LOCUS       sequence-270574-        9560 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of myc-tagged HLA-A*01:01.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 9560)
  AUTHORS   McShan AC, Devlin CA, Overall SA, Park J, Toor JS, Moschidi D, 
            Flores-Solis D, Choi H, Tripathi S, Procko E, Sgourakis NG
  TITLE     Molecular determinants of chaperone interactions on MHC-I for 
            folding and antigen repertoire selection.
  JOURNAL   Proc Natl Acad Sci U S A. 2019 Dec 17;116(51):25602-25613. doi: 
            10.1073/pnas.1915562116. Epub 2019 Dec 3.
  PUBMED    31796585
REFERENCE   2  (bases 1 to 9560)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1915562116"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2019-12-17- 17"; volume: "116"; issue: "51"; pages: 
            "25602-25613"
COMMENT     Sequence Label: pCEP4-myc-HLA-A1
FEATURES             Location/Qualifiers
     source          1..9560
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(708..726)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        794..898
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(899..967,968..1759)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1117..1136)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1930..2518
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2419..2438
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        2949..3094
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             3137..4174
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(4172..4191)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4216..4264
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        4918..5297
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5298..5501
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5452..5472
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5498..5522
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             5604..5633
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     polyA_signal    6709..6843
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6733..6752)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6787..6806
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(6986..7008)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      7568..9357
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
ORIGIN
        1 tgctcctgtt ccaccgtggg tccctttgca gccaatgcaa cttggacgtt tttggggtct
       61 ccggacacca tctctatgtc ttggccctga tcctgagccg cccggggctc ctggtcttcc
      121 gcctcctcgt cctcgtcctc ttccccgtcc tcgtccatgg ttatcacccc ctcttctttg
      181 aggtccactg ccgccggagc cttctggtcc agatgtgtct cccttctctc ctaggccatt
      241 tccaggtcct gtacctggcc cctcgtcaga catgattcac actaaaagag atcaatagac
      301 atctttatta gacgacgctc agtgaataca gggagtgcag actcctgccc cctccaacag
      361 cccccccacc ctcatcccct tcatggtcgc tgtcagacag atccaggtct gaaaattccc
      421 catcctccga accatcctcg tcctcatcac caattactcg cagcccggaa aactcccgct
      481 gaacatcctc aagatttgcg tcctgagcct caagccaggc ctcaaattcc tcgtccccct
      541 ttttgctgga cggtagggat ggggattctc gggacccctc ctcttcctct tcaaggtcac
      601 cagacagaga tgctactggg gcaacggaag aaaagctggg tgcggcctgt gaggatcagc
      661 ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg
      721 cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt
      781 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
      841 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
      901 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
      961 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     1021 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     1081 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     1141 tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     1201 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     1261 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     1321 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     1381 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     1441 tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     1501 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     1561 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     1621 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     1681 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     1741 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     1801 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     1861 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     1921 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     1981 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     2041 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg
