LOCUS       sequence-270699-       11944 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Auxin signalling reporter (DR5rev) driving RFP (ER-targeted).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11944)
  AUTHORS   Marin E, Jouannet V, Herz A, Lokerse AS, Weijers D, Vaucheret H, 
            Nussaume L, Crespi MD, Maizel A
  TITLE     miR390, Arabidopsis TAS3 tasiRNAs, and their AUXIN RESPONSE FACTOR 
            targets define an autoregulatory network quantitatively regulating 
            lateral root growth.
  JOURNAL   Plant Cell. 2010 Apr;22(4):1104-17. doi: 10.1105/tpc.109.072553. 
            Epub 2010 Apr 2.
  PUBMED    20363771
REFERENCE   2  (bases 1 to 11944)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1105/tpc.109.072553"; journalName: "Plant Cell"; date: 
            "2010-04"; volume: "22"; issue: "4"; pages: "1104-17"
COMMENT     Sequence Label: DR5rev::erRFP
FEATURES             Location/Qualifiers
     source          1..11944
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    43..67
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        253..298
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     misc_feature    309..364
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     CDS             491..1162
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="ASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAKL
                     KVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGVV
                     TVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKMR
                     LKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHST
                     GA"
     CDS             491..1162
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /note="mammalian codon-optimized"
                     /translation="ASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAKL
                     KVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGVV
                     TVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKMR
                     LKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHST
                     GA"
     primer_bind     886..905
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     1080..1103
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     protein_bind    complement(1479..1503)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1513..3165
                     /codon_start=1
                     /gene="luc"
                     /product="firefly luciferase"
                     /label=luciferase
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     primer_bind     complement(1579..1597)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     misc_feature    3479..3503
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             4834..5463
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             5892..6965
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      7031..7225
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     misc_feature    7569..7709
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     7624..7643
