LOCUS       sequence-270564-       10144 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of myc-tagged HLA-B*57:01.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 10144)
  AUTHORS   McShan AC, Devlin CA, Overall SA, Park J, Toor JS, Moschidi D, 
            Flores-Solis D, Choi H, Tripathi S, Procko E, Sgourakis NG
  TITLE     Molecular determinants of chaperone interactions on MHC-I for 
            folding and antigen repertoire selection.
  JOURNAL   Proc Natl Acad Sci U S A. 2019 Dec 17;116(51):25602-25613. doi: 
            10.1073/pnas.1915562116. Epub 2019 Dec 3.
  PUBMED    31796585
REFERENCE   2  (bases 1 to 10144)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1915562116"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2019-12-17- 17"; volume: "116"; issue: "51"; pages: 
            "25602-25613"
COMMENT     Sequence Label: pCEP4-myc-HLA-B57
FEATURES             Location/Qualifiers
     source          1..10144
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(247..265)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        333..437
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(438..506,507..1298)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(656..675)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1469..2057
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1958..1977
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        2488..2633
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             2676..3713
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(3711..3730)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    3755..3803
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        4457..4836
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4837..5040
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4991..5011
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5037..5061
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             5143..5172
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     polyA_signal    6239..6373
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6263..6282)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6317..6336
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(6516..6538)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      7098..8887
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
ORIGIN
        1 agcccggaaa actcccgctg aacatcctca agatttgcgt cctgagcctc aagccaggcc
       61 tcaaattcct cgtccccctt tttgctggac ggtagggatg gggattctcg ggacccctcc
      121 tcttcctctt caaggtcacc agacagagat gctactgggg caacggaaga aaagctgggt
      181 gcggcctgtg aggatcagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac
      241 gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt
      301 agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
      361 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
      421 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
      481 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
      541 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
      601 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
      661 gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact
      721 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
      781 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
      841 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
      901 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
