LOCUS       sequence-270698-       10767 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Control for optogenetic machinery 
            (pSTRONG-VP16-CIB1/pMEDIUM-ZCRY2PHR) integration with pWEAK-mRUBY2 
            reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10767)
  AUTHORS   An-Adirekkun JM, Stewart CJ, Geller SH, Patel MT, Melendez J, Oakes 
            BL, Noyes MB, McClean MN
  TITLE     A yeast optogenetic toolkit (yOTK) for gene expression control in 
            Saccharomyces cerevisiae.
  JOURNAL   Biotechnol Bioeng. 2019 Dec 2. doi: 10.1002/bit.27234.
  PUBMED    31788779
REFERENCE   2  (bases 1 to 10767)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2019 Dec 2. doi: 10.1002/bit.27234."
COMMENT     Sequence Label: pMM0649
FEATURES             Location/Qualifiers
     source          1..10767
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     229..248
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        229..247
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             272..781
                     /codon_start=1
                     /product="N-terminal portion of the CIB1 transcription 
                     factor from Arabidopsis thaliana"
                     /label=CIBN
                     /note="binds to CRY2 (cryptochrome 2) that has absorbed 
                     blue light (Kennedy et al., 2010)"
                     /translation="MNGAIGGDLLLNFPDMSVLERQRAHLKYLNPTFDSPLAGFFADSS
                     MITGGEMDSYLSTAGLNLPMMYGETTVEGDSRLSISPETTLGTGNFKKRKFDTETKDCN
                     EKKKKMTMNRDDLVEEGEEEKSKITEQNNGSTKSIKKMKHKAKKEENNFSNDSSKVTKE
                     LEKTDYI"
     CDS             2301..2321
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2604..4097
                     /codon_start=1
                     /product="N-terminal photolyase homology region of 
                     Arabidopsis thaliana cryptochrome 2"
                     /label=CRY2PHR
                     /note="binds to CIB1 upon exposure to blue light (Kennedy 
                     et al., 2010)"
                     /translation="MKMDKKTIVWFRRDLRIEDNPALAAAAHEGSVFPVFIWCPEEEGQ
                     FYPGRASRWWMKQSLAHLSQSLKALGSDLTLIKTHNTISAILDCIRVTGATKVVFNHLY
                     DPVSLVRDHTVKEKLVERGISVQSYNGDLLYEPWEIYCEKGKPFTSFNSYWKKCLDMSI
                     ESVMLPPPWRLMPITAAAEAIWACSIEELGLENEAEKPSNALLTRAWSPGWSNADKLLN
                     EFIEKQLIDYAKNSKKVVGNSTSLLSPYLHFGEISVRHVFQCARMKQIIWARDKNSEGE
                     ESADLFLRGIGLREYSRYICFNFPFTHEQSLLSHLRFFPWDADVDKFKAWRQGRTGYPL
                     VDAGMRELWATGWMHNRIRVIVSSFAVKFLLLPWKWGMKYFWDTLLDADLECDILGWQY
                     ISGSIPDGHELDRLDNPALQGAKYDPEGEYIRQWLPELARLPTEWIHHPWDAPLTVLKA
                     SGVELGTNYAKPIVDIDTARELLAKAISRTREAQIMIGAA"
     CDS             5083..5793
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby, with improved photophysical properties (Lam et al., 
                     2012)"
                     /label=mRuby2
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTH
                     MALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAV
                     AKFAGLGGGMDELYK"
     terminator      5809..5996
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(5864..5888)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     CDS             6259..7062
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     CDS             complement(7704..8519)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(7704..8519)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     8427..8446
