LOCUS       sequence-270441-        8334 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GFP-tagged gene expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8334)
  AUTHORS   Yadav L, Pietila E, Ohman T, Liu X, Mahato AK, Sidorova Y, Lehti K, 
            Saarma M, Varjosalo M
  TITLE     PTPRA Phosphatase Regulates GDNF-Dependent RET Signaling and 
            Inhibits the RET Mutant MEN2A Oncogenic Potential.
  JOURNAL   iScience. 2020 Feb 21;23(2):100871. doi: 10.1016/j.isci.2020.100871.
            Epub 2020 Jan 31.
  PUBMED    32062451
REFERENCE   2  (bases 1 to 8334)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "iScience.";
            date: "2020-02-21"; volume: "23(2):100871. doi"; pages: " 
            10.1016/j.isci.2020.100871. Epub 2020 Jan 31"
COMMENT     Sequence Label: PTPRA_C723S-GFP fusion
FEATURES             Location/Qualifiers
     source          1..8334
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    820..838
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    841..859
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     869..893
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    1036..1060
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3449..3473)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      3481..3490
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3487..3489,3490..3492,3493..4203)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3487..3489,3490..3492,3493..4203)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3532..3553)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3793..3812)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4140..4161
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(4286..4303)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4292..4516
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(4649..4668)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    4800..4847
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             4855..5877
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             4855..5877
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    6007..6128
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6044..6063)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6098..6117
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6177..6193)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6177..6193)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6190..6212)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6201..6217
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6225..6231,6232..6249,6250..6255))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6270..6291
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6408..6425)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6579..7167)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6659..6678)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7338..8129,8130..8198))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7961..7980
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8199..8303)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ccctatcagt gatagagatc
      841 tccctatcag tgatagagat cgtcgacgag ctcgtttagt gaaccgtcag atcgcctgga
      901 gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgga
      961 ctctagcgtt taaacttaag cttggtaccg agctcggatc cactagtcca gtgtggtgga
     1021 attctgcaga tatcaacaag tttgtacaaa aaagcaggct ccaccatgga ttcctggttc
     1081 attcttgttc tgctcggcag tggtctgata tgtgtcagtg ccaacaatgc taccacagtt
     1141 gcaccttctg taggaattac aagattaatt aactcatcaa cggcagaacc agttaaagaa
     1201 gaggccaaaa cttcaaatcc aacttcttca ctaacttctc tttctgtggc accaacattc
     1261 agcccaaata taactctggg acccacctat ttaaccactg tcaattcttc agactctgac
     1321 aatgggacca caagaacagc aagcaccaat tctataggca ttacaatttc accaaatgga
     1381 acgtggcttc cagataacca gttcacggat gccagaacag aaccctggga ggggaattcc
     1441 agcaccgcag caaccactcc agaaactttc cctccttcag atgagacacc aattattgcg
     1501 gtgatggtgg ccctgtcctc tctgctagtg atcgtgttta ttatcatagt tttgtacatg
     1561 ttaaggttta agaaatacaa gcaagctggg agccattcca attctttccg cttatccaac
     1621 ggccgcactg aggatgtgga gccccagagt gtgccacttc tggccagatc cccaagcacc
     1681 aacaggaaat acccacccct gcccgtggac aagctggaag aggaaattaa ccggagaatg
     1741 gcagacgaca ataagctctt cagggaggaa ttcaacgctc tccctgcatg tcctatccag
     1801 gccacctgtg aggctgcttc caaggaggaa aacaaggaaa aaaatcgata tgtaaacatc
     1861 ttgccttatg accactctag agtccacctg acaccggttg aaggggttcc agattctgat
     1921 tacatcaatg cttcattcat caacggttac caagaaaaga acaaattcat tgctgcacaa
     1981 ggaccaaaag aagaaacggt gaatgatttc tggcggatga tctgggaaca aaacacagcc
     2041 accatcgtca tggttaccaa cctgaaggag agaaaggagt gcaagtgcgc ccagtactgg
     2101 ccagaccaag gctgctggac ctatgggaat attcgggtgt ctgtagagga tgtgactgtc
     2161 ctggtggact acacagtacg gaagttctgc atccagcagg tgggcgacat gaccaacaga
     2221 aagccacagc gcctcatcac tcagttccac tttaccagct ggccagactt tggggtgcct
     2281 tttaccccga tcggcatgct caagttcctc aagaaggtga aggcctgtaa ccctcagtat
     2341 gcaggggcca tcgtggtcca ctgcagtgca ggtgtagggc gtacaggtac ctttgtcgtc
     2401 attgatgcca tgctggacat gatgcataca gaacggaagg tggacgtgta tggctttgtg
     2461 agccggatcc gggcacagcg ctgccagatg gtgcaaaccg atatgcagta tgtcttcata
     2521 taccaagccc ttctggagca ttatctctat ggagatacag aactggaagt gacctctcta
     2581 gaaacccacc tgcagaaaat ttacaacaaa atcccaggga ccagcaacaa tggattagag
     2641 gaggagttta agaagttaac atcaatcaaa atccagaatg acaagatgcg gactggaaac
     2701 cttccagcca acatgaagaa gaaccgtgtt ttacagatca ttccatatga attcaacaga
     2761 gtgatcattc cagttaagcg gggcgaagag aatacagact atgtgaacgc atcctttatt
     2821 gatggctacc ggcagaagga ctcctatatc gccagccagg gccctcttct ccacacaatt
     2881 gaggacttct ggcgaatgat ctgggagtgg aaatcctgct ctatcgtgat gctaacagaa
     2941 ctggaggaga gaggccagga gaagtgtgcc cagtactggc catctgatgg actggtgtcc
     3001 tatggagata ttacagtgga actgaagaag gaggaggaat gtgagagcta caccgtccga
