LOCUS       sequence-270550-        4469 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CmR, Para-popZ-rbs-msfGFPC-H3H4. Replicative plasmid for the 
            expression of bait. C-terminal GFP fusion..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4469)
  AUTHORS   Lim HC, Bernhardt TG
  TITLE     A PopZ-Linked Apical Recruitment Assay for Studying Protein-Protein 
            Interactions in the Bacterial Cell Envelope.
  JOURNAL   Mol Microbiol. 2019 Sep 24. doi: 10.1111/mmi.14391.
  PUBMED    31550057
REFERENCE   2  (bases 1 to 4469)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol 
            Microbiol. 2019 Sep 24. doi: 10.1111/mmi.14391."
COMMENT     Sequence Label: pHCL150
FEATURES             Location/Qualifiers
     source          1..4469
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..285
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     208..227
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     CDS             2207..2398
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     complement(2392..2414)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    2500..2640
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     2555..2574
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(2655..2672)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2826..3414)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2906..2925)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(3585..4244)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPELRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     4159..4178
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(4245..4347)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
ORIGIN
        1 aagaaaccaa ttgtccatat tgcatcagac attgccgtca ctgcgtcttt tactggctct
       61 tctcgctaac caaaccggta accccgctta ttaaaagcat tctgtaacaa agcgggacca
      121 aagccatgac aaaaacgcgt aacaaaagtg tctataatca cggcagaaaa gtccacattg
      181 attatttgca cggcgtcaca ctttgctatg ccatagcatt tttatccata agattagcga
      241 atcctacctg acgcttttta tcgcaactct ctactgtttc tccatacccg tttttttggg
      301 tctagcagga gatacatata tgtccgatca gtctcaagaa cctacaatgg aggaaatcct
      361 cgcctccatt cgacgcatca tctcggagga tgacgcgccg gcggagcctg cggccgaagc
      421 ggcgcccccg ccgccgccgg aacccgaacc tgaaccggtg tcgttcgacg acgaggttct
      481 ggaattgacg gacccgatcg cgcccgagcc cgagctgccg ccgctggaga ctgtcggcga
      541 catcgacgtc tattcgccgc cggaacctga gtcggaaccg gcctacacgc cgccgccggc
      601 ggctccggtg tttgatcgcg acgaagtcgc cgagcagctg gtcggcgttt cggccgcttc
      661 ggccgcggcg agcgccttcg gcagcctgag ctcggccctg ctgatgccca aggacggtcg
      721 gacgctggaa gacgtcgtac gcgagctgct gcgcccgctg ctcaaggagt ggctggacca
      781 gaacctgccg cgcatcgtcg agaccaaggt tgaggaagaa gtgcagcgta tctctcgggg
      841 acgcggctaa cgctgtctag atttaagaag gagatataca tatgctcgag ggtggaggct
      901 ctggtggcgg ttctaaggga gaagagttgt ttacgggtgt tgtaccgatt cttgtcgaac
      961 tggacggaga tgtgaatgga cataaatttt cggtccgtgg cgagggagag ggtgacgcta
     1021 ccaacggaaa actcactctt aagttcatct gtaccaccgg caagctgcct gtcccatggc
     1081 caacccttgt gacgactctg acttatggcg tgcaatgttt ttcgcggtat cccgaccaca
     1141 tgaaacagca tgattttttc aagtcggcga tgccagaagg atacgtgcaa gagcggacga
     1201 tctccttcaa ggatgacggc acctacaaga cccgtgccga agttaagttt gagggcgata
     1261 ctttggtcaa tcggattgag ctgaaaggaa tcgatttcaa agaagacggc aatatcctgg
     1321 gacacaagct cgaatataat tttaacagcc acaatgtata tattactgcg gataaacaga
     1381 agaatggcat caaggcaaac tttaaaattc gccataacgt agaagatgga tcggtccaac
     1441 tcgccgacca ctaccagcag aacactccaa tcggagatgg cccggttttg cttccagata
