S, Wang C, Swanson
            R, Rizvi F, Imaizumi T, Voytas DF
  TITLE     Building customizable auto-luminescent luciferase-based reporters in
            plants.
  JOURNAL   Elife. 2020 Mar 25;9. pii: 52786. doi: 10.7554/eLife.52786.
  PUBMED    32209230
REFERENCE   2  (bases 1 to 3827)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2020-03-25"; volume: "9. pii: 52786. doi"; pages: " 
            10.7554/eLife.52786"
COMMENT     Sequence Label: P195
FEATURES             Location/Qualifiers
     source          1..3827
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        109..454
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     397..417
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     terminator      1605..1805
                     /label=gene 7 terminator
                     /note="transcription terminator for octopine-type Ti 
                     plasmid gene 7"
     promoter        1880..1984
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      3052..3640
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3541..3560
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3794..3811
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cacctgctca ttgacggcgc gccctatgga ggtcaacatg gtggagcacg acactctggt
       61 ctactccaaa aatgtcaaag atacagtctc agaagatcaa agggctattg agacttttca
      121 acaaaggata atttcgggaa acctcctcgg attccattgc ccagctatct gtcacttcat
      181 cgaaaggaca gtagaaaagg aaggtggctc ctacaaatgc catcattgcg ataaaggaaa
      241 ggctatcatt caagatctct ctgccgacag tggtcccaaa gatggacccc cacccacgag
      301 gagcatcgtg gaaaaagaag aggttccaac cacgtctaca aagcaagtgg attgatgtga
      361 catctccact gacgtaaggg atgacgcaca atcccactat ccttcgcaag acccttcctc
      421 tatataagga agttcatttc atttggagag gacacgctcg agtataagag ctcattttta
      481 caacaattac caacaacaac aaacaacaaa caacattaca attacattta caattatcga
      541 tacaatggtg caggacactt caagtgctag cacaagtcca attttaacca gatggtatat
      601 agacactcga cctctcacgg caagtaccgc cgcacttccg ctgctggaaa cgctgcaacc
      661 agctgatcag atctccgtcc aaaaatatta tcatctcaag gacaagcaca tgtcacttgc
      721 ctccaacctt ttgaagtatt tattcgttca tcgaaattgc cgaataccat ggagtagtat
      781 tgtcatatca agaacgcccg acccgcacag aaggccttgc tatatacctc cttcaggttc
      841 acaagaggac tccttcaaag acggatatac gggaatcaac gtagaattca acgttagtca
      901 ccaagccagc atggtggcaa tcgcaggtac ggctttcaca cctaatagcg gtggagatag
      961 caaactcaaa ccggaggtcg gaatagatat aacatgtgta aatgagaggc agggcaggaa
     1021 tggagaagaa agatccttgg aatccctgag gcagtatata gatatattca gcgaagtatt
     1081 cagcaccgca gagatggcta atataaggcg acttgatggg gtttcctcaa gttcccttag
     1141 tgcagacaga ctcgtagatt atggataccg attattctac acctattggg ccttaaaaga
     1201 ggcttatatc aagatgacag gtgaggcctt attggctcct tggttgagag agctggagtt
     1261 ttccaacgtg gtcgctccag ccgctgtcgc cgaaagcggg gactctgctg gagactttgg
     1321 cgaaccgtac actggcgtgc gtacaacatt gtataagaac ctcgtagagg acgtacgaat
     1381 tgaggtagct gctttgggag gagattatct gttcgcaaca gccgctagag gtggtggtat
     1441 aggcgcatcc tcacgtcctg gtggtggccc agatggctct ggaattagat cccaggaccc
     1501 ttggaggccg ttcaagaaac tcgatataga gcgtgacata cagccatgtg ctacgggcgt
     1561 ttgtaattgc ctgtcctcag gataggcttg atcccccgtc gacagctagc tatatcatca
     1621 atttatgtat tacacataat atcgcactca gtctttcatc tacggcaatg taccagctga
     1681 tataatcagt tattgaaata tttctgaatt taaacttgca tcaataaatt tatgtttttg
     1741 cttggactat aatacctgac ttgttatttt atcaataaat atttaaacta tatttctttc
     1801 aagatcgctg ttagagctcg gacgtgggca ggtgtgatga aaagccttag ggtcaggtgg
     1861 cacttttcgg ggaaatgtgc gatgaacccc tatttgttta tttttctaaa tacattcaaa
     1921 tatgtatccg ctaatgagac aataaccctg ataaatgctt caattatatt gaaaaaggaa
     1981 gagtatgagt atccaacatt tccgtgtcgc ccttattccc ttttttgcgg ccttctgcct
     2041 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     2101 tgcccgagtg ggttacattg aactggatct caacagcggc aaaatcttag agagttttcg
     2161 ccccgaagaa cgcttcccaa tgatgagcac tttcaaagtt ctgctatgtg gcgcggtgtt
     2221 atcccgtatt gatgctgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     2281 cttggttgag tattcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     2341 attatgtagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     2401 gattggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     2461 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     2521 gatgcctgta gcaatggcaa caactctccg caaactatta actggcgaac tacttactct
     2581 tgcttcacgc cagcaactca ttgactggat ggaggcggac aaagttgcag gaccacttct
     2641 gcgctcggca cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     2701 ctctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     2761 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     2821 tgcctcactg attaagcatt ggtaactgtc agacgaaggg aaataaatag tagcccgcct
     2881 gatgtgcggg cttttttttg gtggtggtta ccagtgtcgc ttagctaccg taggtcctct
     2941 taggcggtcc gattagcgac atagtcatca atgccttgac ggcaaaatcc cttaacgtga
     3001 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     3061 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     3121 ttgtttgccg gatcaagagc tactaactct ttttccgaag gtaactggct tcagcagagc
     3181 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     3241 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     3301 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     3361 gtcgggctga acggggggtt cgtgctcaca gcccagcttg gagcgaacga cctacaccga
     3421 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     3481 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     3541 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     3601 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     3661 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     3721 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
     3781 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcc
//
