LOCUS       sequence-270387-        3827 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Module plasmid, individual component of Fungal Bioluminescent 
            Pathway, Npga.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3827)
  AUTHORS   Khakhar A, Starker CG, Chamness JC, Lee N, Stokke S, Wang C, Swanson
            R, Rizvi F, Imaizumi T, Voytas DF
  TITLE     Building customizable auto-luminescent luciferase-based reporters in
            plants.
  JOURNAL   Elife. 2020 Mar 25;9. pii: 52786. doi: 10.7554/eLife.52786.
  PUBMED    32209230
REFERENCE   2  (bases 1 to 3827)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2020-03-25"; volume: "9. pii: 52786. doi"; pages: " 
            10.7554/eLife.52786"
COMMENT     Sequence Label: pCO486
FEATURES             Location/Qualifiers
     source          1..3827
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     49..66
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        191..536
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     479..499
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     terminator      1687..1887
                     /label=gene 7 terminator
                     /note="transcription terminator for octopine-type Ti 
                     plasmid gene 7"
     promoter        1962..2066
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      3134..3722
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3623..3642
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
       61 agcgaggaag cggaagagcg cccacctgct catgatcggc gcgccctatg gaggtcaaca
      121 tggtggagca cgacactctg gtctactcca aaaatgtcaa agatacagtc tcagaagatc
      181 aaagggctat tgagactttt caacaaagga taatttcggg aaacctcctc ggattccatt
      241 gcccagctat ctgtcacttc atcgaaagga cagtagaaaa ggaaggtggc tcctacaaat
      301 gccatcattg cgataaagga aaggctatca ttcaagatct ctctgccgac agtggtccca
      361 aagatggacc cccacccacg aggagcatcg tggaaaaaga agaggttcca accacgtcta
      421 caaagcaagt ggattgatgt gacatctcca ctgacgtaag ggatgacgca caatcccact
      481 atccttcgca agacccttcc tctatataag gaagttcatt tcatttggag aggacacgct
      541 cgagtataag agctcatttt tacaacaatt accaacaaca acaaacaaca aacaacatta
      601 caattacatt tacaattatc gatacaatgg tgcaggacac ttcaagtgct agcacaagtc
      661 caattttaac cagatggtat atagacactc gacctctcac ggcaagtacc gccgcacttc
      721 cgctgctgga aacgctgcaa ccagctgatc agatctccgt ccaaaaatat tatcatctca
      781 aggacaagca catgtcactt gcctccaacc ttttgaagta tttattcgtt catcgaaatt
      841 gccgaatacc atggagtagt attgtcatat caagaacgcc cgacccgcac agaaggcctt
      901 gctatatacc tccttcaggt tcacaagagg actccttcaa agacggatat acgggaatca
      961 acgtagaatt caacgttagt caccaagcca gcatggtggc aatcgcaggt acggctttca
     1021 cacctaatag cggtggagat agcaaactca aaccggaggt cggaatagat ataacatgtg
     1081 taaatgagag gcagggcagg aatggagaag aaagatcctt ggaatccctg aggcagtata
     1141 tagatatatt cagcgaagta ttcagcaccg cagagatggc taatataagg cgacttgatg
     1201 gggtttcctc aagttccctt agtgcagaca gactcgtaga ttatggatac cgattattct
     1261 acacctattg ggccttaaaa gaggcttata tcaagatgac aggtgaggcc ttattggctc
     1321 cttggttgag agagctggag ttttccaacg tggtcgctcc agccgctgtc gccgaaagcg
     1381 gggactctgc tggagacttt ggcgaaccgt acactggcgt gcgtacaaca ttgtataaga
     1441 acctcgtaga ggacgtacga attgaggtag ctgctttggg aggagattat ctgttcgcaa
     1501 cagccgctag aggtggtggt ataggcgcat cctcacgtcc tggtggtggc ccagatggct
     1561 ctggaattag atcccaggac ccttggaggc cgttcaagaa actcgatata gagcgtgaca
     1621 tacagccatg tgctacgggc gtttgtaatt gcctgtcctc aggataggct tgatcccccg
     1681 tcgacagcta gctatatcat caatttatgt attacacata atatcgcact cagtctttca
     1741 tctacggcaa tgtaccagct gatataatca gttattgaaa tatttctgaa tttaaacttg
     1801 catcaataaa tttatgtttt tgcttggact ataatacctg acttgttatt ttatcaataa
     1861 atatttaaac tatatttctt tcaagatcgc tgttagagct cggacgtggg caggtgtgat
     1921 gaaaagcctt agggtcaggt ggcacttttc ggggaaatgt gcgatgaacc cctatttgtt
     1981 tatttttcta aatacattca aatatgtatc cgctaatgag acaataaccc tgataaatgc
     2041 ttcaattata ttgaaaaagg aagagtatga gtatccaaca tttccgtgtc gcccttattc
     2101 ccttttttgc ggccttctgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     2161 aagatgctga agatcagttg ggtgcccgag tgggttacat tgaactggat ctcaacagcg
     2221 gcaaaatctt agagagtttt cgccccgaag aacgcttccc aatgatgagc actttcaaag
     2281 ttctgctatg tggcgcggtg ttatcccgta ttgatgctgg gcaagagcaa ctcggtcgcc
     2341 gcatacacta ttctcagaat gacttggttg agtattcacc agtcacagaa aagcatctta
     2401 cggatggcat gacagtaaga gaattatgta gtgctgccat aaccatgagt gataacactg
     2461 cggccaactt acttctgaca acgattggag gaccgaagga gctaaccgct tttttgcaca
     2521 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     2581 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaactctc cgcaaactat
     2641 taactggcga actacttact cttgcttcac gccagcaact cattgactgg atggaggcgg
     2701 acaaagttgc aggaccactt ctgcgctcgg cacttccggc tggctggttt attgctgata
     2761 aatctggagc cggtgagcgt ggctctcgcg gtatcattgc agcactgggg ccagatggta
     2821 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     2881 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagacgaag
     2941 ggaaataaat agtagcccgc ctgatgtgcg ggcttttttt tggtggtggt taccagtgtc
     3001 gcttagctac cgtaggtcct cttaggcggt ccgattagcg acatagtcat caatgccttg
     3061 acggcaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     3121 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     3181 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctactaact ctttttccga
     3241 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     3301 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     3361 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     3421 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgctca cagcccagct
     3481 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     3541 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     3601 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     3661 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     3721 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     3781 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattacc
//
