LOCUS       sequence-270591-        9670 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of myc-tagged H2-Dd.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 9670)
  AUTHORS   McShan AC, Devlin CA, Overall SA, Park J, Toor JS, Moschidi D, 
            Flores-Solis D, Choi H, Tripathi S, Procko E, Sgourakis NG
  TITLE     Molecular determinants of chaperone interactions on MHC-I for 
            folding and antigen repertoire selection.
  JOURNAL   Proc Natl Acad Sci U S A. 2019 Dec 17;116(51):25602-25613. doi: 
            10.1073/pnas.1915562116. Epub 2019 Dec 3.
  PUBMED    31796585
REFERENCE   2  (bases 1 to 9670)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1915562116"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2019-12-17- 17"; volume: "116"; issue: "51"; pages: 
            "25602-25613"
COMMENT     Sequence Label: pCEP4-myc-H2-Dd
FEATURES             Location/Qualifiers
     source          1..9670
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(724..742)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        810..914
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(915..983,984..1775)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1133..1152)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1946..2534
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2435..2454
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        2965..3110
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             3153..4190
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(4188..4207)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4232..4280
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        4934..5313
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5314..5517
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5468..5488
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5514..5538
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             5620..5649
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     polyA_signal    6725..6859
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6749..6768)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6803..6822
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(7002..7024)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      7584..9373
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
ORIGIN
        1 ctcccgctcc tgctcctgct cctgttccac cgtgggtccc tttgcagcca atgcaacttg
       61 gacgtttttg gggtctccgg acaccatctc tatgtcttgg ccctgatcct gagccgcccg
      121 gggctcctgg tcttccgcct cctcgtcctc gtcctcttcc ccgtcctcgt ccatggttat
      181 caccccctct tctttgaggt ccactgccgc cggagccttc tggtccagat gtgtctccct
      241 tctctcctag gccatttcca ggtcctgtac ctggcccctc gtcagacatg attcacacta
      301 aaagagatca atagacatct ttattagacg acgctcagtg aatacaggga gtgcagactc
      361 ctgccccctc caacagcccc cccaccctca tccccttcat ggtcgctgtc agacagatcc
      421 aggtctgaaa attccccatc ctccgaacca tcctcgtcct catcaccaat tactcgcagc
      481 ccggaaaact cccgctgaac atcctcaaga tttgcgtcct gagcctcaag ccaggcctca
      541 aattcctcgt cccccttttt gctggacggt agggatgggg attctcggga cccctcctct
      601 tcctcttcaa ggtcaccaga cagagatgct actggggcaa cggaagaaaa gctgggtgcg
      661 gcctgtgagg atcagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa
      721 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga
      781 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
      841 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
      901 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
      961 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     1021 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     1081 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     1141 gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt
     1201 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     1261 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     1321 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     1381 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     1441 gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac
     1501 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     1561 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     1621 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     1681 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     1741 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     1801 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     1861 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     1921 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     1981 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     2041 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag
