LOCUS       sequence-270409-       10655 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of human ECT2 WT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10655)
  AUTHORS   de Carcer G, Wachowicz P, Martinez-Martinez S, Oller J, 
            Mendez-Barbero N, Escobar B, Gonzalez-Loyola A, Takaki T, El Bakkali
            A, Camara JA, Jimenez-Borreguero LJ, Bustelo XR, Canamero M, Mulero 
            F, de Los Angeles Sevilla M, Montero MJ, Redondo JM, Malumbres M
  TITLE     Plk1 regulates contraction of postmitotic smooth muscle cells and is
            required for vascular homeostasis.
  JOURNAL   Nat Med. 2017 Aug;23(8):964-974. doi: 10.1038/nm.4364. Epub 2017 Jul
            10.
  PUBMED    28692064
REFERENCE   2  (bases 1 to 10655)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nm.4364"; 
            journalName: "Nat Med"; date: "2017-08"; volume: "23"; issue: "8"; 
            pages: "964-974"
COMMENT     Sequence Label: pLENTI_Hu_ECT2(WT)
FEATURES             Location/Qualifiers
     source          1..10655
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     95..112
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    229..250
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(265..270,271..288,289..295)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    303..319
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     308..330
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     327..343
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     327..343
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     362..382
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        364..382
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        410..636
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             637..817
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    864..989
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1482..1715
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1900..1944
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2211..2327
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2350..2653
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2654..2857
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2808..2828
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2854..2878
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    2962..2986
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5669..5693)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    5724..6312
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5777..5797)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6195..6206)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        6326..6825
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(6361..6384)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6758..6777
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             6846..7445
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6846..6865)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7342..7362
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             7583..7816
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7888..8009
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7925..7944)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7979..7998
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8049..8184
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8204..8223)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8205..8223)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8233..8250)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8233..8249)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8242..8264)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8391..8846
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8478..8497)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8688..8709
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8872..8976
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8977..9045,9046..9837)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9195..9214)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10008..10596
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10497..10516
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
       61 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
      121 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
      181 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta
      241 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg
      301 aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagcg
      361 cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat gtagtcttat
      421 gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc cttacaagga
      481 gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg tgccttatta
      541 ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc gcattgcaga
      601 gatattgtat ttaagtgcct agctcgatac ataaacgggt ctctctggtt agaccagatc
      661 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
      721 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
      781 ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga
      841 aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca
      901 cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct
      961 agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat
     1021 gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat
     1081 gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag
     1141 gctgtagaca aatactggga cagctacaac catcccttca gacaggatca gaagaactta
     1201 gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag
     1261 acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac
     1321 agcaagcggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt
     1381 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca
     1441 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg
     1501 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc
     1561 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg
     1621 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg
     1681 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
     1741 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
     1801 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
     1861 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
     1921 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
     1981 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
     2041 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
     2101 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac
     2161 agagacagat ccattcgatt agtgaacgga tctcgacggt atcggttaac ttttaaaaga
     2221 aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac
     2281 atacaaacta aagaattaca aaaacaaatt acaaaattca aaattttatc gataagcttg
     2341 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
     2401 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
     2461 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
     2521 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
     2581 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
     2641 cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga
     2701 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
     2761 aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg
     2821 taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc gtcagatcgc
     2881 ctggagacgc catccacgct gttttgacct ccatagaaga caccgactct agtccagtgt
     2941 ggtggaattc tgcagatatc aacaagtttg tacaaaaaag caggctccgc ggccgccccc
     3001 ttcaccatgg ctgaaaatag tgtattaaca tccactactg ggaggactag cttggcagac
     3061 tcttccattt ttgattctaa agttactgag atttccaagg aaaacttact tattggatct
     3121 acttcatatg tagaagagat gcctcagatt gaaacaagag tgatattggt tcaagaagct
     3181 ggaaaacaag aagaacttat aaaagcctta aaggacatta aagtgggctt tgtaaagatg
     3241 gagtcagtgg aagaatttga aggtttggat tctccggaat ttgaaaatgt atttgtagtc
     3301 acggactttc aggattctgt ctttaatgac ctctacaagg ctgattgtag agttattgga
     3361 ccacccgtgg tgctgaattg ttcacaaaaa ggagagcctt tgccattttc atgtcgcccg
     3421 ttgtattgta caagtatgat gaatctagta ctatgcttta ctggatttag gaaaaaagaa
     3481 gaactagtca ggttggtgac attggtccat cacatgggtg gagttattcg aaaagacttt
