LOCUS       sequence-270392-        7345 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  individual component of Fungal Bioluminescent Pathway, MoClo level 0
            plasmid, NnHisps.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7345)
  AUTHORS   Khakhar A, Starker CG, Chamness JC, Lee N, Stokke S, Wang C, Swanson
            R, Rizvi F, Imaizumi T, Voytas DF
  TITLE     Building customizable auto-luminescent luciferase-based reporters in
            plants.
  JOURNAL   Elife. 2020 Mar 25;9. pii: 52786. doi: 10.7554/eLife.52786.
  PUBMED    32209230
REFERENCE   2  (bases 1 to 7345)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2020-03-25"; volume: "9. pii: 52786. doi"; pages: " 
            10.7554/eLife.52786"
COMMENT     Sequence Label: pCO600
FEATURES             Location/Qualifiers
     source          1..7345
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(5145..5163)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5231..5335
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5555..6346
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      6517..7105
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7006..7025
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7259..7276
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ggtctcaaat gaatagtagt aaaaaccctc catccactct gttagatgtt tttctggata
       61 ccgctaggaa ccttgataca gcatccagaa atgtcctgga gtgcggagaa cacaggtgga
      121 gttacagaga gttggacaca gtgtcatccg cattggccca acaccttcga tacactgtgg
      181 gtcttagccc cactgtagcc gtgattagcg agaatcaccc ctacatactt gcattaatgc
      241 tcgccgtatg gaaactcggc ggaacgttcg caccgataga tgttcattcc ccagccgaac
      301 ttgttgctgg aatgttgaat attgtaagcc cgtcatgtct tgtcattcct agctctgatg
      361 tgacaaatca gacattggca tgcgatctta atataccggt tgttgcattc cacccacatc
      421 aaagtacaat tcccgaactc aacaagaagt atctcacaga ttctcagatc agtcctgacc
      481 tgcccttccc cgaccctaat cgacctgctt tgtacttatt cacgtcatct gcaacgtcta
      541 ggtccaattt gaagtgcgtg ccacttacac acacattcat attgcgaaac tccttaagta
      601 aacgagcatg gtgcaaaaga atgaggccag agacagattt tgatggtatt agggtcctgg
      661 ggtgggcccc ctggagccat gtcctcgctc atatgcagga cattggccct cttacattat
      721 tgaatgccgg gtgctacgta tttgccacga ccccatctac ctacccgacg gagctgaagg
      781 atgaccgtga tgttattagt tgcgccgcaa atgccgtaat gtacaaaggt gttaaatcct
      841 tcgcatgcct cccattcgtg cttgggggac tcaaggcttt atgtgagtca gagcccagtg
      901 ttaaggcaca actgcaagtg gaggaacgag cccagctttt gaagtcactc caacacatga
      961 aaatcctgga atgcgggggg gcaatgctcg aagtgagtgt ggcaagctgg gctatagaga
     1021 ataggattcc tatatctatt gggattggta tgacagagac aggcggcgct cttttcgctg
     1081 ggccggtcca agcaattcaa accggcttta gcagtgaaga taaatttatc gaggacgcaa
     1141 cctacttgct ggtcaaggac gattacgaga gccacgcaga ggaggacata aacgagggcg
     1201 agttagtggt aaagtccaga atgttaccta ggggttattt gggctataac gacccctctt
     1261 tctctgttga cgatgctggt tgggttactt tcaaaactgg agatcgatat tcagtcactc
     1321 ctgacggcaa attctcctgg cttggtcgaa atactgattt cattcagatg acgagtggtg
     1381 agactctcga tccccgtccg attgagtccc tcctttgtga gtccagcctt atatcacgag
     1441 cctgtgtcat cggggataag ttcctgaacg gaccagccac agctgtatgc gctattatag
     1501 aactcgaacc aacgactgtt gagaaaggcc aggctcacag cagggatatt gcccgaatct
     1561 ttgcccccat caatcgagat cttcctcccc cgttacgtat agcatggagt cacgtacttg
     1621 tacttcagcc aagcgagaaa ataccgatga cgaaaaaggg tacaatcttc cgtaagaaaa
     1681 tcgaacaggt tttcggttcc gctttaggag gcagtagcgg cgacaactca caagcaacca
