LOCUS       sequence-270380-        4054 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Module plasmid, individual component of Fungal Bioluminescent 
            Pathway, Npga.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4054)
  AUTHORS   Khakhar A, Starker CG, Chamness JC, Lee N, Stokke S, Wang C, Swanson
            R, Rizvi F, Imaizumi T, Voytas DF
  TITLE     Building customizable auto-luminescent luciferase-based reporters in
            plants.
  JOURNAL   Elife. 2020 Mar 25;9. pii: 52786. doi: 10.7554/eLife.52786.
  PUBMED    32209230
REFERENCE   2  (bases 1 to 4054)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2020-03-25"; volume: "9. pii: 52786. doi"; pages: " 
            10.7554/eLife.52786"
COMMENT     Sequence Label: P366
FEATURES             Location/Qualifiers
     source          1..4054
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      225..813
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     714..733
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     967..984
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      2832..3032
                     /label=gene 7 terminator
                     /note="transcription terminator for octopine-type Ti 
                     plasmid gene 7"
     promoter        3107..3211
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctgattaagc attggtaact gtcagacgaa gggaaataaa tagtagcccg cctgatgtgc
       61 gggctttttt ttggtggtgg ttaccagtgt cgcttagcta ccgtaggtcc tcttaggcgg
      121 tccgattagc gacatagtca tcaatgcctt gacggcaaaa tcccttaacg tgagttttcg
      181 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
      241 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
      301 ccggatcaag agctactaac tctttttccg aaggtaactg gcttcagcag agcgcagata
      361 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
      421 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
      481 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
      541 tgaacggggg gttcgtgctc acagcccagc ttggagcgaa cgacctacac cgaactgaga
      601 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
      661 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
      721 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
      781 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
      841 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
      901 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
      961 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc cacctgctca ttgacggcgc
     1021 gccctatgga gaattcatgg ggcattacaa ttaattagta atactaattt taataacact
     1081 aaatcatatt agtctctata ggtataatat ataactctgg agtcactatc tgtatttggg
     1141 atcaacagtt acaaaggtac gagtaaagtc tctgatccga caaacaatcc gtatatccta
     1201 ccttcccgaa atttcatgta taaaattata ctaaaatatt atttttcttg tattatccga
     1261 ttgaaaatta tgcgaattca acataaaact ctaaaaaaag aaaataatga aaattacaac
     1321 ttaaaagagt gtctaagttg gaatcatggt attacataaa gttgaggaca atataagcaa
     1381 gcaaacaagt actctagcta tcaatttact ttggactact atatgataaa tatttcaacc
     1441 cccctcctcc cccaccaaaa aaaaattaaa ataacaagtt gaaggactca aaaaaaaaaa
     1501 aaaaaaaaac tcaaaaccaa cctcaatcat acattcatat cctcttccta cccccatctt
     1561 ggatgagata agattaacga ggtgcttaca cgtgtcacct ctattgtggt gacttaaaaa
     1621 aaattccaac ctttcatatg tagatattaa gtaattgtat aatgttatca agaaccacat
     1681 aacatatcaa aaaccttatc atttcattat ataaaaggat agtggacatc aaaaggttca
     1741 tattgaacca aaaaaagaga gaagaagcaa aatggtgcag gacacttcaa gtgctagcac
     1801 aagtccaatt ttaaccagat ggtatataga cactcgacct ctcacggcaa gtaccgccgc
     1861 acttccgctg ctggaaacgc tgcaaccagc tgatcagatc tccgtccaaa aatattatca
     1921 tctcaaggac aagcacatgt cacttgcctc caaccttttg aagtatttat tcgttcatcg
     1981 aaattgccga ataccatgga gtagtattgt catatcaaga acgcccgacc cgcacagaag
     2041 gccttgctat atacctcctt caggttcaca agaggactcc ttcaaagacg gatatacggg
     2101 aatcaacgta gaattcaacg ttagtcacca agccagcatg gtggcaatcg caggtacggc
     2161 tttcacacct aatagcggtg gagatagcaa actcaaaccg gaggtcggaa tagatataac
     2221 atgtgtaaat gagaggcagg gcaggaatgg agaagaaaga tccttggaat ccctgaggca
     2281 gtatatagat atattcagcg aagtattcag caccgcagag atggctaata taaggcgact
     2341 tgatggggtt tcctcaagtt cccttagtgc agacagactc gtagattatg gataccgatt
     2401 attctacacc tattgggcct taaaagaggc ttatatcaag atgacaggtg aggccttatt
     2461 ggctccttgg ttgagagagc tggagttttc caacgtggtc gctccagccg ctgtcgccga
     2521 aagcggggac tctgctggag actttggcga accgtacact ggcgtgcgta caacattgta
     2581 taagaacctc gtagaggacg tacgaattga ggtagctgct ttgggaggag attatctgtt
     2641 cgcaacagcc gctagaggtg gtggtatagg cgcatcctca cgtcctggtg gtggcccaga
     2701 tggctctgga attagatccc aggacccttg gaggccgttc aagaaactcg atatagagcg
     2761 tgacatacag ccatgtgcta cgggcgtttg taattgcctg tcctcaggat aggcttgatc
     2821 ccccgtcgac agctagctat atcatcaatt tatgtattac acataatatc gcactcagtc
     2881 tttcatctac ggcaatgtac cagctgatat aatcagttat tgaaatattt ctgaatttaa
     2941 acttgcatca ataaatttat gtttttgctt ggactataat acctgacttg ttattttatc
     3001 aataaatatt taaactatat ttctttcaag atcgctgtta gagctcggac gtgggcaggt
     3061 gtgatgaaaa gccttagggt caggtggcac ttttcgggga aatgtgcgat gaacccctat
     3121 ttgtttattt ttctaaatac attcaaatat gtatccgcta atgagacaat aaccctgata
     3181 aatgcttcaa ttatattgaa aaaggaagag tatgagtatc caacatttcc gtgtcgccct
     3241 tattcccttt tttgcggcct tctgccttcc tgtttttgct cacccagaaa cgctggtgaa
     3301 agtaaaagat gctgaagatc agttgggtgc ccgagtgggt tacattgaac tggatctcaa
     3361 cagcggcaaa atcttagaga gttttcgccc cgaagaacgc ttcccaatga tgagcacttt
     3421 caaagttctg ctatgtggcg cggtgttatc ccgtattgat gctgggcaag agcaactcgg
     3481 tcgccgcata cactattctc agaatgactt ggttgagtat tcaccagtca cagaaaagca
     3541 tcttacggat ggcatgacag taagagaatt atgtagtgct gccataacca tgagtgataa
     3601 cactgcggcc aacttacttc tgacaacgat tggaggaccg aaggagctaa ccgctttttt
     3661 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     3721 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa ctctccgcaa
     3781 actattaact ggcgaactac ttactcttgc ttcacgccag caactcattg actggatgga
     3841 ggcggacaaa gttgcaggac cacttctgcg ctcggcactt ccggctggct ggtttattgc
     3901 tgataaatct ggagccggtg agcgtggctc tcgcggtatc attgcagcac tggggccaga
     3961 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     4021 acgaaataga cagatcgctg agataggtgc ctca
//
