LOCUS       sequence-270581-       10713 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of VC-fused and FLAG-tagged TAPBPR with MHC TM.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 10713)
  AUTHORS   McShan AC, Devlin CA, Overall SA, Park J, Toor JS, Moschidi D, 
            Flores-Solis D, Choi H, Tripathi S, Procko E, Sgourakis NG
  TITLE     Molecular determinants of chaperone interactions on MHC-I for 
            folding and antigen repertoire selection.
  JOURNAL   Proc Natl Acad Sci U S A. 2019 Dec 17;116(51):25602-25613. doi: 
            10.1073/pnas.1915562116. Epub 2019 Dec 3.
  PUBMED    31796585
REFERENCE   2  (bases 1 to 10713)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1915562116"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2019-12-17- 17"; volume: "116"; issue: "51"; pages: 
            "25602-25613"
COMMENT     Sequence Label: pCEP4-FLAG-TAPBPR-TM-VC
FEATURES             Location/Qualifiers
     source          1..10713
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(237..255)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        323..427
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(428..496,497..1288)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(646..665)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1459..2047
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1948..1967
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        2478..2623
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             2666..3703
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(3701..3720)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    3745..3793
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        4447..4826
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4827..5030
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4981..5001
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5027..5051
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      5079..5088
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5163..5186
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             6477..6728
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     6665..6686
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    6751..6885
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6775..6794)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6829..6848
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(7028..7050)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      7610..9399
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
ORIGIN
        1 actcccgctg aacatcctca agatttgcgt cctgagcctc aagccaggcc tcaaattcct
       61 cgtccccctt tttgctggac ggtagggatg gggattctcg ggacccctcc tcttcctctt
      121 caaggtcacc agacagagat gctactgggg caacggaaga aaagctgggt gcggcctgtg
      181 aggatcagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct
      241 cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg
      301 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
      361 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
      421 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
      481 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
      541 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
      601 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
      661 attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
      721 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
      781 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
      841 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
      901 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
      961 cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
     1021 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
     1081 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
     1141 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
