LOCUS       sequence-270572-        8674 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of VN-fused and myc-tagged HLA-A*01:01.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 8674)
  AUTHORS   McShan AC, Devlin CA, Overall SA, Park J, Toor JS, Moschidi D, 
            Flores-Solis D, Choi H, Tripathi S, Procko E, Sgourakis NG
  TITLE     Molecular determinants of chaperone interactions on MHC-I for 
            folding and antigen repertoire selection.
  JOURNAL   Proc Natl Acad Sci U S A. 2019 Dec 17;116(51):25602-25613. doi: 
            10.1073/pnas.1915562116. Epub 2019 Dec 3.
  PUBMED    31796585
REFERENCE   2  (bases 1 to 8674)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1915562116"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2019-12-17- 17"; volume: "116"; issue: "51"; pages: 
            "25602-25613"
COMMENT     Sequence Label: pCEP4-myc-HLA-A1-VN
FEATURES             Location/Qualifiers
     source          1..8674
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(252..270)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        338..442
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(443..511,512..1303)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(661..680)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1474..2062
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1963..1982
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        2493..2638
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             2681..3718
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(3716..3735)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    3760..3808
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        4462..4841
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4842..5045
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4996..5016
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5042..5066
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             5148..5177
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             join(6225..6227,6228..6686)
                     /codon_start=1
                     /product="improved N-terminal fragment of mVenus for use in
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN155(I152L)
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYLTA"
     primer_bind     complement(6267..6288)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6528..6547)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     polyA_signal    6709..6843
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6733..6752)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6787..6806
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(6986..7008)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 ctcgcagccc ggaaaactcc cgctgaacat cctcaagatt tgcgtcctga gcctcaagcc
       61 aggcctcaaa ttcctcgtcc ccctttttgc tggacggtag ggatggggat tctcgggacc
      121 cctcctcttc ctcttcaagg tcaccagaca gagatgctac tggggcaacg gaagaaaagc
      181 tgggtgcggc ctgtgaggat cagcttatcg atgataagct gtcaaacatg agaattcttg
      241 aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt
      301 ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
      361 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
      421 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
      481 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
      541 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
      601 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
      661 gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat
      721 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
      781 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
      841 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
      901 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
      961 cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac
     1021 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     1081 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     1141 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
