LOCUS       sequence-270448-        9543 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MAC-tagged gene expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9543)
  AUTHORS   Yadav L, Pietila E, Ohman T, Liu X, Mahato AK, Sidorova Y, Lehti K, 
            Saarma M, Varjosalo M
  TITLE     PTPRA Phosphatase Regulates GDNF-Dependent RET Signaling and 
            Inhibits the RET Mutant MEN2A Oncogenic Potential.
  JOURNAL   iScience. 2020 Feb 21;23(2):100871. doi: 10.1016/j.isci.2020.100871.
            Epub 2020 Jan 31.
  PUBMED    32062451
REFERENCE   2  (bases 1 to 9543)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "iScience.";
            date: "2020-02-21"; volume: "23(2):100871. doi"; pages: " 
            10.1016/j.isci.2020.100871. Epub 2020 Jan 31"
COMMENT     Sequence Label: MEN2A-MAC
FEATURES             Location/Qualifiers
     source          1..9543
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    820..838
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    841..859
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     869..893
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    1036..1060
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4280..4304)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4351..4374
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             4411..4434
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             4465..4491
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             4465..4491
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             join(4492..4839,4840..4842,4843..5451)
                     /codon_start=1
                     /product="promiscuous R118G mutant of the E. coli biotin 
                     protein ligase (Cronan, 2005)"
                     /label=BirA(R118G)
                     /note="also known as BirA* or BioID"
                     /translation="KDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDW
                     GVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSGD
                     ACIAEYQQAGRGGRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRKL
                     GADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQGW
                     ITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGD
                     KEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     primer_bind     complement(5495..5512)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5501..5725
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5858..5877)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    6009..6056
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             6064..7086
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             6064..7086
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    7216..7337
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7253..7272)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7307..7326
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7386..7402)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7386..7402)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7399..7421)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7410..7426
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7434..7440,7441..7458,7459..7464))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7479..7500
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7617..7634)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7788..8376)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7868..7887)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8547..9338,9339..9407))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9170..9189
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9408..9512)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ccctatcagt gatagagatc
      841 tccctatcag tgatagagat cgtcgacgag ctcgtttagt gaaccgtcag atcgcctgga
      901 gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgga
      961 ctctagcgtt taaacttaag cttggtaccg agctcggatc cactagtcca gtgtggtgga
     1021 attctgcaga tatcaacaag tttgtacaaa aaagttggca ccatggcgaa ggcgacgtcc
     1081 ggtgccgcgg ggctgcgtct gctgttgctg ctgctgctgc cgctgctagg caaagtggca
     1141 ttgggcctct acttctcgag ggatgcttac tgggagaagc tgtatgtgga ccaggcggcc
     1201 ggcacgccct tgctgtacgt ccatgccctg cgggacgccc ctgaggaggt gcccagcttc
     1261 cgcctgggcc agcatctcta cggcacgtac cgcacacggc tgcatgagaa caactggatc
     1321 tgcatccagg aggacaccgg cctcctctac cttaaccgga gcctggacca tagctcctgg
     1381 gagaagctca gtgtccgcaa ccgcggcttt cccctgctca ccgtctacct caaggtcttc
     1441 ctgtcaccca catcccttcg tgagggcgag tgccagtggc caggctgtgc ccgcgtatac
     1501 ttctccttct tcaacacctc ctttccagcc tgcagctccc tcaagccccg ggagctctgc