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     8511..8530
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      8744..9332
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9233..9252
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        10285..10698
                     /gene="S. cerevisiae TEF1"
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     CDS             join(10748..10767,1)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 cgaattgggt accgccgcct cggagctcca cttagacggc gaggacgtgg cgatggcgca
       61 tgccgacgcg ctagacgatt tcgatctgga catgttgggg gacggggatt ccccgggtcc
      121 gggatttacc ccccacgact ccgcccccta cggcgctctg gatatggccg acttcgagtt
      181 tgagcagatg tttaccgatg cccttggaat tgacgagtac ggtgggttta atacgactca
      241 ctatagggcg agcgccgaag ctagcgccac catgaatgga gctataggag gtgacctttt
      301 gctcaatttt cctgacatgt cggtcctaga gcgccaaagg gctcacctca agtacctcaa
      361 tcccaccttt gattctcctc tcgccggctt ctttgccgat tcttcaatga ttaccggcgg
      421 cgagatggac agctatcttt cgactgccgg tttgaatctt ccgatgatgt acggtgaaac
      481 gacggtggaa ggtgattcaa gactctcaat ttcgccggaa acgacgcttg ggactggaaa
      541 tttcaagaaa cggaagtttg atacagagac taaggattgt aatgagaaga agaagaagat
      601 gacgatgaac agagatgacc tagtagaaga aggagaagaa gagaagtcga aaataacaga
      661 gcaaaacaat gggagcacaa aaagcatcaa gaagatgaaa cacaaagcca agaaagaaga
      721 gaacaatttc tctaatgatt catctaaagt gacgaaggaa ttggagaaaa cggattatat
      781 tcatgttcgt gcacgacgag gccaagccac tgatagtcac agcatagcag aacgagttag
      841 aagagaaaag atcagtgaga gaatgaagtt tctacaagat ttggttcctg gatgcgacaa
      901 gatcacaggc aaagcaggga tgcttgatga aatcattaac tatgttcagt ctcttcagag
      961 acaaatcgag ttcttatcga tgaaactagc aattgtgaat ccaaggccgg attttgatat
     1021 ggatgacatt tttgccaaag aggttgcctc aactccaatg actgtggtgc catctcctga
     1081 aatggttctt tccggttatt ctcatgagat ggttcactct ggttattcta gtgagatggt
     1141 taactccggt taccttcatg tcaatccaat gcagcaagtg aataccagtt ctgatccatt
     1201 gtcatgcttc aacaatggcg aagctccttc gatgtgggac tctcatgtgc agaatctcta
     1261 tggcaattta ggagtaccgg tcatcgagct cgagctgcag atgaatcgta gatacggatc
     1321 ctaactcgag agcttttgat taagccttct agtccaaaaa acacgttttt ttgtcattta
     1381 tttcattttc ttagaatagt ttagtttatt cattttatag tcacgaatgt tttatgattc
     1441 tatatagggt tgcaaacaag catttttcat tttatgttaa aacaatttca ggtttacctt
     1501 ttattctgct tgtggtgacg cgtgtatccg cccgctcttt tggtcaccca tgtatgctgc
     1561 caaaccagat gtcaacacag ctacaacgaa gaggatgtcc aatatttttt ttaaggaata
     1621 aggatacttc aagactagat tcccccctgc attcccatca gaaccgtaaa ccttggcgct
     1681 ttccttggga agtattcaag aagtgccttg tccggtttct gtggctcaca aaccagcgcg
     1741 cccgatatgg ctttcttttc acttatgaat gtaccagtac gggacaatta gaacgctcct
     1801 gtaacaatct ctttgcaaat gtggggttac attctaacca tgtcacactg ctgacgaaat
     1861 tcaaagtaaa aaaaaatggg accacgtctt gagaacgata gattttcttt attttacatt
     1921 gaacagtcgt tgtctcagcg cgctttatgt tttcattcat acttcatatt ataaaataac
     1981 aaaagaagaa tttcatattc acgcccaaga aatcaggctg ctttccaaat gcaattgaca
     2041 cttcattagc catcacacaa aactctttct tgctggagct tcttttaaaa aagacctcag
     2101 tacaccaaac acgttacccg acctcgttat tttacgacaa ctatgataaa attctgaaga
     2161 aaaaataaaa aaattttcat acttcttgct tttatttaaa ccattgaatg atttcttttg
     2221 aacaaaacta cctgtttcac caaaggaaat agaaagaaaa aatcaattag aagaaaacaa
     2281 aaaacaaaag atctatggcc cccaagaaga agaggaaggt tggtattcac gggggtaccc
