LOCUS       sequence-270649-        8816 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses an inducible short hairpin targeting human NRF2 sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8816)
  AUTHORS   Pereira EJ, Burns JS, Lee CY, Marohl T, Calderon D, Wang L, Atkins 
            KA, Wang CC, Janes KA
  TITLE     Sporadic activation of an oxidative stress-dependent NRF2-p53 
            signaling network in breast epithelial spheroids and 
            premalignancies.
  JOURNAL   Sci Signal. 2020 Apr 14;13(627). pii: 13/627/eaba4200. doi: 
            10.1126/scisignal.aba4200.
  PUBMED    32291314
REFERENCE   2  (bases 1 to 8816)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2020-04-14"; volume: "13(627). pii: 
            13/627/eaba4200. doi"; pages: " 10.1126/scisignal.aba4200"
COMMENT     Sequence Label: tet_pLKO.1_puro_shNRF2 #1
FEATURES             Location/Qualifiers
     source          1..8816
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..234
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             419..463
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        752..846
                     /label=H1-2O2 promoter
                     /note="doxycycline-inducible variant of the human H1 RNA 
                     promoter (Henriksen et al., 2007)"
     primer_bind     763..782
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     protein_bind    800..818
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    828..846
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     misc_feature    947..1064
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1113..1623
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     1542..1560
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     intron          1632..2204
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     2212..2231
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     promoter        2259..2277
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2288..2908
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(2361..2380)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     misc_feature    2943..3516
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3110..3127)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3337..3356
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3536..4135
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3536..3555)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4032..4052
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             4263..4496
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4568..4689
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4605..4624)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4659..4678
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4729..4864
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(4885..4903)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4913..4930)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4913..4929)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4922..4944)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5071..5526
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5158..5177)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5368..5389
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5552..5656
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5657..5725,5726..6517)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5875..5894)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6688..7276
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7177..7196
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7430..7447
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7564..7585
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7600..7605,7606..7623,7624..7630)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7638..7654
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7643..7665
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7662..7678
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7662..7678
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7697..7717
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7699..7717
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7745..7971
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7972..8152
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8199..8324
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
ORIGIN
        1 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat
       61 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt
      121 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca
      181 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat
      241 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag
      301 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat
      361 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa
      421 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat
      481 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt
      541 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt
      601 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga
      661 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta
      721 tcgatcacga gactagcctc gagcggccgc aatatttgca tgtcgctatg tgttctggga
      781 aatcaccata aacgtgaaat ccctatcagt gatagagact tataagttcc ctatcagtga
      841 tagagacacc ggagagcaag atttagatca tttctgcaga aatgatctaa atcttgctct
      901 tttttgaatt ctcgacctcg agacaaatgg cagtattcat ccacaatttt aaaagaaaag
      961 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     1021 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     1081 acagcagaga tccactttgg ccgcggctcg agggggttgg ggttgcgcct tttccaaggc
     1141 agccctgggt ttgcgcaggg acgcggctgc tctgggcgtg gttccgggaa acgcagcggc
     1201 gccgaccctg ggtctcgcac attcttcacg tccgttcgca gcgtcacccg gatcttcgcc
     1261 gctacccttg tgggcccccc ggcgacgctt cctgctccgc ccctaagtcg ggaaggttcc
     1321 ttgcggttcg cggcgtgccg gacgtgacaa acggaagccg cacgtctcac tagtaccctc
     1381 gcagacggac agcgccaggg agcaatggca gcgcgccgac cgcgatgggc tgtggccaat
     1441 agcggctgct cagcagggcg cgccgagagc agcggccggg aaggggcggt gcgggaggcg
     1501 gggtgtgggg cggtagtgtg ggccctgttc ctgcccgcgc ggtgttccgc attctgcaag
     1561 cctccggagc gcacgtcggc agtcggctcc ctcgttgacc gaatcaccga cctctctccc
     1621 cagggggatc tgtgagtttg gggacccttg attgttcttt ctttttcgct attgtaaaat
     1681 tcatgttata tggagggggc aaagttttca gggtgttgtt tagaatggga agatgtccct
     1741 tgtatcacca tggaccctca tgataatttt gtttctttca ctttctactc tgttgacaac
     1801 cattgtctcc tcttattttc ttttcatttt ctgtaacttt ttcgttaaac tttagcttgc
     1861 atttgtaacg aatttttaaa ttcacttttg tttatttgtc agattgtaag tactttctct
     1921 aatcactttt ttttcaaggc aatcagggta tattatattg tacttcagca cagttttaga
     1981 gaacaattgt tataattaaa tgataaggta gaatatttct gcatataaat tctggctggc
     2041 gtggaaatat tcttattggt agaaacaact acatcctggt catcatcctg cctttctctt
     2101 tatggttaca atgatataca ctgtttgaga tgaggataaa atactctgag tccaaaccgg
     2161 gcccctctgc taaccatgtt catgccttct tctttttcct acagctcctg ggcaacgtgc
     2221 tggttattgt gctgtctcat cattttggca aagaattgta atacgactca ctatagggcg
     2281 aattgatatg tctagattag ataaaagtaa agtgattaac agcgcattag agctgcttaa