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7724..7741)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7895..8483)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7975..7994)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(8729..9520)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_feature    10041..10065
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(10158..10410)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(10608..11633)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(11669..11848)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
ORIGIN
        1 ctcccatatg gtcgacctgc aggcggccgc actagtgata tcacaagttt gtacaaaaaa
       61 gcaggctccg cggccgcccc cttcacctcg acggtatcgc gcccagggag acaaaaggga
      121 gacaaaaggg agacaaaagg gagacaaaag ggagacaaaa gggagacaaa agggagacaa
      181 aagggagaca aaagggagac aaaagggggc aggcctcgat aagcttgata tcgaattaat
      241 tcctgcagcc ccgcaagacc cttcctctat ataaggaagt tcatttcatt tggagaggta
      301 tttttacaac aattaccaac aacaacaaac aacaaacaac attacaatta ctatttacaa
      361 ttacaattac aggggatcga tccaaggaga tataacaatg aagactaatc tttttctctt
      421 tctcatcttt tcacttctcc tatcattatc ctcggccgaa ttcggccggc ctggaggtgg
      481 aggtggagct gcctcctccg aggacgtcat caaggagttc atgcgcttca aggtgcgcat
      541 ggagggctcc gtgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta
      601 cgagggcacc cagaccgcca agctgaaggt gaccaagggc ggccccctgc ccttcgcctg
      661 ggacatcctg tcccctcagt tccagtacgg ctccaaggcc tacgtgaagc accccgccga
      721 catccccgac tacttgaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa
      781 cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg acggcgagtt
      841 catctacaag gtgaagctgc gcggcaccaa cttcccctcc gacggccccg taatgcagaa
      901 gaagaccatg ggctgggagg cctccaccga gcggatgtac cccgaggacg gcgccctgaa
      961 gggcgagatc aagatgaggc tgaagctgaa ggacggcggc cactacgacg ccgaggtcaa
     1021 gaccacctac atggccaaga agcccgtgca gctgcccggc gcctacaaga ccgacatcaa
     1081 gctggacatc acctcccaca acgaggacta caccatcgtg gaacagtacg agcgcgccga
     1141 gggccgccac tccaccggcg ccgatcctgc tggtgctgct gcggccgctg gggcccatga
     1201 tgagctttaa gatcctctag agtccgcaaa aatcaccagt ctctctctac aaatctatct
     1261 ctctctattt ttctccagaa taatgtgtga gtagttccca gataagggaa ttagggttct
     1321 tatagggttt cgctcatgtg ttgagcatat aagaaaccct tagtatgtat ttgtatttgt
     1381 aaaatacttc tatcaataaa atttctaatt cctaaaacca aaatccagtg acctgcaggc
     1441 atgctcctgt ggtctcgcta cgaagggtgg gcgcgccgac ccagctttct tgtacaaagt
     1501 ggtgataaaa aaatggaaga cgccaaaaac ataaagaaag gcccggcgcc attctatcct
     1561 ctagaggatg gaaccgctgg agagcaactg cataaggcta tgaagagata cgccctggtt
     1621 cctggaacaa ttgcttttac agatgcacat atcgaggtga acatcacgta cgcggaatac
     1681 ttcgaaatgt ccgttcggtt ggcagaagct atgaaacgat atgggctgaa tacaaatcac
     1741 agaatcgtcg tatgcagtga aaactctctt caattcttta tgccggtgtt gggcgcgtta
     1801 tttatcggag ttgcagttgc gcccgcgaac gacatttata atgaacgtga attgctcaac
     1861 agtatgaaca tttcgcagcc taccgtagtg tttgtttcca aaaaggggtt gcaaaaaatt
     1921 ttgaacgtgc aaaaaaaatt accaataatc cagaaaatta ttatcatgga ttctaaaacg