     3541 aattcaaaag ttacacattt ggtggcaaat tgtacacaag gagaaaaatt cagggttgct
     3601 gtgagtctag gtactccaat tatgaagcca gaatggattt ataaagcttg ggaaaggcgg
     3661 aatgaacagg atttctatgc agcagttgat gactttagaa atgaatttaa agttcctcca
     3721 tttcaagatt gtattttaag tttcctggga ttttcagatg aagagaaaac caatatggaa
     3781 gaaatgactg aaatgcaagg aggtaaatat ttaccgcttg gagatgaaag atgcactcac
     3841 cttgtagttg aagagaatat agtaaaagat cttccctttg aaccttcaaa gaaactttat
     3901 gttgtcaagc aagagtggtt ctggggaagc attcaaatgg atgcccgagc tggagaaact
     3961 atgtatttat atgaaaaggc aaatactcct gagctcaaga aatcagtgtc aatgctttct
     4021 ctaaataccc ctaacagcaa tcgcaaacga cgtcgtttaa aagaaacact tgctcagctt
     4081 tcaagagaga cagacgtgtc accatttcca ccccgtaagc gcccatcagc tgagcattcc
     4141 ctttccatag ggtcactcct agatatctcc aacacaccag agtctagcat taactatgga
     4201 gacaccccaa agtcttgtac taagtcttct aaaagctcca ctccagttcc ttcaaagcag
     4261 tcagcaaggt ggcaagttgc aaaagagctt tatcaaactg aaagtaatta tgttaatata
     4321 ttggcaacaa ttattcagtt atttcaagta ccattggaag aggaaggaca acgtggtgga
     4381 cctatccttg caccagagga gattaagact atttttggta gcatcccaga tatctttgat
     4441 gtacacacta agataaagga tgatcttgaa gaccttatag ttaattggga tgagagcaaa
     4501 agcattggtg acatttttct gaaatattca aaagatttgg taaaaaccta ccctcccttt
     4561 gtaaacttct ttgaaatgag caaggaaaca attattaaat gtgaaaaaca gaaaccaaga
     4621 tttcatgctt ttctcaagat aaaccaagca aaaccagaat gtggacggca gagccttgtt
     4681 gaacttctta tccgaccagt acagaggtta cccagtgttg cattactttt aaatgatctt
     4741 aagaagcata cagctgatga aaatccagac aaaagcactt tagaaaaagc tattggatca
     4801 ctgaaggaag taatgacgca tattaatgag gataagagaa aaacagaagc tcaaaagcaa
     4861 atttttgatg ttgtttatga agtagatgga tgcccagcta atcttttatc ttctcaccga
     4921 agcttagtac agcgggttga aacaatttct ctaggtgagc acccctgtga cagaggagaa
     4981 caagtaactc tcttcctctt caatgattgc ctagagatag caagaaaacg gcacaaggtt
     5041 attggcactt ttaggagtcc tcatggccaa acccgacccc cagcttctct taagcatatt
     5101 cacctaatgc ctctttctca gattaagaag gtattggaca taagagagac agaagattgc
     5161 cataatgctt ttgccttgct tgtgaggcca ccaacagagc aggcaaatgt gctactcagt
     5221 ttccagatga catcagatga acttccaaaa gaaaactggc taaagatgct gtgtcgacat
     5281 gtagctaaca ccatttgtaa agcagatgct gagaatctta tttatactgc tgatccagaa
     5341 tcctttgaag taaatacaaa agatatggac agtacattga gtagagcatc aagagcaata
     5401 aaaaagactt caaaaaaggt tacaagagca ttctctttct ccaaaactcc aaaaagagct
     5461 cttcgaaggg ctcttatgac atcccacggc tcagtggagg gaagaagtcc ttccagcaat
     5521 gataagcatg taatgagtcg tctttctagc acatcatcat tagcaggtat cccttctccc
     5581 tcccttgtca gccttccttc cttctttgaa aggagaagtc atacgttaag tagatctaca
     5641 actcatttga taaagggtgg gcgcgccgac ccagctttct tgtacaaagt ggttgatatc
     5701 cagcacagtg gcggccgctc gacaatcaac ctctggatta caaaatttgt gaaagattga
     5761 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt
     5821 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt
     5881 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg
     5941 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg
     6001 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc
     6061 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaagc
     6121 tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct
     6181 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg
     6241 ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg
     6301 ccgcctcccc gcctggaatt ctaccgggta ggggaggcgc ttttcccaag gcagtctgga
     6361 gcatgcgctt tagcagcccc gctgggcact tggcgctaca caagtggcct ctggcctcgc
     6421 acacattcca catccaccgg taggcgccaa ccggctccgt tctttggtgg ccccttcgcg
     6481 ccaccttcta ctcctcccct agtcaggaag ttcccccccg ccccgcagct cgcgtcgtgc
     6541 aggacgtgac aaatggaagt agcacgtctc actagtctcg tgcagatgga cagcaccgct
     6601 gagcaatgga agcgggtagg cctttggggc agcggccaat agcagctttg ctccttcgct
     6661 ttctgggctc agaggctggg aaggggtggg tccgggggcg ggctcagggg cgggctcagg
     6721 ggcggggcgg gcgcccgaag gtcctccgga ggcccggcat tctgcacgct tcaaaagcgc
     6781 acgtctgccg cgctgttctc ctcttcctca tctccgggcc tttcgacctg cagcccaagc
     6841 ttaccatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtccccaggg
     6901 ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgatc
     6961 cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc
     7021 tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc
     7081 cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga
     7141 gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca
     7201 aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc
     7261 tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct
     7321 tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca
     7381 ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg
     7441 cctgacgccc gccccacgac ccgcagcgcc cgaccgaaag gagcgcacga ccccatgcat
     7501 ctcgagggcc cggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac
     7561 tttttaaaag aaaagggggg actggaaggg ctagctcact cccaacgaag acaagatctg
     7621 ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc
     7681 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
     7741 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
     7801 tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca
     7861 aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa
     7921 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     7981 ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa
     8041 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     8101 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt
     8161 agtgaggagg cttttttgga ggcctaggga cgtacccaat tcgccctata gtgagtcgta
     8221 ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac
     8281 ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc
     8341 ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggg acgcgccctg
     8401 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
     8461 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     8521 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
     8581 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg
     8641 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
     8701 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt
     8761 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt
     8821 taacaaaata ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc
     8881 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     8941 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     9001 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     9061 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     9121 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     9181 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
     9241 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     9301 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     9361 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     9421 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     9481 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
     9541 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     9601 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     9661 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     9721 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     9781 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     9841 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     9901 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     9961 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
    10021 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
    10081 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
    10141 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
    10201 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
    10261 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
    10321 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
    10381 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
    10441 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
    10501 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
    10561 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
    10621 cggttcctgg ccttttgctg gccttttgct cacat
//
