LOCUS       sequence-270501-         690 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Protein expression/Streptavidin pull-down.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 690)
  TITLE     Opher Gileadi SGC expression vectors
REFERENCE   2  (bases 1 to 690)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: TBXTA-c043
FEATURES             Location/Qualifiers
     source          1..690
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..21
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             46..66
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             643..687
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
ORIGIN
        1 atgcaccatc atcatcatca ttcttctggt gtagatctgg gtaccgagaa cctgtacttc
       61 caatccatgg aactgcgtgt gggtctggaa gaaagcgaac tgtggctgcg ctttaaagag
      121 ctgaccaacg aaatgatcgt gaccaaaaac ggccgccgca tgtttcctgt gctgaaagtt
      181 aacgtgagcg gcctggaccc gaacgccatg tacagcttcc tgctggattt cgttgccgca
      241 gataaccacc gctggaagta tgtgaacggc gaatgggtgc cgggtggcaa accggaaccg
      301 caggcaccta gctgcgtgta tatccacccg gatagcccta acttcggtgc ccattggatg
      361 aaagccccgg tgagcttcag caaggtgaag ctgaccaaca aactgaacgg cggcggccaa
      421 atcatgctga acagcttaca taaatatgaa cctcgcatcc acattgtgcg tgtgggcggc
      481 ccgcaacgta tgatcaccag ccattgcttc ccggagacac agttcattgc cgttaccgcc
      541 taccagaacg aggaaattac cgcactgaaa attaaataca acccttttgc aaaagccttc
      601 ttagatgcca aagagcgcag cgacagcagt aaaggtggat acggcctgaa tgatatcttt
      661 gaagcgcaga agattgaatg gcatgaatga
//