     2101 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     2161 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     2221 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     2281 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     2341 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     2401 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     2461 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     2521 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttgaa gctgtccctg
     2581 atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc
     2641 ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa
     2701 gacgtagccc agcgcgtcgg ccccgagatg cgccgcgtgc ggctgctgga gatggcggac
     2761 gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga
     2821 ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccctgc ttcatccccg
     2881 tggcccgttg ctcgcgtttg ctggcggtgt ccccggaaga aatatatttg catgtcttta
     2941 gttctatgat gacacaaacc ccgcccagcg tcttgtcatt ggcgaattcg aacacgcaga
     3001 tgcagtcggg gcggcgcggt ccgaggtcca cttcgcatat taaggtgacg cgtgtggcct
     3061 cgaacaccga gcgaccctgc agcgacccgc ttaacagcgt caacagcgtg ccgcagatcc
     3121 cggggggcaa tgagatatga aaaagcctga actcaccgcg acgtctgtcg agaagtttct
     3181 gatcgaaaag ttcgacagcg tctccgacct gatgcagctc tcggagggcg aagaatctcg
     3241 tgctttcagc ttcgatgtag gagggcgtgg atatgtcctg cgggtaaata gctgcgccga
     3301 tggtttctac aaagatcgtt atgtttatcg gcactttgca tcggccgcgc tcccgattcc
     3361 ggaagtgctt gacattgggg aattcagcga gagcctgacc tattgcatct cccgccgtgc
     3421 acagggtgtc acgttgcaag acctgcctga aaccgaactg cccgctgttc tgcagccggt
     3481 cgcggaggcc atggatgcga tcgctgcggc cgatcttagc cagacgagcg ggttcggccc
     3541 attcggaccg caaggaatcg gtcaatacac tacatggcgt gatttcatat gcgcgattgc
     3601 tgatccccat gtgtatcact ggcaaactgt gatggacgac accgtcagtg cgtccgtcgc
     3661 gcaggctctc gatgagctga tgctttgggc cgaggactgc cccgaagtcc ggcacctcgt
     3721 gcacgcggat ttcggctcca acaatgtcct gacggacaat ggccgcataa cagcggtcat
     3781 tgactggagc gaggcgatgt tcggggattc ccaatacgag gtcgccaaca tcttcttctg
     3841 gaggccgtgg ttggcttgta tggagcagca gacgcgctac ttcgagcgga ggcatccgga
     3901 gcttgcagga tcgccgcggc tccgggcgta tatgctccgc attggtcttg accaactcta
     3961 tcagagcttg gttgacggca atttcgatga tgcagcttgg gcgcagggtc gatgcgacgc
     4021 aatcgtccga tccggagccg ggactgtcgg gcgtacacaa atcgcccgca gaagcgcggc
     4081 cgtctggacc gatggctgtg tagaagtact cgccgatagt ggaaaccgac gccccagcac
     4141 tcgtccggat cgggagatgg gggaggctaa ctgaaacacg gaaggagaca ataccggaag
     4201 gaacccgcgc tatgacggca ataaaaagac agaataaaac gcacgggtgt tgggtcgttt
     4261 gttcataaac gcggggttcg gtcccagggc tggcactctg tcgatacccc accgagaccc
     4321 cattggggcc aatacgcccg cgtttcttcc ttttccccac cccacccccc aagttcgggt
     4381 gaaggcccag ggctcgcagc caacgtcggg gcggcaggcc ctgccatagc cactggcccc
     4441 gtgggttagg gacggggtcc cccatgggga atggtttatg gttcgtgggg gttattattt
     4501 tgggcgttgc gtggggtcag gtccacgact ggactgagca gacagaccca tggtttttgg
     4561 atggcctggg catggaccgc atgtactggc gcgacacgaa caccgggcgt ctgtggctgc
     4621 caaacacccc cgacccccaa aaaccaccgc gcggatttct ggcgtgccaa gctagtcgac
     4681 caattctcat gtttgacagc ttatcatcgc agatccgggc aacgttgttg ccattgctgc
     4741 aggcgcagaa ctggtaggta tggaagatct atacattgaa tcaatattgg caattagcca
     4801 tattagtcat tggttatata gcataaatca atattggcta ttggccattg catacgttgt
     4861 atctatatca taatatgtac atttatattg gctcatgtcc aatatgaccg ccatgttgac
     4921 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
     4981 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
     5041 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
     5101 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
     5161 tgtatcatat gccaagtccg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
     5221 attatgccca gtacatgacc ttacgggact ttcctacttg gcagtacatc tacgtattag
     5281 tcatcgctat taccatggtg atgcggtttt ggcagtacac caatgggcgt ggatagcggt
     5341 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
     5401 accaaaatca acgggacttt ccaaaatgtc gtaataaccc cgccccgttg acgcaaatgg
     5461 gcggtaggcg tgtacggtgg gaggtctata taagcagagc tcgtttagtg aaccgtcaga
     5521 tctctagaag ctgggtacca gctgctagcg ccaccatgaa gaccatcatc gccctgagct
     5581 acatcttctg cctggtgttc gccgagcaga aattgatcag cgaggaggac ctgggcagcc
     5641 ctggaggcag ttcaggaggc ggatctcatt ctatgagata tttcttcaca tccgtgtccc
     5701 ggcccggccg cggggagccc cgcttcatcg ccgtgggcta cgtggacgac acgcagttcg
     5761 tgcggttcga cagcgacgcc gcgagccaga agatggagcc gcgggcgccg tggatagagc
     5821 aggaggggcc ggagtattgg gaccaggaga cacggaatat gaaggcccac tcacagactg