     1741 ctgacgcttc agtagttagg agggatgagt tgagcaacac ggtcaaacat atcataagca
     1801 gagttttggg agtctccgac gatgaactcc tttggacact gagctttgct gagcttggca
     1861 tgacgtccgc tcttgctact agaatagcaa atgaattgaa cgaagttctg gtaggtgtga
     1921 acctccccat caatgcatgc tacattcatg tggacctgcc ttctttgtca aatgccgtgt
     1981 atgcaaagtt agcacacctt aaactcccgg ataggacgcc cgagccaagg aaggccccag
     2041 ttgaaaatcc cggtggaaag gaaatcgtaa tagtaggtca ggcttttagg cttccgggga
     2101 gtataaatga tgtcgcttct ttgagggacg cttttttggc tagacaagca tccagtatta
     2161 ttactgaaat accgccagat agatgggacc acgcatcatt ttatccaaaa gacatacgat
     2221 ttaataaagc agggctcgtc gacatcgcta actacgacca cagttttttc ggattgacag
     2281 ccacggaggc actgtacctg tcaccaacga tgcgtttggc acttgaagtg tcctttgaag
     2341 ccttggagaa tgctaatata cccgtgtccc agttaaaggg ctctcaaaca gctgtatatg
     2401 tcgcaaccac cgacgacggg tttgagacac tgctgaatgc tgaggcagga tatgatgcat
     2461 acacacgatt ctatggcact ggtagggccg cctctacggc cagtggacga attagttatt
     2521 tactcgacgt acacggccct tcaattaccg tagacactgc ttgttcaggc ggtgctgttt
     2581 gcatagacca ggccatagac tacctccaat cctctagcgc agctgatacc gccataatct
     2641 gcgccagcaa cactcactgc tggccaggtt ccttcatgtt tctgagtgct caaggtatgg
     2701 taagttccgg ggggcgatgt gcaactttta cgacggatgc cgacggctat gtaccctctg
     2761 aaggtgctgt ggcatttatc ctcaaaaccc gagaagcagc tatgagagat aaagatacta
     2821 tcttggcaac tatcaaagca acgcagatta gtcataatgg tcgttcccaa ggtttagtcg
     2881 caccaaacgt caattcacaa gctgatttac acagaagctt attgcagaaa gctggtttga
     2941 gtccagccga cattcacttc atcgaagctc acgggacagg aacgtcactc ggtgacctta
     3001 gtgaaataca agcaattaat gatgcctaca cgtcatctca gcctcgaaca gccggaccct
     3061 taatagtaag cgcaagtaaa accgtcattg gtcacacaga gcctgctgga ccgttggtag
     3121 gcatgctctc agttctcaac tcctttaaag agggcgcagt tcccggctta gctcacctga
     3181 cagcagataa cctcaatccg gctttagact gctcctccgt ccctttactt atcccctacc
     3241 agcctgtcca cttggcagct cctaagccac acagggctgc tgtgatgagt tatggcttct
     3301 ccggcactct tggaggtata gttcttgaag caccggatga ggaaaggctt gaggaagaac
     3361 cacccaacga taagcctatg ttatttgttg ttagcgccaa aactcatact gcattgattg
     3421 agtaccttgg acgatacctt gaatttctgc tccaggccaa tccccaagac ttctgcgata
     3481 tctgttacac tagctgcgtt ggacgtgagc attacaggta ccgttttgca tgtgtggcaa
     3541 acgatatgga agatttaatt ggccaacttc agaagaggct tgggtctaag gttccaccta
     3601 aaccgtccta taaaagagga gccctcgctt ttgctttcag tggacaaggg acccagtttc
     3661 gtggaatggc aacagaattg gctaaggcat atagtggttt tcgtaagata gtcagtgatc
     3721 ttgctaaaag agcatcagaa ctgtccggcc atgcaatcga caggtttctg cttgcatatg
     3781 acattggggc tgagaatgtt gcaccggata gtgaagctga tcaaatctgc atattcgttt
     3841 accaatgtag tgttctcaga tggctccaaa caatggggat tcgtccgtca gccgtgatcg
     3901 gtcacagcct cggtgagatt agcgcatccg tagctgctgg agcattgtcc ttggactctg
     3961 cccttgacct cgtaataagt agagccaggt tactgaggtc atctacgaat gccccagctg
     4021 gcatggcagc tatgtctgca tcccaggatg aagtagtcga actcatagga aagctcgatt
     4081 tagataaagc taatagcctc agcgtgtccg taataaacgg cccccagaac accgttgttt
     4141 caggatcttc tgcagcaata gaaagtatcg ttgctctcgc aaagggacga aaaatcaagg
     4201 ccagcgcact caacattaac caggcattcc atagtcctta cgtcgattct gcagtacccg
     4261 gcttacgagc ttggagtgag aaacatatat catcagctcg accattgcaa atccctttgt
     4321 actctaccct cttgggcgct caagtttctg agggtcaaat gttaaatccc gatcactggg
     4381 tagaccatgc taggaaaccc gttcagttcg cacaagcagc cactataatg aaggaatctt