     1981 gattaccagg gatttcagtc gatgtacacg ttcgtcacat ctcatctacc tcccggtttt
     2041 aatgaatacg attttgtacc agagtccttt gatcgtgaca aaacaattgc actgataatg
     2101 aattcctctg gatctactgg gttacctaag ggtgtggccc ttccgcatag aactgcctgc
     2161 gtcagattct cgcatgccag agatcctatt tttggcaatc aaatcattcc ggatactgcg
     2221 attttaagtg ttgttccatt ccatcacggt tttggaatgt ttactacact cggatatttg
     2281 atatgtggat ttcgagtcgt cttaatgtat agatttgaag aagagctgtt tttacgatcc
     2341 cttcaggatt acaaaattca aagtgcgttg ctagtaccaa ccctattttc attcttcgcc
     2401 aaaagcactc tgattgacaa atacgattta tctaatttac acgaaattgc ttctgggggc
     2461 gcacctcttt cgaaagaagt cggggaagcg gttgcaaaac gcttccatct tccagggata
     2521 cgacaaggat atgggctcac tgagactaca tcagctattc tgattacacc cgagggggat
     2581 gataaaccgg gcgcggtcgg taaagttgtt ccattttttg aagcgaaggt tgtggatctg
     2641 gataccggga aaacgctggg cgttaatcag agaggcgaat tatgtgtcag aggacctatg
     2701 attatgtccg gttatgtaaa caatccggaa gcgaccaacg ccttgattga caaggatgga
     2761 tggctacatt ctggagacat agcttactgg gacgaagacg aacacttctt catagttgac
     2821 cgcttgaagt ctttaattaa atacaaagga tatcaggtgg cccccgctga attggaatcg
     2881 atattgttac aacaccccaa catcttcgac gcgggcgtgg caggtcttcc cgacgatgac
     2941 gccggtgaac ttcccgccgc cgttgttgtt ttggagcacg gaaagacgat gacggaaaaa
     3001 gagatcgtgg attacgtcgc cagtcaagta acaaccgcga aaaagttgcg cggaggagtt
     3061 gtgtttgtgg acgaagtacc gaaaggtctt accggaaaac tcgacgcaag aaaaatcaga
     3121 gagatcctca taaaggccaa gaagggcgga aagtccaaat tgtaaccgcg gccatgctag
     3181 agtccgcaaa aatcaccagt ctctctctac aaatctatct ctctctattt ttctccagaa
     3241 taatgtgtga gtagttccca gataagggaa ttagggttct tatagggttt cgctcatgtg
     3301 ttgagcatat aagaaaccct tagtatgtat ttgtatttgt aaaatacttc tatcaataaa
     3361 atttctaatt cctaaaacca aaatccagtg acctgcaggc atgcgacgtc gggcccaagc
     3421 ttagcttgag cttggatcag attgtcgttt cccgccttca gtttaaacta tcagtgtttg
     3481 acaggatata ttggcgggta aacctaagag aaaagagcgt ttattagaat aatcggatat
     3541 ttaaaagggc gtgaaaaggt ttatccgttc gtccatttgt atgtgcatgc caaccacagg
     3601 gttcccctcg ggatcaaagt actttaaagt actttaaagt actttaaagt actttgatcc
     3661 aacccctccg ctgctatagt gcagtcggct tctgacgttc agtgcagccg tcttctgaaa
     3721 acgacatgtc gcacaagtcc taagttacgc gacaggctgc cgccctgccc ttttcctggc
     3781 gttttcttgt cgcgtgtttt agtcgcataa agtagaatac ttgcgactag aaccggagac
     3841 attacgccat gaacaagagc gccgccgctg gcctgctggg ctatgcccgc gtcagcaccg
     3901 acgaccagga cttgaccaac caacgggccg aactgcacgc ggccggctgc accaagctgt
     3961 tttccgagaa gatcaccggc accaggcgcg accgcccgga gctggccagg atgcttgacc
     4021 acctacgccc tggcgacgtt gtgacagtga ccaggctaga ccgcctggcc cgcagcaccc
     4081 gcgacctact ggacattgcc gagcgcatcc aggaggccgg cgcgggcctg cgtagcctgg
     4141 cagagccgtg ggccgacacc accacgccgg ccggccgcat ggtgttgacc gtgttcgccg
     4201 gcattgccga gttcgagcgt tccctaatca tcgaccgcac ccggagcggg cgcgaggccg
     4261 ccaaggcccg aggcgtgaag tttggccccc gccctaccct caccccggca cagatcgcgc
     4321 acgcccgcga gctgatcgac caggaaggcc gcaccgtgaa agaggcggct gcactgcttg
     4381 gcgtgcatcg ctcgaccctg taccgcgcac ttgagcgcag cgaggaagtg acgcccaccg