     1561 ttcccagaga caaggccctc cttccgcatt cgggagaacc gacccccagg caccttccac
     1621 cagttccgcc tgctgcctgt gcagttcttg tgccccaaca tcagcgtggc ctacaggctc
     1681 ctggagggtg agggtctgcc cttccgctgc gccccggaca gcctggaggt gagcacgcgc
     1741 tgggccctgg accgcgagca gcgggagaag tacgagctgg tggccgtgtg caccgtgcac
     1801 gccggcgcgc gcgaggaggt ggtgatggtg cccttcccgg tgaccgtgta cgacgaggac
     1861 gactcggcgc ccaccttccc cgcgggcgtc gacaccgcca gcgccgtggt ggagttcaag
     1921 cggaaggagg acaccgtggt ggccacgctg cgtgtcttcg atgcagacgt ggtacctgca
     1981 tcaggggagc tggtgaggcg gtacacaagc acgctgctcc ccggggacac ctgggcccag
     2041 cagaccttcc gggtggaaca ctggcccaac gagacctcgg tccaggccaa cggcagcttc
     2101 gtgcgggcga ccgtacatga ctataggctg gttctcaacc ggaacctctc catctcggag
     2161 aaccgcacca tgcagctggc ggtgctggtc aatgactcag acttccaggg cccaggagcg
     2221 ggcgtcctct tgctccactt caacgtgtcg gtgctgccgg tcagcctgca cctgcccagt
     2281 acctactccc tctccgtgag caggagggct cgccgatttg cccagatcgg gaaagtctgt
     2341 gtggaaaact gccaggcatt cagtggcatc aacgtccagt acaagctgca ttcctctggt
     2401 gccaactgca gcacgctagg ggtggtcacc tcagccgagg acacctcggg gatcctgttt
     2461 gtgaatgaca ccaaggccct gcggcggccc aagtgtgccg aacttcacta catggtggtg
     2521 gccaccgacc agcagacctc taggcaggcc caggcccagc tgcttgtaac agtggagggg
     2581 tcatatgtgg ccgaggaggc gggctgcccc ctgtcctgtg cagtcagcaa gagacggctg
     2641 gagtgtgagg agtgtggcgg cctgggctcc ccaacaggca ggtgtgagtg gaggcaagga
     2701 gatggcaaag ggatcaccag gaacttctcc acctgctctc ccagcaccaa gacctgcccc
     2761 gacggccact gcgatgttgt ggagacccaa gacatcaaca tttgccctca ggactgcctc
     2821 cggggcagca ttgttggggg acacgagcct ggggagcccc gggggattaa agctggctat
     2881 ggcacctgca actgcttccc tgaggaggag aagtgcttct gcgagcccga agacatccag
     2941 gatccactgt gcgacgagct gtggcgcacg gtgatcgcag ccgctgtcct cttctccttc
     3001 atcgtctcgg tgctgctgtc tgccttctgc atccactgct accacaagtt tgcccacaag
     3061 ccacccatct cctcagctga gatgaccttc cggaggcccg cccaggcctt cccggtcagc
     3121 tactcctctt ccagtgcccg ccggccctcg ctggactcca tggagaacca ggtctccgtg
     3181 gatgccttca agatcctgga ggatccaaag tgggaattcc ctcggaagaa cttggttctt
     3241 ggaaaaactc taggagaagg cgaatttgga aaagtggtca aggcaacggc cttccatctg
     3301 aaaggcagag cagggtacac cacggtggcc gtgaagatgc tgaaagagaa cgcctccccg
     3361 agtgagcttc gagacctgct gtcagagttc aacgtcctga agcaggtcaa ccacccacat
     3421 gtcatcaaat tgtatggggc ctgcagccag gatggcccgc tcctcctcat cgtggagtac
     3481 gccaaatacg gctccctgcg gggcttcctc cgcgagagcc gcaaagtggg gcctggctac
     3541 ctgggcagtg gaggcagccg caactccagc tccctggacc acccggatga gcgggccctc
     3601 accatgggcg acctcatctc atttgcctgg cagatctcac aggggatgca gtatctggcc
     3661 gagatgaagc tcgttcatcg ggacttggca gccagaaaca tcctggtagc tgaggggcgg
     3721 aagatgaaga tttcggattt cggcttgtcc cgagatgttt atgaagagga ttcgtacgtg
     3781 aagaggagcc agggtcggat tccagttaaa tggatggcaa ttgaatccct ttttgatcat
     3841 atctacacca cgcaaagtga tgtatggtct tttggtgtcc tgctgtggga gatcgtgacc
     3901 ctagggggaa acccctatcc tgggattcct cctgagcggc tcttcaacct tctgaagacc
     3961 ggccaccgga tggagaggcc agacaactgc agcgaggaga tgtactgcct gatgctgcaa
     4021 tgctggaagc aggagccgga caaaaggccg gtgtttgcgg acatcagcaa agacctggag
     4081 aagatgatgg ttaagaggag agactacttg gaccttgcgg cgtccactcc atctgactcc
     4141 ctgatttatg acgacggcct ctcagaggag gagacaccgc tggtggactg taataatgcc
     4201 cccctccctc gagccctccc ttccacatgg attgaaaaca aactctatgg tagaatttcc
     4261 catgcattta ctagattccg ttcagctttc ttgtacaaag tggttgatat caaggccgcc
     4321 gacatcacca gcctgtacaa gaaagtgggg tggagccatc cccagttcga gaagggcgga
     4381 ggatctggcg gcggaagcgg cggaggaagc tggtcccacc ctcagtttga gaaaggcacc
     4441 gagctgggca gcaccatggc cagctacccc tacgacgtgc ccgactacgc caaggacaac
     4501 accgtgcccc tgaagctgat cgccctgctg gccaacggcg agttccactc tggcgagcag
     4561 ctgggcgaga cactgggcat gagcagagcc gccatcaaca agcacatcca gaccctgcgg
     4621 gactggggcg tggacgtgtt cacagtgccc ggcaagggct acagcctgcc cgagcccatc
     4681 cagctgctga acgccaagca gatcctgggc cagctggatg gcggatctgt ggccgtgctg
     4741 cccgtgatcg acagcaccaa ccagtatctg ctcgaccgga tcggcgagct gaagtccggc
     4801 gacgcctgta tcgccgagta ccagcaggcc ggcagaggcg gcaggggcag aaagtggttc
     4861 agccccttcg gcgccaacct gtacctgagc atgttctgga gactggaaca gggcccagcc
     4921 gccgctattg gcctgagcct ggtcatcggc atcgtgatgg ccgaggtgct gcggaagctg
     4981 ggcgccgaca aagtgcgcgt gaagtggccc aacgacctgt atctgcagga ccggaagctg
     5041 gccggcatcc tggtggaact gaccggcaaa acaggcgacg ccgcccagat cgtgatcggc
     5101 gctggcatca acatggccat gcggcgggtg gaagagagcg tggtcaacca gggctggatc
     5161 accctgcagg aagccggcat caacctggac cggaacaccc tggccgccat gctgatcaga
     5221 gagctgagag ccgccctgga actgttcgag caggaaggcc tggcccccta cctgagcaga
     5281 tgggagaagc tggacaactt catcaaccgg cccgtgaaac tgatcatcgg cgacaaagag
