LOCUS       sequence-270556-        9768 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of FLAG-tagged TAPBPR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 9768)
  AUTHORS   McShan AC, Devlin CA, Overall SA, Park J, Toor JS, Moschidi D, 
            Flores-Solis D, Choi H, Tripathi S, Procko E, Sgourakis NG
  TITLE     Molecular determinants of chaperone interactions on MHC-I for 
            folding and antigen repertoire selection.
  JOURNAL   Proc Natl Acad Sci U S A. 2019 Dec 17;116(51):25602-25613. doi: 
            10.1073/pnas.1915562116. Epub 2019 Dec 3.
  PUBMED    31796585
REFERENCE   2  (bases 1 to 9768)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1915562116"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2019-12-17- 17"; volume: "116"; issue: "51"; pages: 
            "25602-25613"
COMMENT     Sequence Label: pCEP4-FLAG-TAPBPR
FEATURES             Location/Qualifiers
     source          1..9768
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(715..733)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        801..905
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(906..974,975..1766)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1124..1143)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1937..2525
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2426..2445
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        2956..3101
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             3144..4181
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(4179..4198)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4223..4271
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        4925..5304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5305..5508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5459..5479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5505..5529
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      5557..5566
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5641..5664
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     polyA_signal    7040..7174
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7064..7083)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7118..7137
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(7317..7339)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      7899..9688
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
ORIGIN
        1 ctgctcctgc tcctgttcca ccgtgggtcc ctttgcagcc aatgcaactt ggacgttttt
       61 ggggtctccg gacaccatct ctatgtcttg gccctgatcc tgagccgccc ggggctcctg
      121 gtcttccgcc tcctcgtcct cgtcctcttc cccgtcctcg tccatggtta tcaccccctc
      181 ttctttgagg tccactgccg ccggagcctt ctggtccaga tgtgtctccc ttctctccta
      241 ggccatttcc aggtcctgta cctggcccct cgtcagacat gattcacact aaaagagatc
      301 aatagacatc tttattagac gacgctcagt gaatacaggg agtgcagact cctgccccct
      361 ccaacagccc ccccaccctc atccccttca tggtcgctgt cagacagatc caggtctgaa
      421 aattccccat cctccgaacc atcctcgtcc tcatcaccaa ttactcgcag cccggaaaac
      481 tcccgctgaa catcctcaag atttgcgtcc tgagcctcaa gccaggcctc aaattcctcg
      541 tccccctttt tgctggacgg tagggatggg gattctcggg acccctcctc ttcctcttca
      601 aggtcaccag acagagatgc tactggggca acggaagaaa agctgggtgc ggcctgtgag
      661 gatcagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg
      721 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg
      781 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
      841 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
      901 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
      961 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     1021 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     1081 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     1141 tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     1201 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     1261 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     1321 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     1381 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     1441 cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     1501 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     1561 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     1621 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     1681 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     1741 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     1801 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     1861 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     1921 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     1981 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     2041 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt
     2101 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     2161 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     2221 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     2281 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     2341 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     2401 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     2461 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     2521 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttgaagct
     2581 gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga
     2641 tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg
     2701 ccagcaagac gtagcccagc gcgtcggccc cgagatgcgc cgcgtgcggc tgctggagat
     2761 ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa
     2821 gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccctgcttc
     2881 atccccgtgg cccgttgctc gcgtttgctg gcggtgtccc cggaagaaat atatttgcat
     2941 gtctttagtt ctatgatgac acaaaccccg cccagcgtct tgtcattggc gaattcgaac
     3001 acgcagatgc agtcggggcg gcgcggtccg aggtccactt cgcatattaa ggtgacgcgt
     3061 gtggcctcga acaccgagcg accctgcagc gacccgctta acagcgtcaa cagcgtgccg
     3121 cagatcccgg ggggcaatga gatatgaaaa agcctgaact caccgcgacg tctgtcgaga
     3181 agtttctgat cgaaaagttc gacagcgtct ccgacctgat gcagctctcg gagggcgaag
     3241 aatctcgtgc tttcagcttc gatgtaggag ggcgtggata tgtcctgcgg gtaaatagct
     3301 gcgccgatgg tttctacaaa gatcgttatg tttatcggca ctttgcatcg gccgcgctcc
     3361 cgattccgga agtgcttgac attggggaat tcagcgagag cctgacctat tgcatctccc
     3421 gccgtgcaca gggtgtcacg ttgcaagacc tgcctgaaac cgaactgccc gctgttctgc
     3481 agccggtcgc ggaggccatg gatgcgatcg ctgcggccga tcttagccag acgagcgggt
     3541 tcggcccatt cggaccgcaa ggaatcggtc aatacactac atggcgtgat ttcatatgcg
     3601 cgattgctga tccccatgtg tatcactggc aaactgtgat ggacgacacc gtcagtgcgt
     3661 ccgtcgcgca ggctctcgat gagctgatgc tttgggccga ggactgcccc gaagtccggc
     3721 acctcgtgca cgcggatttc ggctccaaca atgtcctgac ggacaatggc cgcataacag
     3781 cggtcattga ctggagcgag gcgatgttcg gggattccca atacgaggtc gccaacatct
     3841 tcttctggag gccgtggttg gcttgtatgg agcagcagac gcgctacttc gagcggaggc
     3901 atccggagct tgcaggatcg ccgcggctcc gggcgtatat gctccgcatt ggtcttgacc
     3961 aactctatca gagcttggtt gacggcaatt tcgatgatgc agcttgggcg cagggtcgat
     4021 gcgacgcaat cgtccgatcc ggagccggga ctgtcgggcg tacacaaatc gcccgcagaa
     4081 gcgcggccgt ctggaccgat ggctgtgtag aagtactcgc cgatagtgga aaccgacgcc
     4141 ccagcactcg tccggatcgg gagatggggg aggctaactg aaacacggaa ggagacaata
     4201 ccggaaggaa cccgcgctat gacggcaata aaaagacaga ataaaacgca cgggtgttgg
     4261 gtcgtttgtt cataaacgcg gggttcggtc ccagggctgg cactctgtcg ataccccacc
     4321 gagaccccat tggggccaat acgcccgcgt ttcttccttt tccccacccc accccccaag
     4381 ttcgggtgaa ggcccagggc tcgcagccaa cgtcggggcg gcaggccctg ccatagccac
     4441 tggccccgtg ggttagggac ggggtccccc atggggaatg gtttatggtt cgtgggggtt
     4501 attattttgg gcgttgcgtg gggtcaggtc cacgactgga ctgagcagac agacccatgg
     4561 tttttggatg gcctgggcat ggaccgcatg tactggcgcg acacgaacac cgggcgtctg
     4621 tggctgccaa acacccccga cccccaaaaa ccaccgcgcg gatttctggc gtgccaagct
     4681 agtcgaccaa ttctcatgtt tgacagctta tcatcgcaga tccgggcaac gttgttgcca
     4741 ttgctgcagg cgcagaactg gtaggtatgg aagatctata cattgaatca atattggcaa
     4801 ttagccatat tagtcattgg ttatatagca taaatcaata ttggctattg gccattgcat
     4861 acgttgtatc tatatcataa tatgtacatt tatattggct catgtccaat atgaccgcca
     4921 tgttgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat
     4981 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
     5041 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
     5101 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
     5161 catcaagtgt atcatatgcc aagtccgccc cctattgacg tcaatgacgg taaatggccc
     5221 gcctggcatt atgcccagta catgacctta cgggactttc ctacttggca gtacatctac
     5281 gtattagtca tcgctattac catggtgatg cggttttggc agtacaccaa tgggcgtgga
     5341 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
     5401 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta ataaccccgc cccgttgacg
     5461 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac
     5521 cgtcagatct ctagaagctg ggtaccagct gctagcgcca ccatggccgt catggcgccc
     5581 cgaaccctcg tcctgctact ctcgggggcc ctggccctga cccagacctg ggcgggctcc
     5641 gactacaagg acgatgatga caagggagga tccaagcccc acccagcaga ggggcagtgg
     5701 cgggcagtgg acgtggtcct agactgtttc ctggtgaagg acggtgcgca ccgtggagct
     5761 ctcgccagca gtgaggacag ggcaagggcc tcccttgtgc tgaagcaggt gccagtgctg
     5821 gacgatggct ccctggagga cttcaccgat ttccaagggg gcacactggc ccaagatgac
     5881 ccacctatta tctttgaggc ctcagtggac ctggtccaga ttccccaggc cgaggccttg
     5941 ctccatgctg actgcagtgg gaaggaggtg acctgtgaga tctcccgcta ctttctccag
