LOCUS       sequence-270566-       13631 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PPI (BiFC/TriFC) assay vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13631)
  TITLE     Lewin Lab Plasmids
REFERENCE   2  (bases 1 to 13631)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pRDAX-502    N-Tag GFP[1] L20 Piezo1 mCherry
FEATURES             Location/Qualifiers
     source          1..13631
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        153..532
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        533..736
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     687..707
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     733..757
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          872..1004
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     regulatory      1019..1028
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    join(8799..9373,9374..9376,9377..9385)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(8966..8983)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     9193..9212
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             9386..10096
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(9531..9549)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     9799..9818
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     intron          10142..10274
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     misc_feature    10283..10871
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(10336..10356)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10754..10765)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(10898..10915)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    10904..11015
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(11111..11131)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(11111..11129)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(11150..11166)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11150..11166)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11163..11185)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     complement(11268..11287)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    11333..11388
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(11387..11406)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     rep_origin      complement(11767..12355)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11847..11866)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12526..13317,13318..13386))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13149..13168
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13387..13491)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     13559..13577
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gcctgcctcc aggcctcaat attggccatt agccatatta ttcattggtt atatagcata
       61 aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata tgtacattta
      121 tattggctca tgtccaatat gaccgccatg ttggcattga ttattgacta gttattaata
      181 gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact
      241 tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat
      301 gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta
      361 tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa gtccgccccc
      421 tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttacg
      481 ggactttcct acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg
      541 gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct
      601 ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa
      661 atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac ggtgggaggt
      721 ctatataagc agagctcgtt tagtgaaccg tcagatcact agaagcttta ttgcggtagt
      781 ttatcacagt taaattgcta acgcagtcag tgcttctgac acaacagtct cgaacttaag
      841 ctgcagaagt tggtcgtgag gcactgggca ggtaagtatc aaggttacaa gacaggttta
      901 aggagaccaa tagaaactgg gcttgtcgag acagagaaga ctcttgcgtt tctgataggc
