LOCUS       sequence-270491-        1355 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Protein expression/GST pull-down.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1355)
  TITLE     Opher Gileadi SGC expression vectors
REFERENCE   2  (bases 1 to 1355)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: TBXTA-c024
FEATURES             Location/Qualifiers
     source          1..1355
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..24
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             31..684
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGADHPPK"
     primer_bind     642..664
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     CDS             712..732
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 catatgcacc atcatcatca tcattcttct atgtccccta tactaggtta ttggaaaatt
       61 aagggccttg tgcaacccac tcgacttctt ttggaatatc ttgaagaaaa atatgaagag
      121 catttgtatg agcgcgatga aggtgataaa tggcgaaaca aaaagtttga attgggtttg
      181 gagtttccca atcttcctta ttatattgat ggtgatgtta aattaacaca gtctatggcc
      241 atcatacgtt atatagctga caagcacaac atgttgggtg gttgtccaaa agagcgtgca
      301 gagatttcaa tgcttgaagg agcggttttg gatattagat acggtgtttc gagaattgca
      361 tatagtaaag actttgaaac tctcaaagtt gattttctta gcaagctacc tgaaatgctg
      421 aaaatgttcg aagatcgttt atgtcataaa acatatttaa atggtgatca tgtaacccat
      481 cctgacttca tgttgtatga cgctcttgat gttgttttat acatggaccc aatgtgcctg
      541 gatgcgttcc caaaattagt ttgttttaaa aaacgtattg aagctatccc acaaattgat
      601 aagtacttga aatccagcaa gtatatagca tggcctttgc agggctggca agccacgttt
      661 ggtggtgccg accatcctcc aaaatcgagc tcaggtgtag atctgggtac cgagaacctg
      721 tacttccaat ccatggaact gcgtgtgggt ctggaagaaa gcgaactgtg gctgcgcttt
      781 aaagagctga ccaacgaaat gatcgtgacc aaaaacggcc gccgcatgtt tcctgtgctg
      841 aaagttaacg tgagcggcct ggacccgaac gccatgtaca gcttcctgct ggatttcgtt
      901 gccgcagata accaccgctg gaagtatgtg aacggcgaat gggtgccggg tggcaaaccg
      961 gaaccgcagg cacctagctg cgtgtatatc cacccggata gccctaactt cggtgcccat
     1021 tggatgaaag ccccggtgag cttcagcaag gtgaagctga ccaacaaact gaacggcggc
     1081 ggccaaatca tgctgaacag cttacataaa tatgaacctc gcatccacat tgtgcgtgtg
     1141 ggcgacccgc aacgtatgat caccagccat tgcttcccgg agacacagtt cattgccgtt
     1201 accgcctacc agaacgagga aattaccgca ctgaaaatta aatacaaccc ttttgcaaaa
     1261 gccttcttag atgccaaaga gcgcagcgac tgacagtaaa ggtggatacg gatccgaatt
     1321 cgagctccgt cgacaagctt gcggccgcac tcgag
//
