LOCUS       sequence-270378-        3905 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Module plasmid, individual component of Fungal Bioluminescent 
            Pathway, CPH.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3905)
  AUTHORS   Khakhar A, Starker CG, Chamness JC, Lee N, Stokke S, Wang C, Swanson
            R, Rizvi F, Imaizumi T, Voytas DF
  TITLE     Building customizable auto-luminescent luciferase-based reporters in
            plants.
  JOURNAL   Elife. 2020 Mar 25;9. pii: 52786. doi: 10.7554/eLife.52786.
  PUBMED    32209230
REFERENCE   2  (bases 1 to 3905)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2020-03-25"; volume: "9. pii: 52786. doi"; pages: " 
            10.7554/eLife.52786"
COMMENT     Sequence Label: P223
FEATURES             Location/Qualifiers
     source          1..3905
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    677..732
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     promoter        1958..2062
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      3130..3718
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3619..3638
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3872..3889
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cacctgctca tgatcggcgc gccctatgga gatatgactg gtaagttttt cttgccaata
       61 cgaattagaa aacatgtctt tgaagatgaa ctgtattttt tttttttact ttgttgtcat
      121 tttaatgtac tttcttatca ggattaaatc ttctgtaatt tagagtagtt tttttaacaa
      181 gataattaac aaacttagag taatgaaaat tgagatgttc agttttcact catatttcac
      241 attttggtga aagagtgggt agtatgcaac gttctaagta tgtttggact ttgtatcatg
      301 ttgttttgat tctttgacga catgtctatt tgggaaacac caatgacgtg taccttgaga
      361 ctgatacgat tcaaagggat agaaacacgt cagatttaca agtggcacct cttcaatgga
      421 caatgggtat tccaatatgc taagatgcta cgagatatct aatttatcta acacaactca
      481 attccaaacc aaaaatctga tgccagctcg acaagacaaa aaatctaagc tcaaaaatgt
      541 caacaaccaa tagaaatcaa ggcattgacg atatcacgag ataagcaaat taaatcttca
      601 agttttgcaa ttcatatgta cgttataaat acccaaaaac ctcaccgtaa cctagctatc
      661 ctactgtatt tttacaacaa ttaccaacaa caacaaacaa caaacaacat tacaattact
      721 atttacaatt acaatggctc ctatctcctc cacctggtcc agattaatca ggttcgtagc
      781 tatagaaacg tcactcgtgc atataggcga accgattgat gcaactatgg acgtagggct
      841 tgctcgtcga gaagggaaaa ctattcaggc ctatgagatt atcggatctg gcagcgccct
      901 ggacctttct gcacaggtga gtaagaacgt gctcacagta cgagaattgt tgatgccact
      961 ttctagagaa gagataaaga cggtcagatg cttaggcctt aactatcctg tccacgcaac
     1021 agaagccaat gttgctgtac cgaagtttcc gaacctgttc tacaaaccag tcacaagtct
     1081 catcgggcct gatggtctta tcacgatccc tagtgtagtg caaccgccta aggaacatca
     1141 gtcagattat gaagctgaac ttgtgattgt aatcggaaag gccgccaaga acgtgtctga
     1201 ggacgaagcc ttggattatg tcttaggcta tacagctgct aacgatatta gtttccgaaa
     1261 acaccagctc gcagtctcac agtggtcatt ctcaaaagga tttggatctc tccttctcac
     1321 tatacgtatg gcccagacac atagtggaaa cataaatagg ttctcacgtg atcaaatctt
     1381 taatgttaag aaaacaatca gctttttgtc tcaaggaact accctcgaac ctgggtctat
     1441 cattttgact ggcacccctg atggggtcgg cttcgtcagg aacccgccct tatacttaaa
     1501 agatggggat gaagtgatga cttggatagg ttccggcatt ggtacgcttg ctaacaccgt
     1561 tcaagaagag aaaacatgct ttgcaagcgg tgggcatgag taggctttta tgttggtgat
     1621 atggtggtaa atgtagggat ttagtttaca attgcgtatg tctgtgttgg atatctgtag
     1681 tgctgttctt atggcttaga tcttgtaatt tctcattaca gtatcaatga atagatatca
     1741 gtttctagtg atgacattgg ttcgtctttt agctgttgat taatttttct taattgattc
     1801 atcctattgc aattcttctg aatttaaatt gtatactgtg aaattaagaa aattcttgaa
     1861 attaatgaga atttgagtaa tagcgctgtt agagctctga cgtgggcagg tgtgatgaaa
     1921 agccttaggg tcaggtggca cttttcgggg aaatgtgcga tgaaccccta tttgtttatt
     1981 tttctaaata cattcaaata tgtatccgct aatgagacaa taaccctgat aaatgcttca
     2041 attatattga aaaaggaaga gtatgagtat ccaacatttc cgtgtcgccc ttattccctt
     2101 ttttgcggcc ttctgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
     2161 tgctgaagat cagttgggtg cccgagtggg ttacattgaa ctggatctca acagcggcaa
     2221 aatcttagag agttttcgcc ccgaagaacg cttcccaatg atgagcactt tcaaagttct
     2281 gctatgtggc gcggtgttat cccgtattga tgctgggcaa gagcaactcg gtcgccgcat
     2341 acactattct cagaatgact tggttgagta ttcaccagtc acagaaaagc atcttacgga
     2401 tggcatgaca gtaagagaat tatgtagtgc tgccataacc atgagtgata acactgcggc
     2461 caacttactt ctgacaacga ttggaggacc gaaggagcta accgcttttt tgcacaacat
     2521 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     2581 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca actctccgca aactattaac
     2641 tggcgaacta cttactcttg cttcacgcca gcaactcatt gactggatgg aggcggacaa
     2701 agttgcagga ccacttctgc gctcggcact tccggctggc tggtttattg ctgataaatc
     2761 tggagccggt gagcgtggct ctcgcggtat cattgcagca ctggggccag atggtaagcc
     2821 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     2881 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag acgaagggaa
     2941 ataaatagta gcccgcctga tgtgcgggct tttttttggt ggtggttacc agtgtcgctt
     3001 agctaccgta ggtcctctta ggcggtccga ttagcgacat agtcatcaat gccttgacgg
     3061 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     3121 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     3181 accgctacca gcggtggttt gtttgccgga tcaagagcta ctaactcttt ttccgaaggt
     3241 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     3301 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     3361 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     3421 accggataag gcgcagcggt cgggctgaac ggggggttcg tgctcacagc ccagcttgga
     3481 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     3541 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     3601 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     3661 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     3721 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     3781 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     3841 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
     3901 gcgcc
//
