LOCUS       sequence-270607-        4413 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  codon optimized expression and purification of human FANCD2 in 
            insect cells using baculovirus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4413)
  AUTHORS   Tan W, Murphy VJ, Charron A, van Twest S, Sharp M, Constantinou A, 
            Parker MW, Crismani W, Bythell-Douglas R, Deans AJ
  TITLE     Preparation and purification of mono-ubiquitinated proteins using 
            Avi-tagged ubiquitin.
  JOURNAL   PLoS One. 2020 Feb 24;15(2):e0229000. doi: 
            10.1371/journal.pone.0229000. eCollection 2020.
  PUBMED    32092106
REFERENCE   2  (bases 1 to 4413)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2020-02-24"; volume: "15(2):e0229000. doi"; pages: " 
            10.1371/journal.pone.0229000. eCollection 2020"
COMMENT     Sequence Label: pFastbac1-Flag-FANCD2
FEATURES             Location/Qualifiers
     source          1..4413
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             22..45
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gagctcctag ctagctagat ggactacaaa gacgatgacg acaagggagc agtgtctaaa
       61 cgccgcctct caaaatcaga agataaagaa agcctcaccg aagatgcctc aaaaacccgt
      121 aaacagccac tctcaaagaa gaccaagaag agccacatcg ccaacgaggt ggaggaaaac
      181 gactctatct tcgtgaagct gctgaagatc agcggaatca tcctgaagac cggtgaatcc
      241 cagaaccagc tggccgtgga ccagatcgct ttccagaaga agctgttcca gaccctgcgc
      301 aggcacccta gctacccaaa gatcatcgag gagttcgtga gcggcctgga gtcttacatc
      361 gaggacgaag actctttcag aaactgcctg ctgagctgcg aacgcctgca ggacgaggaa
      421 gcctccatgg gagccagcta ctccaagtct ctgatcaagc tgctgctggg tatcgacatc
      481 ctgcagcccg ctatcatcaa gaccctgttc gaaaagctgc ctgagtactt cttcgaaaac
      541 aagaactctg acgagatcaa catcccccgc ctgatcgtga gccagctgaa gtggctggac
      601 agggtggtgg acggcaagga cctgaccacc aagatcatgc agctgatctc catcgcccca
      661 gagaacctgc agcacgacat catcaccagc ctgcccgaaa tcctgggaga ctctcagcac
      721 gctgacgtgg gcaaggagct gagcgacctg ctgatcgaaa acaccagcct gaccgtgcct
      781 atcctggacg tgctgagcag cctgcgcctg gacccaaact tcctgctgaa ggtgaggcag
      841 ctggtcatgg acaagctgtc tagcatccgc ctggaggacc tgcctgtgat catcaagttc
      901 atcctgcact ccgtgaccgc catggacacc ctggaagtga tctctgagct gagagaaaag
      961 ctggacctgc agcactgcgt gctgccatcc cgcctgcagg cctctcaggt gaagctgaag
     1021 agcaagggca gggcttcctc tagcggaaac caggagtcct ctggtcagtc ctgcatcatc
     1081 ctgctgttcg acgtgatcaa gtctgccatc cgctacgaga agaccatcag cgaagcctgg
     1141 atcaaggcta tcgagaacac cgccagcgtg agcgaacaca aggtgttcga cctggtcatg
     1201 ctgttcatca tctactccac caacacccag accaagaagt acatcgacag ggtgctgaga
     1261 aacaagatcc gctctggctg catccaggag cagctgctgc agtccacctt ctctgtgcac
     1321 tacctggtgc tgaaggacat gtgcagctcc atcctgagcc tggcccagtc cctgctgcac
     1381 tctctggacc agagcatcat ctccttcgga tctctgctgt acaagtacgc cttcaagttc
     1441 ttcgacacct actgccagca ggaagtggtg ggtgctctgg tgacccacat ctgcagcggc
     1501 aacgaggccg aagtggacac cgctctggac gtgctgctgg aactggtggt gctgaaccca
     1561 tctgccatga tgatgaacgc cgtgttcgtg aagggtatcc tggactacct ggacaacatc
     1621 agcccccagc agatccgcaa gctgttctac gtgctgagca ccctggcctt ctccaagcag
     1681 aacgaggctt ctagccacat ccaggacgac atgcacctgg tcatccgcaa gcagctgtcc
     1741 tctaccgtgt tcaagtacaa gctgatcggc atcatcggag ccgtgaccat ggctggcatc