      961 acctattggt cttactgaca tccactttgc ctttctctcc acaggtgtcc actcccaggc
     1021 caccatggat ctgcctgatg accactacct gagcacacag accatcctct ccaaggacct
     1081 taacgggagt cctggcggtg gatccggagg ctctgggagc tctgccagcg gtggcagcac
     1141 ctccgagccg cacgtgctgg gcgccgggct ctactggctg ttgctgccct gcacgctcct
     1201 ggcggcctcc ctgttacgct tcaatgctct ctcgctggtc tatttgctgt ttctactgct
     1261 gctgccctgg cttccaggcc cctcaagaca cagcatacca ggtcacacag gtcgcctgct
     1321 ccgtgcacta ctctgcctca gcctcctctt cctggtggcc caccttgcct ttcagatatg
     1381 cctacacacc gtgcctcacc tggaccagtt tctgggacaa aacggtagcc tttgggtgaa
     1441 ggtgtctcaa cacatagggg ttacgaggct ggacctgaag gacatcttta acaccaccag
     1501 gctggtagca cctgacctgg gagtgctgct ggcgtcctcc ctttgccttg gcctctgtgg
     1561 acgcctcacg aggaaagccg ggcagagtcg gcgcacccag gagctgcagg atgatgacga
     1621 tgacgatgat gacgacgatg aagacataga tgctgcccca gccgtggggc tgaagggagc
     1681 ccctgccctg gcaaccaaac gcaggctgtg gctggcctcc cgcttccggg tcacggccca
     1741 ctggctgctg atgacctctg gacggacgct ggtcattgtg ttgctggccc tggcaggcat
     1801 agcccaccct tcggccttct ccagcatcta cctggtggtg ttcctggcca tctgcacctg
     1861 gtggtcctgt cactttcctc tcagccccct gggctttaac accctctgtg tcatggtgag
     1921 ctgttttggt gccggccatc tcatttgcct atactgctat cagacaccat ttatccagga
     1981 catgttaccc cctgggaaca tctgggccag gctatttggt ctcaagaact tcgtagacct
     2041 ccctaactac tccagcccca acgccctggt actcaacact aagcacgcct ggcccatcta
     2101 tgtgagtccc ggaatcctgc tgctgctata ttacacagcc acctctctcc tgaagctcca
     2161 caagagctgt ccctcagagc tgaggaagga gacacccagg gaggatgagg agcatgagct
     2221 ggaactggac cacctggagc cagagcccca ggctagggac gccacccagg gtgagatgcc
     2281 catgaccacg gaacctgacc ttgacaactg caccgtgcat gtactaacca gccagagccc
     2341 tgtccgccag cgtccagttc gccccaggct ggctgagctg aaagagatgt caccgctaca
     2401 tggcctgggc cacctcatca tggaccagag ctatgtgtgt gccctgattg ccatgatggt
     2461 gtggagcatc atgtaccaca gttggctgac cttcgtcctg ctgctctggg cctgcctcat
     2521 ctggacggtg cgtagccgtc accagctggc tatgctctgc tcgccctgca tcctgctgta
     2581 tgggctgacg ctctgctgcc tgaggtatgt gtgggccatg gaacttcctg agctgcccac
     2641 caccctgggc cctgtcagcc tgcaccagtt gggactggaa cacacacgct acccttgcct
     2701 ggacctcggt gccatgctcc tctatctgct cacattctgg ctccttctgc gtcagttcgt
     2761 gaaggagaag ctgctgaaga agcaaaaggt gcctgcggcg ctgctggagg tcacggtggc
     2821 cgacactgag cccacacaga cccagacgct gctgcggagc ctgggggagc tggtcaccgg
     2881 catctacgtc aaatactgga tctatgtgtg cgccggcatg ttcattgtgg tcagcttcgc
     2941 cggccgcctg gtggtctaca aaatcgtcta catgttcctc ttcctgctgt gcctcacctt
     3001 gttccaggtc tactacaccc tgtggaggaa gctgctacgt gtcttctggt ggctcgtggt
     3061 ggcctataca atgctcgtgc tcatcgctgt gtacaccttc cagttccagg acttccccac
     3121 ctattggcgc aacctcacgg gcttcacaga cgagcagttg ggcgacctgg gcctggagca
     3181 gttcagtgtg tcggagctct tttccagtat cctcatccct ggcttcttcc tgctggcctg
     3241 catcctgcag ctgcactact tccacagacc gttcatgcag ctcactgacc tggagcacgt
     3301 gccgccacca ggcacccgcc accctcgatg ggctcacagg caggatgcag tgagcgaggc
     3361 ccctctgctt gagcatcagg aggaagagga agtcttcagg gaagatgggc agagcatgga
     3421 tgggccccac caggccacac aggtccctga gggtacggcc agcaagtggg gcctggtggc
     3481 tgaccggctg ctggacctag cggccagctt ctcggctgtc ctcacccgaa tccaggtgtt
     3541 cgttcggcgc ttgctagaac ttcacgtctt caagctggtg gccctctaca ctgtctgggt
     3601 ggccctgaaa gaagtgtctg tgatgaacct gctgctggtg gtgctatggg ccttcgcctt
     3661 gccctatccg cgcttccggc ccatggcttc ctgcctgtcc accgtgtgga cctgtatcat
     3721 cattgtatgc aagatgctct atcagctcaa gattgtcaac ccgcatgagt actccagcaa
     3781 ctgcactgag cccttcccca acaataccaa cttgcagcct ttggagatca accagtcttt
     3841 gctgtaccgt ggccctgttg accctgccaa ctggtttggg gtgcggaagg gttaccccaa
     3901 cttgggctat atccagaacc acctgcagat ccttctgttg ctggtgtttg aggccgtggt
     3961 gtaccggcgc caagagcact accgccggca gcaccagcag gcccctctgc ccgcccaggc
