LOCUS       sequence-270584-       10029 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of myc-tagged H2-Dd (Y84C/C121S/A139C mutant).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 10029)
  AUTHORS   McShan AC, Devlin CA, Overall SA, Park J, Toor JS, Moschidi D, 
            Flores-Solis D, Choi H, Tripathi S, Procko E, Sgourakis NG
  TITLE     Molecular determinants of chaperone interactions on MHC-I for 
            folding and antigen repertoire selection.
  JOURNAL   Proc Natl Acad Sci U S A. 2019 Dec 17;116(51):25602-25613. doi: 
            10.1073/pnas.1915562116. Epub 2019 Dec 3.
  PUBMED    31796585
REFERENCE   2  (bases 1 to 10029)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1915562116"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2019-12-17- 17"; volume: "116"; issue: "51"; pages: 
            "25602-25613"
COMMENT     Sequence Label: pCEP4-myc-H2-Dd (Y84C/C121S/A139C)
FEATURES             Location/Qualifiers
     source          1..10029
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(121..139)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        207..311
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(312..380,381..1172)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(530..549)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1343..1931
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1832..1851
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        2362..2507
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             2550..3587
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(3585..3604)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    3629..3677
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        4331..4710
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4711..4914
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4865..4885
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     4911..4935
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             5017..5046
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     polyA_signal    6122..6256
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6146..6165)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6200..6219
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(6399..6421)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      6981..8770
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
ORIGIN
        1 tcttcaaggt caccagacag agatgctact ggggcaacgg aagaaaagct gggtgcggcc
       61 tgtgaggatc agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg
      121 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt
      181 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
      241 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
      301 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
      361 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
      421 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
      481 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
      541 cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc
      601 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
      661 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
      721 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
      781 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
      841 acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
      901 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