     3061 gacctcctgg tcaccaacac cagggagaat aagagccggc agatccggca gttccacttc
     3121 catggctggc ctgaagtggg catccccagt gacggaaagg gcatgatcag catcatcgcc
     3181 gccgtgcaga agcagcagca gcagtcaggg aaccacccca tcaccgtgca ctccagcgcc
     3241 ggggcaggaa ggacggggac cttctgtgcc ctgagcaccg tcctggagcg tgtgaaagca
     3301 gaggggattt tggatgtctt ccagactgtc aagagcctgc ggctacagag gccacacatg
     3361 gtccagacac tggaacagta tgagttctgc tacaaggtgg tgcaggagta tattgatgca
     3421 ttctcagatt atgccaactt caagttggac ccagctttct tgtacaaagt ggtgagctcc
     3481 gccaccatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag
     3541 ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc
     3601 acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg
     3661 cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac
     3721 atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc
     3781 atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac
     3841 accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg
     3901 gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag
     3961 aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag
     4021 ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac
     4081 aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac
     4141 atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac
     4201 aagcaccggt ccacgtgacg taagctagat ccagcacagt ggcggccgct cgagtctaga
     4261 gggcccgttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt
     4321 gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc
     4381 taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt
     4441 ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat
     4501 gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc
     4561 cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     4621 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     4681 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     4741 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtacctag
     4801 aagttcctat tccgaagttc ctattctcta gaaagtatag gaacttcctt ggccaaaaag
     4861 cctgaactca ccgcgacgtc tgtcgagaag tttctgatcg aaaagttcga cagcgtctcc
     4921 gacctgatgc agctctcgga gggcgaagaa tctcgtgctt tcagcttcga tgtaggaggg
     4981 cgtggatatg tcctgcgggt aaatagctgc gccgatggtt tctacaaaga tcgttatgtt
     5041 tatcggcact ttgcatcggc cgcgctcccg attccggaag tgcttgacat tggggaattc
     5101 agcgagagcc tgacctattg catctcccgc cgtgcacagg gtgtcacgtt gcaagacctg
     5161 cctgaaaccg aactgcccgc tgttctgcag ccggtcgcgg aggccatgga tgcgatcgct
     5221 gcggccgatc ttagccagac gagcgggttc ggcccattcg gaccgcaagg aatcggtcaa
     5281 tacactacat ggcgtgattt catatgcgcg attgctgatc cccatgtgta tcactggcaa
     5341 actgtgatgg acgacaccgt cagtgcgtcc gtcgcgcagg ctctcgatga gctgatgctt
     5401 tgggccgagg actgccccga agtccggcac ctcgtgcacg cggatttcgg ctccaacaat
     5461 gtcctgacgg acaatggccg cataacagcg gtcattgact ggagcgaggc gatgttcggg
     5521 gattcccaat acgaggtcgc caacatcttc ttctggaggc cgtggttggc ttgtatggag
     5581 cagcagacgc gctacttcga gcggaggcat ccggagcttg caggatcgcc gcggctccgg
     5641 gcgtatatgc tccgcattgg tcttgaccaa ctctatcaga gcttggttga cggcaatttc
     5701 gatgatgcag cttgggcgca gggtcgatgc gacgcaatcg tccgatccgg agccgggact
     5761 gtcgggcgta cacaaatcgc ccgcagaagc gcggccgtct ggaccgatgg ctgtgtagaa
     5821 gtactcgccg atagtggaaa ccgacgcccc agcactcgtc cgagggcaaa ggaatagcac
     5881 gtactacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt
     5941 ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc
     6001 caccccaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     6061 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     6121 gtatcttatc atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca
     6181 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     6241 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     6301 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     6361 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     6421 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     6481 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     6541 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     6601 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     6661 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     6721 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     6781 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     6841 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     6901 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     6961 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     7021 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     7081 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     7141 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     7201 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     7261 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     7321 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     7381 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     7441 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     7501 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     7561 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     7621 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     7681 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     7741 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     7801 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     7861 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     7921 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     7981 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     8041 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     8101 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     8161 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     8221 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     8281 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtc
//
