LOCUS       sequence-270561-        7948 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of myc-tagged HLA-A*024:02.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 7948)
  AUTHORS   McShan AC, Devlin CA, Overall SA, Park J, Toor JS, Moschidi D, 
            Flores-Solis D, Choi H, Tripathi S, Procko E, Sgourakis NG
  TITLE     Molecular determinants of chaperone interactions on MHC-I for 
            folding and antigen repertoire selection.
  JOURNAL   Proc Natl Acad Sci U S A. 2019 Dec 17;116(51):25602-25613. doi: 
            10.1073/pnas.1915562116. Epub 2019 Dec 3.
  PUBMED    31796585
REFERENCE   2  (bases 1 to 7948)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1915562116"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2019-12-17- 17"; volume: "116"; issue: "51"; pages: 
            "25602-25613"
COMMENT     Sequence Label: pCEP4-myc-HLA-A24
FEATURES             Location/Qualifiers
     source          1..7948
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(228..246)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        314..418
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(419..487,488..1279)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(637..656)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1450..2038
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1939..1958
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        2469..2614
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             2657..3694
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(3692..3711)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    3736..3784
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        4438..4817
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4818..5021
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4972..4992
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5018..5042
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             5124..5153
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     polyA_signal    6229..6363
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6253..6272)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6307..6326
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(6506..6528)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 gaacatcctc aagatttgcg tcctgagcct caagccaggc ctcaaattcc tcgtccccct
       61 ttttgctgga cggtagggat ggggattctc gggacccctc ctcttcctct tcaaggtcac
      121 cagacagaga tgctactggg gcaacggaag aaaagctggg tgcggcctgt gaggatcagc
      181 ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg
      241 cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt
      301 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
      361 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
      421 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
      481 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
      541 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
      601 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
      661 tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
      721 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
      781 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
      841 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
      901 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
      961 tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     1021 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     1081 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     1141 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     1201 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     1261 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     1321 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     1381 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     1441 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     1501 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     1561 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg
     1621 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     1681 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     1741 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     1801 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     1861 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     1921 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     1981 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     2041 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttgaa gctgtccctg
     2101 atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc
     2161 ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa
     2221 gacgtagccc agcgcgtcgg ccccgagatg cgccgcgtgc ggctgctgga gatggcggac
     2281 gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga
     2341 ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccctgc ttcatccccg
     2401 tggcccgttg ctcgcgtttg ctggcggtgt ccccggaaga aatatatttg catgtcttta
     2461 gttctatgat gacacaaacc ccgcccagcg tcttgtcatt ggcgaattcg aacacgcaga
     2521 tgcagtcggg gcggcgcggt ccgaggtcca cttcgcatat taaggtgacg cgtgtggcct
     2581 cgaacaccga gcgaccctgc agcgacccgc ttaacagcgt caacagcgtg ccgcagatcc
     2641 cggggggcaa tgagatatga aaaagcctga actcaccgcg acgtctgtcg agaagtttct
     2701 gatcgaaaag ttcgacagcg tctccgacct gatgcagctc tcggagggcg aagaatctcg
     2761 tgctttcagc ttcgatgtag gagggcgtgg atatgtcctg cgggtaaata gctgcgccga
     2821 tggtttctac aaagatcgtt atgtttatcg gcactttgca tcggccgcgc tcccgattcc
     2881 ggaagtgctt gacattgggg aattcagcga gagcctgacc tattgcatct cccgccgtgc
     2941 acagggtgtc acgttgcaag acctgcctga aaccgaactg cccgctgttc tgcagccggt
     3001 cgcggaggcc atggatgcga tcgctgcggc cgatcttagc cagacgagcg ggttcggccc
     3061 attcggaccg caaggaatcg gtcaatacac tacatggcgt gatttcatat gcgcgattgc
     3121 tgatccccat gtgtatcact ggcaaactgt gatggacgac accgtcagtg cgtccgtcgc
     3181 gcaggctctc gatgagctga tgctttgggc cgaggactgc cccgaagtcc ggcacctcgt
     3241 gcacgcggat ttcggctcca acaatgtcct gacggacaat ggccgcataa cagcggtcat
     3301 tgactggagc gaggcgatgt tcggggattc ccaatacgag gtcgccaaca tcttcttctg
     3361 gaggccgtgg ttggcttgta tggagcagca gacgcgctac ttcgagcgga ggcatccgga
     3421 gcttgcagga tcgccgcggc tccgggcgta tatgctccgc attggtcttg accaactcta
     3481 tcagagcttg gttgacggca atttcgatga tgcagcttgg gcgcagggtc gatgcgacgc
     3541 aatcgtccga tccggagccg ggactgtcgg gcgtacacaa atcgcccgca gaagcgcggc
     3601 cgtctggacc gatggctgtg tagaagtact cgccgatagt ggaaaccgac gccccagcac
     3661 tcgtccggat cgggagatgg gggaggctaa ctgaaacacg gaaggagaca ataccggaag
     3721 gaacccgcgc tatgacggca ataaaaagac agaataaaac gcacgggtgt tgggtcgttt
     3781 gttcataaac gcggggttcg gtcccagggc tggcactctg tcgatacccc accgagaccc
     3841 cattggggcc aatacgcccg cgtttcttcc ttttccccac cccacccccc aagttcgggt
     3901 gaaggcccag ggctcgcagc caacgtcggg gcggcaggcc ctgccatagc cactggcccc
     3961 gtgggttagg gacggggtcc cccatgggga atggtttatg gttcgtgggg gttattattt
     4021 tgggcgttgc gtggggtcag gtccacgact ggactgagca gacagaccca tggtttttgg
     4081 atggcctggg catggaccgc atgtactggc gcgacacgaa caccgggcgt ctgtggctgc
     4141 caaacacccc cgacccccaa aaaccaccgc gcggatttct ggcgtgccaa gctagtcgac
     4201 caattctcat gtttgacagc ttatcatcgc agatccgggc aacgttgttg ccattgctgc
     4261 aggcgcagaa ctggtaggta tggaagatct atacattgaa tcaatattgg caattagcca
     4321 tattagtcat tggttatata gcataaatca atattggcta ttggccattg catacgttgt
     4381 atctatatca taatatgtac atttatattg gctcatgtcc aatatgaccg ccatgttgac
     4441 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
     4501 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
     4561 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
     4621 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
     4681 tgtatcatat gccaagtccg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
     4741 attatgccca gtacatgacc ttacgggact ttcctacttg gcagtacatc tacgtattag