     4441 aggccaggcg gcgcggtgcc ttccgtgagg acgcattgac cgaggccgac gccctggcgg
     4501 ccgccgagaa tgaacgccaa gaggaacaag catgaaaccg caccaggacg gccaggacga
     4561 accgtttttc attaccgaag agatcgaggc ggagatgatc gcggccgggt acgtgttcga
     4621 gccgcccgcg cacgtctcaa ccgtgcggct gcatgaaatc ctggccggtt tgtctgatgc
     4681 caagctggcg gcctggccgg ccagcttggc cgctgaagaa accgagcgcc gccgtctaaa
     4741 aaggtgatgt gtatttgagt aaaacagctt gcgtcatgcg gtcgctgcgt atatgatgcg
     4801 atgagtaaat aaacaaatac gcaaggggaa cgcatgaagg ttatcgctgt acttaaccag
     4861 aaaggcgggt caggcaagac gaccatcgca acccatctag cccgcgccct gcaactcgcc
     4921 ggggccgatg ttctgttagt cgattccgat ccccagggca gtgcccgcga ttgggcggcc
     4981 gtgcgggaag atcaaccgct aaccgttgtc ggcatcgacc gcccgacgat tgaccgcgac
     5041 gtgaaggcca tcggccggcg cgacttcgta gtgatcgacg gagcgcccca ggcggcggac
     5101 ttggctgtgt ccgcgatcaa ggcagccgac ttcgtgctga ttccggtgca gccaagccct
     5161 tacgacatat gggccaccgc cgacctggtg gagctggtta agcagcgcat tgaggtcacg
     5221 gatggaaggc tacaagcggc ctttgtcgtg tcgcgggcga tcaaaggcac gcgcatcggc
     5281 ggtgaggttg ccgaggcgct ggccgggtac gagctgccca ttcttgagtc ccgtatcacg
     5341 cagcgcgtga gctacccagg cactgccgcc gccggcacaa ccgttcttga atcagaaccc
     5401 gagggcgacg ctgcccgcga ggtccaggcg ctggccgctg aaattaaatc aaaactcatt
     5461 tgagttaatg aggtaaagag aaaatgagca aaagcacaaa cacgctaagt gccggccgtc
     5521 cgagcgcacg cagcagcaag gctgcaacgt tggccagcct ggcagacacg ccagccatga
     5581 agcgggtcaa ctttcagttg ccggcggagg atcacaccaa gctgaagatg tacgcggtac
     5641 gccaaggcaa gaccattacc gagctgctat ctgaatacat cgcgcagcta ccagagtaaa
     5701 tgagcaaatg aataaatgag tagatgaatt ttagcggcta aaggaggcgg catggaaaat
     5761 caagaacaac caggcaccga cgccgtggaa tgccccatgt gtggaggaac gggcggttgg
     5821 ccaggcgtaa gcggctgggt tgtctgccgg ccctgcaatg gcactggaac ccccaagccc
     5881 gaggaatcgg cgtgagcggt cgcaaaccat ccggcccggt acaaatcggc gcggcgctgg
     5941 gtgatgacct ggtggagaag ttgaaggccg cgcaggccgc ccagcggcaa cgcatcgagg
     6001 cagaagcacg ccccggtgaa tcgtggcaag cggccgctga tcgaatccgc aaagaatccc
     6061 ggcaaccgcc ggcagccggt gcgccgtcga ttaggaagcc gcccaagggc gacgagcaac
     6121 cagatttttt cgttccgatg ctctatgacg tgggcacccg cgatagtcgc agcatcatgg
     6181 acgtggccgt tttccgtctg tcgaagcgtg accgacgagc tggcgaggtg atccgctacg
     6241 agcttccaga cgggcacgta gaggtttccg cagggccggc cggcatggcc agtgtgtggg
     6301 attacgacct ggtactgatg gcggtttccc atctaaccga atccatgaac cgataccggg
     6361 aagggaaggg agacaagccc ggccgcgtgt tccgtccaca cgttgcggac gtactcaagt
     6421 tctgccggcg agccgatggc ggaaagcaga aagacgacct ggtagaaacc tgcattcggt
     6481 taaacaccac gcacgttgcc atgcagcgta cgaagaaggc caagaacggc cgcctggtga
     6541 cggtatccga gggtgaagcc ttgattagcc gctacaagat cgtaaagagc gaaaccgggc
     6601 ggccggagta catcgagatc gagctagctg attggatgta ccgcgagatc acagaaggca
     6661 agaacccgga cgtgctgacg gttcaccccg attacttttt gatcgatccc ggcatcggcc
     6721 gttttctcta ccgcctggca cgccgcgccg caggcaaggc agaagccaga tggttgttca
     6781 agacgatcta cgaacgcagt ggcagcgccg gagagttcaa gaagttctgt ttcaccgtgc
     6841 gcaagctgat cgggtcaaat gacctgccgg agtacgattt gaaggaggag gcggggcagg