      961 agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     1021 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     1081 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     1141 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     1201 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     1261 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     1321 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     1381 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     1441 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     1501 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     1561 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc
     1621 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     1681 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     1741 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     1801 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     1861 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     1921 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     1981 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     2041 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     2101 ggccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg
     2161 cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc
     2221 gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cccgagatgc gccgcgtgcg
     2281 gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac
     2341 agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg
     2401 ccgccctgct tcatccccgt ggcccgttgc tcgcgtttgc tggcggtgtc cccggaagaa
     2461 atatatttgc atgtctttag ttctatgatg acacaaaccc cgcccagcgt cttgtcattg
     2521 gcgaattcga acacgcagat gcagtcgggg cggcgcggtc cgaggtccac ttcgcatatt
     2581 aaggtgacgc gtgtggcctc gaacaccgag cgaccctgca gcgacccgct taacagcgtc
     2641 aacagcgtgc cgcagatccc ggggggcaat gagatatgaa aaagcctgaa ctcaccgcga
     2701 cgtctgtcga gaagtttctg atcgaaaagt tcgacagcgt ctccgacctg atgcagctct
     2761 cggagggcga agaatctcgt gctttcagct tcgatgtagg agggcgtgga tatgtcctgc
     2821 gggtaaatag ctgcgccgat ggtttctaca aagatcgtta tgtttatcgg cactttgcat
     2881 cggccgcgct cccgattccg gaagtgcttg acattgggga attcagcgag agcctgacct
     2941 attgcatctc ccgccgtgca cagggtgtca cgttgcaaga cctgcctgaa accgaactgc
     3001 ccgctgttct gcagccggtc gcggaggcca tggatgcgat cgctgcggcc gatcttagcc
     3061 agacgagcgg gttcggccca ttcggaccgc aaggaatcgg tcaatacact acatggcgtg
     3121 atttcatatg cgcgattgct gatccccatg tgtatcactg gcaaactgtg atggacgaca
     3181 ccgtcagtgc gtccgtcgcg caggctctcg atgagctgat gctttgggcc gaggactgcc
     3241 ccgaagtccg gcacctcgtg cacgcggatt tcggctccaa caatgtcctg acggacaatg
     3301 gccgcataac agcggtcatt gactggagcg aggcgatgtt cggggattcc caatacgagg
     3361 tcgccaacat cttcttctgg aggccgtggt tggcttgtat ggagcagcag acgcgctact
     3421 tcgagcggag gcatccggag cttgcaggat cgccgcggct ccgggcgtat atgctccgca
     3481 ttggtcttga ccaactctat cagagcttgg ttgacggcaa tttcgatgat gcagcttggg
     3541 cgcagggtcg atgcgacgca atcgtccgat ccggagccgg gactgtcggg cgtacacaaa
     3601 tcgcccgcag aagcgcggcc gtctggaccg atggctgtgt agaagtactc gccgatagtg
     3661 gaaaccgacg ccccagcact cgtccggatc gggagatggg ggaggctaac tgaaacacgg
     3721 aaggagacaa taccggaagg aacccgcgct atgacggcaa taaaaagaca gaataaaacg
     3781 cacgggtgtt gggtcgtttg ttcataaacg cggggttcgg tcccagggct ggcactctgt
     3841 cgatacccca ccgagacccc attggggcca atacgcccgc gtttcttcct tttccccacc
     3901 ccacccccca agttcgggtg aaggcccagg gctcgcagcc aacgtcgggg cggcaggccc
     3961 tgccatagcc actggccccg tgggttaggg acggggtccc ccatggggaa tggtttatgg
     4021 ttcgtggggg ttattatttt gggcgttgcg tggggtcagg tccacgactg gactgagcag
     4081 acagacccat ggtttttgga tggcctgggc atggaccgca tgtactggcg cgacacgaac
     4141 accgggcgtc tgtggctgcc aaacaccccc gacccccaaa aaccaccgcg cggatttctg
     4201 gcgtgccaag ctagtcgacc aattctcatg tttgacagct tatcatcgca gatccgggca
