LOCUS       sequence-270573-       10531 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of VN-fused and myc-tagged H2-Dd 
            (Y84C/C121S/A139C mutant).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 10531)
  AUTHORS   McShan AC, Devlin CA, Overall SA, Park J, Toor JS, Moschidi D, 
            Flores-Solis D, Choi H, Tripathi S, Procko E, Sgourakis NG
  TITLE     Molecular determinants of chaperone interactions on MHC-I for 
            folding and antigen repertoire selection.
  JOURNAL   Proc Natl Acad Sci U S A. 2019 Dec 17;116(51):25602-25613. doi: 
            10.1073/pnas.1915562116. Epub 2019 Dec 3.
  PUBMED    31796585
REFERENCE   2  (bases 1 to 10531)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1915562116"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2019-12-17- 17"; volume: "116"; issue: "51"; pages: 
            "25602-25613"
COMMENT     Sequence Label: pCEP4-myc-H2-Dd-VN (Y84C/C121S/A139C)
FEATURES             Location/Qualifiers
     source          1..10531
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(170..188)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        256..360
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(361..429,430..1221)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(579..598)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1392..1980
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1881..1900
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        2411..2556
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             2599..3636
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(3634..3653)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    3678..3726
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        4380..4759
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4760..4963
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4914..4934
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     4960..4984
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             5066..5095
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             join(6143..6145,6146..6604)
                     /codon_start=1
                     /product="improved N-terminal fragment of mVenus for use in
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN155(I152L)
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYLTA"
     primer_bind     complement(6185..6206)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6446..6465)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     polyA_signal    6627..6761
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6651..6670)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6705..6724
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(6904..6926)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      7486..9275
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
ORIGIN
        1 ctttttgctg gacggtaggg atggggattc tcgggacccc tcctcttcct cttcaaggtc
       61 accagacaga gatgctactg gggcaacgga agaaaagctg ggtgcggcct gtgaggatca
      121 gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata
      181 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact
      241 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
      301 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
      361 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
      421 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
      481 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
      541 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
      601 cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
      661 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
      721 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
      781 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
      841 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
      901 cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