      961 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     1021 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     1081 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     1141 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     1201 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     1261 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     1321 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     1381 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     1441 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
     1501 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     1561 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     1621 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     1681 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     1741 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     1801 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     1861 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     1921 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     1981 gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca
     2041 tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg
     2101 cgaacgccag caagacgtag cccagcgcgt cggccccgag atgcgccgcg tgcggctgct
     2161 ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct
     2221 ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccc
     2281 tgcttcatcc ccgtggcccg ttgctcgcgt ttgctggcgg tgtccccgga agaaatatat
     2341 ttgcatgtct ttagttctat gatgacacaa accccgccca gcgtcttgtc attggcgaat
     2401 tcgaacacgc agatgcagtc ggggcggcgc ggtccgaggt ccacttcgca tattaaggtg
     2461 acgcgtgtgg cctcgaacac cgagcgaccc tgcagcgacc cgcttaacag cgtcaacagc
     2521 gtgccgcaga tcccgggggg caatgagata tgaaaaagcc tgaactcacc gcgacgtctg
     2581 tcgagaagtt tctgatcgaa aagttcgaca gcgtctccga cctgatgcag ctctcggagg
     2641 gcgaagaatc tcgtgctttc agcttcgatg taggagggcg tggatatgtc ctgcgggtaa
     2701 atagctgcgc cgatggtttc tacaaagatc gttatgttta tcggcacttt gcatcggccg
     2761 cgctcccgat tccggaagtg cttgacattg gggaattcag cgagagcctg acctattgca
     2821 tctcccgccg tgcacagggt gtcacgttgc aagacctgcc tgaaaccgaa ctgcccgctg
     2881 ttctgcagcc ggtcgcggag gccatggatg cgatcgctgc ggccgatctt agccagacga
     2941 gcgggttcgg cccattcgga ccgcaaggaa tcggtcaata cactacatgg cgtgatttca
     3001 tatgcgcgat tgctgatccc catgtgtatc actggcaaac tgtgatggac gacaccgtca
     3061 gtgcgtccgt cgcgcaggct ctcgatgagc tgatgctttg ggccgaggac tgccccgaag
     3121 tccggcacct cgtgcacgcg gatttcggct ccaacaatgt cctgacggac aatggccgca
     3181 taacagcggt cattgactgg agcgaggcga tgttcgggga ttcccaatac gaggtcgcca
     3241 acatcttctt ctggaggccg tggttggctt gtatggagca gcagacgcgc tacttcgagc
     3301 ggaggcatcc ggagcttgca ggatcgccgc ggctccgggc gtatatgctc cgcattggtc
     3361 ttgaccaact ctatcagagc ttggttgacg gcaatttcga tgatgcagct tgggcgcagg
     3421 gtcgatgcga cgcaatcgtc cgatccggag ccgggactgt cgggcgtaca caaatcgccc
     3481 gcagaagcgc ggccgtctgg accgatggct gtgtagaagt actcgccgat agtggaaacc
     3541 gacgccccag cactcgtccg gatcgggaga tgggggaggc taactgaaac acggaaggag
     3601 acaataccgg aaggaacccg cgctatgacg gcaataaaaa gacagaataa aacgcacggg
     3661 tgttgggtcg tttgttcata aacgcggggt tcggtcccag ggctggcact ctgtcgatac
     3721 cccaccgaga ccccattggg gccaatacgc ccgcgtttct tccttttccc caccccaccc
     3781 cccaagttcg ggtgaaggcc cagggctcgc agccaacgtc ggggcggcag gccctgccat
     3841 agccactggc cccgtgggtt agggacgggg tcccccatgg ggaatggttt atggttcgtg
     3901 ggggttatta ttttgggcgt tgcgtggggt caggtccacg actggactga gcagacagac
     3961 ccatggtttt tggatggcct gggcatggac cgcatgtact ggcgcgacac gaacaccggg
     4021 cgtctgtggc tgccaaacac ccccgacccc caaaaaccac cgcgcggatt tctggcgtgc
     4081 caagctagtc gaccaattct catgtttgac agcttatcat cgcagatccg ggcaacgttg
     4141 ttgccattgc tgcaggcgca gaactggtag gtatggaaga tctatacatt gaatcaatat