     1801 atggccgctg acagatctga gagcccctcc ctgacccagg aacgcgccaa cctgtccgac
     1861 gagcagtgca cccaggtgac ctctctgctg cagctggtgc actcttgttc cgagcagagc
     1921 ccccaggcct ccgctctgta ctacgacgag ttcgccaacc tgatccagca cgagaagctg
     1981 gaccctaagg ctctggaatg ggtgggccac accatctgca acgacttcca ggacgccttc
     2041 gtggtggaca gctgcgtggt gcctgaggga gacttcccct tccctgtgaa ggctctgtac
     2101 ggactggagg aatacgacac ccaggacggt atcgccatca acctgctgcc cctgctgttc
     2161 agccaggact tcgctaagga cggcggacct gtgaccagcc aggagtccgg acagaagctg
     2221 gtgagccccc tgtgcctggc cccttacttc cgcctgctga ggctgtgcgt ggaacgccag
     2281 cacaacggta acctggagga aatcgacggc ctgctggact gccctatctt cctgaccgac
     2341 ctggagccag gtgaaaagct ggagtccatg tctgccaagg agagatcttt catgtgcagc
     2401 ctgatcttcc tgaccctgaa ctggttccgc gaaatcgtga acgctttctg ccaggagacc
     2461 agccctgaaa tgaagggaaa ggtgctgacc cgcctgaagc acatcgtgga gctgcagatc
     2521 atcctggaaa agtacctggc tgtgacccca gactacgtgc cccctctggg taacttcgac
     2581 gtggagaccc tggacatcac ccctcacacc gtgaccgcca tctctgctaa gatccgcaag
     2641 aagggcaaga tcgaaaggaa gcagaagacc gacggcagca agaccagctc ctctgacacc
     2701 ctgtctgagg aaaagaacag cgagtgtgac ccaaccccta gccacagggg acagctgaac
     2761 aaggagttca ccggcaagga agagaagacc agcctgctgc tgcacaactc ccacgccttc
     2821 ttcagggagc tggacatcga agtgttctcc atcctgcact gcggtctggt gaccaagttc
     2881 atcctggaca ccgagatgca caccgaagct accgaggtgg tgcagctggg cccacccgag
     2941 ctgctgttcc tgctggaaga cctgtcccag aagctggagt ctatgctgac ccctccaatc
     3001 gccagacgcg tgccattcct gaagaacaag ggttctagga acatcggctt cagccacctg
     3061 cagcagagat ccgctcagga gatcgtgcac tgcgtgttcc agctgctgac ccctatgtgc
     3121 aaccacctgg agaacatcca caactacttc cagtgcctgg ccgctgaaaa ccacggcgtg
     3181 gtggacggtc ctggcgtgaa ggtgcaggag taccacatca tgagcagctg ctaccagcgc
     3241 ctgctgcaga tcttccacgg cctgttcgcc tggtctggat tcagccagcc agagaaccag
     3301 aacctgctgt actccgctct gcacgtgctg tctagccgcc tgaagcaggg agaacactct
     3361 cagcctctgg aggaactgct gagccagtcc gtgcactacc tgcagaactt ccaccagtcc
     3421 atcccatctt tccagtgcgc cctgtacctg atccgcctgc tgatggtcat cctggagaag
     3481 agcaccgcct ccgctcagaa caaggaaaag atcgcctctc tggctaggca gttcctgtgc
     3541 agagtgtggc ccagcggaga caaggagaag tctaacatca gcaacgacca gctgcacgct
     3601 ctgctgtgca tctacctgga acacaccgag agcatcctga aggccatcga ggaaatcgct
     3661 ggagtgggtg tgccagagct gatcaactcc cccaaggacg cctcctctag caccttccca
     3721 accctgacca ggcacacctt cgtggtgttc ttcagagtga tgatggctga gctggaaaag
     3781 accgtgaaga agatcgagcc tggaactgct gctgactccc agcagatcca cgaggaaaag
     3841 ctgctgtact ggaacatggc cgtgcgcgac ttcagcatcc tgatcaacct gatcaaggtg
     3901 ttcgactccc accctgtgct gcacgtgtgc ctgaagtacg gtcgcctgtt cgtggaggct
     3961 ttcctgaagc agtgcatgcc actgctggac ttcagcttca gaaagcaccg cgaagacgtg
     4021 ctgtccctgc tggagacctt ccagctggac accaggctgc tgcaccacct gtgcggccac
     4081 agcaagatcc accaggacac cagactgacc cagcacgtgc ctctgctgaa gaagaccctg
     4141 gagctgctgg tgtgcagagt gaaggccatg ctgaccctga acaactgccg cgaagctttc
     4201 tggctgggca acctgaagaa cagggacctg cagggagagg aaatcaagtc ccagaactct
     4261 caggagagca ccgccgacga gtccgaagac gacatgtcct ctcaggcctc taagagcaag
     4321 gctaccgaag acggcgagga agacgaagtc tctgctggtg aaaaagaaca ggacagtgac
     4381 gaaagttatg atgacagtga ttgaccgctc gag
//