      961 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     1021 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     1081 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     1141 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     1201 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     1261 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     1321 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     1381 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     1441 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     1501 gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta
     1561 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     1621 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     1681 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     1741 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     1801 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     1861 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     1921 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     1981 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttg aagctgtccc
     2041 tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg
     2101 ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc
     2161 aagacgtagc ccagcgcgtc ggccccgaga tgcgccgcgt gcggctgctg gagatggcgg
     2221 acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt
     2281 gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccct gcttcatccc
     2341 cgtggcccgt tgctcgcgtt tgctggcggt gtccccggaa gaaatatatt tgcatgtctt
     2401 tagttctatg atgacacaaa ccccgcccag cgtcttgtca ttggcgaatt cgaacacgca
     2461 gatgcagtcg gggcggcgcg gtccgaggtc cacttcgcat attaaggtga cgcgtgtggc
     2521 ctcgaacacc gagcgaccct gcagcgaccc gcttaacagc gtcaacagcg tgccgcagat
     2581 cccggggggc aatgagatat gaaaaagcct gaactcaccg cgacgtctgt cgagaagttt
     2641 ctgatcgaaa agttcgacag cgtctccgac ctgatgcagc tctcggaggg cgaagaatct
     2701 cgtgctttca gcttcgatgt aggagggcgt ggatatgtcc tgcgggtaaa tagctgcgcc
     2761 gatggtttct acaaagatcg ttatgtttat cggcactttg catcggccgc gctcccgatt
     2821 ccggaagtgc ttgacattgg ggaattcagc gagagcctga cctattgcat ctcccgccgt
     2881 gcacagggtg tcacgttgca agacctgcct gaaaccgaac tgcccgctgt tctgcagccg
     2941 gtcgcggagg ccatggatgc gatcgctgcg gccgatctta gccagacgag cgggttcggc
     3001 ccattcggac cgcaaggaat cggtcaatac actacatggc gtgatttcat atgcgcgatt
     3061 gctgatcccc atgtgtatca ctggcaaact gtgatggacg acaccgtcag tgcgtccgtc
     3121 gcgcaggctc tcgatgagct gatgctttgg gccgaggact gccccgaagt ccggcacctc
     3181 gtgcacgcgg atttcggctc caacaatgtc ctgacggaca atggccgcat aacagcggtc
     3241 attgactgga gcgaggcgat gttcggggat tcccaatacg aggtcgccaa catcttcttc
     3301 tggaggccgt ggttggcttg tatggagcag cagacgcgct acttcgagcg gaggcatccg
     3361 gagcttgcag gatcgccgcg gctccgggcg tatatgctcc gcattggtct tgaccaactc
     3421 tatcagagct tggttgacgg caatttcgat gatgcagctt gggcgcaggg tcgatgcgac
     3481 gcaatcgtcc gatccggagc cgggactgtc gggcgtacac aaatcgcccg cagaagcgcg
     3541 gccgtctgga ccgatggctg tgtagaagta ctcgccgata gtggaaaccg acgccccagc
     3601 actcgtccgg atcgggagat gggggaggct aactgaaaca cggaaggaga caataccgga
     3661 aggaacccgc gctatgacgg caataaaaag acagaataaa acgcacgggt gttgggtcgt
     3721 ttgttcataa acgcggggtt cggtcccagg gctggcactc tgtcgatacc ccaccgagac
     3781 cccattgggg ccaatacgcc cgcgtttctt ccttttcccc accccacccc ccaagttcgg
     3841 gtgaaggccc agggctcgca gccaacgtcg gggcggcagg ccctgccata gccactggcc
     3901 ccgtgggtta gggacggggt cccccatggg gaatggttta tggttcgtgg gggttattat
     3961 tttgggcgtt gcgtggggtc aggtccacga ctggactgag cagacagacc catggttttt
     4021 ggatggcctg ggcatggacc gcatgtactg gcgcgacacg aacaccgggc gtctgtggct
     4081 gccaaacacc cccgaccccc aaaaaccacc gcgcggattt ctggcgtgcc aagctagtcg
     4141 accaattctc atgtttgaca gcttatcatc gcagatccgg gcaacgttgt tgccattgct
     4201 gcaggcgcag aactggtagg tatggaagat ctatacattg aatcaatatt ggcaattagc