     2341 gcccatatgc ttgccctgtc gagtcctgcg atcgccgctt ttctcgctcg gatgagctta
     2401 cccgccatat ccgcatccat accggtcaga agcccttcca gtgtcgaatc tgcatgcgta
     2461 acttcagtcg tagtgaccac cttaccaccc acatccgcac ccacacaggc gagaagcctt
     2521 ttgcctgtga catttgtggg aggaagtttg ccaggagtga tgaacgcaag aggcatacca
     2581 aaatccatac aggtgctagc ttcatgaaga tggacaaaaa gactatagtt tggtttagaa
     2641 gagatctaag gattgaggat aatcctgcat tagcagcagc tgctcacgaa ggatctgttt
     2701 ttcctgtctt catttggtgt cctgaagaag aaggacagtt ttatcctgga agagcttcaa
     2761 gatggtggat gaaacaatca cttgctcact tatctcaatc cttgaaggct cttggatctg
     2821 acctcacttt aatcaaaacc cacaacacga tttcagcgat cttggattgt atccgcgtta
     2881 ccggtgctac aaaagtcgtc tttaaccacc tctatgatcc tgtttcgtta gttcgggacc
     2941 ataccgtaaa ggagaagctg gtggaacgtg ggatctctgt gcaaagctac aatggagatc
     3001 tattgtatga accgtgggag atatactgcg aaaagggcaa accttttacg agtttcaatt
     3061 cttactggaa gaaatgctta gatatgtcga ttgaatccgt tatgcttcct cctccttggc
     3121 ggttgatgcc aataactgca gcggctgaag cgatttgggc gtgttcgatt gaagaactag
     3181 ggctggagaa tgaggccgag aaaccgagca atgcgttgtt aactagagct tggagtccag
     3241 gatggagcaa tgctgataag ttactaaatg agttcatcga gaagcagttg atagattatg
     3301 caaagaacag caagaaagtt gttgggaatt ctacttcact actttctccg tatctccatt
     3361 tcggggaaat aagcgtcaga cacgttttcc agtgtgcccg gatgaaacaa attatatggg
     3421 caagagataa gaacagtgaa ggagaagaaa gtgcagatct ttttcttagg ggaatcggtt
     3481 taagagagta ttctcggtat atatgtttca acttcccgtt tactcacgag caatcgttgt
     3541 tgagtcatct tcggtttttc ccttgggatg ctgatgttga taagttcaag gcctggagac
     3601 aaggcaggac cggttatccg ttggtggatg ccggaatgag agagctttgg gctaccggat
     3661 ggatgcataa cagaataaga gtgattgttt caagctttgc tgtgaagttt cttctccttc
     3721 catggaaatg gggaatgaag tatttctggg atacactttt ggatgctgat ttggaatgtg
     3781 acatccttgg ctggcagtat atctctggga gtatccccga tggccacgag cttgatcgct
     3841 tggacaatcc cgcgttacaa ggcgccaaat atgacccaga aggtgagtac ataaggcaat
     3901 ggcttcccga gcttgcgaga ttgccaactg aatggatcca tcatccatgg gacgctcctt
     3961 taaccgtact caaagcttct ggtgtggaac tcggaacaaa ctatgcgaaa cccattgtag
     4021 acatcgacac agctcgtgag ctactagcta aagctatttc aagaacccgt gaagcacaga
     4081 tcatgatcgg agcagcagcc cggggatcct aactcgaggc caattggtgc ggcaattgat
     4141 aataacgaaa atgtctttta atgatctggg tataatgagg aattttccga acgtttttac
     4201 tttatatata tatatacatg taacatatat tctatacgct atagagaaag gaaatttttc
     4261 aattaaaaaa aaaatagaga aagagtttca cttcttgatt atcgctaaca ctaatggttg
     4321 aagtactgct actttaattt tatgctggat gcacacactg gcttaagatg acaacggtgt
     4381 tgttatccga tacaaccgga tatttttctt ttaatgagtc taaaccgtga tagcttcagg
     4441 ttaatacaat caaaaaaagc tcaaatattc ttttaatgcc gcgttcacag attccaattg
     4501 aatacaacta ggtagttcat tatatgaagc ctttgctact atttttcact atagtctgcc
     4561 ttcaccttaa tgcagacatc cacatatttt aatcacttta aaataaaaag gaagatatat
     4621 tagaagctat gatccaatct gtaagccaga ttaaaattca cgaactcttc tttcatttga
     4681 attgaatgct ttgagttggg gtagattatc gcaaattact catcacattt attgactacg
     4741 aacttgctga tgtccttttt ttatttatat ttttcttcag tgaagcgatt ttttttttac
     4801 acagaccaag acggaaaaaa gtagctaagg aagaaaacaa aatcatgaaa aaaatgtgaa
     4861 gtgatcatgc acatcgcatc aacttaaaca ttggcttaga gatatataga gttagagttt
     4921 acggcaacct ttaagcacca ataccttttg gcatagtcta aagacctggt tcttaatttt
     4981 aaacaaattt aactaaagat ttccctatca aagaagtaac gagttgacag attttctcaa
     5041 aataaatcga tactgcattt ctaggcatat ccagcgagat ctatggtgtc caaaggagag