     1201 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
     1261 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
     1321 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     1381 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     1441 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     1501 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     1561 tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc
     1621 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     1681 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     1741 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     1801 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     1861 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     1921 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     1981 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     2041 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttgaag
     2101 ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc
     2161 gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa
     2221 cgccagcaag acgtagccca gcgcgtcggc cccgagatgc gccgcgtgcg gctgctggag
     2281 atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc
     2341 aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccctgct
     2401 tcatccccgt ggcccgttgc tcgcgtttgc tggcggtgtc cccggaagaa atatatttgc
     2461 atgtctttag ttctatgatg acacaaaccc cgcccagcgt cttgtcattg gcgaattcga
     2521 acacgcagat gcagtcgggg cggcgcggtc cgaggtccac ttcgcatatt aaggtgacgc
     2581 gtgtggcctc gaacaccgag cgaccctgca gcgacccgct taacagcgtc aacagcgtgc
     2641 cgcagatccc ggggggcaat gagatatgaa aaagcctgaa ctcaccgcga cgtctgtcga
     2701 gaagtttctg atcgaaaagt tcgacagcgt ctccgacctg atgcagctct cggagggcga
     2761 agaatctcgt gctttcagct tcgatgtagg agggcgtgga tatgtcctgc gggtaaatag
     2821 ctgcgccgat ggtttctaca aagatcgtta tgtttatcgg cactttgcat cggccgcgct
     2881 cccgattccg gaagtgcttg acattgggga attcagcgag agcctgacct attgcatctc
     2941 ccgccgtgca cagggtgtca cgttgcaaga cctgcctgaa accgaactgc ccgctgttct
     3001 gcagccggtc gcggaggcca tggatgcgat cgctgcggcc gatcttagcc agacgagcgg
     3061 gttcggccca ttcggaccgc aaggaatcgg tcaatacact acatggcgtg atttcatatg
     3121 cgcgattgct gatccccatg tgtatcactg gcaaactgtg atggacgaca ccgtcagtgc
     3181 gtccgtcgcg caggctctcg atgagctgat gctttgggcc gaggactgcc ccgaagtccg
     3241 gcacctcgtg cacgcggatt tcggctccaa caatgtcctg acggacaatg gccgcataac
     3301 agcggtcatt gactggagcg aggcgatgtt cggggattcc caatacgagg tcgccaacat
     3361 cttcttctgg aggccgtggt tggcttgtat ggagcagcag acgcgctact tcgagcggag
     3421 gcatccggag cttgcaggat cgccgcggct ccgggcgtat atgctccgca ttggtcttga
     3481 ccaactctat cagagcttgg ttgacggcaa tttcgatgat gcagcttggg cgcagggtcg
     3541 atgcgacgca atcgtccgat ccggagccgg gactgtcggg cgtacacaaa tcgcccgcag
     3601 aagcgcggcc gtctggaccg atggctgtgt agaagtactc gccgatagtg gaaaccgacg
     3661 ccccagcact cgtccggatc gggagatggg ggaggctaac tgaaacacgg aaggagacaa
     3721 taccggaagg aacccgcgct atgacggcaa taaaaagaca gaataaaacg cacgggtgtt
     3781 gggtcgtttg ttcataaacg cggggttcgg tcccagggct ggcactctgt cgatacccca
     3841 ccgagacccc attggggcca atacgcccgc gtttcttcct tttccccacc ccacccccca
     3901 agttcgggtg aaggcccagg gctcgcagcc aacgtcgggg cggcaggccc tgccatagcc
     3961 actggccccg tgggttaggg acggggtccc ccatggggaa tggtttatgg ttcgtggggg
     4021 ttattatttt gggcgttgcg tggggtcagg tccacgactg gactgagcag acagacccat
     4081 ggtttttgga tggcctgggc atggaccgca tgtactggcg cgacacgaac accgggcgtc
     4141 tgtggctgcc aaacaccccc gacccccaaa aaccaccgcg cggatttctg gcgtgccaag
     4201 ctagtcgacc aattctcatg tttgacagct tatcatcgca gatccgggca acgttgttgc
     4261 cattgctgca ggcgcagaac tggtaggtat ggaagatcta tacattgaat caatattggc
     4321 aattagccat attagtcatt ggttatatag cataaatcaa tattggctat tggccattgc
     4381 atacgttgta tctatatcat aatatgtaca tttatattgg ctcatgtcca atatgaccgc