     4261 catattagtc attggttata tagcataaat caatattggc tattggccat tgcatacgtt
     4321 gtatctatat cataatatgt acatttatat tggctcatgt ccaatatgac cgccatgttg
     4381 acattgatta ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc
     4441 atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
     4501 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
     4561 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
     4621 agtgtatcat atgccaagtc cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
     4681 gcattatgcc cagtacatga ccttacggga ctttcctact tggcagtaca tctacgtatt
     4741 agtcatcgct attaccatgg tgatgcggtt ttggcagtac accaatgggc gtggatagcg
     4801 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg
     4861 gcaccaaaat caacgggact ttccaaaatg tcgtaataac cccgccccgt tgacgcaaat
     4921 gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaaccgtca
     4981 gatctctaga agctgggtac cagctgctag cgccaccatg aagaccatca tcgccctgag
     5041 ctacatcttc tgcctggtgt tcgccgagca gaaattgatc agcgaggagg acctgggcag
     5101 ccctggaggc agttcaggag gcggatcaca ctcgctgagg tatttcgtca ccgccgtgtc
     5161 ccggcccggc ttcggggagc cccggtacat ggaagtcggc tacgtggaca acacggagtt
     5221 cgtgcgcttc gacagcgacg cggagaatcc gagatatgag ccgcgggcgc ggtggataga
     5281 gcaggagggg ccggagtatt gggagcggga gacacgaaga gccaagggca atgagcagag
     5341 tttccgagtg gacctgagga ccgcgctgcg ctgctacaac cagagcgcgg gcggctctca
     5401 cacactccag tggatggctg gctgtgacgt ggagtcggac gggcgcctcc tccgcgggta
     5461 ctggcagttc gcctacgacg gcagcgatta catcgccctg aacgaagacc tgaaaacgtg
     5521 gacggcggcg gacatgtgcg cgcagatcac ccgacgcaag tgggagcagg ctggtgctgc
     5581 agagagagac cgggcctacc tggagggcga gtgcgtggag tggctccgca gatacctgaa
     5641 gaacgggaat gctacgctgc tgcgcacaga tcccccaaag gcccatgtga cccatcaccg
     5701 cagacctgaa ggtgatgtca ccctgaggtg ctgggccctg ggcttctacc ctgctgacat
     5761 caccctgacc tggcagttga atggggagga gctgacccag gaaatggagc ttgtggagac
     5821 caggcctgca ggggatggaa ccttccagaa gtgggcatct gtggtggtgc ctcttgggaa
     5881 ggagcagaag tacacatgcc atgtggaaca tgaggggctg cctgagcccc tcaccctgag
     5941 atggggcaag gaggaacccc catcctcaac aaagactaat actgttatta tcgcagtacc
     6001 agtagttttg ggtgcagtag taattttggg agccgtaatg gcatttgtta tgaagaggcg
     6061 gcgcaacact ggtgggaagg gcggagacta tgctttggca cccggcagcc agtcctctga
     6121 catgtccttg cctgactgta aggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc
     6181 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg
     6241 cgagggcgat gccacctacg gcaagctgac cctgaagctg atctgcacca ccggcaagct
     6301 gcccgtgccc tggcccaccc tcgtgaccac cctgggctac ggcctgcagt gcttcgcccg
     6361 ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt
     6421 ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa
     6481 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga
     6541 cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg tctatctgac
     6601 cgcctaactc gaggccggca aggccggatc cagacatgat aagatacatt gatgagtttg
     6661 gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta
     6721 ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc
     6781 attttatgtt tcaggttcag ggggaggtgt gggaggtttt ttaaagcaag taaaacctct
     6841 acaaatgtgg tatggctgat tatgatccgg ctgcctcgcg cgtttcggtg atgacggtga
     6901 aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg
     6961 gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat
     7021 gaggtcgact ctagaggatc gatccccgcc ccggacgaac taaacctgac tacgacatct
     7081 ctgccccttc ttcgcggggc agtgcatgta atcccttcag ttggttggta caacttgcca
     7141 actgggccct gttccacatg tgacacgggg ggggaccaaa cacaaagggg ttctctgact
     7201 gtagttgaca tccttataaa tggatgtgca catttgccaa cactgagtgg ctttcatcct
     7261 ggagcagact ttgcagtctg tggactgcaa cacaacattg cctttatgtg taactcttgg
     7321 ctgaagctct tacaccaatg ctgggggaca tgtacctccc aggggcccag gaagactacg
     7381 ggaggctaca ccaacgtcaa tcagaggggc ctgtgtagct accgataagc ggaccctcaa