     2101 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     2161 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     2221 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     2281 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     2341 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     2401 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     2461 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     2521 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     2581 cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg
     2641 gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg
     2701 tcgcgaacgc cagcaagacg tagcccagcg cgtcggcccc gagatgcgcc gcgtgcggct
     2761 gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt
     2821 tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg
     2881 ccctgcttca tccccgtggc ccgttgctcg cgtttgctgg cggtgtcccc ggaagaaata
     2941 tatttgcatg tctttagttc tatgatgaca caaaccccgc ccagcgtctt gtcattggcg
     3001 aattcgaaca cgcagatgca gtcggggcgg cgcggtccga ggtccacttc gcatattaag
     3061 gtgacgcgtg tggcctcgaa caccgagcga ccctgcagcg acccgcttaa cagcgtcaac
     3121 agcgtgccgc agatcccggg gggcaatgag atatgaaaaa gcctgaactc accgcgacgt
     3181 ctgtcgagaa gtttctgatc gaaaagttcg acagcgtctc cgacctgatg cagctctcgg
     3241 agggcgaaga atctcgtgct ttcagcttcg atgtaggagg gcgtggatat gtcctgcggg
     3301 taaatagctg cgccgatggt ttctacaaag atcgttatgt ttatcggcac tttgcatcgg
     3361 ccgcgctccc gattccggaa gtgcttgaca ttggggaatt cagcgagagc ctgacctatt
     3421 gcatctcccg ccgtgcacag ggtgtcacgt tgcaagacct gcctgaaacc gaactgcccg
     3481 ctgttctgca gccggtcgcg gaggccatgg atgcgatcgc tgcggccgat cttagccaga
     3541 cgagcgggtt cggcccattc ggaccgcaag gaatcggtca atacactaca tggcgtgatt
     3601 tcatatgcgc gattgctgat ccccatgtgt atcactggca aactgtgatg gacgacaccg
     3661 tcagtgcgtc cgtcgcgcag gctctcgatg agctgatgct ttgggccgag gactgccccg
     3721 aagtccggca cctcgtgcac gcggatttcg gctccaacaa tgtcctgacg gacaatggcc
     3781 gcataacagc ggtcattgac tggagcgagg cgatgttcgg ggattcccaa tacgaggtcg
     3841 ccaacatctt cttctggagg ccgtggttgg cttgtatgga gcagcagacg cgctacttcg
     3901 agcggaggca tccggagctt gcaggatcgc cgcggctccg ggcgtatatg ctccgcattg
     3961 gtcttgacca actctatcag agcttggttg acggcaattt cgatgatgca gcttgggcgc
     4021 agggtcgatg cgacgcaatc gtccgatccg gagccgggac tgtcgggcgt acacaaatcg
     4081 cccgcagaag cgcggccgtc tggaccgatg gctgtgtaga agtactcgcc gatagtggaa
     4141 accgacgccc cagcactcgt ccggatcggg agatggggga ggctaactga aacacggaag
     4201 gagacaatac cggaaggaac ccgcgctatg acggcaataa aaagacagaa taaaacgcac
     4261 gggtgttggg tcgtttgttc ataaacgcgg ggttcggtcc cagggctggc actctgtcga
     4321 taccccaccg agaccccatt ggggccaata cgcccgcgtt tcttcctttt ccccacccca
     4381 ccccccaagt tcgggtgaag gcccagggct cgcagccaac gtcggggcgg caggccctgc
     4441 catagccact ggccccgtgg gttagggacg gggtccccca tggggaatgg tttatggttc
     4501 gtgggggtta ttattttggg cgttgcgtgg ggtcaggtcc acgactggac tgagcagaca
     4561 gacccatggt ttttggatgg cctgggcatg gaccgcatgt actggcgcga cacgaacacc
     4621 gggcgtctgt ggctgccaaa cacccccgac ccccaaaaac caccgcgcgg atttctggcg
     4681 tgccaagcta gtcgaccaat tctcatgttt gacagcttat catcgcagat ccgggcaacg
     4741 ttgttgccat tgctgcaggc gcagaactgg taggtatgga agatctatac attgaatcaa
     4801 tattggcaat tagccatatt agtcattggt tatatagcat aaatcaatat tggctattgg
     4861 ccattgcata cgttgtatct atatcataat atgtacattt atattggctc atgtccaata
     4921 tgaccgccat gttgacattg attattgact agttattaat agtaatcaat tacggggtca
     4981 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct
     5041 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta
     5101 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
     5161 ttggcagtac atcaagtgta tcatatgcca agtccgcccc ctattgacgt caatgacggt
     5221 aaatggcccg cctggcatta tgcccagtac atgaccttac gggactttcc tacttggcag
     5281 tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacaccaat
     5341 gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat
     5401 gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa taaccccgcc
     5461 ccgttgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt
     5521 ttagtgaacc gtcagatctc tagaagctgg gtaccagctg ctagcgccac catgaagacc
     5581 atcatcgccc tgagctacat cttctgcctg gtgttcgccg agcagaaatt gatcagcgag
     5641 gaggacctgg gcagccctgg aggcagttca ggaggcggat cacactcgct gaggtatttc
     5701 gtcaccgccg tgtcccggcc cggcttcggg gagccccggt acatggaagt cggctacgtg
     5761 gacaacacgg agttcgtgcg cttcgacagc gacgcggaga atccgagata tgagccgcgg
     5821 gcgcggtgga tagagcagga ggggccggag tattgggagc gggagacacg aagagccaag
     5881 ggcaatgagc agagtttccg agtggacctg aggaccgcgc tgcgctacta caaccagagc
