LOCUS       sequence-270391-        7854 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Module plasmid, individual component of Fungal Bioluminescent 
            Pathway, Hisps.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7854)
  AUTHORS   Khakhar A, Starker CG, Chamness JC, Lee N, Stokke S, Wang C, Swanson
            R, Rizvi F, Imaizumi T, Voytas DF
  TITLE     Building customizable auto-luminescent luciferase-based reporters in
            plants.
  JOURNAL   Elife. 2020 Mar 25;9. pii: 52786. doi: 10.7554/eLife.52786.
  PUBMED    32209230
REFERENCE   2  (bases 1 to 7854)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2020-03-25"; volume: "9. pii: 52786. doi"; pages: " 
            10.7554/eLife.52786"
COMMENT     Sequence Label: pCO601
FEATURES             Location/Qualifiers
     source          1..7854
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        109..454
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     397..417
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     misc_feature    473..528
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     polyA_signal    5661..5835
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        5907..6011
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      7079..7667
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7568..7587
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7821..7838
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cacctgcaac gccggggcgc gccctatgga ggtcaacatg gtggagcacg acactctggt
       61 ctactccaaa aatgtcaaag atacagtctc agaagatcaa agggctattg agacttttca
      121 acaaaggata atttcgggaa acctcctcgg attccattgc ccagctatct gtcacttcat
      181 cgaaaggaca gtagaaaagg aaggtggctc ctacaaatgc catcattgcg ataaaggaaa
      241 ggctatcatt caagatctct ctgccgacag tggtcccaaa gatggacccc cacccacgag
      301 gagcatcgtg gaaaaagaag aggttccaac cacgtctaca aagcaagtgg attgatgtga
      361 catctccact gacgtaaggg atgacgcaca atcccactat ccttcgcaag acccttcctc
      421 tatataagga agttcatttc atttggagag gacacgctac tgtattttta caacaattac
      481 caacaacaac aaacaacaaa caacattaca attactattt acaattacaa tgaatagtag
      541 taaaaaccct ccatccactc tgttagatgt ttttctggat accgctagga accttgatac
      601 agcatccaga aatgtcctgg agtgcggaga acacaggtgg agttacagag agttggacac
      661 agtgtcatcc gcattggccc aacaccttcg atacactgtg ggtcttagcc ccactgtagc
      721 cgtgattagc gagaatcacc cctacatact tgcattaatg ctcgccgtat ggaaactcgg
      781 cggaacgttc gcaccgatag atgttcattc cccagccgaa cttgttgctg gaatgttgaa
      841 tattgtaagc ccgtcatgtc ttgtcattcc tagctctgat gtgacaaatc agacattggc
      901 atgcgatctt aatataccgg ttgttgcatt ccacccacat caaagtacaa ttcccgaact
      961 caacaagaag tatctcacag attctcagat cagtcctgac ctgcccttcc ccgaccctaa
     1021 tcgacctgct ttgtacttat tcacgtcatc tgcaacgtct aggtccaatt tgaagtgcgt
     1081 gccacttaca cacacattca tattgcgaaa ctccttaagt aaacgagcat ggtgcaaaag
     1141 aatgaggcca gagacagatt ttgatggtat tagggtcctg gggtgggccc cctggagcca
     1201 tgtcctcgct catatgcagg acattggccc tcttacatta ttgaatgccg ggtgctacgt
     1261 atttgccacg accccatcta cctacccgac ggagctgaag gatgaccgtg atgttattag
     1321 ttgcgccgca aatgccgtaa tgtacaaagg tgttaaatcc ttcgcatgcc tcccattcgt
     1381 gcttggggga ctcaaggctt tatgtgagtc agagcccagt gttaaggcac aactgcaagt
     1441 ggaggaacga gcccagcttt tgaagtcact ccaacacatg aaaatcctgg aatgcggggg
     1501 ggcaatgctc gaagtgagtg tggcaagctg ggctatagag aataggattc ctatatctat
     1561 tgggattggt atgacagaga caggcggcgc tcttttcgct gggccggtcc aagcaattca
     1621 aaccggcttt agcagtgaag ataaatttat cgaggacgca acctacttgc tggtcaagga
     1681 cgattacgag agccacgcag aggaggacat aaacgagggc gagttagtgg taaagtccag
     1741 aatgttacct aggggttatt tgggctataa cgacccctct ttctctgttg acgatgctgg