     1201 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     1261 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     1321 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     1381 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     1441 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     1501 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     1561 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     1621 ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     1681 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     1741 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     1801 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     1861 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     1921 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     1981 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     2041 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     2101 ttgctggcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg
     2161 caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca
     2221 gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccccga gatgcgccgc
     2281 gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca
     2341 ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg
     2401 aggtgccgcc ctgcttcatc cccgtggccc gttgctcgcg tttgctggcg gtgtccccgg
     2461 aagaaatata tttgcatgtc tttagttcta tgatgacaca aaccccgccc agcgtcttgt
     2521 cattggcgaa ttcgaacacg cagatgcagt cggggcggcg cggtccgagg tccacttcgc
     2581 atattaaggt gacgcgtgtg gcctcgaaca ccgagcgacc ctgcagcgac ccgcttaaca
     2641 gcgtcaacag cgtgccgcag atcccggggg gcaatgagat atgaaaaagc ctgaactcac
     2701 cgcgacgtct gtcgagaagt ttctgatcga aaagttcgac agcgtctccg acctgatgca
     2761 gctctcggag ggcgaagaat ctcgtgcttt cagcttcgat gtaggagggc gtggatatgt
     2821 cctgcgggta aatagctgcg ccgatggttt ctacaaagat cgttatgttt atcggcactt
     2881 tgcatcggcc gcgctcccga ttccggaagt gcttgacatt ggggaattca gcgagagcct
     2941 gacctattgc atctcccgcc gtgcacaggg tgtcacgttg caagacctgc ctgaaaccga
     3001 actgcccgct gttctgcagc cggtcgcgga ggccatggat gcgatcgctg cggccgatct
     3061 tagccagacg agcgggttcg gcccattcgg accgcaagga atcggtcaat acactacatg
     3121 gcgtgatttc atatgcgcga ttgctgatcc ccatgtgtat cactggcaaa ctgtgatgga
     3181 cgacaccgtc agtgcgtccg tcgcgcaggc tctcgatgag ctgatgcttt gggccgagga
     3241 ctgccccgaa gtccggcacc tcgtgcacgc ggatttcggc tccaacaatg tcctgacgga
     3301 caatggccgc ataacagcgg tcattgactg gagcgaggcg atgttcgggg attcccaata
     3361 cgaggtcgcc aacatcttct tctggaggcc gtggttggct tgtatggagc agcagacgcg
     3421 ctacttcgag cggaggcatc cggagcttgc aggatcgccg cggctccggg cgtatatgct
     3481 ccgcattggt cttgaccaac tctatcagag cttggttgac ggcaatttcg atgatgcagc
     3541 ttgggcgcag ggtcgatgcg acgcaatcgt ccgatccgga gccgggactg tcgggcgtac
     3601 acaaatcgcc cgcagaagcg cggccgtctg gaccgatggc tgtgtagaag tactcgccga
     3661 tagtggaaac cgacgcccca gcactcgtcc ggatcgggag atgggggagg ctaactgaaa
     3721 cacggaagga gacaataccg gaaggaaccc gcgctatgac ggcaataaaa agacagaata
     3781 aaacgcacgg gtgttgggtc gtttgttcat aaacgcgggg ttcggtccca gggctggcac
     3841 tctgtcgata ccccaccgag accccattgg ggccaatacg cccgcgtttc ttccttttcc
     3901 ccaccccacc ccccaagttc gggtgaaggc ccagggctcg cagccaacgt cggggcggca
     3961 ggccctgcca tagccactgg ccccgtgggt tagggacggg gtcccccatg gggaatggtt
     4021 tatggttcgt gggggttatt attttgggcg ttgcgtgggg tcaggtccac gactggactg
     4081 agcagacaga cccatggttt ttggatggcc tgggcatgga ccgcatgtac tggcgcgaca
     4141 cgaacaccgg gcgtctgtgg ctgccaaaca cccccgaccc ccaaaaacca ccgcgcggat
     4201 ttctggcgtg ccaagctagt cgaccaattc tcatgtttga cagcttatca tcgcagatcc
     4261 gggcaacgtt gttgccattg ctgcaggcgc agaactggta ggtatggaag atctatacat
     4321 tgaatcaata ttggcaatta gccatattag tcattggtta tatagcataa atcaatattg
     4381 gctattggcc attgcatacg ttgtatctat atcataatat gtacatttat attggctcat
     4441 gtccaatatg accgccatgt tgacattgat tattgactag ttattaatag taatcaatta
     4501 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
     4561 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
     4621 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
     4681 ctgcccactt ggcagtacat caagtgtatc atatgccaag tccgccccct attgacgtca
     4741 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttacgg gactttccta
     4801 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
     4861 acaccaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
     4921 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaata
     4981 accccgcccc gttgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
     5041 gagctcgttt agtgaaccgt cagatctcta gaagctgggt accagctgct agcgccacca
     5101 tgaagaccat catcgccctg agctacatct tctgcctggt gttcgccgag cagaaattga