     4021 tgtgtgcgca gatggcaccc gccagaggtt ggaccaggac ctacttagct gcctcaagta
     4081 tttcatcaac ttcttcttct acaaattcgg gctggagatc tgcttcttga tggccgtgaa
     4141 tgtgattggg cagcgtatga acttcatggt gatcttacac ggttgctggt tggtggccat
     4201 ccttacacgc cggcgccgtg aggccatcgc ccgcctctgg cctaactact gtctgttcct
     4261 cacgctgttc ctgctgtacc agtacctgct gtgtttgggc atgccccctg cactgtgcat
     4321 tgactatcca tggcgctgga gcaaggccat ccccatgaat tccgccctca tcaagtggct
     4381 gtacctacct gacttcttca gagcccccaa ctccaccaac cttatcagtg acttcctcct
     4441 gctgctttgc gcctcccagc agtggcaggt cttctcagcg gagcgaacgg aggagtggca
     4501 acgcatggcg ggcatcaaca ctgaccacct ggagcccctg cgtggggagc ccaaccctat
     4561 acccaacttc atccactgca ggtcctatct ggatatgctg aaggtggccg tcttccgcta
     4621 cctgttctgg ctggtgctcg ttgtggtgtt tgttgcgggg gccacccgca taagcatctt
     4681 cgggctgggg tacctgctag cctgcttcta cctgctgctg tttggcacta ccctgctgca
     4741 gaaggacacg cgagcccagc tcgtgctgtg ggactgcctc atcctctata atgtcactgt
     4801 catcatctct aagaatatgc tgtcgctcct gtcctgtgtc ttcgtggagc aaatgcagag
     4861 caacttctgc tgggtcatcc agctcttcag cctcgtgtgc acagtcaaag gctactatga
     4921 tcccaaagag atgatgacca gggaccggga ctgcctgctg cctgtggagg aggccgggat
     4981 catctgggac agtatctgct tcttcttcct gctcttgcaa cggcgcatct ttctcagcca
     5041 ctacttcctg catgtcagcg ctgacctgaa agccacagcc ctgcaggcat ccaggggctt
     5101 tgccctctac aatgcagcca acctgaagag catcaacttc catcgccaga ttgaggagaa
     5161 gtccctggcc cagctgaaaa gacagatgaa gcgcatccgt gccaaacagg agaagtacag
     5221 gcagagccag gcaagtcgtg gccaactcca gtccaaagac cctcaggatc ccagccagga
     5281 gccagggcct gacagcccag ggggctcctc cccgccacgg agacagtggt ggcgcccctg
     5341 gctggaccac gccacagtca tccactctgg cgactacttc ctgtttgagt cagatagcga
     5401 ggaggaagag gaggccctac ctgaggaccc caggcctgca gctcagagtg ccttccagat
     5461 ggcataccag gcatgggtaa ccaatgccca gacagtgctg aggcagcgtc gggagcgggc
     5521 acggcaggag cgggcagagc agctggcttc tggaggtgac ttgaacccag atgtggaacc
     5581 agtagatgtc ccagaagatg agatggcagg ccgtagccac atgatgcagc gtgtgctaag
     5641 caccatgcag ttcctgtggg tgctgggcca ggccacggta gacgggctga cgcgctggct
     5701 gcgtgcattc acgaagcacc accgcaccat gagcgatgtg ctgtgcgcag agcgctacct
     5761 gctcacccag gagcttcttc gggttggaga ggtacgccga ggtgtgctgg accagcttta
     5821 tgtgggtgaa gatgaggcca cattgtcagg tcccgtggag acccgggatg gacccagcac
     5881 agcctcaagt gggctgggag ccgaagagcc tttgagtagc atgacagacg acaccagcag
     5941 ccccctgagc acaggctata acacccgcag tggcagtgag gagattgtca ccgacgctgg
     6001 ggacctccag gctgggacct ccctgcacgg ctcccaagag cttttagcca atgctcgtac
     6061 ccggatgcgc acggccagcg agctgctact ggataggcgc ctgcatatcc ctgagctgga
     6121 ggaggccgag cggtttgagg cacagcaggg ccggactctg cggctgctca gggctgggta
     6181 ccagtgcgtg gcggcacact cggagctgct ctgttacttc atcatcatcc ttaaccacat
     6241 ggtgacagcc tcggctgcct ccctggtgct gcccgtgctt gtgttcctgt gggccatgct
     6301 gaccatcccg aggcctagca agcgcttttg gatgacagct atcgtcttca ctgaggtcat
     6361 ggtggtcacc aaatacctgt tccagttcgg cttcttcccc tggaacagct acgttgtgct
     6421 gcggcgctat gagaacaagc cctacttccc tccgcgaatc ctgggccttg agaaaacgga
     6481 cagctacatc aagtatgacc tggtgcagct catggccctc ttcttccacc gctcgcagct
     6541 actgtgttat ggcctctggg accatgagga ggatcgctat cccaaggacc attgcaggag
     6601 tagtgtgaag gaccgggagg ccaaggaaga gccagaagct aagctggaat cgcagtctga
     6661 gacaggcact gggcatccca aggagccagt gttggccggt actcccaggg accacatcca
     6721 agggaaagga agtattagat ccaaggatgt tatccaagat cccccagagg accttaagcc
     6781 ccggcacacg aggcacatca gcatacgctt caggaggcgc aaggagactc caggacccaa
     6841 aggaacagca gtcatggaga ctgagcacga ggagggagaa ggaaaagaaa ctacagagag
     6901 aaagaggccg cgtcacactc aagaaaaatc gaagtttcgg gagagaatga aggcagctgg
     6961 gcgccggctg cagagcttct gtgtgtcact ggcccagagc ttctaccaac ccttgcagcg
     7021 tttcttccat gacattctgc acacaaagta ccgggcggcc accgacgtct acgccctcat
