LOCUS       sequence-270529-        8503 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  lentiviral destination vector with CAMKIIA promoter, C-terminal V5 
            tag, SV40 promoter, and Zeo Resistance gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8503)
  TITLE     Barrett lab unpublished plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8503)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pSMPUW_8.3_M4_CAMKIIA-gateway-V5-SV40-Zeo
FEATURES             Location/Qualifiers
     source          1..8503
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             522..702
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    749..873
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    908..1024
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1078..2367
                     /label=CaMKII promoter
                     /note="promoter for the mouse calcium/calmodulin-dependent 
                     kinase II alpha subunit"
     protein_bind    2379..2503
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(2528..2533,2534..2551,2552..2558)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             2612..3271
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(2678..2697)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             3613..3918
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     3797..3816
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(3959..4083)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             4085..4126
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     promoter        4221..4431
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        4243..4439
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      4290..4425
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     regulatory      4476..4485
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4482..4856
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     misc_feature    4929..5517
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4982..5002)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5400..5411)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5707..5887
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        5956..6166
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      6025..6160
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    6276..6397
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     6367..6386
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6449..6466)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6620..7208)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6700..6719)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(7460..7499)
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     CDS             complement(7509..8324)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(7509..8324)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     8232..8251
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     8316..8335
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgggtctctc tggttagacc
      541 agatttgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
      601 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
      661 gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga
      721 cctgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc
      781 gcgcacggca agaggcgagg ggcggcgact gcagagtacg ccaaaatttt gactagcgga
      841 ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggaa aatagcggcc
      901 gccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag
      961 acataatagc aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa
     1021 ttttcggggg atccgcggaa ttcgtccacg gtccaatctc ggtccgaacg agatatccat
     1081 tatggcctta ggtcacttca tctccatggg gttcttcttc tgattttcta gaaaatgaga
     1141 tgggggtgca gagagcttcc tcagtgacct gcccagggtc acatcagaaa tgtcagagct
     1201 agaacttgaa ctcagattac taatcttaaa ttccatgcct tgggggcatg caagtacgat
     1261 atacagaagg agtgaactca ttagggcaga tgaccaatga gtttaggaaa gaagagtcca
     1321 gggcagggta catctacacc acccgcccag ccctgggtga gtccagccac gttcacctca
     1381 ttatagttgc ctctctccag tcctaccttg acgggaagca caagcagaaa ctgggacagg
     1441 agccccagga gaccaaatct tcatggtccc tctgggagga tgggtgggga gagctgtggc