     5161 tcagcgagga ggacctgggc agccctggag gcagttcagg aggcggatct cattctatga
     5221 gatatttctt cacatccgtg tcccggcccg gccgcgggga gccccgcttc atcgccgtgg
     5281 gctacgtgga cgacacgcag ttcgtgcggt tcgacagcga cgccgcgagc cagaagatgg
     5341 agccgcgggc gccgtggata gagcaggagg ggccggagta ttgggaccag gagacacgga
     5401 atatgaaggc ccactcacag actgaccgag cgaacctggg gaccctgcgc ggctactaca
     5461 accagagcga ggacggttct cacaccatcc agataatgta tggctgcgac gtggggccgg
     5521 acgggcgctt cctccgcggg taccggcagg acgcctacga cggcaaggat tacatcgccc
     5581 tgaacgagga cctgcgttct tggaccgcgg cggacatggc agctcagatc accaagcgca
     5641 agtgggaggc ggtccatgcg gcggagcagc ggagagtcta cctggagggc cggtgcgtgg
     5701 acgggctccg cagatacctg gagaacggga aggagacgct gcagcgcacg gaccccccca
     5761 agacacatat gacccaccac cccatctctg accatgaggc caccctgagg tgctgggccc
     5821 tgggcttcta ccctgcggag atcacactga cctggcagcg ggatggggag gaccagaccc
     5881 aggacacgga gctcgtggag accaggcctg caggggatgg aaccttccag aagtgggcgg
     5941 ctgtggtggt gccttctgga gaggagcaga gatacacctg ccatgtgcag catgagggtc
     6001 tgcccaagcc cctcaccctg agatgggagc tgtcttccca gcccactata ccgattgttg
     6061 gcatcatagc cggacttgta ttgctgggtg cggtgattac gggcgccgtc gtcgctgctg
     6121 tgatgtggcg gcggaagtct tcagacagaa aaggcggatc ttatacccaa gctgcaagct
     6181 cagattcagc acaggggtct gatgtgagtc tgacagcttg caaagtgagc aagggcgagg
     6241 agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca
     6301 agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagc
     6361 tgatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgggct
     6421 acggcctgca gtgcttcgcc cgctaccccg accacatgaa gcagcacgac ttcttcaagt
     6481 ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact
     6541 acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga
     6601 agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca
     6661 acagccacaa cgtctatctg accgcctaac tcgaggccgg caaggccgga tccagacatg
     6721 ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt
     6781 atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa
     6841 gttaacaaca acaattgcat tcattttatg tttcaggttc agggggaggt gtgggaggtt
     6901 ttttaaagca agtaaaacct ctacaaatgt ggtatggctg attatgatcc ggctgcctcg
     6961 cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag
     7021 cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg
     7081 gcgggtgtcg gggcgcagcc atgaggtcga ctctagagga tcgatccccg ccccggacga
     7141 actaaacctg actacgacat ctctgcccct tcttcgcggg gcagtgcatg taatcccttc
     7201 agttggttgg tacaacttgc caactgggcc ctgttccaca tgtgacacgg ggggggacca
     7261 aacacaaagg ggttctctga ctgtagttga catccttata aatggatgtg cacatttgcc
     7321 aacactgagt ggctttcatc ctggagcaga ctttgcagtc tgtggactgc aacacaacat
     7381 tgcctttatg tgtaactctt ggctgaagct cttacaccaa tgctggggga catgtacctc
     7441 ccaggggccc aggaagacta cgggaggcta caccaacgtc aatcagaggg gcctgtgtag
     7501 ctaccgataa gcggaccctc aagagggcat tagcaatagt gtttataagg cccccttgtt
     7561 aaccctaaac gggtagcata tgcttcccgg gtagtagtat atactatcca gactaaccct
     7621 aattcaatag catatgttac ccaacgggaa gcatatgcta tcgaattagg gttagtaaaa
     7681 gggtcctaag gaacagcgat atctcccacc ccatgagctg tcacggtttt atttacatgg
     7741 ggtcaggatt ccacgagggt agtgaaccat tttagtcaca agggcagtgg ctgaagatca
     7801 aggagcgggc agtgaactct cctgaatctt cgcctgcttc ttcattctcc ttcgtttagc
     7861 taatagaata actgctgagt tgtgaacagt aaggtgtatg tgaggtgctc gaaaacaagg
     7921 tttcaggtga cgcccccaga ataaaatttg gacggggggt tcagtggtgg cattgtgcta
     7981 tgacaccaat ataaccctca caaacccctt gggcaataaa tactagtgta ggaatgaaac
     8041 attctgaata tctttaacaa tagaaatcca tggggtgggg acaagccgta aagactggat
     8101 gtccatctca cacgaattta tggctatggg caacacataa tcctagtgca atatgatact
     8161 ggggttatta agatgtgtcc caggcaggga ccaagacagg tgaaccatgt tgttacactc
     8221 tatttgtaac aaggggaaag agagtggacg ccgacagcag cggactccac tggttgtctc
     8281 taacaccccc gaaaattaaa cggggctcca cgccaatggg gcccataaac aaagacaagt
     8341 ggccactctt ttttttgaaa ttgtggagtg ggggcacgcg tcagccccca cacgccgccc
     8401 tgcggttttg gactgtaaaa taagggtgta ataacttggc tgattgtaac cccgctaacc
     8461 actgcggtca aaccacttgc ccacaaaacc actaatggca ccccggggaa tacctgcata
     8521 agtaggtggg cgggccaaga taggggcgcg attgctgcga tctggaggac aaattacaca
     8581 cacttgcgcc tgagcgccaa gcacagggtt gttggtcctc atattcacga ggtcgctgag
     8641 agcacggtgg gctaatgttg ccatgggtag cata
//