     4261 acgttgttgc cattgctgca ggcgcagaac tggtaggtat ggaagatcta tacattgaat
     4321 caatattggc aattagccat attagtcatt ggttatatag cataaatcaa tattggctat
     4381 tggccattgc atacgttgta tctatatcat aatatgtaca tttatattgg ctcatgtcca
     4441 atatgaccgc catgttgaca ttgattattg actagttatt aatagtaatc aattacgggg
     4501 tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg
     4561 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata
     4621 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc
     4681 cacttggcag tacatcaagt gtatcatatg ccaagtccgc cccctattga cgtcaatgac
     4741 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tacgggactt tcctacttgg
     4801 cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg gcagtacacc
     4861 aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc
     4921 aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taataacccc
     4981 gccccgttga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct
     5041 cgtttagtga accgtcagat ctctagaagc tgggtaccag ctgctagcgc caccatgaag
     5101 accatcatcg ccctgagcta catcttctgc ctggtgttcg ccgagcagaa attgatcagc
     5161 gaggaggacc tgggcagccc tggaggcagt tcaggaggcg gatctcattc tatgagatat
     5221 ttctacaccg ccatgtcccg gcccggccgc ggggagcccc gcttcatcgc agtgggctac
     5281 gtggacgaca cccagttcgt gaggttcgac agcgacgccg cgagtccgag gatggcgccc
     5341 cgggcgccat ggatagagca ggaggggccg gagtattggg acggggagac acggaacatg
     5401 aaggcctccg cgcagactta ccgagagaac ctgcggatcg cgctccgcta ctacaaccag
     5461 agcgaggccg ggtctcacat catccaggtg atgtatggct gcgacgtggg gccggacggg
     5521 cgcctcctac gcgggcatga ccagtccgcc tacgacggca aggattacat cgccctgaac
     5581 gaggacctga gctcctggac cgcggcggac acggcggctc agatcaccca gcgcaagtgg
     5641 gaggcggccc gtgtggcgga gcagctgaga gcctacctgg agggcctgtg cgtggagtgg
     5701 ctccgcagat acctggagaa cgggaaggag acgctgcagc gcgcggaccc cccaaagaca
     5761 catgtgaccc accaccccat ctctgaccat gaggccaccc tgaggtgctg ggccctgggc
     5821 ttctaccctg cggagatcac actgacctgg cagcgggatg gcgaggacca aactcaggac
     5881 accgagcttg tggagaccag accagcagga gatagaacct tccagaagtg ggcagctgtg
     5941 gtggtgcctt ctggagaaga gcagagatac acatgccatg tacagcatga ggggctgcca
     6001 aagcccctca ccctgagatg ggagccatct tcccaatcca ctgttcccat cgtcggtata
     6061 gttgctggcc tcgcagtctt ggcggtagta gtcattggag ctgtagtggc agcagtgatg
     6121 tgcagacgca agagttccgg cggcaaggga ggctcctatt ctcaagctgc ttgctccgac
     6181 tcagcacaag gcagtgatgt ctcattgact gcatgataac tcgaggccgg caaggccgga
     6241 tccagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa
     6301 aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg
     6361 caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc agggggaggt
     6421 gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtatggctg attatgatcc
     6481 ggctgcctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag
     6541 acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca
     6601 gcgggtgttg gcgggtgtcg gggcgcagcc atgaggtcga ctctagagga tcgatccccg
     6661 ccccggacga actaaacctg actacgacat ctctgcccct tcttcgcggg gcagtgcatg
     6721 taatcccttc agttggttgg tacaacttgc caactgggcc ctgttccaca tgtgacacgg
     6781 ggggggacca aacacaaagg ggttctctga ctgtagttga catccttata aatggatgtg
     6841 cacatttgcc aacactgagt ggctttcatc ctggagcaga ctttgcagtc tgtggactgc
     6901 aacacaacat tgcctttatg tgtaactctt ggctgaagct cttacaccaa tgctggggga
     6961 catgtacctc ccaggggccc aggaagacta cgggaggcta caccaacgtc aatcagaggg
     7021 gcctgtgtag ctaccgataa gcggaccctc aagagggcat tagcaatagt gtttataagg
     7081 cccccttgtt aaccctaaac gggtagcata tgcttcccgg gtagtagtat atactatcca
     7141 gactaaccct aattcaatag catatgttac ccaacgggaa gcatatgcta tcgaattagg
     7201 gttagtaaaa gggtcctaag gaacagcgat atctcccacc ccatgagctg tcacggtttt