     5941 gcgggcggct ctcacacact ccagtggatg gctggctgtg acgtggagtc ggacgggcgc
     6001 ctcctccgcg ggtactggca gttcgcctac gacggcagcg attacatcgc cctgaacgaa
     6061 gacctgaaaa cgtggacggc ggcggacatg gcggcgcaga tcacccgacg caagtgggag
     6121 caggctggtg ctgcagagag agaccgggcc tacctggagg gcgagtgcgt ggagtggctc
     6181 cgcagatacc tgaagaacgg gaatgctacg ctgctgcgca cagatccccc aaaggcccat
     6241 gtgacccatc accgcagacc tgaaggtgat gtcaccctga ggtgctgggc cctgggcttc
     6301 taccctgctg acatcaccct gacctggcag ttgaatgggg aggagctgac ccaggaaatg
     6361 gagcttgtgg agaccaggcc tgcaggggat ggaaccttcc agaagtgggc atctgtggtg
     6421 gtgcctcttg ggaaggagca gaagtacaca tgccatgtgg aacatgaggg gctgcctgag
     6481 cccctcaccc tgagatgggg caaggaggaa cccccatcct caacaaagac taatactgtt
     6541 attatcgcag taccagtagt tttgggtgca gtagtaattt tgggagccgt aatggcattt
     6601 gttatgaaga ggcggcgcaa cactggtggg aagggcggag actatgcttt ggcacccggc
     6661 agccagtcct ctgacatgtc cttgcctgac tgtaaggtct gataactcga ggccggcaag
     6721 gccggatcca gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca
     6781 gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat
     6841 aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg
     6901 ggaggtgtgg gaggtttttt aaagcaagta aaacctctac aaatgtggta tggctgatta
     6961 tgatccggct gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc
     7021 ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc
     7081 gcgtcagcgg gtgttggcgg gtgtcggggc gcagccatga ggtcgactct agaggatcga
     7141 tccccgcccc ggacgaacta aacctgacta cgacatctct gccccttctt cgcggggcag
     7201 tgcatgtaat cccttcagtt ggttggtaca acttgccaac tgggccctgt tccacatgtg
     7261 acacgggggg ggaccaaaca caaaggggtt ctctgactgt agttgacatc cttataaatg
     7321 gatgtgcaca tttgccaaca ctgagtggct ttcatcctgg agcagacttt gcagtctgtg
     7381 gactgcaaca caacattgcc tttatgtgta actcttggct gaagctctta caccaatgct
     7441 gggggacatg tacctcccag gggcccagga agactacggg aggctacacc aacgtcaatc
     7501 agaggggcct gtgtagctac cgataagcgg accctcaaga gggcattagc aatagtgttt
     7561 ataaggcccc cttgttaacc ctaaacgggt agcatatgct tcccgggtag tagtatatac
     7621 tatccagact aaccctaatt caatagcata tgttacccaa cgggaagcat atgctatcga
     7681 attagggtta gtaaaagggt cctaaggaac agcgatatct cccaccccat gagctgtcac
     7741 ggttttattt acatggggtc aggattccac gagggtagtg aaccatttta gtcacaaggg
     7801 cagtggctga agatcaagga gcgggcagtg aactctcctg aatcttcgcc tgcttcttca
     7861 ttctccttcg tttagctaat agaataactg ctgagttgtg aacagtaagg tgtatgtgag
     7921 gtgctcgaaa acaaggtttc aggtgacgcc cccagaataa aatttggacg gggggttcag
     7981 tggtggcatt gtgctatgac accaatataa ccctcacaaa ccccttgggc aataaatact
     8041 agtgtaggaa tgaaacattc tgaatatctt taacaataga aatccatggg gtggggacaa
     8101 gccgtaaaga ctggatgtcc atctcacacg aatttatggc tatgggcaac acataatcct
     8161 agtgcaatat gatactgggg ttattaagat gtgtcccagg cagggaccaa gacaggtgaa
     8221 ccatgttgtt acactctatt tgtaacaagg ggaaagagag tggacgccga cagcagcgga
     8281 ctccactggt tgtctctaac acccccgaaa attaaacggg gctccacgcc aatggggccc
     8341 ataaacaaag acaagtggcc actctttttt ttgaaattgt ggagtggggg cacgcgtcag
     8401 cccccacacg ccgccctgcg gttttggact gtaaaataag ggtgtaataa cttggctgat
     8461 tgtaaccccg ctaaccactg cggtcaaacc acttgcccac aaaaccacta atggcacccc
     8521 ggggaatacc tgcataagta ggtgggcggg ccaagatagg ggcgcgattg ctgcgatctg
     8581 gaggacaaat tacacacact tgcgcctgag cgccaagcac agggttgttg gtcctcatat
     8641 tcacgaggtc gctgagagca cggtgggcta atgttgccat gggtagcata tactacccaa
     8701 atatctggat agcatatgct atcctaatct atatctgggt agcataggct atcctaatct
     8761 atatctgggt agcatatgct atcctaatct atatctgggt agtatatgct atcctaattt
     8821 atatctgggt agcataggct atcctaatct atatctgggt agcatatgct atcctaatct
     8881 atatctgggt agtatatgct atcctaatct gtatccgggt agcatatgct atcctaatag
     8941 agattagggt agtatatgct atcctaattt atatctgggt agcatatact acccaaatat
     9001 ctggatagca tatgctatcc taatctatat ctgggtagca tatgctatcc taatctatat
     9061 ctgggtagca taggctatcc taatctatat ctgggtagca tatgctatcc taatctatat
     9121 ctgggtagta tatgctatcc taatttatat ctgggtagca taggctatcc taatctatat
     9181 ctgggtagca tatgctatcc taatctatat ctgggtagta tatgctatcc taatctgtat
     9241 ccgggtagca tatgctatcc tcatgcatat acagtcagca tatgataccc agtagtagag
     9301 tgggagtgct atcctttgca tatgccgcca cctcccaagg gggcgtgaat tttcgctgct
     9361 tgtccttttc ctgctggttg ctcccattct taggtgaatt taaggaggcc aggctaaagc
     9421 cgtcgcatgt ctgattgctc accaggtaaa tgtcgctaat gttttccaac gcgagaaggt
     9481 gttgagcgcg gagctgagtg acgtgacaac atgggtatgc ccaattgccc catgttggga
     9541 ggacgaaaat ggtgacaaga cagatggcca gaaatacacc aacagcacgc atgatgtcta
     9601 ctggggattt attctttagt gcgggggaat acacggcttt taatacgatt gagggcgtct
     9661 cctaacaagt
//