     1501 agaggcctca ggaggggccc tgctgctcag tggtgacaga taggggtgag aaagcagaca
     1561 gagtcattcc gtcagcattc tgggtctgtt tggtacttct tctcacgcta aggtggcggt
     1621 gtgatatgca caatggctaa aaagcaggga gagctggaaa gaaacaagga cagagacaga
     1681 ggccaagtca accagaccaa ttcccagagg aagcaaagaa accattacag agactacaag
     1741 ggggaaggga aggagagatg aattagcttc ccctgtaaac cttagaaccc agctgttgcc
     1801 agggcaacgg ggcaatacct gtctcttcag aggagatgaa gttgccaggg taactacatc
     1861 ctgtctttct caaggaccat cccagaatgt ggcacccact agccgttacc atagcaactg
     1921 cctctttgcc ccacttaatc ccatcccgtc tgttaaaagg gccctatagt tggaggtggg
     1981 ggaggtagga agagcgatga tcacttgtgg actaagtttg ttcacatccc cttctccaac
     2041 cccctcagta catcaccctg ggagaacaag gtccacttgc ttctgggccc acacagtcct
     2101 gcagtattgt gtatataagg ccagggcaac ggaggagcag gttttgaagt gaaaggcagg
     2161 caggtgttgg ggaggcagtt accggggcaa cgggaacagg gcgtttcgga ggtggttgcc
     2221 atggggacct ggatgctgac gaaggctcgc gaggctgtga gcagccacag tgccctgctc
     2281 agaagcccca agctcgtcaa tcaagctggt tctccatttg cactcaggag cacgggcagg
     2341 cgagtggccc ctagttctgg gggcagcggg gcgcgccaac aagtttgtac aaaaaagctg
     2401 aacgagaaac gtaaaatgat ataaatatca atatattaaa ttagattttg cataaaaaac
     2461 agactacata atactgtaaa acacaacata tccagtcact atggcggccg cattaggcac
     2521 cccaggcttt acactttatg cttccggctc gtataatgtg tggattttga gttaggatcc
     2581 gtcgagattt tcaggagcta aggaagctaa aatggagaaa aaaatcactg gatataccac
     2641 cgttgatata tcccaatggc atcgtaaaga acattttgag gcatttcagt cagttgctca
     2701 atgtacctat aaccagaccg ttcagctgga tattacggcc tttttaaaga ccgtaaagaa
     2761 aaataagcac aagttttatc cggcctttat tcacattctt gcccgcctga tgaatgctca
     2821 tccggaattc cgtatggcaa tgaaagacgg tgagctggtg atatgggata gtgttcaccc
     2881 ttgttacacc gttttccatg agcaaactga aacgttttca tcgctctgga gtgaatacca
     2941 cgacgatttc cggcagtttc tacacatata ttcgcaagat gtggcgtgtt acggtgaaaa
     3001 cctggcctat ttccctaaag ggtttattga gaatatgttt ttcgtctcag ccaatccctg
     3061 ggtgagtttc accagttttg atttaaacgt ggccaatatg gacaacttct tcgcccccgt
     3121 tttcaccatg ggcaaatatt atacgcaagg cgacaaggtg ctgatgccgc tggcgattca
     3181 ggttcatcat gccgtttgtg atggcttcca tgtcggcaga atgcttaatg aattacaaca
     3241 gtactgcgat gagtggcagg gcggggcgta aagatctgga tccggcttac taaaagccag
     3301 ataacagtat gcgtatttgc gcgctgattt ttgcggtata agaatatata ctgatatgta
     3361 tacccgaagt atgtcaaaaa gaggtatgct atgaagcagc gtattacagt gacagttgac
     3421 agcgacagct atcagttgct caaggcatat atgatgtcaa tatctccggt ctggtaagca
     3481 caaccatgca gaatgaagcc cgtcgtctgc gtgccgaacg ctggaaagcg gaaaatcagg
     3541 aagggatggc tgaggtcgcc cggtttattg aaatgaacgg ctcttttgct gacgagaaca
     3601 ggggctggtg aaatgcagtt taaggtttac acctataaaa gagagagccg ttatcgtctg
     3661 tttgtggatg tacagagtga tattattgac acgcccgggc gacggatggt gatccccctg
     3721 gccagtgcac gtctgctgtc agataaagtc tcccgtgaac tttacccggt ggtgcatatc
     3781 ggggatgaaa gctggcgcat gatgaccacc gatatggcca gtgtgccggt ctccgttatc
     3841 ggggaagaag tggctgatct cagccaccgc gaaaatgaca tcaaaaacgc cattaacctg
     3901 atgttctggg gaatataaat gtcaggctcc cttatacaca gccagtctgc aggtcgacca
     3961 tagtgactgg atatgttgtg ttttacagta ttatgtagtc tgttttttat gcaaaatcta
     4021 atttaatata ttgatattta tatcatttta cgtttctcgt tcagctttct tgtacaaagt
     4081 ggttggtaag cctatcccta accctctcct cggtctcgat tctacgtagt aatgaacctt
     4141 aggagccaac agctaagcac acacttcgaa tgcatctcaa ttagtcagca accaggtgtg
     4201 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcgtctc aattagtcag
     4261 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
     4321 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
     4381 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa
     4441 agctttctag aggtaccacc ggagcttatt taaatgccac catggccaag ttgaccagtg
     4501 ccgttccggt gctcaccgcg cgcgacgtcg ccggagcggt cgagttctgg accgaccggc
     4561 tcgggttctc ccgggacttc gtggaggacg acttcgccgg tgtggtccgg gacgacgtga
     4621 ccctgttcat cagcgcggtc caggaccagg tggtgccgga caacaccctg gcctgggtgt
     4681 gggtgcgcgg cctggacgag ctgtacgccg agtggtcgga ggtcgtgtcc acgaacttcc
     4741 gggacgcctc cgggccggcc atgaccgaga tcggcgagca gccgtggggg cgggagttcg
     4801 ccctgcgcga cccggccggc aactgcgtgc acttcgtggc cgaggagcag gactaattaa