     4441 catgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
     4501 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
     4561 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
     4621 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
     4681 tacatcaagt gtatcatatg ccaagtccgc cccctattga cgtcaatgac ggtaaatggc
     4741 ccgcctggca ttatgcccag tacatgacct tacgggactt tcctacttgg cagtacatct
     4801 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacacc aatgggcgtg
     4861 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
     4921 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taataacccc gccccgttga
     4981 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga
     5041 accgtcagat ctctagaagc tgggtaccag ctgctagcgc caccatggcc gtcatggcgc
     5101 cccgaaccct cgtcctgcta ctctcggggg ccctggccct gacccagacc tgggcgggct
     5161 ccgactacaa ggacgatgat gacaagggag gatccaagcc ccacccagca gaggggcagt
     5221 ggcgggcagt ggacgtggtc ctagactgtt tcctggtgaa ggacggtgcg caccgtggag
     5281 ctctcgccag cagtgaggac agggcaaggg cctcccttgt gctgaagcag gtgccagtgc
     5341 tggacgatgg ctccctggag gacttcaccg atttccaagg gggcacactg gcccaagatg
     5401 acccacctat tatctttgag gcctcagtgg acctggtcca gattccccag gccgaggcct
     5461 tgctccatgc tgactgcagt gggaaggagg tgacctgtga gatctcccgc tactttctcc
     5521 agatgacaga gaccactgtt aagacagcag cttggttcat ggccaacgtg caggtctctg
     5581 gagggggacc tagcatctcc ttggtgatga agactcccag ggtcgccaag aatgaggtgc
     5641 tctggcaccc aacgctgaac ttgccactga gcccccaggg gactgtgcga actgcagtgg
     5701 agttccaggt gatgacacag acccaatccc tgagcttcct gctggggtcc tcagcctcct
     5761 tggactgtgg cttctccatg gcaccgggct tggacctcat cagtgtggag tggcgactgc
     5821 agcacaaggg caggggtcag ttggtgtaca gctggaccgc agggcagggg caggctgtgc
     5881 ggaagggcgc taccctggag cctgcacaac tgggcatggc cagggatgcc tccctcaccc
     5941 tgcccggcct cactatacag gacgagggga cctacatttg ccagatcacc acctctctgt
     6001 accgagctca gcagatcatc cagctcaaca tccaagcttc ccctaaagta cgactgagct
     6061 tggcaaacga agctctgctg cccaccctca tctgcgacat tgctggctat taccctctgg
     6121 atgtggtggt gacgtggacc cgagaggagc tgggtggatc cccagcccaa gtctctggtg
     6181 cctccttctc cagcctcagg caaagcgtgg caggcaccta cagcatctcc tcctctctca
     6241 ccgcagaacc tggctctgca ggtgccactt acacctgcca ggtcacacac atctctctgg
     6301 aggagcccct tggggccagc acccaggttg tcccaccaga gcggagatct ggagccggaa
     6361 gtgctatccc catcatgggt atcgttgctg gcctggttgt ccttgcagct gtagtcactg
     6421 gagctgcggt cgctgctgtg ctgtggagaa agaagagctc agatggaggc tctggagaca
     6481 agcagaagaa cggcatcaag gccaacttca agatccgcca caacatcgag gacggcggcg
     6541 tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc
     6601 ccgacaacca ctacctgagc taccagtcca agctgagcaa agaccccaac gagaagcgcg
     6661 atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc
     6721 tgtacaagta actcgaggcc ggcaaggccg gatccagaca tgataagata cattgatgag
     6781 tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat
     6841 gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc
     6901 attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag caagtaaaac
     6961 ctctacaaat gtggtatggc tgattatgat ccggctgcct cgcgcgtttc ggtgatgacg
     7021 gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg
     7081 ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag
     7141 ccatgaggtc gactctagag gatcgatccc cgccccggac gaactaaacc tgactacgac
     7201 atctctgccc cttcttcgcg gggcagtgca tgtaatccct tcagttggtt ggtacaactt
     7261 gccaactggg ccctgttcca catgtgacac ggggggggac caaacacaaa ggggttctct
     7321 gactgtagtt gacatcctta taaatggatg tgcacatttg ccaacactga gtggctttca
     7381 tcctggagca gactttgcag tctgtggact gcaacacaac attgccttta tgtgtaactc
     7441 ttggctgaag ctcttacacc aatgctgggg gacatgtacc tcccaggggc ccaggaagac
     7501 tacgggaggc tacaccaacg tcaatcagag gggcctgtgt agctaccgat aagcggaccc
     7561 tcaagagggc attagcaata gtgtttataa ggcccccttg ttaaccctaa acgggtagca
