LOCUS       sequence-270589-        8837 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of VN-fused and myc-tagged HLA-B*15:01.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 8837)
  AUTHORS   McShan AC, Devlin CA, Overall SA, Park J, Toor JS, Moschidi D, 
            Flores-Solis D, Choi H, Tripathi S, Procko E, Sgourakis NG
  TITLE     Molecular determinants of chaperone interactions on MHC-I for 
            folding and antigen repertoire selection.
  JOURNAL   Proc Natl Acad Sci U S A. 2019 Dec 17;116(51):25602-25613. doi: 
            10.1073/pnas.1915562116. Epub 2019 Dec 3.
  PUBMED    31796585
REFERENCE   2  (bases 1 to 8837)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1915562116"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2019-12-17- 17"; volume: "116"; issue: "51"; pages: 
            "25602-25613"
COMMENT     Sequence Label: pCEP4-myc-HLA-B15-VN
FEATURES             Location/Qualifiers
     source          1..8837
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(688..706)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        774..878
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(879..947,948..1739)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1097..1116)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1910..2498
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2399..2418
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        2929..3074
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             3117..4154
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(4152..4171)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4196..4244
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        4898..5277
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5278..5481
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5432..5452
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5478..5502
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             5584..5613
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             join(6661..6663,6664..7122)
                     /codon_start=1
                     /product="improved N-terminal fragment of mVenus for use in
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN155(I152L)
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYLTA"
     primer_bind     complement(6703..6724)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6964..6983)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     polyA_signal    7145..7279
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7169..7188)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7223..7242
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(7422..7444)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 tccctttgca gccaatgcaa cttggacgtt tttggggtct ccggacacca tctctatgtc
       61 ttggccctga tcctgagccg cccggggctc ctggtcttcc gcctcctcgt cctcgtcctc
      121 ttccccgtcc tcgtccatgg ttatcacccc ctcttctttg aggtccactg ccgccggagc
      181 cttctggtcc agatgtgtct cccttctctc ctaggccatt tccaggtcct gtacctggcc
      241 cctcgtcaga catgattcac actaaaagag atcaatagac atctttatta gacgacgctc
      301 agtgaataca gggagtgcag actcctgccc cctccaacag cccccccacc ctcatcccct
      361 tcatggtcgc tgtcagacag atccaggtct gaaaattccc catcctccga accatcctcg
      421 tcctcatcac caattactcg cagcccggaa aactcccgct gaacatcctc aagatttgcg
      481 tcctgagcct caagccaggc ctcaaattcc tcgtccccct ttttgctgga cggtagggat
      541 ggggattctc gggacccctc ctcttcctct tcaaggtcac cagacagaga tgctactggg
      601 gcaacggaag aaaagctggg tgcggcctgt gaggatcagc ttatcgatga taagctgtca
      661 aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg
      721 tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa
      781 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
      841 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
      901 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
      961 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     1021 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     1081 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc
     1141 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     1201 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     1261 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     1321 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     1381 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt
     1441 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     1501 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     1561 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     1621 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     1681 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     1741 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     1801 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     1861 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     1921 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     1981 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     2041 agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     2101 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     2161 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     2221 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     2281 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     2341 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     2401 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     2461 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     2521 tacggttcct ggccttttgc tggccttgaa gctgtccctg atggtcgtca tctacctgcc
     2581 tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa
     2641 tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg
     2701 ccccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca
     2761 agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt
     2821 ggtgaatccg ttagcgaggt gccgccctgc ttcatccccg tggcccgttg ctcgcgtttg
     2881 ctggcggtgt ccccggaaga aatatatttg catgtcttta gttctatgat gacacaaacc
     2941 ccgcccagcg tcttgtcatt ggcgaattcg aacacgcaga tgcagtcggg gcggcgcggt
     3001 ccgaggtcca cttcgcatat taaggtgacg cgtgtggcct cgaacaccga gcgaccctgc
     3061 agcgacccgc ttaacagcgt caacagcgtg ccgcagatcc cggggggcaa tgagatatga
     3121 aaaagcctga actcaccgcg acgtctgtcg agaagtttct gatcgaaaag ttcgacagcg
     3181 tctccgacct gatgcagctc tcggagggcg aagaatctcg tgctttcagc ttcgatgtag
     3241 gagggcgtgg atatgtcctg cgggtaaata gctgcgccga tggtttctac aaagatcgtt
     3301 atgtttatcg gcactttgca tcggccgcgc tcccgattcc ggaagtgctt gacattgggg
     3361 aattcagcga gagcctgacc tattgcatct cccgccgtgc acagggtgtc acgttgcaag
     3421 acctgcctga aaccgaactg cccgctgttc tgcagccggt cgcggaggcc atggatgcga
     3481 tcgctgcggc cgatcttagc cagacgagcg ggttcggccc attcggaccg caaggaatcg
     3541 gtcaatacac tacatggcgt gatttcatat gcgcgattgc tgatccccat gtgtatcact
     3601 ggcaaactgt gatggacgac accgtcagtg cgtccgtcgc gcaggctctc gatgagctga
     3661 tgctttgggc cgaggactgc cccgaagtcc ggcacctcgt gcacgcggat ttcggctcca
     3721 acaatgtcct gacggacaat ggccgcataa cagcggtcat tgactggagc gaggcgatgt
     3781 tcggggattc ccaatacgag gtcgccaaca tcttcttctg gaggccgtgg ttggcttgta
     3841 tggagcagca gacgcgctac ttcgagcgga ggcatccgga gcttgcagga tcgccgcggc
     3901 tccgggcgta tatgctccgc attggtcttg accaactcta tcagagcttg gttgacggca
     3961 atttcgatga tgcagcttgg gcgcagggtc gatgcgacgc aatcgtccga tccggagccg
     4021 ggactgtcgg gcgtacacaa atcgcccgca gaagcgcggc cgtctggacc gatggctgtg
     4081 tagaagtact cgccgatagt ggaaaccgac gccccagcac tcgtccggat cgggagatgg
     4141 gggaggctaa ctgaaacacg gaaggagaca ataccggaag gaacccgcgc tatgacggca
     4201 ataaaaagac agaataaaac gcacgggtgt tgggtcgttt gttcataaac gcggggttcg
     4261 gtcccagggc tggcactctg tcgatacccc accgagaccc cattggggcc aatacgcccg
     4321 cgtttcttcc ttttccccac cccacccccc aagttcgggt gaaggcccag ggctcgcagc
     4381 caacgtcggg gcggcaggcc ctgccatagc cactggcccc gtgggttagg gacggggtcc
     4441 cccatgggga atggtttatg gttcgtgggg gttattattt tgggcgttgc gtggggtcag
     4501 gtccacgact ggactgagca gacagaccca tggtttttgg atggcctggg catggaccgc
     4561 atgtactggc gcgacacgaa caccgggcgt ctgtggctgc caaacacccc cgacccccaa
     4621 aaaccaccgc gcggatttct ggcgtgccaa gctagtcgac caattctcat gtttgacagc
     4681 ttatcatcgc agatccgggc aacgttgttg ccattgctgc aggcgcagaa ctggtaggta
     4741 tggaagatct atacattgaa tcaatattgg caattagcca tattagtcat tggttatata
     4801 gcataaatca atattggcta ttggccattg catacgttgt atctatatca taatatgtac
     4861 atttatattg gctcatgtcc aatatgaccg ccatgttgac attgattatt gactagttat
     4921 taatagtaat caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca
     4981 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca
     5041 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg
     5101 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtccg
     5161 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc
     5221 ttacgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg
     5281 atgcggtttt ggcagtacac caatgggcgt ggatagcggt ttgactcacg gggatttcca
     5341 agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt
     5401 ccaaaatgtc gtaataaccc cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg
     5461 gaggtctata taagcagagc tcgtttagtg aaccgtcaga tctctagaag ctgggtacca
     5521 gctgctagcg ccaccatgaa gaccatcatc gccctgagct acatcttctg cctggtgttc
     5581 gccgagcaga aattgatcag cgaggaggac ctgggcagcc ctggaggcag ttcaggaggc
     5641 ggatctcatt ctatgagata tttctacacc gccatgtccc ggcccggccg cggggagccc
     5701 cgcttcatcg cagtgggcta cgtggacgac acccagttcg tgaggttcga cagcgacgcc
     5761 gcgagtccga ggatggcgcc ccgggcgcca tggatagagc aggaggggcc ggagtattgg
     5821 gaccgggaga cacagatctc caagaccaac acacagactt accgagagag cctgcggaac
     5881 ctgcgcggct actacaacca gagcgaggcc gggtctcaca ccctccagag gatgtacggc
     5941 tgcgacgtgg ggccggacgg gcgcctcctc cgcgggcatg accagtccgc ctacgacggc
     6001 aaggattaca tcgccctgaa cgaggacctg agctcctgga ccgcggcgga cacggcggct
     6061 cagatcaccc agcgcaagtg ggaggcggcc cgtgaggcgg agcagtggag agcctacctg
     6121 gagggcctgt gcgtggagtg gctccgcaga tacctggaga acgggaagga gacgctgcag
     6181 cgcgcggacc ccccaaagac acatgtgacc caccacccca tctctgacca tgaggccacc
     6241 ctgaggtgct gggccctggg cttctaccct gcggagatca cactgacctg gcagcgggat
     6301 ggcgaggacc aaactcagga caccgagctt gtggagacca gaccagcagg agatagaacc
     6361 ttccagaagt gggcagctgt ggtggtgcct tctggagaag agcagagata cacatgccat
     6421 gtacagcatg aggggctgcc gaagcccctc accctgagat gggagccatc ttcccagtcc
     6481 acgataccca tcgtagggat cgttgcggga ctcgccgtct tggctgtggt agtgatcggc
     6541 gcggtcgttg caactgtaat gtgtcggcga aaatcatctg gtggaaaggg cggttcatat
     6601 tcccaagctg catcctctga ttcagcccag gggtccgatg tgtcattgac agcaggaggc
     6661 gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc
     6721 gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc
     6781 aagctgaccc tgaagctgat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc
     6841 gtgaccaccc tgggctacgg cctgcagtgc ttcgcccgct accccgacca catgaagcag
     6901 cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc
     6961 aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg
     7021 aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag
     7081 ctggagtaca actacaacag ccacaacgtc tatctgaccg cctaactcga ggccggcaag
     7141 gccggatcca gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca
     7201 gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat
     7261 aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg
     7321 ggaggtgtgg gaggtttttt aaagcaagta aaacctctac aaatgtggta tggctgatta
     7381 tgatccggct gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc
     7441 ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc
     7501 gcgtcagcgg gtgttggcgg gtgtcggggc gcagccatga ggtcgactct agaggatcga
     7561 tccccgcccc ggacgaacta aacctgacta cgacatctct gccccttctt cgcggggcag
     7621 tgcatgtaat cccttcagtt ggttggtaca acttgccaac tgggccctgt tccacatgtg
     7681 acacgggggg ggaccaaaca caaaggggtt ctctgactgt agttgacatc cttataaatg
     7741 gatgtgcaca tttgccaaca ctgagtggct ttcatcctgg agcagacttt gcagtctgtg
     7801 gactgcaaca caacattgcc tttatgtgta actcttggct gaagctctta caccaatgct
     7861 gggggacatg tacctcccag gggcccagga agactacggg aggctacacc aacgtcaatc
     7921 agaggggcct gtgtagctac cgataagcgg accctcaaga gggcattagc aatagtgttt
     7981 ataaggcccc cttgttaacc ctaaacgggt agcatatgct tcccgggtag tagtatatac
     8041 tatccagact aaccctaatt caatagcata tgttacccaa cgggaagcat atgctatcga
     8101 attagggtta gtaaaagggt cctaaggaac agcgatatct cccaccccat gagctgtcac
     8161 ggttttattt acatggggtc aggattccac gagggtagtg aaccatttta gtcacaaggg
     8221 cagtggctga agatcaagga gcgggcagtg aactctcctg aatcttcgcc tgcttcttca
     8281 ttctccttcg tttagctaat agaataactg ctgagttgtg aacagtaagg tgtatgtgag
     8341 gtgctcgaaa acaaggtttc aggtgacgcc cccagaataa aatttggacg gggggttcag
     8401 tggtggcatt gtgctatgac accaatataa ccctcacaaa ccccttgggc aataaatact
     8461 agtgtaggaa tgaaacattc tgaatatctt taacaataga aatccatggg gtggggacaa
     8521 gccgtaaaga ctggatgtcc atctcacacg aatttatggc tatgggcaac acataatcct
     8581 agtgcaatat gatactgggg ttattaagat gtgtcccagg cagggaccaa gacaggtgaa
     8641 ccatgttgtt acactctatt tgtaacaagg ggaaagagag tggacgccga cagcagcgga
     8701 ctccactggt tgtctctaac acccccgaaa attaaacggg gctccacgcc aatggggccc
     8761 ataaacaaag acaagtggcc actctttttt ttgaaattgt ggagtggggg cacgcgtcag
     8821 cccccacacg ccgccct
//