     4861 ttaacacaaa tacgcgtatc tgcagtcgac tttgccagac cggagtccga acacagctct
     4921 tccagcataa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact
     4981 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg
     5041 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg
     5101 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa
     5161 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc
     5221 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg
     5281 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt
     5341 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt
     5401 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc
     5461 cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgctta
     5521 gtactggtac ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta
     5581 aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga ttccggaatt
     5641 tatttgtgaa atttgtgatg ctattgcttt atttgtaaac cggtgcagct gctttttgcc
     5701 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
     5761 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
     5821 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
     5881 tctagcatct agagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag
     5941 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     6001 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
     6061 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct
     6121 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggctagag atcataatca
     6181 gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga
     6241 acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg
     6301 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     6361 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgctag ctgagcgggc
     6421 ttttttttct taggccttct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     6481 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     6541 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     6601 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     6661 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     6721 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     6781 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     6841 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     6901 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     6961 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     7021 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     7081 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     7141 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     7201 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     7261 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     7321 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     7381 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     7441 ttgcctgact cctgcgcagt ccaaaaaaaa aggctccaaa aggagccttt aattgtatcg
     7501 gtgggccctt agaaaaactc atcgagcatc aaatgaaact gcaatttatt catatcagga
     7561 ttatcaatac catatttttg aaaaagccgt ttctgtaatg aaggagaaaa ctcaccgagg
     7621 cagttccata ggatggcaag atcctggtat cggtctgcga ttccgactcg tccaacatca
     7681 atacaaccta ttaatttccc ctcgtcaaaa ataaggttat caagtgagaa atcaccatga
     7741 gtgacgactg aatccggtga gaatggcaaa agcttatgca tttctttcca gacttgttca
     7801 acaggccagc cattacgctc gtcatcaaaa tcactcgcat caaccaaacc gttattcatt
     7861 cgtgattgcg cctgagcgag acgaaatacg cgatcgctgt taaaaggaca attacaaaca
     7921 ggaatcgaat gcaaccggcg caggaacact gccagcgcat caacaatatt ttcacctgaa
     7981 tcaggatatt cttctaatac ctggaatgct gttttcccgg ggatcgcagt ggtgagtaac
     8041 catgcatcat caggagtacg gataaaatgc ttgatggtcg gaagaggcat aaattccgtc
     8101 agccagttta gtctgaccat ctcatctgta acatcattgg caacgctacc tttgccatgt
     8161 ttcagaaaca actctggcgc atcgggcttc ccatacaatc gatagattgt cgcacctgat
     8221 tgcccgacat tatcgcgagc ccatttatac ccatataaat cagcatccat gttggaattt
     8281 aatcgcggcc tcgagcaaga cgtttcccgt tgaatatggc tcataacacc ccttgtatta
     8341 ctgtttatgt aagcagacag ttttattgtt catgatgata tatttttatc ttgtgcaatg
     8401 taacatcaga gattttgaga cacaacgtgg tttaaacaaa tagtcaaaag cctccgggcg
     8461 actagtcggg gtcattagtt catagcccat atatggagtt ccg
//