     2341 tgaggtcgga atcgaaggtt taacaacccg taaactcgcc cagaagctag gtgtagagca
     2401 gcctacattg tattggcatg taaaaaataa gcgggctttg ctcgacgcct tagccattga
     2461 gatgttagat aggcaccata ctcacttttg ccctttagaa ggggaaagct ggcaagattt
     2521 tttacgtaat aacgctaaaa gttttagatg tgctttacta agtcatcgcg atggagcaaa
     2581 agtacattta ggtacacggc ctacagaaaa acagtatgaa actctcgaaa atcaattagc
     2641 ctttttatgc caacaaggtt tttcactaga gaatgcatta tatgcactca gcgctgtggg
     2701 gcattttact ttaggttgcg tattggaaga tcaagagcat caagtcgcta aagaagaaag
     2761 ggaaacacct actactgata gtatgccgcc attattacga caagctatcg aattatttga
     2821 tcaccaaggt gcagagccag ccttcttatt cggccttgaa ttgatcatat gcggattaga
     2881 aaaacaactt aaatgtgaaa gtgggtccgc gtacagcggc tcccgggagt tctagggatc
     2941 tgcccctctc cctccccccc ccctaacgtt actggccgaa gccgcttgga ataaggccgg
     3001 tgtgcgtttg tctatatgtt attttccacc atattgccgt cttttggcaa tgtgagggcc
     3061 cggaaacctg gccctgtctt cttgacgagc attcctaggg gtctttcccc tctcgccaaa
     3121 ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga
     3181 caaacaacgt ctgtagcgac cctttgcagg cagcggaacc ccccacctgg cgacaggtgc
     3241 ctctgcggcc aaaagccacg tgtataagat acacctgcaa aggcggcaca accccagtgc
     3301 cacgttgtga gttggatagt tgtggaaaga gtcaaatggc tctcctcaag cgtattcaac
     3361 aaggggctga aggatgccca gaaggtaccc cattgtatgg gatctgatct ggggcctcgg
     3421 tgcacatgct ttacatgtgt ttagtcgagg ttaaaaaaac gtctaggccc cccgaaccac
     3481 ggggacgtgg ttttcctttg aaaaacacga tgataaggat ccaccggagc ttaccatgac
     3541 cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac
     3601 cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca
     3661 catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg
     3721 caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt
     3781 cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg
     3841 gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc
     3901 gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc
     3961 cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac
     4021 ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt
     4081 cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgacgccc
     4141 gccccacgac ccgcagcgcc cgaccgaaag gagcgcacga ccccatgcat cggtaccttt
     4201 aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg
     4261 actggaaggg ctaattcact cccaacgaag acaagatctg ctttttgctt gtactgggtc
     4321 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     4381 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     4441 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag
     4501 tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag
     4561 tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     4621 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     4681 tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccat cccgccccta
     4741 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     4801 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     4861 ggcctaggga cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc tcactggccg
     4921 tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag
     4981 cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc
     5041 aacagttgcg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg
     5101 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
     5161 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     5221 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     5281 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
     5341 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
     5401 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
     5461 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta
     5521 caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     5581 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     5641 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     5701 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     5761 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     5821 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     5881 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     5941 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     6001 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     6061 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     6121 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     6181 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
     6241 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     6301 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     6361 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     6421 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     6481 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     6541 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     6601 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     6661 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     6721 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     6781 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     6841 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     6901 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     6961 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     7021 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     7081 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     7141 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     7201 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     7261 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     7321 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     7381 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     7441 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
     7501 tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc
     7561 aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc
     7621 tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca
     7681 tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg agctgcaagc
     7741 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
     7801 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
     7861 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
     7921 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
     7981 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     8041 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     8101 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
     8161 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
     8221 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
     8281 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
     8341 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
     8401 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
     8461 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
     8521 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
     8581 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
     8641 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
     8701 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     8761 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaat
//
