LOCUS       sequence-270657-        8816 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses an inducible short hairpin targeting firefly luciferase 
            sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8816)
  AUTHORS   Pereira EJ, Burns JS, Lee CY, Marohl T, Calderon D, Wang L, Atkins 
            KA, Wang CC, Janes KA
  TITLE     Sporadic activation of an oxidative stress-dependent NRF2-p53 
            signaling network in breast epithelial spheroids and 
            premalignancies.
  JOURNAL   Sci Signal. 2020 Apr 14;13(627). pii: 13/627/eaba4200. doi: 
            10.1126/scisignal.aba4200.
  PUBMED    32291314
REFERENCE   2  (bases 1 to 8816)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2020-04-14"; volume: "13(627). pii: 
            13/627/eaba4200. doi"; pages: " 10.1126/scisignal.aba4200"
COMMENT     Sequence Label: tet_pLKO.1_puro_shLuc
FEATURES             Location/Qualifiers
     source          1..8816
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    178..751
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(345..362)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     572..591
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             771..1370
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(771..790)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     1267..1287
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             1498..1731
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    1803..1924
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1840..1859)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1894..1913
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      1964..2099
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(2120..2138)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2148..2165)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2148..2164)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2157..2179)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      2306..2761
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2393..2412)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2603..2624
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2787..2891
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2892..2960,2961..3752)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3110..3129)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3923..4511
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4412..4431
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4665..4682
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4799..4820
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4835..4840,4841..4858,4859..4865)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4873..4889
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4878..4900
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4897..4913
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4897..4913
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     4932..4952
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        4934..4952
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        4980..5206
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             5207..5387
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    5434..5559
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6052..6285
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6470..6514
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        6803..6897
                     /label=H1-2O2 promoter
                     /note="doxycycline-inducible variant of the human H1 RNA 
                     promoter (Henriksen et al., 2007)"
     primer_bind     6814..6833
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     protein_bind    6851..6869
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6879..6897
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     misc_feature    6998..7115
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        7164..7674
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     7593..7611
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     intron          7683..8255
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     8263..8282
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     promoter        8310..8328
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(8339..8816,1..143)
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(8412..8431)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
ORIGIN
        1 cacctactac tgatagtatg ccgccattat tacgacaagc tatcgaatta tttgatcacc
       61 aaggtgcaga gccagccttc ttattcggcc ttgaattgat catatgcgga ttagaaaaac
      121 aacttaaatg tgaaagtggg tccgcgtaca gcggctcccg ggagttctag ggatctgccc
      181 ctctccctcc ccccccccta acgttactgg ccgaagccgc ttggaataag gccggtgtgc
      241 gtttgtctat atgttatttt ccaccatatt gccgtctttt ggcaatgtga gggcccggaa
      301 acctggccct gtcttcttga cgagcattcc taggggtctt tcccctctcg ccaaaggaat
      361 gcaaggtctg ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt gaagacaaac
      421 aacgtctgta gcgacccttt gcaggcagcg gaacccccca cctggcgaca ggtgcctctg
      481 cggccaaaag ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc agtgccacgt
      541 tgtgagttgg atagttgtgg aaagagtcaa atggctctcc tcaagcgtat tcaacaaggg
      601 gctgaaggat gcccagaagg taccccattg tatgggatct gatctggggc ctcggtgcac
      661 atgctttaca tgtgtttagt cgaggttaaa aaaacgtcta ggccccccga accacgggga
      721 cgtggttttc ctttgaaaaa cacgatgata aggatccacc ggagcttacc atgaccgagt
      781 acaagcccac ggtgcgcctc gccacccgcg acgacgtccc cagggccgta cgcaccctcg
      841 ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgatccggac cgccacatcg
      901 agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg
      961 tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag
     1021 cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg
     1081 ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt
     1141 tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg
     1201 tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg
     1261 cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg
     1321 tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctga cgcccgcccc
     1381 acgacccgca gcgcccgacc gaaaggagcg cacgacccca tgcatcggta cctttaagac
     1441 caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg
     1501 aagggctaat tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct
     1561 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     1621 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     1681 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt
     1741 catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga
     1801 ggaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     1861 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     1921 cttatcatgt ctggctctag ctatcccgcc cctaactccg cccatcccgc ccctaactcc
     1981 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     2041 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
     2101 agggacgtac ccaattcgcc ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt
     2161 ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat
     2221 ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
     2281 ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt
     2341 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