     1801 ttgggttact ttcaaaactg gagatcgata ttcagtcact cctgacggca aattctcctg
     1861 gcttggtcga aatactgatt tcattcagat gacgagtggt gagactctcg atccccgtcc
     1921 gattgagtcc ctcctttgtg agtccagcct tatatcacga gcctgtgtca tcggggataa
     1981 gttcctgaac ggaccagcca cagctgtatg cgctattata gaactcgaac caacgactgt
     2041 tgagaaaggc caggctcaca gcagggatat tgcccgaatc tttgccccca tcaatcgaga
     2101 tcttcctccc ccgttacgta tagcatggag tcacgtactt gtacttcagc caagcgagaa
     2161 aataccgatg acgaaaaagg gtacaatctt ccgtaagaaa atcgaacagg ttttcggttc
     2221 cgctttagga ggcagtagcg gcgacaactc acaagcaacc actgacgctt cagtagttag
     2281 gagggatgag ttgagcaaca cggtcaaaca tatcataagc agagttttgg gagtctccga
     2341 cgatgaactc ctttggacac tgagctttgc tgagcttggc atgacgtccg ctcttgctac
     2401 tagaatagca aatgaattga acgaagttct ggtaggtgtg aacctcccca tcaatgcatg
     2461 ctacattcat gtggacctgc cttctttgtc aaatgccgtg tatgcaaagt tagcacacct
     2521 taaactcccg gataggacgc ccgagccaag gaaggcccca gttgaaaatc ccggtggaaa
     2581 ggaaatcgta atagtaggtc aggcttttag gcttccgggg agtataaatg atgtcgcttc
     2641 tttgagggac gcttttttgg ctagacaagc atccagtatt attactgaaa taccgccaga
     2701 tagatgggac cacgcatcat tttatccaaa agacatacga tttaataaag cagggctcgt
     2761 cgacatcgct aactacgacc acagtttttt cggattgaca gccacggagg cactgtacct
     2821 gtcaccaacg atgcgtttgg cacttgaagt gtcctttgaa gccttggaga atgctaatat
     2881 acccgtgtcc cagttaaagg gctctcaaac agctgtatat gtcgcaacca ccgacgacgg
     2941 gtttgagaca ctgctgaatg ctgaggcagg atatgatgca tacacacgat tctatggcac
     3001 tggtagggcc gcctctacgg ccagtggacg aattagttat ttactcgacg tacacggccc
     3061 ttcaattacc gtagacactg cttgttcagg cggtgctgtt tgcatagacc aggccataga
     3121 ctacctccaa tcctctagcg cagctgatac cgccataatc tgcgccagca acactcactg
     3181 ctggccaggt tccttcatgt ttctgagtgc tcaaggtatg gtaagttccg gggggcgatg
     3241 tgcaactttt acgacggatg ccgacggcta tgtaccctct gaaggtgctg tggcatttat
     3301 cctcaaaacc cgagaagcag ctatgagaga taaagatact atcttggcaa ctatcaaagc
     3361 aacgcagatt agtcataatg gtcgttccca aggtttagtc gcaccaaacg tcaattcaca
     3421 agctgattta cacagaagct tattgcagaa agctggtttg agtccagccg acattcactt
     3481 catcgaagct cacgggacag gaacgtcact cggtgacctt agtgaaatac aagcaattaa
     3541 tgatgcctac acgtcatctc agcctcgaac agccggaccc ttaatagtaa gcgcaagtaa
     3601 aaccgtcatt ggtcacacag agcctgctgg accgttggta ggcatgctct cagttctcaa
     3661 ctcctttaaa gagggcgcag ttcccggctt agctcacctg acagcagata acctcaatcc
     3721 ggctttagac tgctcctccg tccctttact tatcccctac cagcctgtcc acttggcagc
     3781 tcctaagcca cacagggctg ctgtgatgag ttatggcttc tccggcactc ttggaggtat
     3841 agttcttgaa gcaccggatg aggaaaggct tgaggaagaa ccacccaacg ataagcctat
     3901 gttatttgtt gttagcgcca aaactcatac tgcattgatt gagtaccttg gacgatacct
     3961 tgaatttctg ctccaggcca atccccaaga cttctgcgat atctgttaca ctagctgcgt
     4021 tggacgtgag cattacaggt accgttttgc atgtgtggca aacgatatgg aagatttaat
     4081 tggccaactt cagaagaggc ttgggtctaa ggttccacct aaaccgtcct ataaaagagg
     4141 agccctcgct tttgctttca gtggacaagg gacccagttt cgtggaatgg caacagaatt
     4201 ggctaaggca tatagtggtt ttcgtaagat agtcagtgat cttgctaaaa gagcatcaga
     4261 actgtccggc catgcaatcg acaggtttct gcttgcatat gacattgggg ctgagaatgt
     4321 tgcaccggat agtgaagctg atcaaatctg catattcgtt taccaatgta gtgttctcag
     4381 atggctccaa acaatgggga ttcgtccgtc agccgtgatc ggtcacagcc tcggtgagat
     4441 tagcgcatcc gtagctgctg gagcattgtc cttggactct gcccttgacc tcgtaataag
     4501 tagagccagg ttactgaggt catctacgaa tgccccagct ggcatggcag ctatgtctgc
     4561 atcccaggat gaagtagtcg aactcatagg aaagctcgat ttagataaag ctaatagcct
     4621 cagcgtgtcc gtaataaacg gcccccagaa caccgttgtt tcaggatctt ctgcagcaat
     4681 agaaagtatc gttgctctcg caaagggacg aaaaatcaag gccagcgcac tcaacattaa