     6901 ctggcccgat cctagtcatg cgctaccgca acctgatcga gggcgaagca tccgccggtt
     6961 cctaatgtac ggagcagatg ctagggcaaa ttgccctagc aggggaaaaa ggtcgaaaag
     7021 gtctctttcc tgtggatagc acgtacattg ggaacccaaa gccgtacatt gggaaccgga
     7081 acccgtacat tgggaaccca aagccgtaca ttgggaaccg gtcacacatg taagtgactg
     7141 atataaaaga gaaaaaaggc gatttttccg cctaaaactc tttaaaactt attaaaactc
     7201 ttaaaacccg cctggcctgt gcataactgt ctggccagcg cacagccgaa gagctgcaaa
     7261 aagcgcctac ccttcggtcg ctgcgctccc tacgccccgc cgcttcgcgt cggcctatcg
     7321 cggccgctgg ccgctcaaaa atggctggcc tacggccagg caatctacca gggcgcggac
     7381 aagccgcgcc gtcgccactc gaccgccggc gcccacatca aggcaccctg cctcgcgcgt
     7441 ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt
     7501 ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg
     7561 tgtcggggcg cagccatgac ccagtcacgt agcgatagcg gagtgtatac tggcttaact
     7621 atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca
     7681 gatgcgtaag gagaaaatac cgcatcaggc gctcttccgc ttcctcgctc actgactcgc
     7741 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     7801 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     7861 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     7921 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     7981 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     8041 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     8101 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     8161 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     8221 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     8281 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag
     8341 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     8401 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     8461 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     8521 agtggaacga aaactcacgt taagggattt tggtcatgca tgatatatct cccaatttgt
     8581 gtagggctta ttatgcacgc ttaaaaataa taaaagcaga cttgacctga tagtttggct
     8641 gtgagcaatt atgtgcttag tgcatctaac gcttgagtta agccgcgccg cgaagcggcg
     8701 tcggcttgaa cgaatttcta gctagacatt atttgccgac taccttggtg atctcgcctt
     8761 tcacgtagtg gacaaattct tccaactgat ctgcgcgcga ggccaagcga tcttcttctt
     8821 gtccaagata agcctgtcta gcttcaagta tgacgggctg atactgggcc ggcaggcgct
     8881 ccattgccca gtcggcagcg acatccttcg gcgcgatttt gccggttact gcgctgtacc
     8941 aaatgcggga caacgtaagc actacatttc gctcatcgcc agcccagtcg ggcggcgagt
     9001 tccatagcgt taaggtttca tttagcgcct caaatagatc ctgttcagga accggatcaa
     9061 agagttcctc cgccgctgga cctaccaagg caacgctatg ttctcttgct tttgtcagca
     9121 agatagccag atcaatgtcg atcgtggctg gctcgaagat acctgcaaga atgtcattgc
     9181 gctgccattc tccaaattgc agttcgcgct tagctggata acgccacgga atgatgtcgt
     9241 cgtgcacaac aatggtgact tctacagcgc ggagaatctc gctctctcca ggggaagccg
     9301 aagtttccaa aaggtcgttg atcaaagctc gccgcgttgt ttcatcaagc cttacggtca
     9361 ccgtaaccag caaatcaata tcactgtgtg gcttcaggcc gccatccact gcggagccgt
     9421 acaaatgtac ggccagcaac gtcggttcga gatggcgctc gatgacgcca actacctctg