     2401 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     2461 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     2521 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     2581 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
     2641 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
     2701 aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt
     2761 taggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     2821 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     2881 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     2941 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     3001 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     3061 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     3121 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     3181 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     3241 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     3301 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     3361 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     3421 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     3481 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     3541 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     3601 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     3661 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     3721 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     3781 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     3841 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     3901 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     3961 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     4021 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     4081 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     4141 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     4201 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     4261 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     4321 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     4381 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     4441 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     4501 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     4561 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     4621 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     4681 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     4741 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     4801 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     4861 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
     4921 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat
     4981 gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc
     5041 cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg
     5101 tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc
     5161 gcattgcaga gatattgtat ttaagtgcct agctcgatac ataaacgggt ctctctggtt
     5221 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     5281 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     5341 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac
     5401 agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct
     5461 gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact
     5521 agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt
     5581 agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa
     5641 catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa
     5701 acatcagaag gctgtagaca aatactggga cagctacaac catcccttca gacaggatca
     5761 gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata
     5821 gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag
     5881 accaccgcac agcaagcggc cgctgatctt cagacctgga ggaggagata tgagggacaa
     5941 ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc
     6001 caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt
     6061 gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac
     6121 ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc
     6181 tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc
     6241 aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg
     6301 ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc
     6361 tctggaacag atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta
     6421 cacaagctta atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca
     6481 agaattattg gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg
     6541 gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt
     6601 ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca
     6661 gacccacctc ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg
     6721 agagagagac agagacagat ccattcgatt agtgaacgga tctcgacggt atcgatcacg
     6781 agactagcct cgagcggccg caatatttgc atgtcgctat gtgttctggg aaatcaccat
     6841 aaacgtgaaa tccctatcag tgatagagac ttataagttc cctatcagtg atagagacac
     6901 cggagaatcg tcgtatgcag tgaactgcag ttcactgcat acgacgattc ttttttgaat
     6961 tctcgacctc gagacaaatg gcagtattca tccacaattt taaaagaaaa ggggggattg
     7021 gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag
     7081 aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag
     7141 atccactttg gccgcggctc gagggggttg gggttgcgcc ttttccaagg cagccctggg
     7201 tttgcgcagg gacgcggctg ctctgggcgt ggttccggga aacgcagcgg cgccgaccct
     7261 gggtctcgca cattcttcac gtccgttcgc agcgtcaccc ggatcttcgc cgctaccctt
     7321 gtgggccccc cggcgacgct tcctgctccg cccctaagtc gggaaggttc cttgcggttc
     7381 gcggcgtgcc ggacgtgaca aacggaagcc gcacgtctca ctagtaccct cgcagacgga
     7441 cagcgccagg gagcaatggc agcgcgccga ccgcgatggg ctgtggccaa tagcggctgc
     7501 tcagcagggc gcgccgagag cagcggccgg gaaggggcgg tgcgggaggc ggggtgtggg
     7561 gcggtagtgt gggccctgtt cctgcccgcg cggtgttccg cattctgcaa gcctccggag
     7621 cgcacgtcgg cagtcggctc cctcgttgac cgaatcaccg acctctctcc ccagggggat
     7681 ctgtgagttt ggggaccctt gattgttctt tctttttcgc tattgtaaaa ttcatgttat
     7741 atggaggggg caaagttttc agggtgttgt ttagaatggg aagatgtccc ttgtatcacc
     7801 atggaccctc atgataattt tgtttctttc actttctact ctgttgacaa ccattgtctc
     7861 ctcttatttt cttttcattt tctgtaactt tttcgttaaa ctttagcttg catttgtaac
     7921 gaatttttaa attcactttt gtttatttgt cagattgtaa gtactttctc taatcacttt
     7981 tttttcaagg caatcagggt atattatatt gtacttcagc acagttttag agaacaattg
     8041 ttataattaa atgataaggt agaatatttc tgcatataaa ttctggctgg cgtggaaata
     8101 ttcttattgg tagaaacaac tacatcctgg tcatcatcct gcctttctct ttatggttac
     8161 aatgatatac actgtttgag atgaggataa aatactctga gtccaaaccg ggcccctctg
     8221 ctaaccatgt tcatgccttc ttctttttcc tacagctcct gggcaacgtg ctggttattg
     8281 tgctgtctca tcattttggc aaagaattgt aatacgactc actatagggc gaattgatat
     8341 gtctagatta gataaaagta aagtgattaa cagcgcatta gagctgctta atgaggtcgg
     8401 aatcgaaggt ttaacaaccc gtaaactcgc ccagaagcta ggtgtagagc agcctacatt
     8461 gtattggcat gtaaaaaata agcgggcttt gctcgacgcc ttagccattg agatgttaga
     8521 taggcaccat actcactttt gccctttaga aggggaaagc tggcaagatt ttttacgtaa
     8581 taacgctaaa agttttagat gtgctttact aagtcatcgc gatggagcaa aagtacattt
     8641 aggtacacgg cctacagaaa aacagtatga aactctcgaa aatcaattag cctttttatg
     8701 ccaacaaggt ttttcactag agaatgcatt atatgcactc agcgctgtgg ggcattttac
     8761 tttaggttgc gtattggaag atcaagagca tcaagtcgct aaagaagaaa gggaaa
//