     7081 gttcctggcc gatattgtcg acatcatcat catcatcttt ggtttttggg cttttgggaa
     7141 gcactctgca gccacagaca ttgcatcctc gctgtcagat gaccaggtgc cacaggcctt
     7201 cctgttcatg ctgctggtcc agtttggcac catggtcatc gaccgtgccc tctacctgcg
     7261 caagactgtc ctgggaaagc tggcctttca ggtggtcctg gtggtggcga ttcacatctg
     7321 gatgttcttt atcttaccgg ctgtcactga gaggatgttc agccagaatg cggtggcaca
     7381 gctgtggtac ttcgtcaagt gcatttactt tgccctgtcc gcctaccaga tccgctgtgg
     7441 ctaccccacc cgtatcttgg gcaacttcct caccaagaaa tacaaccatc taaacctctt
     7501 ccttttccag gggttccgtc tagtgccgtt cctggtggag ctgcgggccg tcatggactg
     7561 ggtgtggacc gacaccacgc tgtccctgtc caactggatg tgtgtggaag acatctatgc
     7621 caacatcttt atcatcaagt gcagccgaga gacagagaag aaataccccc agcccaaggg
     7681 gcagaagaag aagaaaattg tcaagtatgg tatgggaggc ctcattatcc tcttcctcat
     7741 cgccatcatc tggttccctc tgctcttcat gtcactgatc cgctctgtgg tcggggtcgt
     7801 caaccagccc attgatgtca ctgtcaccct caagctaggc ggctatgagc cactgttcac
     7861 catgagcgcc cagcagccat ccattgtgcc attcacaccc caggcctacg aggagctgtc
     7921 ccagcagttt gacccctatc cactagccat gcagttcatt agccagtaca gtcctgagga
     7981 catcgtcact gcacagatcg agggcagctc gggggcgctg tggcgcatca gcccacccag
     8041 ccgagcccag atgaagcagg agctgtacaa cggcacagcc gacattacac tgcgctttac
     8101 ctggaatttc caaagggacc tggccaaggg tggcactgtg gagtatacta atgagaagca
     8161 caccttggag ctggccccca acagtacggc acgaaggcag ctggcccaac tgctcgaggg
     8221 cagacctgac cagtcagtgg tcattcccca tctcttcccc aagtacattc gtgctcccaa
     8281 tgggcctgaa gccaaccctg tgaagcagct gcagccagat gaggaagagg actaccttgg
     8341 tgtgcgcatc cagctgcgga gggagcaagt gggcacaggg gcctctgggg agcaagcggg
     8401 caccaaggcc tccgacttcc tcgagtggtg ggtcatcgag ctgcaggact gcaaggctga
     8461 ctgcaacctg ctgcccatgg tcatcttcag tgacaaggtc agcccaccta gcctgggctt
     8521 cctggccggc tacgggattg tggggctgta tgtctccatc gtgctggtgg ttggcaagtt
     8581 tgtgcggggc ttcttcagcg agatctctca ctccatcatg ttcgaggaac tgccgtgtgt
     8641 ggaccgcatc ctcaagctgt gccaggacat cttcttggtg cgcgagaccc gggagctgga
     8701 gctggaggag gagctatacg ccaagctcat cttcctgtac cgatctccag agaccatgat
     8761 taagtggaca cgtgagaggg agtaagaatt cagatccgcc cctctccctc ccccccccct
     8821 aacgttactg gccgaagccg cttggaataa ggccggtgtg cgtttgtcta tatgttattt
     8881 tccaccatat tgccgtcttt tggcaatgtg agggcccgga aacctggccc tgtcttcttg
     8941 acgagcattc ctaggggtct ttcccctctc gccaaaggaa tgcaaggtct gttgaatgtc
     9001 gtgaaggaag cagttcctct ggtagcttct tgaagacaaa caacgtctgt agcgaccctt
     9061 tgcaggcagc ggaacccccc acctggcgac aggtgcctct gcggccaaaa gccacgtgta
     9121 taagatacac ctgcaaaggc ggcacaaccc cagtgccacg ttgtgagttg gatagttgtg
     9181 gaaagagtca aatggctctc ctcaagcgta ttcaacaagg ggctgaagga tgcccagaag
     9241 gtaccccatt gtatgggatc tgatctgggg cctcggtaca catgctttac atgtgtttag
     9301 tcgaggttaa aaaaacgtct aggccccccg aaccacgggg acgtggtttt cctttgaaaa
     9361 acacgatgat aatatggcca caaccatggt gagcaagggc gaggaggata acatggccat
     9421 catcaaggag ttcatgcgct tcaaggtgca catggagggc tccgtgaacg gccacgagtt
     9481 cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg ccaagctgaa
     9541 ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc agttcatgta
     9601 cggctccaag gcctacgtga agcaccccgc cgacatcccc gactacttga agctgtcctt
     9661 ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt
     9721 gacccaggac tcctccctgc aggacggcga gttcatctac aaggtgaagc tgcgcggcac
     9781 caacttcccc tccgacggcc ccgtaatgca gaagaagacc atgggctggg aggcctcctc
     9841 cgagcggatg taccccgagg acggcgccct gaagggcgag atcaagcaga ggctgaagct
     9901 gaaggacggc ggccactacg acgctgaggt caagaccacc tacaaggcca agaagcccgt
     9961 gcagctgccc ggcgcctaca acgtcaacat caagttggac atcacctccc acaacgagga
    10021 ctacaccatc gtggaacagt acgaacgcgc cgagggccgc cactccaccg gcggcatgga
    10081 cgagctgtac aagtaaaccg gtgctcgagg taccgatgaa tagctaaggt cgaggccgca
    10141 ggtaagtatc aaggttacaa gacaggttta aggagaccaa tagaaactgg gcttgtcgag