     1501 accattatct cagcactcaa tcaaagctca gcaaagaccc caacgagaag cgggatcaca
     1561 tggtactgtt ggagttcgta accgctgccg gtatcacgca cggtatggac gaattgtata
     1621 aaggaggtgg ttctggtggc ggatccggag gcggtagtgc gttattaatg cctaaggatg
     1681 gccgcacact tgaagatgtt gttagagaac ttctgcgtcc tttgcttaaa gaatggcttg
     1741 atcaaaattt gcctcgcatt gttgagacga aagtggaaga agaggttcaa cgtatttcgc
     1801 ggggtagagg ctaagcttat caccgatacg cgagcgaacg tgaagcgact gctgctgcaa
     1861 aacgtctgcg acctgagcaa caacatgaat ggtcttcggt ttccgtgttt cgtaaagtct
     1921 ggaaacgcgg aagtcagcgc cctgcaccat tatgttccgg atctgcatcg caggatgctg
     1981 ctggctaccc tgtggaacac ctacatctgt attaacgaag cgctggcatt gaccctgagt
     2041 gatttttctc tggtcccgcc gcatccatac cgccagttgt ttaccctcac aacgttccag
     2101 taaccgggca tgttcatcat cagtaacccg tatcgtgagc atcctctctc gtttcatcgg
     2161 tatcattacc cccatgaaca gaaatccccc ttacacggag gcatcagtga ccaaacagga
     2221 aaaaaccgcc cttaacatgg cccgctttat cagaagccag acattaacgc ttctggagaa
     2281 actcaacgag ctggacgcgg atgaacaggc agacatctgt gaatcgcttc acgaccacgc
     2341 tgatgagctt taccgcagct gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca
     2401 catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc
     2461 ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc gcagccatga cccagtcacg
     2521 tagcgatagc ggagtgtata ctggcttaac tatgcggcat cagagcagat tgtactgaga
     2581 gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg
     2641 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     2701 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     2761 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     2821 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     2881 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     2941 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     3001 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     3061 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     3121 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     3181 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     3241 tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca
     3301 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     3361 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     3421 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     3481 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     3541 tttaaatcaa tctaaagcgg ccgccttcat ttaaatggcg cgccttacgc cccgccctgc
     3601 cactcatcgc agtactgttg taattcatta agcattctgc cgacatggaa gccatcacaa
     3661 acggcatgat gaacctgaat cgccagcggc atcagcacct tgtcgccttg cgtataatat
     3721 ttgcccatgg tgaaaacggg ggcgaagaag ttgtccatat tggccacgtt taaatcaaaa
     3781 ctggtgaaac tcacccaggg attggctgag acgaaaaaca tattctcaat aaacccttta
     3841 gggaaatagg ccaggttttc accgtaacac gccacatctt gcgaatatat gtgtagaaac
     3901 tgccggaaat cgtcgtggta ttcactccag agcgatgaaa acgtttcagt ttgctcatgg
     3961 aaaacggtgt aacaagggtg aacactatcc catatcacca gctcaccgtc tttcattgcc
     4021 atacgtaatt ccggatgagc attcatcagg cgggcaagaa tgtgaataaa ggccggataa
     4081 aacttgtgct tatttttctt tacggtcttt aaaaaggccg taatatccag ctgaacggtc
     4141 tggttatagg tacattgagc aactgactga aatgcctcaa aatgttcttt acgatgccat
     4201 tgggatatat caacggtggt atatccagtg atttttttct ccattttagc ttccttagct
     4261 cctgaaaatc tcgacaactc aaaaaatacg cccggtagtg atcttatttc attatggtga
     4321 aagttggaac ctcttacgtg ccgatcaacg tctcattttc gccaaaagtt ggcccagggc
     4381 ttcccggtat caacagggac accaggattt atttattctg cgaagtgatc ttccgtcaca
     4441 ggtagagatc tgaattccgc cattcagag
//