     5101 gagttaatca aggaaaacat gagaatgaaa gttgtcatgg agggctccgt taatggtcac
     5161 caattcaagt gtacagggga aggtgaaggt aatccttaca tgggtacaca aactatgaga
     5221 attaaagtaa ttgaaggcgg accactacca tttgcatttg acattctggc aacgtcattc
     5281 atgtacggat cacgaacttt catcaagtac cctaaaggta taccagactt tttcaagcaa
     5341 tcttttccag agggttttac atgggaaagg gttacaagat acgaagatgg gggtgtcgtc
     5401 acagttatgc aagatacttc attagaagat ggctgccttg tctatcatgt gcaagtaaga
     5461 ggggtgaatt ttccttctaa cggacctgtg atgcagaaaa agaccaaagg ttgggaacca
     5521 aatactgaaa tgatgtaccc agctgatgga ggtttgagag gctacacaca catggcgctt
     5581 aaagttgatg gtggaggtca tttgtcttgt agttttgtta ccacttatcg ttctaaaaag
     5641 actgttggca atatcaaaat gccaggaata catgctgtag accacagact agaaagactc
     5701 gaagagagcg ataacgaaat gttcgttgta cagagagagc atgccgtagc caaatttgct
     5761 ggcttaggcg gtggtatgga tgaattgtat aagggatcct aactcgaggc gaatttctta
     5821 tgatttatga tttttattat taaataagtt ataaaaaaaa taagtgtata caaattttaa
     5881 agtgactctt aggttttaaa acgaaaattc ttattcttga gtaactcttt cctgtaggtc
     5941 aggttgcttt ctcaggtata gcatgaggtc gctcttattg accacacctc taccggcatg
     6001 ccgagcaaat gcctgcaaat cgctccccat ttcgctgagc agttacagag atgttacgaa
     6061 ccactagtgc actgcagtac acggtttcct tgaaattttt ttgattcggt aatctccgaa
     6121 cagaaggaag aacgaaggaa ggagcacaga cttagattgg tatatatacg catatgtagt
     6181 gttgaagaaa catgaaattg cccagtattc ttaacccaac tgcacagaac aaaaacgtgc
     6241 aggaaacgaa gataaatcat gtcgaaagct acatataagg aacgtgctgc tactcatcct
     6301 agtcctgttg ctgccaagct atttaatatc atgcacgaaa agcaaacaaa cttgtgtgct
     6361 tcattggatg ttcgtaccac caaggaatta ctggagttag ttgaagcatt aggtcccaaa
     6421 atttgtttac taaaaacaca tgtggatatc ttgactgatt tttccatgga gggcacagtt
     6481 aagccgctaa aggcattatc cgccaagtac aattttttac tcttcgagga cagaaaattt
     6541 gctgacattg gtaatacagt caaattgcag tactctgcgg gtgtatacag aatagcagaa
     6601 tgggcagaca ttacgaatgc acacggtgtg gtgggcccag gtattgttag cggtttgaag
     6661 caggcggcag aagaagtaac aaaggaacct agaggccttt tgatgttagc agaattgtca
     6721 tgcaagggct ccctatctac tggagaatat actaagggta ctgttgacat tgcgaagagc
     6781 gacaaagatt ttgttatcgg ctttattgct caaagagaca tgggtggaag agatgaaggt
     6841 tacgattggt tgattatgac acccggtgtg ggtttagatg acaagggaga tgcattgggt
     6901 caacagtata gaaccgtgga tgatgtggtt tctacaggat ctgacattat tattgttgga
     6961 agaggactat ttgcaaaggg aagggatgct aaggtagagg gtgaacgtta cagaaaagca
     7021 ggctgggaag catatttgag aagatgcggc cagcaaaact aaaaaactgt attataagta
     7081 aatgcatgta tactaaactc acaaattaga gcttcaattt aattatatca gttattaccc
     7141 gagtagagca cttgaatcca ctgccccggg aatctcggtc gtaatgattt ctataatgac
     7201 gaaaaaaaaa aaattggaaa gaaaaagctt catggccttt ataaaaagga actatccaat
     7261 acctcgccag aaccaagtaa cagtatttta cggggcacaa atcaagaaca ataagacagg
     7321 actgtaaaga tggacgcatt gaactccaaa gaacaacaag agttccaaaa agtagtggaa
     7381 caaaagcaaa tgaaggattt catgcgtttg tactctaatc tggtagaaag atgtttcaca
     7441 gactgtgtca atgacttcac aacatcaaag ctaaccaata aggaacaaac atgcatcatg
     7501 aagtgctcag aaaagttctt gaagcatagc gaacgtgtag ggcagcgttt ccaagaacaa
     7561 aacgctgcct tgggacaagg cttgggccga taaggtgtac tggcgtatat atatctaatt
     7621 atgtatctct ggtgtagccc atttttagca tgtaaatata aagaccgagc ggccgcgtgt
     7681 tacaaccaat taaccaattc tgattagaaa aactcatcga gcatcaaatg aaactgcaat
     7741 ttattcatat caggattatc aataccatat ttttgaaaaa gccgtttctg taatgaagga
     7801 gaaaactcac cgaggcagtt ccataggatg gcaagatcct ggtatcggtc tgcgattccg