     9481 atagttgagt cgatacttcg gcgatcaccg cttcccccat gatgtttaac tttgttttag
     9541 ggcgactgcc ctgctgcgta acatcgttgc tgctccataa catcaaacat cgacccacgg
     9601 cgtaacgcgc ttgctgcttg gatgcccgag gcatagactg taccccaaaa aaacagtcat
     9661 aacaagccat gaaaaccgcc actgcgccgt taccaccgct gcgttcggtc aaggttctgg
     9721 accagttgcg tgagcgcata cgctacttgc attacagctt acgaaccgaa caggcttatg
     9781 tccactgggt tcgtgcccga attgatcaca ggcagcaacg ctctgtcatc gttacaatca
     9841 acatgctacc ctccgcgaga tcatccgtgt ttcaaacccg gcagcttagt tgccgttctt
     9901 ccgaatagca tcggtaacat gagcaaagtc tgccgcctta caacggctct cccgctgacg
     9961 ccgtcccgga ctgatgggct gcctgtatcg agtggtgatt ttgtgccgag ctgccggtcg
    10021 gggagctgtt ggctggctgg tggcaggata tattgtggtg taaacaaatt gacgcttaga
    10081 caacttaata acacattgcg gacgttttta atgtactgaa ttaacgccga attgaattat
    10141 cagcttgcat gccggtcgat ctagtaacat agatgacacc gcgcgcgata atttatccta
    10201 gtttgcgcgc tatattttgt tttctatcgc gtattaaatg tataattgcg ggactctaat
    10261 cataaaaacc catctcataa ataacgtcat gcattacatg ttaattatta catgcttaac
    10321 gtaattcaac agaaattata tgataatcat cgcaagaccg gcaacaggat tcaatcttaa
    10381 gaaactttat tgccaaatgt ttgaacgatc tgcttgactc tagggaatta attcctgaat
    10441 cactgcgacc ggccctcccg cgacccagcc gagcgagctt agcgaactgt ggacgagaac
    10501 tgtgccacca agcgtaaggc cgttctctcg catttgcctt gctaggctcg cgcgagttgc
    10561 tggctgaggc gttctcgaaa tcagctcttg ttcggtcggc atctactcta ttcctttgcc
    10621 ctcggacgag tgctggggcg tcggtttcca ctatcggcga gtacttctac acagccatcg
    10681 gtccagacgg ccgcgcttct gcgggcgatt tgtgtacgcc cgacagtccc ggctccggat
    10741 cggacgattg cgtcgcatcg accctgcgcc caagctgcat catcgaaatt gccgtcaacc
    10801 aagctctgat agagttggtc aagaccaatg cggagcatat acgcccggag ccttggcgat
    10861 cctgcaagct ccggatgcct ccgctcgaag tagcgcgtct gctgctccat acaagccaac
    10921 cacggcctcc agaagaagat gttggcgacc tcgtattggg aatccccgaa catcgcctcg
    10981 ctccagtcaa tgaccgctgt tatgcggcca ttgtccgtca ggacattgtt ggagccgaaa
    11041 tccgcgtgca cgaggtgccg gacttcgggg cagtcctcgg cccaaagcat cagctcatcg
    11101 agagcctgcg cgacggacgc actgacggtg tcgtccatca cagtttgcca gtgatacaca
    11161 tggggatcag caatcgcgca tatgaaatca cgccatgtag tgtattgacc gattccttgc
    11221 ggtccgaatg ggccgaaccc gctcgtctgg ctaagatcgg ccgcagcgat agcatccata
    11281 gcctccgcga ccggctgaag aacagcgggc agttcggttt caggcaggtc ttgcaacgtg
    11341 acaccctgtg cacggcggga gatgcaatag gtcaggctct cgctgaactc cccaatgtca
    11401 agcacttccg gaatcgggag cgcggccgat gcaaagtgcc gataaacata acgatctttg
    11461 tagaaaccat cggcgcagct atttacccgc aggacatatc cacgccctcc tacatcgaag
    11521 ctgaaagcac gagattcttc gccctccgag agctgcatca ggtcggagac gctgtcgaac
    11581 ttttcgatca gaaacttctc gacagacgtc gcggtgagtt caggcttttt catagggggg
    11641 attcgagttg agagtgaata tgagactcta attggatacc gaggggaatt tatggaacgt
    11701 cagtggagca tttttgacaa gaaatatttg ctagctgata gtgaccttag gcgacttttg
    11761 aacgcgcaat aatggtttct gacgtatgtg cttagctcat taaactccag aaacccgcgg
    11821 ctcagtggct ccttcaacgt tgcggttctg tcagttccaa acgtaaaacg gcttgtcccg
    11881 cgtcatcggc gggggtcata acgtgactcc cttaattctc cgctcatgat cgataattcg
    11941 agct
//
