LOCUS       sequence-270464-        8580 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  StrepIII-HA-tagged gene expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8580)
  AUTHORS   Yadav L, Pietila E, Ohman T, Liu X, Mahato AK, Sidorova Y, Lehti K, 
            Saarma M, Varjosalo M
  TITLE     PTPRA Phosphatase Regulates GDNF-Dependent RET Signaling and 
            Inhibits the RET Mutant MEN2A Oncogenic Potential.
  JOURNAL   iScience. 2020 Feb 21;23(2):100871. doi: 10.1016/j.isci.2020.100871.
            Epub 2020 Jan 31.
  PUBMED    32062451
REFERENCE   2  (bases 1 to 8580)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "iScience.";
            date: "2020-02-21"; volume: "23(2):100871. doi"; pages: " 
            10.1016/j.isci.2020.100871. Epub 2020 Jan 31"
COMMENT     Sequence Label: MEN2A-SHC
FEATURES             Location/Qualifiers
     source          1..8580
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    820..838
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    841..859
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     869..893
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    1036..1060
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4277..4301)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4348..4371
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             4408..4431
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             4462..4488
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             4462..4488
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(4532..4549)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4538..4762
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(4895..4914)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    5046..5093
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             5101..6123
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             5101..6123
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    6253..6374
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6290..6309)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6344..6363
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6423..6439)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6423..6439)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6436..6458)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6447..6463
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6471..6477,6478..6495,6496..6501))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6516..6537
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6654..6671)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6825..7413)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6905..6924)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7584..8375,8376..8444))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8207..8226
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8445..8549)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ccctatcagt gatagagatc
      841 tccctatcag tgatagagat cgtcgacgag ctcgtttagt gaaccgtcag atcgcctgga
      901 gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgga
      961 ctctagcgtt taaacttaag cttggtaccg agctcggatc cactagtcca gtgtggtgga
     1021 attctgcaga tatcaacaag tttgtacaaa aaagttggca ccatggcgaa ggcgacgtcc
     1081 ggtgccgcgg ggctgcgtct gctgttgctg ctgctgctgc cgctgctagg caaagtggca
     1141 ttgggcctct acttctcgag ggatgcttac tgggagaagc tgtatgtgga ccaggcggcc
     1201 ggcacgccct tgctgtacgt ccatgccctg cgggacgccc ctgaggaggt gcccagcttc
     1261 cgcctgggcc agcatctcta cggcacgtac cgcacacggc tgcatgagaa caactggatc
     1321 tgcatccagg aggacaccgg cctcctctac cttaaccgga gcctggacca tagctcctgg
     1381 gagaagctca gtgtccgcaa ccgcggcttt cccctgctca ccgtctacct caaggtcttc
     1441 ctgtcaccca catcccttcg tgagggcgag tgccagtggc caggctgtgc ccgcgtatac
     1501 ttctccttct tcaacacctc ctttccagcc tgcagctccc tcaagccccg ggagctctgc
     1561 ttcccagaga caaggccctc cttccgcatt cgggagaacc gacccccagg caccttccac
     1621 cagttccgcc tgctgcctgt gcagttcttg tgccccaaca tcagcgtggc ctacaggctc