    10201 acagagaaga ctcttgcgtt tctgataggc acctattggt cttactgaca tccactttgc
    10261 ctttctctcc acaggtgtcg acaatcaacc tctggattac aaaatttgtg aaagattgac
    10321 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt
    10381 gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt
    10441 gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt
    10501 gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg
    10561 gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg
    10621 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaagct
    10681 gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt
    10741 ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc
    10801 tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc
    10861 cgcctccccg cctggaattc gagctcggta cgatcagcct cgactgtgcc ttctagttgc
    10921 cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc
    10981 actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct
    11041 attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga caatagccca
    11101 gcttttgttc cctttagtga gggttaattg cgcgcttggc gtaatcatgg tcatagctgt
    11161 ttcctgtgtg aaattgttat ccgctaattc actcctcagg tgcaggctgc ctatcagaag
    11221 gtggtggctg gtgtggccaa tgccctggct cacaaatacc actgagatct ttttccctct
    11281 gccaaaaatt atggggacat catgaagccc cttgagcatc tgacttctgg ctaataaagg
    11341 aaatttattt tcattgcaat agtgtgttgg aattttttgt gtctctcact cggaaggaca
    11401 tatgggaggg caaatcattt aaaacatcag aatgagtatt tggtttagag tttggcaaca
    11461 tatgcccata tgctggctgc catgaacaaa ggttggctat aaagaggtca tcagtatatg
    11521 aaacagcccc ctgctgtcca ttccttattc catagaaaag ccttgacttg aggttagatt
    11581 ttttttatat tttgttttgt gttatttttt tctttaacat ccctaaaatt ttccttacat
    11641 gttttactag ccagattttt cctcctctcc tgactactcc cagtcatagc tgtccctctt
    11701 ctcttatgga gatcggccgg ccgccggcgt cgacgcgcgc ggccgcggcg cgccgttgct
    11761 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
    11821 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
    11881 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
    11941 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
    12001 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
    12061 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
    12121 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
    12181 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
    12241 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
    12301 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
    12361 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
    12421 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
    12481 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
    12541 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
    12601 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
    12661 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
    12721 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
    12781 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
    12841 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
    12901 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
    12961 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
    13021 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
    13081 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
    13141 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
    13201 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
    13261 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
    13321 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
    13381 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
    13441 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
    13501 ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa
    13561 taggcgtatc acgaggccct ttcgtctacg cgcgcgccgg cggcgcgccg tcgacggccg
    13621 gccgcggccg c
//