     7621 tatgcttccc gggtagtagt atatactatc cagactaacc ctaattcaat agcatatgtt
     7681 acccaacggg aagcatatgc tatcgaatta gggttagtaa aagggtccta aggaacagcg
     7741 atatctccca ccccatgagc tgtcacggtt ttatttacat ggggtcagga ttccacgagg
     7801 gtagtgaacc attttagtca caagggcagt ggctgaagat caaggagcgg gcagtgaact
     7861 ctcctgaatc ttcgcctgct tcttcattct ccttcgttta gctaatagaa taactgctga
     7921 gttgtgaaca gtaaggtgta tgtgaggtgc tcgaaaacaa ggtttcaggt gacgccccca
     7981 gaataaaatt tggacggggg gttcagtggt ggcattgtgc tatgacacca atataaccct
     8041 cacaaacccc ttgggcaata aatactagtg taggaatgaa acattctgaa tatctttaac
     8101 aatagaaatc catggggtgg ggacaagccg taaagactgg atgtccatct cacacgaatt
     8161 tatggctatg ggcaacacat aatcctagtg caatatgata ctggggttat taagatgtgt
     8221 cccaggcagg gaccaagaca ggtgaaccat gttgttacac tctatttgta acaaggggaa
     8281 agagagtgga cgccgacagc agcggactcc actggttgtc tctaacaccc ccgaaaatta
     8341 aacggggctc cacgccaatg gggcccataa acaaagacaa gtggccactc ttttttttga
     8401 aattgtggag tgggggcacg cgtcagcccc cacacgccgc cctgcggttt tggactgtaa
     8461 aataagggtg taataacttg gctgattgta accccgctaa ccactgcggt caaaccactt
     8521 gcccacaaaa ccactaatgg caccccgggg aatacctgca taagtaggtg ggcgggccaa
     8581 gataggggcg cgattgctgc gatctggagg acaaattaca cacacttgcg cctgagcgcc
     8641 aagcacaggg ttgttggtcc tcatattcac gaggtcgctg agagcacggt gggctaatgt
     8701 tgccatgggt agcatatact acccaaatat ctggatagca tatgctatcc taatctatat
     8761 ctgggtagca taggctatcc taatctatat ctgggtagca tatgctatcc taatctatat
     8821 ctgggtagta tatgctatcc taatttatat ctgggtagca taggctatcc taatctatat
     8881 ctgggtagca tatgctatcc taatctatat ctgggtagta tatgctatcc taatctgtat
     8941 ccgggtagca tatgctatcc taatagagat tagggtagta tatgctatcc taatttatat
     9001 ctgggtagca tatactaccc aaatatctgg atagcatatg ctatcctaat ctatatctgg
     9061 gtagcatatg ctatcctaat ctatatctgg gtagcatagg ctatcctaat ctatatctgg
     9121 gtagcatatg ctatcctaat ctatatctgg gtagtatatg ctatcctaat ttatatctgg
     9181 gtagcatagg ctatcctaat ctatatctgg gtagcatatg ctatcctaat ctatatctgg
     9241 gtagtatatg ctatcctaat ctgtatccgg gtagcatatg ctatcctcat gcatatacag
     9301 tcagcatatg atacccagta gtagagtggg agtgctatcc tttgcatatg ccgccacctc
     9361 ccaagggggc gtgaattttc gctgcttgtc cttttcctgc tggttgctcc cattcttagg
     9421 tgaatttaag gaggccaggc taaagccgtc gcatgtctga ttgctcacca ggtaaatgtc
     9481 gctaatgttt tccaacgcga gaaggtgttg agcgcggagc tgagtgacgt gacaacatgg
     9541 gtatgcccaa ttgccccatg ttgggaggac gaaaatggtg acaagacaga tggccagaaa
     9601 tacaccaaca gcacgcatga tgtctactgg ggatttattc tttagtgcgg gggaatacac
     9661 ggcttttaat acgattgagg gcgtctccta acaagttaca tcactcctgc ccttcctcac
     9721 cctcatctcc atcacctcct tcatctccgt catctccgtc atcaccctcc gcggcagccc
     9781 cttccaccat aggtggaaac cagggaggca aatctactcc atcgtcaaag ctgcacacag
     9841 tcaccctgat attgcaggta ggagcgggct ttgtcataac aaggtcctta atcgcatcct
     9901 tcaaaacctc agcaaatata tgagtttgta aaaagaccat gaaataacag acaatggact
     9961 cccttagcgg gccaggttgt gggccgggtc caggggccat tccaaagggg agacgactca
    10021 atggtgtaag acgacattgt ggaatagcaa gggcagttcc tcgccttagg ttgtaaaggg
    10081 aggtcttact acctccatat acgaacacac cggcgaccca agttccttcg tcggtagtcc
    10141 tttctacgtg actcctagcc aggagagctc ttaaaccttc tgcaatgttc tcaaatttcg
    10201 ggttggaacc tccttgacca cgatgctttc caaaccaccc tccttttttg cgcctgcctc
    10261 catcaccctg accccggggt ccagtgcttg ggccttctcc tgggtcatct gcggggccct
    10321 gctctatcgc tcccgggggc acgtcaggct caccatctgg gccaccttct tggtggtatt
    10381 caaaataatc ggcttcccct acagggtgga aaaatggcct tctacctgga gggggcctgc
    10441 gcggtggaga cccggatgat gatgactgac tactgggact cctgggcctc ttttctccac
    10501 gtccacgacc tctccccctg gctctttcac gacttccccc cctggctctt tcacgtcctc
    10561 taccccggcg gcctccacta cctcctcgac cccggcctcc actacctcct cgaccccggc
    10621 ctccactgcc tcctcgaccc cggcctccac ctcctgctcc tgcccctcct gctcctgccc
    10681 ctcctcctgc tcctgcccct cctgcccctc ctg
//