     7261 atttacatgg ggtcaggatt ccacgagggt agtgaaccat tttagtcaca agggcagtgg
     7321 ctgaagatca aggagcgggc agtgaactct cctgaatctt cgcctgcttc ttcattctcc
     7381 ttcgtttagc taatagaata actgctgagt tgtgaacagt aaggtgtatg tgaggtgctc
     7441 gaaaacaagg tttcaggtga cgcccccaga ataaaatttg gacggggggt tcagtggtgg
     7501 cattgtgcta tgacaccaat ataaccctca caaacccctt gggcaataaa tactagtgta
     7561 ggaatgaaac attctgaata tctttaacaa tagaaatcca tggggtgggg acaagccgta
     7621 aagactggat gtccatctca cacgaattta tggctatggg caacacataa tcctagtgca
     7681 atatgatact ggggttatta agatgtgtcc caggcaggga ccaagacagg tgaaccatgt
     7741 tgttacactc tatttgtaac aaggggaaag agagtggacg ccgacagcag cggactccac
     7801 tggttgtctc taacaccccc gaaaattaaa cggggctcca cgccaatggg gcccataaac
     7861 aaagacaagt ggccactctt ttttttgaaa ttgtggagtg ggggcacgcg tcagccccca
     7921 cacgccgccc tgcggttttg gactgtaaaa taagggtgta ataacttggc tgattgtaac
     7981 cccgctaacc actgcggtca aaccacttgc ccacaaaacc actaatggca ccccggggaa
     8041 tacctgcata agtaggtggg cgggccaaga taggggcgcg attgctgcga tctggaggac
     8101 aaattacaca cacttgcgcc tgagcgccaa gcacagggtt gttggtcctc atattcacga
     8161 ggtcgctgag agcacggtgg gctaatgttg ccatgggtag catatactac ccaaatatct
     8221 ggatagcata tgctatccta atctatatct gggtagcata ggctatccta atctatatct
     8281 gggtagcata tgctatccta atctatatct gggtagtata tgctatccta atttatatct
     8341 gggtagcata ggctatccta atctatatct gggtagcata tgctatccta atctatatct
     8401 gggtagtata tgctatccta atctgtatcc gggtagcata tgctatccta atagagatta
     8461 gggtagtata tgctatccta atttatatct gggtagcata tactacccaa atatctggat
     8521 agcatatgct atcctaatct atatctgggt agcatatgct atcctaatct atatctgggt
     8581 agcataggct atcctaatct atatctgggt agcatatgct atcctaatct atatctgggt
     8641 agtatatgct atcctaattt atatctgggt agcataggct atcctaatct atatctgggt
     8701 agcatatgct atcctaatct atatctgggt agtatatgct atcctaatct gtatccgggt
     8761 agcatatgct atcctcatgc atatacagtc agcatatgat acccagtagt agagtgggag
     8821 tgctatcctt tgcatatgcc gccacctccc aagggggcgt gaattttcgc tgcttgtcct
     8881 tttcctgctg gttgctccca ttcttaggtg aatttaagga ggccaggcta aagccgtcgc
     8941 atgtctgatt gctcaccagg taaatgtcgc taatgttttc caacgcgaga aggtgttgag
     9001 cgcggagctg agtgacgtga caacatgggt atgcccaatt gccccatgtt gggaggacga
     9061 aaatggtgac aagacagatg gccagaaata caccaacagc acgcatgatg tctactgggg
     9121 atttattctt tagtgcgggg gaatacacgg cttttaatac gattgagggc gtctcctaac
     9181 aagttacatc actcctgccc ttcctcaccc tcatctccat cacctccttc atctccgtca
     9241 tctccgtcat caccctccgc ggcagcccct tccaccatag gtggaaacca gggaggcaaa
     9301 tctactccat cgtcaaagct gcacacagtc accctgatat tgcaggtagg agcgggcttt
     9361 gtcataacaa ggtccttaat cgcatccttc aaaacctcag caaatatatg agtttgtaaa
     9421 aagaccatga aataacagac aatggactcc cttagcgggc caggttgtgg gccgggtcca
     9481 ggggccattc caaaggggag acgactcaat ggtgtaagac gacattgtgg aatagcaagg
     9541 gcagttcctc gccttaggtt gtaaagggag gtcttactac ctccatatac gaacacaccg
     9601 gcgacccaag ttccttcgtc ggtagtcctt tctacgtgac tcctagccag gagagctctt
     9661 aaaccttctg caatgttctc aaatttcggg ttggaacctc cttgaccacg atgctttcca
     9721 aaccaccctc cttttttgcg cctgcctcca tcaccctgac cccggggtcc agtgcttggg
     9781 ccttctcctg ggtcatctgc ggggccctgc tctatcgctc ccgggggcac gtcaggctca
     9841 ccatctgggc caccttcttg gtggtattca aaataatcgg cttcccctac agggtggaaa
     9901 aatggccttc tacctggagg gggcctgcgc ggtggagacc cggatgatga tgactgacta
     9961 ctgggactcc tgggcctctt ttctccacgt ccacgacctc tccccctggc tctttcacga
    10021 cttccccccc tggctctttc acgtcctcta ccccggcggc ctccactacc tcctcgaccc
    10081 cggcctccac tacctcctcg accccggcct ccactgcctc ctcgaccccg gcctccacct
    10141 cctg
//