     4201 tggcaattag ccatattagt cattggttat atagcataaa tcaatattgg ctattggcca
     4261 ttgcatacgt tgtatctata tcataatatg tacatttata ttggctcatg tccaatatga
     4321 ccgccatgtt gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta
     4381 gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc
     4441 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
     4501 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
     4561 gcagtacatc aagtgtatca tatgccaagt ccgcccccta ttgacgtcaa tgacggtaaa
     4621 tggcccgcct ggcattatgc ccagtacatg accttacggg actttcctac ttggcagtac
     4681 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta caccaatggg
     4741 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     4801 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaataa ccccgccccg
     4861 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta
     4921 gtgaaccgtc agatctctag aagctgggta ccagctgcta gcgccaccat gaagaccatc
     4981 atcgccctga gctacatctt ctgcctggtg ttcgccgagc agaaattgat cagcgaggag
     5041 gacctgggca gccctggagg cagttcagga ggcggatcac actcgctgag gtatttcgtc
     5101 accgccgtgt cccggcccgg cttcggggag ccccggtaca tggaagtcgg ctacgtggac
     5161 aacacggagt tcgtgcgctt cgacagcgac gcggagaatc cgagatatga gccgcgggcg
     5221 cggtggatag agcaggaggg gccggagtat tgggagcggg agacacgaag agccaagggc
     5281 aatgagcaga gtttccgagt ggacctgagg accgcgctgc gctgctacaa ccagagcgcg
     5341 ggcggctctc acacactcca gtggatggct ggctgtgacg tggagtcgga cgggcgcctc
     5401 ctccgcgggt actggcagtt cgcctacgac ggcagcgatt acatcgccct gaacgaagac
     5461 ctgaaaacgt ggacggcggc ggacatgtgc gcgcagatca cccgacgcaa gtgggagcag
     5521 gctggtgctg cagagagaga ccgggcctac ctggagggcg agtgcgtgga gtggctccgc
     5581 agatacctga agaacgggaa tgctacgctg ctgcgcacag atcccccaaa ggcccatgtg
     5641 acccatcacc gcagacctga aggtgatgtc accctgaggt gctgggccct gggcttctac
     5701 cctgctgaca tcaccctgac ctggcagttg aatggggagg agctgaccca ggaaatggag
     5761 cttgtggaga ccaggcctgc aggggatgga accttccaga agtgggcatc tgtggtggtg
     5821 cctcttggga aggagcagaa gtacacatgc catgtggaac atgaggggct gcctgagccc
     5881 ctcaccctga gatggggcaa ggaggaaccc ccatcctcaa caaagactaa tactgttatt
     5941 atcgcagtac cagtagtttt gggtgcagta gtaattttgg gagccgtaat ggcatttgtt
     6001 atgaagaggc ggcgcaacac tggtgggaag ggcggagact atgctttggc acccggcagc
     6061 cagtcctctg acatgtcctt gcctgactgt aaggtctgat aactcgaggc cggcaaggcc
     6121 ggatccagac atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg
     6181 aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag
     6241 ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga
     6301 ggtgtgggag gttttttaaa gcaagtaaaa cctctacaaa tgtggtatgg ctgattatga
     6361 tccggctgcc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg
     6421 gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg
     6481 tcagcgggtg ttggcgggtg tcggggcgca gccatgaggt cgactctaga ggatcgatcc
     6541 ccgccccgga cgaactaaac ctgactacga catctctgcc ccttcttcgc ggggcagtgc
     6601 atgtaatccc ttcagttggt tggtacaact tgccaactgg gccctgttcc acatgtgaca
     6661 cgggggggga ccaaacacaa aggggttctc tgactgtagt tgacatcctt ataaatggat
     6721 gtgcacattt gccaacactg agtggctttc atcctggagc agactttgca gtctgtggac
     6781 tgcaacacaa cattgccttt atgtgtaact cttggctgaa gctcttacac caatgctggg
     6841 ggacatgtac ctcccagggg cccaggaaga ctacgggagg ctacaccaac gtcaatcaga
     6901 ggggcctgtg tagctaccga taagcggacc ctcaagaggg cattagcaat agtgtttata
     6961 aggccccctt gttaacccta aacgggtagc atatgcttcc cgggtagtag tatatactat
     7021 ccagactaac cctaattcaa tagcatatgt tacccaacgg gaagcatatg ctatcgaatt
     7081 agggttagta aaagggtcct aaggaacagc gatatctccc accccatgag ctgtcacggt