     5881 accgagcgaa cctggggacc ctgcgcggct actacaacca gagcgaggac ggttctcaca
     5941 ccatccagat aatgtatggc tgcgacgtgg ggccggacgg gcgcttcctc cgcgggtacc
     6001 ggcaggacgc ctacgacggc aaggattaca tcgccctgaa cgaggacctg cgttcttgga
     6061 ccgcggcgga catggcagct cagatcacca agcgcaagtg ggaggcggtc catgcggcgg
     6121 agcagcggag agtctacctg gagggccggt gcgtggacgg gctccgcaga tacctggaga
     6181 acgggaagga gacgctgcag cgcacggacc cccccaagac acatatgacc caccacccca
     6241 tctctgacca tgaggccacc ctgaggtgct gggccctggg cttctaccct gcggagatca
     6301 cactgacctg gcagcgggat ggggaggacc agacccagga cacggagctc gtggagacca
     6361 ggcctgcagg ggatggaacc ttccagaagt gggcggctgt ggtggtgcct tctggagagg
     6421 agcagagata cacctgccat gtgcagcatg agggtctgcc caagcccctc accctgagat
     6481 gggagctgtc ttcccagccc actataccga ttgttggcat catagccgga cttgtattgc
     6541 tgggtgcggt gattacgggc gccgtcgtcg ctgctgtgat gtggcggcgg aagtcttcag
     6601 acagaaaagg cggatcttat acccaagctg caagctcaga ttcagcacag gggtctgatg
     6661 tgagtctgac agcttgcaaa gtgtgataac tcgaggccgg caaggccgga tccagacatg
     6721 ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt
     6781 atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa
     6841 gttaacaaca acaattgcat tcattttatg tttcaggttc agggggaggt gtgggaggtt
     6901 ttttaaagca agtaaaacct ctacaaatgt ggtatggctg attatgatcc ggctgcctcg
     6961 cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag
     7021 cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg
     7081 gcgggtgtcg gggcgcagcc atgaggtcga ctctagagga tcgatccccg ccccggacga
     7141 actaaacctg actacgacat ctctgcccct tcttcgcggg gcagtgcatg taatcccttc
     7201 agttggttgg tacaacttgc caactgggcc ctgttccaca tgtgacacgg ggggggacca
     7261 aacacaaagg ggttctctga ctgtagttga catccttata aatggatgtg cacatttgcc
     7321 aacactgagt ggctttcatc ctggagcaga ctttgcagtc tgtggactgc aacacaacat
     7381 tgcctttatg tgtaactctt ggctgaagct cttacaccaa tgctggggga catgtacctc
     7441 ccaggggccc aggaagacta cgggaggcta caccaacgtc aatcagaggg gcctgtgtag
     7501 ctaccgataa gcggaccctc aagagggcat tagcaatagt gtttataagg cccccttgtt
     7561 aaccctaaac gggtagcata tgcttcccgg gtagtagtat atactatcca gactaaccct
     7621 aattcaatag catatgttac ccaacgggaa gcatatgcta tcgaattagg gttagtaaaa
     7681 gggtcctaag gaacagcgat atctcccacc ccatgagctg tcacggtttt atttacatgg
     7741 ggtcaggatt ccacgagggt agtgaaccat tttagtcaca agggcagtgg ctgaagatca
     7801 aggagcgggc agtgaactct cctgaatctt cgcctgcttc ttcattctcc ttcgtttagc
     7861 taatagaata actgctgagt tgtgaacagt aaggtgtatg tgaggtgctc gaaaacaagg
     7921 tttcaggtga cgcccccaga ataaaatttg gacggggggt tcagtggtgg cattgtgcta
     7981 tgacaccaat ataaccctca caaacccctt gggcaataaa tactagtgta ggaatgaaac
     8041 attctgaata tctttaacaa tagaaatcca tggggtgggg acaagccgta aagactggat
     8101 gtccatctca cacgaattta tggctatggg caacacataa tcctagtgca atatgatact
     8161 ggggttatta agatgtgtcc caggcaggga ccaagacagg tgaaccatgt tgttacactc
     8221 tatttgtaac aaggggaaag agagtggacg ccgacagcag cggactccac tggttgtctc
     8281 taacaccccc gaaaattaaa cggggctcca cgccaatggg gcccataaac aaagacaagt
     8341 ggccactctt ttttttgaaa ttgtggagtg ggggcacgcg tcagccccca cacgccgccc
     8401 tgcggttttg gactgtaaaa taagggtgta ataacttggc tgattgtaac cccgctaacc
     8461 actgcggtca aaccacttgc ccacaaaacc actaatggca ccccggggaa tacctgcata
     8521 agtaggtggg cgggccaaga taggggcgcg attgctgcga tctggaggac aaattacaca
     8581 cacttgcgcc tgagcgccaa gcacagggtt gttggtcctc atattcacga ggtcgctgag
     8641 agcacggtgg gctaatgttg ccatgggtag catatactac ccaaatatct ggatagcata
     8701 tgctatccta atctatatct gggtagcata ggctatccta atctatatct gggtagcata
     8761 tgctatccta atctatatct gggtagtata tgctatccta atttatatct gggtagcata
     8821 ggctatccta atctatatct gggtagcata tgctatccta atctatatct gggtagtata
     8881 tgctatccta atctgtatcc gggtagcata tgctatccta atagagatta gggtagtata
     8941 tgctatccta atttatatct gggtagcata tactacccaa atatctggat agcatatgct
     9001 atcctaatct atatctgggt agcatatgct atcctaatct atatctgggt agcataggct
     9061 atcctaatct atatctgggt agcatatgct atcctaatct atatctgggt agtatatgct
     9121 atcctaattt atatctgggt agcataggct atcctaatct atatctgggt agcatatgct
     9181 atcctaatct atatctgggt agtatatgct atcctaatct gtatccgggt agcatatgct
     9241 atcctcatgc atatacagtc agcatatgat acccagtagt agagtgggag tgctatcctt
     9301 tgcatatgcc gccacctccc aagggggcgt gaattttcgc tgcttgtcct tttcctgctg
     9361 gttgctccca ttcttaggtg aatttaagga ggccaggcta aagccgtcgc atgtctgatt
     9421 gctcaccagg taaatgtcgc taatgttttc caacgcgaga aggtgttgag cgcggagctg
     9481 agtgacgtga caacatgggt atgcccaatt gccccatgtt gggaggacga aaatggtgac
     9541 aagacagatg gccagaaata
//