     1681 ctggagggtg agggtctgcc cttccgctgc gccccggaca gcctggaggt gagcacgcgc
     1741 tgggccctgg accgcgagca gcgggagaag tacgagctgg tggccgtgtg caccgtgcac
     1801 gccggcgcgc gcgaggaggt ggtgatggtg cccttcccgg tgaccgtgta cgacgaggac
     1861 gactcggcgc ccaccttccc cgcgggcgtc gacaccgcca gcgccgtggt ggagttcaag
     1921 cggaaggagg acaccgtggt ggccacgctg cgtgtcttcg atgcagacgt ggtacctgca
     1981 tcaggggagc tggtgaggcg gtacacaagc acgctgctcc ccggggacac ctgggcccag
     2041 cagaccttcc gggtggaaca ctggcccaac gagacctcgg tccaggccaa cggcagcttc
     2101 gtgcgggcga ccgtacatga ctataggctg gttctcaacc ggaacctctc catctcggag
     2161 aaccgcacca tgcagctggc ggtgctggtc aatgactcag acttccaggg cccaggagcg
     2221 ggcgtcctct tgctccactt caacgtgtcg gtgctgccgg tcagcctgca cctgcccagt
     2281 acctactccc tctccgtgag caggagggct cgccgatttg cccagatcgg gaaagtctgt
     2341 gtggaaaact gccaggcatt cagtggcatc aacgtccagt acaagctgca ttcctctggt
     2401 gccaactgca gcacgctagg ggtggtcacc tcagccgagg acacctcggg gatcctgttt
     2461 gtgaatgaca ccaaggccct gcggcggccc aagtgtgccg aacttcacta catggtggtg
     2521 gccaccgacc agcagacctc taggcaggcc caggcccagc tgcttgtaac agtggagggg
     2581 tcatatgtgg ccgaggaggc gggctgcccc ctgtcctgtg cagtcagcaa gagacggctg
     2641 gagtgtgagg agtgtggcgg cctgggctcc ccaacaggca ggtgtgagtg gaggcaagga
     2701 gatggcaaag ggatcaccag gaacttctcc acctgctctc ccagcaccaa gacctgcccc
     2761 gacggccact gcgatgttgt ggagacccaa gacatcaaca tttgccctca ggactgcctc
     2821 cggggcagca ttgttggggg acacgagcct ggggagcccc gggggattaa agctggctat
     2881 ggcacctgca actgcttccc tgaggaggag aagtgcttct gcgagcccga agacatccag
     2941 gatccactgt gcgacgagct gtggcgcacg gtgatcgcag ccgctgtcct cttctccttc
     3001 atcgtctcgg tgctgctgtc tgccttctgc atccactgct accacaagtt tgcccacaag
     3061 ccacccatct cctcagctga gatgaccttc cggaggcccg cccaggcctt cccggtcagc
     3121 tactcctctt ccagtgcccg ccggccctcg ctggactcca tggagaacca ggtctccgtg
     3181 gatgccttca agatcctgga ggatccaaag tgggaattcc ctcggaagaa cttggttctt
     3241 ggaaaaactc taggagaagg cgaatttgga aaagtggtca aggcaacggc cttccatctg
     3301 aaaggcagag cagggtacac cacggtggcc gtgaagatgc tgaaagagaa cgcctccccg
     3361 agtgagcttc gagacctgct gtcagagttc aacgtcctga agcaggtcaa ccacccacat
     3421 gtcatcaaat tgtatggggc ctgcagccag gatggcccgc tcctcctcat cgtggagtac
     3481 gccaaatacg gctccctgcg gggcttcctc cgcgagagcc gcaaagtggg gcctggctac
     3541 ctgggcagtg gaggcagccg caactccagc tccctggacc acccggatga gcgggccctc
     3601 accatgggcg acctcatctc atttgcctgg cagatctcac aggggatgca gtatctggcc
     3661 gagatgaagc tcgttcatcg ggacttggca gccagaaaca tcctggtagc tgaggggcgg
     3721 aagatgaaga tttcggattt cggcttgtcc cgagatgttt atgaagagga ttcgtacgtg
     3781 aagaggagcc agggtcggat tccagttaaa tggatggcaa ttgaatccct ttttgatcat
     3841 atctacacca cgcaaagtga tgtatggtct tttggtgtcc tgctgtggga gatcgtgacc
     3901 ctagggggaa acccctatcc tgggattcct cctgagcggc tcttcaacct tctgaagacc
     3961 ggccaccgga tggagaggcc agacaactgc agcgaggaga tgtactgcct gatgctgcaa
     4021 tgctggaagc aggagccgga caaaaggccg gtgtttgcgg acatcagcaa agacctggag
     4081 aagatgatgg ttaagaggag agactacttg gaccttgcgg cgtccactcc atctgactcc
     4141 ctgatttatg acgacggcct ctcagaggag gagacaccgc tggtggactg taataatgcc
     4201 cccctccctc gagccctccc ttccacatgg attgaaaaca aactctatgg tagaatttcc
     4261 catgcattta ctagattccc aactttcttg tacaaagtgg ttgatatcaa ggccgccgac
     4321 atcaccagcc tgtacaagaa agtggggtgg agccatcccc agttcgagaa gggcggagga
     4381 tctggcggcg gaagcggcgg aggaagctgg tcccaccctc agtttgagaa aggcaccgag
     4441 ctgggcagca ccatggccag ctacccctac gacgtgcccg actacgcctg atgactcgag
     4501 tctagagggc ccgtttaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca
     4561 tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc
     4621 ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg
     4681 gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct
     4741 ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg
     4801 tatccccacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     4861 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     4921 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     4981 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
