LOCUS       sequence-270628-         643 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Used to sense miRNA activity using fluorescence based tools. 
            hsa-miR-200b-5p target in 3' UTR of of mKate. miRNA activity can be 
            measured by the repression of mKate2 relative to EBFP2 fluorescent 
            proteins . Plasmid is inherently unstable; please see Depositor 
            Comments.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 643)
  AUTHORS   Gam JJ, Babb J, Weiss R
  TITLE     A mixed antagonistic/synergistic miRNA repression model enables 
            accurate predictions of multi-input miRNA sensor activity.
  JOURNAL   Nat Commun. 2018 Jun 22;9(1):2430. doi: 10.1038/s41467-018-04575-0.
  PUBMED    29934631
REFERENCE   2  (bases 1 to 643)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-06-22"; volume: "9(1):2430. doi"; pages: " 
            10.1038/s41467-018-04575-0"
COMMENT     Sequence Label: LSB-hsa-miR-200b-5p
FEATURES             Location/Qualifiers
     source          1..643
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(114..138)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(362..381)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    427..482
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(481..500)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
ORIGIN
        1 aagaatcaag gaggccgaca aagagacata cgtcgagcag cacgaggtgg ctgtggccag
       61 atactgcgac ctccctagca aactggggca caaacttaat tgagaattct accacccagc
      121 tttcttgtac aaagtggtac gtctcaagac cagaggtctc tgcttttcat aagtccaatg
      181 ctgcccagta agatgtccaa tgctgcccag taagatgtcc aatgctgccc agtaagatgt
      241 ccaatgctgc ccagtaagat gcaaccagaa gagacctgtg cttcactcct caggtgcagg
      301 ctgcctatca gaaggtggtg gctggtgtgg ccaatgccct ggctcacaaa taccactgag
      361 atctttttcc ctctgccaaa aattatgggg acatcatgaa gccccttgag catctgactt
      421 ctggctaata aaggaaattt attttcattg caatagtgtg ttggaatttt ttgtgtctct
      481 cactcggaag gacatatggg agggcaaatc atttaaaaca tcagaatgag tatttggttt
      541 agagtttggc aacatatgcc catatgctgg ctgccatgaa caaaggttgg ctataaagag
      601 gtcatcagta tatgaaacag ccccctgctg tccattcctt att
//