     5341 atcttcggca tcagccgggg catcgacaag cagggggccc tgctcctgga acaggacggc
     5401 atcatcaagc cctggatggg cggcgagatc agcctgcgga gcgccgagaa gtgatgactc
     5461 gagtctagag ggcccgttta aacccgctga tcagcctcga ctgtgccttc tagttgccag
     5521 ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact
     5581 gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt
     5641 ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat
     5701 gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg
     5761 gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     5821 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
     5881 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     5941 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
     6001 cgtacctaga agttcctatt ccgaagttcc tattctctag aaagtatagg aacttccttg
     6061 gccaaaaagc ctgaactcac cgcgacgtct gtcgagaagt ttctgatcga aaagttcgac
     6121 agcgtctccg acctgatgca gctctcggag ggcgaagaat ctcgtgcttt cagcttcgat
     6181 gtaggagggc gtggatatgt cctgcgggta aatagctgcg ccgatggttt ctacaaagat
     6241 cgttatgttt atcggcactt tgcatcggcc gcgctcccga ttccggaagt gcttgacatt
     6301 ggggaattca gcgagagcct gacctattgc atctcccgcc gtgcacaggg tgtcacgttg
     6361 caagacctgc ctgaaaccga actgcccgct gttctgcagc cggtcgcgga ggccatggat
     6421 gcgatcgctg cggccgatct tagccagacg agcgggttcg gcccattcgg accgcaagga
     6481 atcggtcaat acactacatg gcgtgatttc atatgcgcga ttgctgatcc ccatgtgtat
     6541 cactggcaaa ctgtgatgga cgacaccgtc agtgcgtccg tcgcgcaggc tctcgatgag
     6601 ctgatgcttt gggccgagga ctgccccgaa gtccggcacc tcgtgcacgc ggatttcggc
     6661 tccaacaatg tcctgacgga caatggccgc ataacagcgg tcattgactg gagcgaggcg
     6721 atgttcgggg attcccaata cgaggtcgcc aacatcttct tctggaggcc gtggttggct
     6781 tgtatggagc agcagacgcg ctacttcgag cggaggcatc cggagcttgc aggatcgccg
     6841 cggctccggg cgtatatgct ccgcattggt cttgaccaac tctatcagag cttggttgac
     6901 ggcaatttcg atgatgcagc ttgggcgcag ggtcgatgcg acgcaatcgt ccgatccgga
     6961 gccgggactg tcgggcgtac acaaatcgcc cgcagaagcg cggccgtctg gaccgatggc
     7021 tgtgtagaag tactcgccga tagtggaaac cgacgcccca gcactcgtcc gagggcaaag
     7081 gaatagcacg tactacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc
     7141 ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag
     7201 ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     7261 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     7321 ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctagctagag cttggcgtaa
     7381 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
     7441 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
     7501 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
     7561 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     7621 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     7681 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     7741 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     7801 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     7861 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     7921 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     7981 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     8041 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     8101 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     8161 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     8221 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     8281 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     8341 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     8401 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     8461 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     8521 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     8581 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     8641 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     8701 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     8761 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     8821 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     8881 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     8941 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     9001 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     9061 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     9121 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     9181 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     9241 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     9301 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     9361 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     9421 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     9481 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     9541 gtc
//