     4741 ccaggcattc catagtcctt acgtcgattc tgcagtaccc ggcttacgag cttggagtga
     4801 gaaacatata tcatcagctc gaccattgca aatccctttg tactctaccc tcttgggcgc
     4861 tcaagtttct gagggtcaaa tgttaaatcc cgatcactgg gtagaccatg ctaggaaacc
     4921 cgttcagttc gcacaagcag ccactataat gaaggaatct ttcaccggag taataatcga
     4981 tattggacct caagttgtag catggtccct gctcttaagc aatggtctta cttccgtgac
     5041 cgcactcgcc gcaaagcgtg gccgttcaca gcaagtggcc ttcctctccg ccttagctga
     5101 tctttatcag gactacggcg tcgtaccaga ctttgttgga ctgtacgcac aacaggagga
     5161 cgcttccaga ctgaaaaaga ccgatatctt aacttacccc tttcagaggg ttcgacgtta
     5221 cccttccttt ataccctcta gaagggcacc cacccacgct catgtccaag acgaggaaac
     5281 tctgtcatca ggatcatcaa ctccaacctt agaaaatact gacctggaca gtgggaagga
     5341 atctctgatg ggaccaacaa gagggctttt gcgagtcgat gatcttaggg actcaatagt
     5401 cagttcagtc aaagatgtcc tggaattgaa gagtaacgaa gatctggacc tcagcgagtc
     5461 ccttaatgct cttggcatgg actctataat gttcgctcaa ttacgaaaaa ggatagggga
     5521 gggcctcggc ctgtccgttc caatggtttt tctcagcgat gccttcagta ttggagaaat
     5581 ggtcagtaac ctcgtggaac aggccgaagc ttccgaggac aattgagctt ctctagctag
     5641 agtcgatcga caagctcgag tttctccata ataatgtgtg agtagttccc agataaggga
     5701 attagggttc ctatagggtt tcgctcatgt gttgagcata taagaaaccc ttagtatgta
     5761 tttgtatttg taaaatactt ctatcaataa aatttctaat tcctaaaacc aaaatccagt
     5821 actaaaatcc agatcgctgt tagagctctc ccactagcag gtgatgaaaa gccttagggt
     5881 caggtggcac ttttcgggga aatgtgcgat gaacccctat ttgtttattt ttctaaatac
     5941 attcaaatat gtatccgcta atgagacaat aaccctgata aatgcttcaa ttatattgaa
     6001 aaaggaagag tatgagtatc caacatttcc gtgtcgccct tattcccttt tttgcggcct
     6061 tctgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     6121 agttgggtgc ccgagtgggt tacattgaac tggatctcaa cagcggcaaa atcttagaga
     6181 gttttcgccc cgaagaacgc ttcccaatga tgagcacttt caaagttctg ctatgtggcg
     6241 cggtgttatc ccgtattgat gctgggcaag agcaactcgg tcgccgcata cactattctc
     6301 agaatgactt ggttgagtat tcaccagtca cagaaaagca tcttacggat ggcatgacag
     6361 taagagaatt atgtagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     6421 tgacaacgat tggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     6481 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     6541 acaccacgat gcctgtagca atggcaacaa ctctccgcaa actattaact ggcgaactac
     6601 ttactcttgc ttcacgccag caactcattg actggatgga ggcggacaaa gttgcaggac
     6661 cacttctgcg ctcggcactt ccggctggct ggtttattgc tgataaatct ggagccggtg
     6721 agcgtggctc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     6781 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     6841 agataggtgc ctcactgatt aagcattggt aactgtcaga cgaagggaaa taaatagtag
     6901 cccgcctgat gtgcgggctt ttttttggtg gtggttacca gtgtcgctta gctaccgtag
     6961 gtcctcttag gcggtccgat tagcgacata gtcatcaatg ccttgacggc aaaatccctt
     7021 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     7081 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     7141 cggtggtttg tttgccggat caagagctac taactctttt tccgaaggta actggcttca
     7201 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     7261 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     7321 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     7381 cgcagcggtc gggctgaacg gggggttcgt gctcacagcc cagcttggag cgaacgacct
     7441 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     7501 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     7561 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     7621 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     7681 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     7741 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     7801 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcc
//