     5041 acctagaagt tcctattccg aagttcctat tctctagaaa gtataggaac ttccttggcc
     5101 aaaaagcctg aactcaccgc gacgtctgtc gagaagtttc tgatcgaaaa gttcgacagc
     5161 gtctccgacc tgatgcagct ctcggagggc gaagaatctc gtgctttcag cttcgatgta
     5221 ggagggcgtg gatatgtcct gcgggtaaat agctgcgccg atggtttcta caaagatcgt
     5281 tatgtttatc ggcactttgc atcggccgcg ctcccgattc cggaagtgct tgacattggg
     5341 gaattcagcg agagcctgac ctattgcatc tcccgccgtg cacagggtgt cacgttgcaa
     5401 gacctgcctg aaaccgaact gcccgctgtt ctgcagccgg tcgcggaggc catggatgcg
     5461 atcgctgcgg ccgatcttag ccagacgagc gggttcggcc cattcggacc gcaaggaatc
     5521 ggtcaataca ctacatggcg tgatttcata tgcgcgattg ctgatcccca tgtgtatcac
     5581 tggcaaactg tgatggacga caccgtcagt gcgtccgtcg cgcaggctct cgatgagctg
     5641 atgctttggg ccgaggactg ccccgaagtc cggcacctcg tgcacgcgga tttcggctcc
     5701 aacaatgtcc tgacggacaa tggccgcata acagcggtca ttgactggag cgaggcgatg
     5761 ttcggggatt cccaatacga ggtcgccaac atcttcttct ggaggccgtg gttggcttgt
     5821 atggagcagc agacgcgcta cttcgagcgg aggcatccgg agcttgcagg atcgccgcgg
     5881 ctccgggcgt atatgctccg cattggtctt gaccaactct atcagagctt ggttgacggc
     5941 aatttcgatg atgcagcttg ggcgcagggt cgatgcgacg caatcgtccg atccggagcc
     6001 gggactgtcg ggcgtacaca aatcgcccgc agaagcgcgg ccgtctggac cgatggctgt
     6061 gtagaagtac tcgccgatag tggaaaccga cgccccagca ctcgtccgag ggcaaaggaa
     6121 tagcacgtac tacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga
     6181 atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc
     6241 ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     6301 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     6361 atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt ggcgtaatca
     6421 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
     6481 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
     6541 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
     6601 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     6661 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     6721 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     6781 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     6841 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     6901 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     6961 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     7021 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     7081 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     7141 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     7201 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     7261 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     7321 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     7381 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     7441 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     7501 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     7561 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     7621 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     7681 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     7741 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     7801 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     7861 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     7921 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     7981 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     8041 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     8101 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     8161 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     8221 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     8281 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     8341 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     8401 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     8461 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     8521 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
//