     7861 actcgtccaa catcaataca acctattaat ttcccctcgt caaaaataag gttatcaagt
     7921 gagaaatcac catgagtgac gactgaatcc ggtgagaatg gcaaaagctt atgcatttct
     7981 ttccagactt gttcaacagg ccagccatta cgctcgtcat caaaatcact cgcatcaacc
     8041 aaaccgttat tcattcgtga ttgcgcctga gcgaggcgaa atacgcgatc gctgttaaaa
     8101 ggacaattac aaacaggaat cgaatgcaac cggcgcagga acactgccag cgcatcaaca
     8161 atattttcac ctgaatcagg atattcttct aatacctgga atgctgtttt cccggggatc
     8221 gcagtggtga gtaaccatgc atcatcagga gtacggataa aatgcttgat ggtcggaaga
     8281 ggcataaatt ccgtcagcca gtttagtctg accatctcat ctgtaacatc attggcaacg
     8341 ctacctttgc catgtttcag aaacaactct ggcgcatcgg gcttcccata caatcgatag
     8401 attgtcgcac ctgattgccc gacattatcg cgagcccatt tatacccata taaatcagca
     8461 tccatgttgg aatttaatcg cggcctggag caagacgttt cccgttgaat atggctcata
     8521 acaccccttg tattactgtt tatgtaagca gacagtttta ttgttcatga tgatatattt
     8581 ttatcttgtg caatgtaaca tcagagattt tgagacacaa cgtggctttg ttgaataaat
     8641 cgaacttttg ctgagttgaa ggatcagtca tgaccaaaat cccttaacgt gagttttcgt
     8701 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     8761 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     8821 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     8881 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     8941 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     9001 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     9061 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     9121 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     9181 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     9241 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     9301 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     9361 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     9421 tggataaccg tgcggccgcc aatgctaaat tcgagtgaaa cacaggaaga tcagaaaatc
     9481 ctcatttcat ccatattaac aataatttca aatgtttatt tgcattattt gaaactaggc
     9541 aagacaagca acgaaacgtt tttgaaaatt ttgagtattt tcaataaatt tgtagaggac
     9601 tcagatattg aaaaaaagct acagcaatta atacttgata agaagagtat tgagaagggc
     9661 aacggttcat catctcatgg atctgcacat gaacaaacac cagagtcaaa cgacgttgaa
     9721 attgaggcta ctgcgccaat tgatgacaat acagacgatg ataacaaacc gaagttatct
     9781 gatgtagaaa aggattaaag atgctaagag atagtgatga tatttcataa ataatgtaat
     9841 tctatatatg ttaattacct tttttgcgag gcatatttat ggtgaaggat aagttttgac
     9901 catcaaagaa ggttaatgtg gctgtggttt cagggtccat aaagcccaca tggataacat
     9961 tacccctgaa ttcgcatcta gactgaactg gccgataatt gcagacgaac gccttgccaa
    10021 cagggagttc ttcagagaca tggaggctca aaacgaaatt attgacagcc tagacatcaa
    10081 tagtcataca acagaaagcg accacccaac tttggctgat aatagcgtat aaacaatgca
    10141 tactttgtac gttcaaaata caatgcagta gatatattta tgcatattac atataataca
    10201 tatcacatag gaagcaacag gcgcgttgga cttttaattt tcgaggaccg cgaatcctta
    10261 catcacaccc aatcccccac aagtgatccc ccacacacca tagcttcaaa atgtttctac
    10321 tcctttttta ctcttccaga ttttctcgga ctccgcgcat cgccgtacca cttcaaaaca
    10381 cccaagcaca gcatactaaa tttcccctct ttcttcctct agggtgtcgt taattacccg
    10441 tactaaaggt ttggaaaaga aaaaagacac cgcctcgttt ctttttcttc gtcgaaaaag
    10501 gcaataaaaa tttttatcac gtttcttttt cttgaaaatt tttttttttg atttttttct
    10561 ctttcgatga cctcccattg atatttaagt taataaacgg tcatcaattt ctcaagtttc
    10621 agtttcattt ttcttgttct attacaactt tttttacttc ttgctcatta gaaagaaagc
    10681 atagcaatct aatctaagtt ttaattacaa aagatctatg gataaagcgg aattaattcc
    10741 cgagcctcca aaaaagaaga gaaaggt
//