     4801 tcatcgctat taccatggtg atgcggtttt ggcagtacac caatgggcgt ggatagcggt
     4861 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
     4921 accaaaatca acgggacttt ccaaaatgtc gtaataaccc cgccccgttg acgcaaatgg
     4981 gcggtaggcg tgtacggtgg gaggtctata taagcagagc tcgtttagtg aaccgtcaga
     5041 tctctagaag ctgggtacca gctgctagcg ccaccatgaa gaccatcatc gccctgagct
     5101 acatcttctg cctggtgttc gccgagcaga aattgatcag cgaggaggac ctgggcagcc
     5161 ctggaggcag ttcaggaggc ggatctcatt ctatgagata tttctccaca tccgtgtccc
     5221 ggcccggccg cggggagccc cgcttcatcg ccgtgggcta cgtggacgac acgcagttcg
     5281 tgcggttcga cagcgacgcc gcgagccaga ggatggagcc gcgggcgccg tggatagagc
     5341 aggaggggcc ggagtattgg gacgaggaga cagggaaagt gaaggcccac tcacagactg
     5401 accgagagaa cctgcggatc gcgctccgct actacaacca gagcgaggcc ggttctcaca
     5461 ccctccagat gatgtttggc tgcgacgtgg ggtcggacgg gcgcttcctc cgcgggtacc
     5521 accagtacgc ctacgacggc aaggattaca tcgccctgaa agaggacctg cgctcttgga
     5581 ccgcggcgga catggcggct cagatcacca agcgcaagtg ggaggcggcc catgtggcgg
     5641 agcagcagag agcctacctg gagggcacgt gcgtggacgg gctccgcaga tacctggaga
     5701 acgggaagga gacgctgcag cgcacggacc cccccaagac acatatgacc caccacccca
     5761 tctctgacca tgaggccact ctgagatgct gggccctggg cttctaccct gcggagatca
     5821 cactgacctg gcagcgggat ggggaggacc agacccagga cacggagctt gtggagacca
     5881 ggcctgcagg ggatggaacc ttccagaagt gggcagctgt ggtggtacct tctggagagg
     5941 agcagagata cacctgccat gtgcagcatg agggtctgcc caagcccctc accctgagat
     6001 gggagccatc ttcccagccc acggtgccca tcgtaggcat aatagctggc cttgtgctcc
     6061 ttggggccgt aataactggg gcagttgtcg ccgccgtgat gtggcggaga aacagtagtg
     6121 atcgaaaagg cggtagctat tcccaagcag cgagtagcga ttctgcccag gggagtgacg
     6181 tgtcactgac ggcctgtaaa gtttgataac tcgaggccgg caaggccgga tccagacatg
     6241 ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt
     6301 atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa
     6361 gttaacaaca acaattgcat tcattttatg tttcaggttc agggggaggt gtgggaggtt
     6421 ttttaaagca agtaaaacct ctacaaatgt ggtatggctg attatgatcc ggctgcctcg
     6481 cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag
     6541 cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg
     6601 gcgggtgtcg gggcgcagcc atgaggtcga ctctagagga tcgatccccg ccccggacga
     6661 actaaacctg actacgacat ctctgcccct tcttcgcggg gcagtgcatg taatcccttc
     6721 agttggttgg tacaacttgc caactgggcc ctgttccaca tgtgacacgg ggggggacca
     6781 aacacaaagg ggttctctga ctgtagttga catccttata aatggatgtg cacatttgcc
     6841 aacactgagt ggctttcatc ctggagcaga ctttgcagtc tgtggactgc aacacaacat
     6901 tgcctttatg tgtaactctt ggctgaagct cttacaccaa tgctggggga catgtacctc
     6961 ccaggggccc aggaagacta cgggaggcta caccaacgtc aatcagaggg gcctgtgtag
     7021 ctaccgataa gcggaccctc aagagggcat tagcaatagt gtttataagg cccccttgtt
     7081 aaccctaaac gggtagcata tgcttcccgg gtagtagtat atactatcca gactaaccct
     7141 aattcaatag catatgttac ccaacgggaa gcatatgcta tcgaattagg gttagtaaaa
     7201 gggtcctaag gaacagcgat atctcccacc ccatgagctg tcacggtttt atttacatgg
     7261 ggtcaggatt ccacgagggt agtgaaccat tttagtcaca agggcagtgg ctgaagatca
     7321 aggagcgggc agtgaactct cctgaatctt cgcctgcttc ttcattctcc ttcgtttagc
     7381 taatagaata actgctgagt tgtgaacagt aaggtgtatg tgaggtgctc gaaaacaagg
     7441 tttcaggtga cgcccccaga ataaaatttg gacggggggt tcagtggtgg cattgtgcta
     7501 tgacaccaat ataaccctca caaacccctt gggcaataaa tactagtgta ggaatgaaac
     7561 attctgaata tctttaacaa tagaaatcca tggggtgggg acaagccgta aagactggat
     7621 gtccatctca cacgaattta tggctatggg caacacataa tcctagtgca atatgatact
     7681 ggggttatta agatgtgtcc caggcaggga ccaagacagg tgaaccatgt tgttacactc
     7741 tatttgtaac aaggggaaag agagtggacg ccgacagcag cggactccac tggttgtctc
     7801 taacaccccc gaaaattaaa cggggctcca cgccaatggg gcccataaac aaagacaagt
     7861 ggccactctt ttttttgaaa ttgtggagtg ggggcacgcg tcagccccca cacgccgccc
     7921 tgcggttttg gactgtaaaa taagggtg
//