     7441 gagggcatta gcaatagtgt ttataaggcc cccttgttaa ccctaaacgg gtagcatatg
     7501 cttcccgggt agtagtatat actatccaga ctaaccctaa ttcaatagca tatgttaccc
     7561 aacgggaagc atatgctatc gaattagggt tagtaaaagg gtcctaagga acagcgatat
     7621 ctcccacccc atgagctgtc acggttttat ttacatgggg tcaggattcc acgagggtag
     7681 tgaaccattt tagtcacaag ggcagtggct gaagatcaag gagcgggcag tgaactctcc
     7741 tgaatcttcg cctgcttctt cattctcctt cgtttagcta atagaataac tgctgagttg
     7801 tgaacagtaa ggtgtatgtg aggtgctcga aaacaaggtt tcaggtgacg cccccagaat
     7861 aaaatttgga cggggggttc agtggtggca ttgtgctatg acaccaatat aaccctcaca
     7921 aaccccttgg gcaataaata ctagtgtagg aatgaaacat tctgaatatc tttaacaata
     7981 gaaatccatg gggtggggac aagccgtaaa gactggatgt ccatctcaca cgaatttatg
     8041 gctatgggca acacataatc ctagtgcaat atgatactgg ggttattaag atgtgtccca
     8101 ggcagggacc aagacaggtg aaccatgttg ttacactcta tttgtaacaa ggggaaagag
     8161 agtggacgcc gacagcagcg gactccactg gttgtctcta acacccccga aaattaaacg
     8221 gggctccacg ccaatggggc ccataaacaa agacaagtgg ccactctttt ttttgaaatt
     8281 gtggagtggg ggcacgcgtc agcccccaca cgccgccctg cggttttgga ctgtaaaata
     8341 agggtgtaat aacttggctg attgtaaccc cgctaaccac tgcggtcaaa ccacttgccc
     8401 acaaaaccac taatggcacc ccggggaata cctgcataag taggtgggcg ggccaagata
     8461 ggggcgcgat tgctgcgatc tggaggacaa attacacaca cttgcgcctg agcgccaagc
     8521 acagggttgt tggtcctcat attcacgagg tcgctgagag cacggtgggc taatgttgcc
     8581 atgggtagca tatactaccc aaatatctgg atagcatatg ctatcctaat ctatatctgg
     8641 gtagcatagg ctatcctaat ctatatctgg gtagcatatg ctatcctaat ctatatctgg
     8701 gtagtatatg ctatcctaat ttatatctgg gtagcatagg ctatcctaat ctatatctgg
     8761 gtagcatatg ctatcctaat ctatatctgg gtagtatatg ctatcctaat ctgtatccgg
     8821 gtagcatatg ctatcctaat agagattagg gtagtatatg ctatcctaat ttatatctgg
     8881 gtagcatata ctacccaaat atctggatag catatgctat cctaatctat atctgggtag
     8941 catatgctat cctaatctat atctgggtag cataggctat cctaatctat atctgggtag
     9001 catatgctat cctaatctat atctgggtag tatatgctat cctaatttat atctgggtag
     9061 cataggctat cctaatctat atctgggtag catatgctat cctaatctat atctgggtag
     9121 tatatgctat cctaatctgt atccgggtag catatgctat cctcatgcat atacagtcag
     9181 catatgatac ccagtagtag agtgggagtg ctatcctttg catatgccgc cacctcccaa
     9241 gggggcgtga attttcgctg cttgtccttt tcctgctggt tgctcccatt cttaggtgaa
     9301 tttaaggagg ccaggctaaa gccgtcgcat gtctgattgc tcaccaggta aatgtcgcta
     9361 atgttttcca acgcgagaag gtgttgagcg cggagctgag tgacgtgaca acatgggtat
     9421 gcccaattgc cccatgttgg gaggacgaaa atggtgacaa gacagatggc cagaaataca
     9481 ccaacagcac gcatgatgtc tactggggat ttattcttta gtgcggggga atacacggct
     9541 tttaatacga ttgagggcgt ctcctaacaa gttacatcac tcctgccctt cctcaccctc
     9601 atctccatca cctccttcat ctccgtcatc tccgtcatca ccctccgcgg cagccccttc
     9661 caccataggt ggaaaccagg gaggcaaatc tactccatcg tcaaagctgc acacagtcac
     9721 cctgatattg caggtaggag cgggctttgt cataacaagg tccttaatcg catccttcaa
     9781 aacctcagca aatatatgag tttgtaaaaa gaccatgaaa taacagacaa tggactccct
     9841 tagcgggcca ggttgtgggc cgggtccagg ggccattcca aaggggagac gactcaatgg
     9901 tgtaagacga cattgtggaa tagcaagggc agttcctcgc cttaggttgt aaagggaggt
     9961 cttactacct ccatatacga acacaccggc gacccaagtt ccttcgtcgg tagtcctttc
    10021 tacgtgactc ctagccagga gagctcttaa accttctgca atgttctcaa atttcgggtt
    10081 ggaacctcct tgaccacgat gctttccaaa ccaccctcct tttttgcgcc tgcctccatc
    10141 accctgaccc cggggtccag tgcttgggcc ttctcctggg tcatctgcgg ggccctgctc
    10201 tatcgctccc gggggcacgt caggctcacc atctgggcca ccttcttggt ggtattcaaa
    10261 ataatcggct tcccctacag ggtggaaaaa tggccttcta cctggagggg gcctgcgcgg
    10321 tggagacccg gatgatgatg actgactact gggactcctg ggcctctttt ctccacgtcc
    10381 acgacctctc cccctggctc tttcacgact tccccccctg gctctttcac gtcctctacc
    10441 ccggcggcct ccactacctc ctcgaccccg gcctccacta cctcctcgac cccggcctcc
    10501 actgcctcct cgaccccggc ctccacctcc t
//
