LOCUS       sequence-270493-        2108 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Protein expression/GST pull-down.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2108)
  TITLE     Opher Gileadi SGC expression vectors
REFERENCE   2  (bases 1 to 2108)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: TBXTA-c025
FEATURES             Location/Qualifiers
     source          1..2108
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..24
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             31..684
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGADHPPK"
     primer_bind     642..664
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     CDS             712..732
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 catatgcacc atcatcatca tcattcttct atgtccccta tactaggtta ttggaaaatt
       61 aagggccttg tgcaacccac tcgacttctt ttggaatatc ttgaagaaaa atatgaagag
      121 catttgtatg agcgcgatga aggtgataaa tggcgaaaca aaaagtttga attgggtttg
      181 gagtttccca atcttcctta ttatattgat ggtgatgtta aattaacaca gtctatggcc
      241 atcatacgtt atatagctga caagcacaac atgttgggtg gttgtccaaa agagcgtgca
      301 gagatttcaa tgcttgaagg agcggttttg gatattagat acggtgtttc gagaattgca
      361 tatagtaaag actttgaaac tctcaaagtt gattttctta gcaagctacc tgaaatgctg
      421 aaaatgttcg aagatcgttt atgtcataaa acatatttaa atggtgatca tgtaacccat
      481 cctgacttca tgttgtatga cgctcttgat gttgttttat acatggaccc aatgtgcctg
      541 gatgcgttcc caaaattagt ttgttttaaa aaacgtattg aagctatccc acaaattgat
      601 aagtacttga aatccagcaa gtatatagca tggcctttgc agggctggca agccacgttt
      661 ggtggtgccg accatcctcc aaaatcgagc tcaggtgtag atctgggtac cgagaacctg
      721 tacttccaat ccatgtccaa tagcagccct ggcaccgaaa gcgccggtaa gagcctacag
      781 taccgtgtgg accatttact gagcgccgtg gagaacgaac tacaggcagg cagcgaaaaa
      841 ggtgacccga ccgaacgcga actgcgtgtg ggtctggaag aaagcgaact gtggctgcgc
      901 tttaaagagc tgaccaacga aatgatcgtg accaaaaacg gccgccgcat gtttcctgtg
      961 ctgaaagtta acgtgagcgg cctggacccg aacgccatgt acagcttcct gctggatttc
     1021 gttgccgcag ataaccaccg ctggaagtat gtgaacggcg aatgggtgcc gggtggcaaa
     1081 ccggaaccgc aggcacctag ctgcgtgtat atccacccgg atagccctaa cttcggtgcc
     1141 cattggatga aagccccggt gagcttcagc aaggtgaagc tgaccaacaa actgaacggc
     1201 ggcggccaaa tcatgctgaa cagcttacat aaatatgaac ctcgcatcca cattgtgcgt
     1261 gtgggcgacc cgcaacgtat gatcaccagc cattgcttcc cggagacaca gttcattgcc
     1321 gttaccgcct accagaacga ggaaattacc gcactgaaaa ttaaatacaa cccttttgca
     1381 aaagccttct tagatgccaa agagcgcagc gaccacaaag aaatgatgga ggaaccgggc
     1441 gacagccagc agcctggtta tagccaatgg ggctggctgc tgccgggtac cagtacactg
     1501 tgcccgccgg caaacccgca tcctcagttt ggtggcgccc tgagcttacc gagcacccac
     1561 agttgtgatc gctatcctac cctgcgtagc catcgtagca gtccgtaccc gagcccgtat
     1621 gcacatcgta acaacagccc gacatatagc gacaacagcc cggcctgtct gagcatgcta
     1681 cagagccatg ataactggag cagcctgggc atgccggcac atccgagcat gctgccggtg
     1741 agtcacaatg ccagcccgcc gaccagtagc agccagtatc ctagcctgtg gagcgttagt
     1801 aacggtgcag tgaccccggg tagccaggca gcagcagtta gcaatggtct gggcgcccag
     1861 ttttttcgcg gcagtccggc acattacacc ccgctgaccc atcctgtgag tgccccgagc
     1921 agtagtggta gcccgctgta tgaaggtgcc gcagccgcca ccgacattgt tgacagccag
     1981 tatgatgccg ccgcacaggg tcgcctgatt gcaagctgga cccctgtgag tccgccgagc
     2041 atgtgacagt aaaggtggat acggatccga attcgagctc cgtcgacaag cttgcggccg
     2101 cactcgag
//