     4441 tcaccggagt aataatcgat attggacctc aagttgtagc atggtccctg ctcttaagca
     4501 atggtcttac ttccgtgacc gcactcgccg caaagcgtgg ccgttcacag caagtggcct
     4561 tcctctccgc cttagctgat ctttatcagg actacggcgt cgtaccagac tttgttggac
     4621 tgtacgcaca acaggaggac gcttccagac tgaaaaagac cgatatctta acttacccct
     4681 ttcagagggt tcgacgttac ccttccttta taccctctag aagggcaccc acccacgctc
     4741 atgtccaaga cgaggaaact ctgtcatcag gatcatcaac tccaacctta gaaaatactg
     4801 acctggacag tgggaaggaa tctctgatgg gaccaacaag agggcttttg cgagtcgatg
     4861 atcttaggga ctcaatagtc agttcagtca aagatgtcct ggaattgaag agtaacgaag
     4921 atctggacct cagcgagtcc cttaatgctc ttggcatgga ctctataatg ttcgctcaat
     4981 tacgaaaaag gataggggag ggcctcggcc tgtccgttcc aatggttttt ctcagcgatg
     5041 ccttcagtat tggagaaatg gtcagtaacc tcgtggaaca ggccgaagct tccgaggaca
     5101 attgagcttt gagaccacga agtggctctt cagtggacga aagggcctcg tgatacgcct
     5161 atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg
     5221 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     5281 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgcg
     5341 ctcacgcaac tggtccagaa ccttgaccga acgcagcggt ggtaacggcg cagtggcggt
     5401 tttcatggct tgttatgact gtttttttgg ggtacagtct atgcctcggg catccaagca
     5461 gcaagcgcgt tacgccgtgg gtcgatgttt gatgttatgg agcagcaacg atgttacgca
     5521 gcagggcagt cgccctaaaa caaagttaaa catcatgagg gaagcggtga tcgccgaagt
     5581 atcgactcaa ctatcagagg tagttggcgt catcgagcgc catctcgaac cgacgttgct
     5641 ggccgtacat ttgtacggct ccgcagtgga tggcggcctg aagccacaca gcgatattga
     5701 tttgctggtt acggtgaccg taaggcttga tgaaacaacg cggcgagctt tgatcaacga
     5761 ccttttggaa acttcggctt cccctggaga gagcgagatt ctccgcgctg tagaagtcac
     5821 cattgttgtg cacgacgaca tcattccgtg gcgttatcca gctaagcgcg aactgcaatt
     5881 tggagaatgg cagcgcaatg acattcttgc aggtatcttc gagccagcca cgatcgacat
     5941 tgatctggct atcttgctga caaaagcaag agaacatagc gttgccttgg taggtccagc
     6001 ggcggaggaa ctctttgatc cggttcctga acaggatcta tttgaggcgc taaatgaaac
     6061 cttaacgcta tggaactcgc cgcccgactg ggctggcgat gagcgaaatg tagtgcttac
     6121 gttgtcccgc atttggtaca gcgcagtaac cggcaaaatc gcgccgaagg atgtcgctgc
     6181 cgactgggca atggagcgcc tgccggccca gtatcagccc gtcatacttg aagctagaca
     6241 ggcttatctt ggacaagaag aagatcgctt ggcctcgcgc gcagatcagt tggaagaatt
     6301 tgtccattac gtaaaaggcg agatcaccaa ggtagtcggc aaataactgt cagaccaagt
     6361 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     6421 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     6481 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     6541 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     6601 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     6661 tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     6721 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     6781 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     6841 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     6901 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     6961 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     7021 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     7081 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     7141 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     7201 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
     7261 cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg
     7321 ttggccgatt cattaatcac tctgt
//