     6001 atgacagaga ccactgttaa gacagcagct tggttcatgg ccaacgtgca ggtctctgga
     6061 gggggaccta gcatctcctt ggtgatgaag actcccaggg tcgccaagaa tgaggtgctc
     6121 tggcacccaa cgctgaactt gccactgagc ccccagggga ctgtgcgaac tgcagtggag
     6181 ttccaggtga tgacacagac ccaatccctg agcttcctgc tggggtcctc agcctccttg
     6241 gactgtggct tctccatggc accgggcttg gacctcatca gtgtggagtg gcgactgcag
     6301 cacaagggca ggggtcagtt ggtgtacagc tggaccgcag ggcaggggca ggctgtgcgg
     6361 aagggcgcta ccctggagcc tgcacaactg ggcatggcca gggatgcctc cctcaccctg
     6421 cccggcctca ctatacagga cgaggggacc tacatttgcc agatcaccac ctctctgtac
     6481 cgagctcagc agatcatcca gctcaacatc caagcttccc ctaaagtacg actgagcttg
     6541 gcaaacgaag ctctgctgcc caccctcatc tgcgacattg ctggctatta ccctctggat
     6601 gtggtggtga cgtggacccg agaggagctg ggtggatccc cagcccaagt ctctggtgcc
     6661 tccttctcca gcctcaggca aagcgtggca ggcacctaca gcatctcctc ctctctcacc
     6721 gcagaacctg gctctgcagg tgccacttac acctgccagg tcacacacat ctctctggag
     6781 gagccccttg gggccagcac ccaggttgtc ccaccagagc ggagaacagc cttgggagtc
     6841 atctttgcca gcagtctctt ccttcttgca ctgatgttcc tggggcttca gagacggcaa
     6901 gcacctacag gacttgggct gcttcaggct gaacgctggg agaccacttc ctgtgctgac
     6961 acacagagct cccatctcca tgaagaccgc acagcgcgtg taagccagcc cagctaatag
     7021 ctcgaggccg gcaaggccgg atccagacat gataagatac attgatgagt ttggacaaac
     7081 cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt
     7141 atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca ttcattttat
     7201 gtttcaggtt cagggggagg tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg
     7261 tggtatggct gattatgatc cggctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc
     7321 tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga
     7381 caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc catgaggtcg
     7441 actctagagg atcgatcccc gccccggacg aactaaacct gactacgaca tctctgcccc
     7501 ttcttcgcgg ggcagtgcat gtaatccctt cagttggttg gtacaacttg ccaactgggc
     7561 cctgttccac atgtgacacg gggggggacc aaacacaaag gggttctctg actgtagttg
     7621 acatccttat aaatggatgt gcacatttgc caacactgag tggctttcat cctggagcag
     7681 actttgcagt ctgtggactg caacacaaca ttgcctttat gtgtaactct tggctgaagc
     7741 tcttacacca atgctggggg acatgtacct cccaggggcc caggaagact acgggaggct
     7801 acaccaacgt caatcagagg ggcctgtgta gctaccgata agcggaccct caagagggca
     7861 ttagcaatag tgtttataag gcccccttgt taaccctaaa cgggtagcat atgcttcccg
     7921 ggtagtagta tatactatcc agactaaccc taattcaata gcatatgtta cccaacggga
     7981 agcatatgct atcgaattag ggttagtaaa agggtcctaa ggaacagcga tatctcccac
     8041 cccatgagct gtcacggttt tatttacatg gggtcaggat tccacgaggg tagtgaacca
     8101 ttttagtcac aagggcagtg gctgaagatc aaggagcggg cagtgaactc tcctgaatct
     8161 tcgcctgctt cttcattctc cttcgtttag ctaatagaat aactgctgag ttgtgaacag
     8221 taaggtgtat gtgaggtgct cgaaaacaag gtttcaggtg acgcccccag aataaaattt
     8281 ggacgggggg ttcagtggtg gcattgtgct atgacaccaa tataaccctc acaaacccct
     8341 tgggcaataa atactagtgt aggaatgaaa cattctgaat atctttaaca atagaaatcc
     8401 atggggtggg gacaagccgt aaagactgga tgtccatctc acacgaattt atggctatgg
     8461 gcaacacata atcctagtgc aatatgatac tggggttatt aagatgtgtc ccaggcaggg
     8521 accaagacag gtgaaccatg ttgttacact ctatttgtaa caaggggaaa gagagtggac
     8581 gccgacagca gcggactcca ctggttgtct ctaacacccc cgaaaattaa acggggctcc
     8641 acgccaatgg ggcccataaa caaagacaag tggccactct tttttttgaa attgtggagt
     8701 gggggcacgc gtcagccccc acacgccgcc ctgcggtttt ggactgtaaa ataagggtgt
     8761 aataacttgg ctgattgtaa ccccgctaac cactgcggtc aaaccacttg cccacaaaac
     8821 cactaatggc accccgggga atacctgcat aagtaggtgg gcgggccaag ataggggcgc
     8881 gattgctgcg atctggagga caaattacac acacttgcgc ctgagcgcca agcacagggt
     8941 tgttggtcct catattcacg aggtcgctga gagcacggtg ggctaatgtt gccatgggta
     9001 gcatatacta cccaaatatc tggatagcat atgctatcct aatctatatc tgggtagcat
     9061 aggctatcct aatctatatc tgggtagcat atgctatcct aatctatatc tgggtagtat
     9121 atgctatcct aatttatatc tgggtagcat aggctatcct aatctatatc tgggtagcat
     9181 atgctatcct aatctatatc tgggtagtat atgctatcct aatctgtatc cgggtagcat
     9241 atgctatcct aatagagatt agggtagtat atgctatcct aatttatatc tgggtagcat
     9301 atactaccca aatatctgga tagcatatgc tatcctaatc tatatctggg tagcatatgc
     9361 tatcctaatc tatatctggg tagcataggc tatcctaatc tatatctggg tagcatatgc
     9421 tatcctaatc tatatctggg tagtatatgc tatcctaatt tatatctggg tagcataggc
     9481 tatcctaatc tatatctggg tagcatatgc tatcctaatc tatatctggg tagtatatgc
     9541 tatcctaatc tgtatccggg tagcatatgc tatcctcatg catatacagt cagcatatga
     9601 tacccagtag tagagtggga gtgctatcct ttgcatatgc cgccacctcc caagggggcg
     9661 tgaattttcg ctgcttgtcc ttttcctgct ggttgctccc attcttaggt gaatttaagg
     9721 aggccaggct aaagccgtcg catgtctgat tgctcaccag gtaaatgt
//