     7141 tttatttaca tggggtcagg attccacgag ggtagtgaac cattttagtc acaagggcag
     7201 tggctgaaga tcaaggagcg ggcagtgaac tctcctgaat cttcgcctgc ttcttcattc
     7261 tccttcgttt agctaataga ataactgctg agttgtgaac agtaaggtgt atgtgaggtg
     7321 ctcgaaaaca aggtttcagg tgacgccccc agaataaaat ttggacgggg ggttcagtgg
     7381 tggcattgtg ctatgacacc aatataaccc tcacaaaccc cttgggcaat aaatactagt
     7441 gtaggaatga aacattctga atatctttaa caatagaaat ccatggggtg gggacaagcc
     7501 gtaaagactg gatgtccatc tcacacgaat ttatggctat gggcaacaca taatcctagt
     7561 gcaatatgat actggggtta ttaagatgtg tcccaggcag ggaccaagac aggtgaacca
     7621 tgttgttaca ctctatttgt aacaagggga aagagagtgg acgccgacag cagcggactc
     7681 cactggttgt ctctaacacc cccgaaaatt aaacggggct ccacgccaat ggggcccata
     7741 aacaaagaca agtggccact cttttttttg aaattgtgga gtgggggcac gcgtcagccc
     7801 ccacacgccg ccctgcggtt ttggactgta aaataagggt gtaataactt ggctgattgt
     7861 aaccccgcta accactgcgg tcaaaccact tgcccacaaa accactaatg gcaccccggg
     7921 gaatacctgc ataagtaggt gggcgggcca agataggggc gcgattgctg cgatctggag
     7981 gacaaattac acacacttgc gcctgagcgc caagcacagg gttgttggtc ctcatattca
     8041 cgaggtcgct gagagcacgg tgggctaatg ttgccatggg tagcatatac tacccaaata
     8101 tctggatagc atatgctatc ctaatctata tctgggtagc ataggctatc ctaatctata
     8161 tctgggtagc atatgctatc ctaatctata tctgggtagt atatgctatc ctaatttata
     8221 tctgggtagc ataggctatc ctaatctata tctgggtagc atatgctatc ctaatctata
     8281 tctgggtagt atatgctatc ctaatctgta tccgggtagc atatgctatc ctaatagaga
     8341 ttagggtagt atatgctatc ctaatttata tctgggtagc atatactacc caaatatctg
     8401 gatagcatat gctatcctaa tctatatctg ggtagcatat gctatcctaa tctatatctg
     8461 ggtagcatag gctatcctaa tctatatctg ggtagcatat gctatcctaa tctatatctg
     8521 ggtagtatat gctatcctaa tttatatctg ggtagcatag gctatcctaa tctatatctg
     8581 ggtagcatat gctatcctaa tctatatctg ggtagtatat gctatcctaa tctgtatccg
     8641 ggtagcatat gctatcctca tgcatataca gtcagcatat gatacccagt agtagagtgg
     8701 gagtgctatc ctttgcatat gccgccacct cccaaggggg cgtgaatttt cgctgcttgt
     8761 ccttttcctg ctggttgctc ccattcttag gtgaatttaa ggaggccagg ctaaagccgt
     8821 cgcatgtctg attgctcacc aggtaaatgt cgctaatgtt ttccaacgcg agaaggtgtt
     8881 gagcgcggag ctgagtgacg tgacaacatg ggtatgccca attgccccat gttgggagga
     8941 cgaaaatggt gacaagacag atggccagaa atacaccaac agcacgcatg atgtctactg
     9001 gggatttatt ctttagtgcg ggggaataca cggcttttaa tacgattgag ggcgtctcct
     9061 aacaagttac atcactcctg cccttcctca ccctcatctc catcacctcc ttcatctccg
     9121 tcatctccgt catcaccctc cgcggcagcc ccttccacca taggtggaaa ccagggaggc
     9181 aaatctactc catcgtcaaa gctgcacaca gtcaccctga tattgcaggt aggagcgggc
     9241 tttgtcataa caaggtcctt aatcgcatcc ttcaaaacct cagcaaatat atgagtttgt
     9301 aaaaagacca tgaaataaca gacaatggac tcccttagcg ggccaggttg tgggccgggt
     9361 ccaggggcca ttccaaaggg gagacgactc aatggtgtaa gacgacattg tggaatagca
     9421 agggcagttc ctcgccttag gttgtaaagg gaggtcttac tacctccata tacgaacaca
     9481 ccggcgaccc aagttccttc gtcggtagtc ctttctacgt gactcctagc caggagagct
     9541 cttaaacctt ctgcaatgtt ctcaaatttc gggttggaac ctccttgacc acgatgcttt
     9601 ccaaaccacc ctcctttttt gcgcctgcct ccatcaccct gaccccgggg tccagtgctt
     9661 gggccttctc ctgggtcatc tgcggggccc tgctctatcg ctcccggggg cacgtcaggc
     9721 tcaccatctg ggccaccttc ttggtggtat tcaaaataat cggcttcccc tacagggtgg
     9781 aaaaatggcc ttctacctgg agggggcctg cgcggtggag acccggatga tgatgactga
     9841 ctactgggac tcctgggcct cttttctcca cgtccacgac ctctccccct ggctctttca
     9901 cgacttcccc ccctggctct ttcacgtcct ctaccccggc ggcctccact acctcctcga
     9961 ccccggcctc cactacctcc tcgaccccgg cctccactgc ctcctcgacc ccggcctcca
    10021 cctcctgct
//
