LOCUS       sequence-270388-        4504 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Module plasmid, individual component of Fungal Bioluminescent 
            Pathway, H3H.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4504)
  AUTHORS   Khakhar A, Starker CG, Chamness JC, Lee N, Stokke S, Wang C, Swanson
            R, Rizvi F, Imaizumi T, Voytas DF
  TITLE     Building customizable auto-luminescent luciferase-based reporters in
            plants.
  JOURNAL   Elife. 2020 Mar 25;9. pii: 52786. doi: 10.7554/eLife.52786.
  PUBMED    32209230
REFERENCE   2  (bases 1 to 4504)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2020-03-25"; volume: "9. pii: 52786. doi"; pages: " 
            10.7554/eLife.52786"
COMMENT     Sequence Label: pCO487
FEATURES             Location/Qualifiers
     source          1..4504
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     49..66
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      1856..2563
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     promoter        2639..2743
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      3811..4399
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4300..4319
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
       61 agcgaggaag cgggagagcg cccacctgcc aggggacggc gcgccctatg gactggcaga
      121 catactgtcc cacaaatgaa gatggaatct gtaaaagaaa acgcgtgaaa taatgcgtct
      181 gacaaaggtt aggtcggctg cctttaatca ataccaaagt ggtccctacc acgatggaaa
      241 aactgtgcag tcggtttggc tttttctgac gaacaaataa gattcgtggc cgacaggtgg
      301 gggtccacca tgtgaaggca tcttcagact ccaataatgg agcaatgacg taagggctta
      361 cgaaataagt aagggtagtt tgggaaatgt ccactcaccc gtcagtctat aaatacttag
      421 cccctccctc attgttaagg gagcaaaatc tcagagagat agtcctagag agagaaagag
      481 agcaagtagc ctagaagtag tcaaggcggc gaagtattca ggcacgtggc caggaagaag
      541 aaaagccaag acgacgaaaa caggtaagag ctaagcttaa tggcatcctt tgaaaattca
      601 ctcagtgtct taatagttgg ggctggctta ggtggtttgg ccgctgctat agcactgcga
      661 aggcaaggac atgtagtcaa gatatacgat agttcttcat tcaaggccga attgggagca
      721 ggattagctg taccacccaa tactctccgt tccctgcaac aactgggctg taatacagaa
      781 aacctgaacg gagtagacaa cctctgcttt acggccatgg gatacgacgg gagtgtaggt
      841 atgatgaata atatgaccga ctaccgtgag gcatacggca cttcctggat aatggtacat
      901 cgagtggatc tccacaacga gctgatgcga gtcgccttgg atccgggcgg gttagggcct
      961 cctgccactc ttcaccttaa ccatcgtgtt acgttctgcg atgtggatgc ctgtacagtc
     1021 acgtttacga acgggacgac acagtctgct gacttgattg tcggagctga tggtattagg
     1081 tctacgattc gaagattcgt tctggaggag gacgtgactg ttccagctag cggaattgtc
     1141 ggctttcgat ggttggtcca ggccgatgct ctcgacccat atcccgaact ggattggatt
     1201 gtcaaaaaac cacccttggg agccaggttg atttctactc cacagaaccc gcagagtggc
     1261 gtaggactgg ccgatagacg aacgatcatt atttacgcat gcagaggtgg gaccatggtt
     1321 aatgtattag ccgtacacga cgacgaaaga gatcaaaaca ctgctgattg gtccgttccc
     1381 gcatcaaagg atgatttatt cagagtgttt cacgattacc atccaagatt ccgacgactc
     1441 ttagagttag cacaggacat taatttatgg cagatgaggg tggtcccggt cttgaaaaaa
     1501 tgggtgaaca agagggtatg cttgctgggt gatgccgccc atgcttcact gcccacactt
     1561 ggacaaggct tcgggatggg gttggaggat gcagtcgctt tgggcacgtt gttgcctaag
     1621 ggaacgaccg catcacagat cgagacaagg ctggccgttt acgaacagct gcgaaaggat
     1681 cgagctgagt ttgtcgccgc agagagctat gaggagcaat atgtcccaga gatgagaggg
     1741 ctgtacctta ggtccaaaga attgagagac agagtgatgg gttatgatat taaggtagag
     1801 tcagagaagg ttttggaaac actgttgcga tcatccaact cagcttgagc ttgtcctgct
     1861 ttaatgagat atgcgagaag cctatgatcg catgatattt gctttcaatt ctgttgtgca
     1921 cgttgtaaaa aacctgagca tgtgtagctc agatccttac cgccggtttc ggttcattct
     1981 aatgaatata tcacccgtta ctatcgtatt tttatgaata atattctccg ttcaatttac
     2041 tgattgtacc ctactactta tatgtacaat attaaaatga aaacaatata ttgtgctgaa
     2101 taggtttata gcgacatcta tgatagagcg ccacaataac aaacaattgc gttttattat
     2161 tacaaatcca attttaaaaa aagcggcaga accggtcaaa cctaaaagac tgattacata
     2221 aatcttattc aaatttcaaa agtgccccag gggctagtat ctacgacaca ccgagcggcg
     2281 aactaataac gctcactgaa gggaactccg gttccccgcc ggcgcgcatg ggtgagattc
     2341 cttgaagttg agtattggcc gtccgctcta ccgaaagtta cgggcaccat tcaacccggt
     2401 ccagcacggc ggccgggtaa ccgacttgct gccccgagaa ttatgcagca tttttttggt
     2461 gtatgtgggc cccaaatgaa gtgcaggtca aaccttgaca gtgacgacaa atcgttgggc
     2521 gggtccaggg cgaattttgc gacaacatgt cgaggctcag caggaccgct gttagagctc
     2581 ccggtgacgc aggtgatgaa aagccttagg gtcaggtggc acttttcggg gaaatgtgcg
     2641 atgaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc taatgagaca
     2701 ataaccctga taaatgcttc aattatattg aaaaaggaag agtatgagta tccaacattt
     2761 ccgtgtcgcc cttattccct tttttgcggc cttctgcctt cctgtttttg ctcacccaga
     2821 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcccgagtgg gttacattga
     2881 actggatctc aacagcggca aaatcttaga gagttttcgc cccgaagaac gcttcccaat
     2941 gatgagcact ttcaaagttc tgctatgtgg cgcggtgtta tcccgtattg atgctgggca
     3001 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt attcaccagt
     3061 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgtagtg ctgccataac
     3121 catgagtgat aacactgcgg ccaacttact tctgacaacg attggaggac cgaaggagct
     3181 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     3241 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     3301 aactctccgc aaactattaa ctggcgaact acttactctt gcttcacgcc agcaactcat
     3361 tgactggatg gaggcggaca aagttgcagg accacttctg cgctcggcac ttccggctgg
     3421 ctggtttatt gctgataaat ctggagccgg tgagcgtggc tctcgcggta tcattgcagc
     3481 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     3541 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     3601 gtaactgtca gacgaaggga aataaatagt agcccgcctg atgtgcgggc ttttttttgg
     3661 tggtggttac cagtgtcgct tagctaccgt aggtcctctt aggcggtccg attagcgaca
     3721 tagtcatcaa tgccttgacg gcaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     3781 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     3841 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     3901 actaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     3961 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     4021 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     4081 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     4141 gtgctcacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     4201 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     4261 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     4321 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     4381 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     4441 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     4501 tacc
//
