LOCUS       sequence-270705-        9748 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression vector for TAZ S89A mutant with N-terminal 
            FLAG and His tags.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9748)
  AUTHORS   Yang Z, Nakagawa K, Sarkar A, Maruyama J, Iwasa H, Bao Y, 
            Ishigami-Yuasa M, Ito S, Kagechika H, Hata S, Nishina H, Abe S, 
            Kitagawa M, Hata Y
  TITLE     Screening with a novel cell-based assay for TAZ activators 
            identifies a compound that enhances myogenesis in C2C12 cells and 
            facilitates muscle repair in the muscle injury model.
  JOURNAL   Mol Cell Biol. 2014 Feb 18.
  PUBMED    24550007
REFERENCE   2  (bases 1 to 9748)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 2014 Feb 18."
COMMENT     Sequence Label: pLenti-EF-FH-TAZ S89A-ires-blast
FEATURES             Location/Qualifiers
     source          1..9748
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        127..1289
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          358..1280
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1237..1257
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             1323..1346
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             1353..1370
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     misc_feature    2625..3197
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2791..2808)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3018..3037
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3243..3641
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     primer_bind     complement(3696..3716)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3701..4030
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3881..4016
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        4042..4089
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             4108..4482
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     LTR             4575..4808
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4880..5001
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4917..4936)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4971..4990
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5041..5176
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5196..5215)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5197..5215)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5225..5242)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5225..5241)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5234..5256)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5383..5838
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5470..5489)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5680..5701
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5864..5968
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5969..6037,6038..6829)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6187..6206)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7000..7588
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7489..7508
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7742..7759
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7876..7897
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7912..7917,7918..7935,7936..7942)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7950..7966
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7955..7977
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7974..7990
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7974..7990
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8009..8029
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8011..8029
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8057..8283
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8284..8464
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8511..8636
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9129..9362
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9547..9591
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga gagagagaca
       61 gagacagatc cattcgatta gtgaacggat ctcgacggta tcgattaatt aattgctagc
      121 tcgtgaggct ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag
      181 ttggggggag gggtcggcaa ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg
      241 gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata
      301 agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacaggta
      361 agtgccgtgt gtggttcccg cgggcctggc ctctttacgg gttatggccc ttgcgtgcct
      421 tgaattactt ccacctggct ccagtacgtg attcttgatc ccgagctgga gccaggggcg
      481 ggccttgcgc tttaggagcc ccttcgcctc gtgcttgagt tgaggcctgg cctgggcgct
      541 ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct ttcgataagt
      601 ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg caagatagtc
      661 ttgtaaatgc gggccaggat ctgcacactg gtatttcggt ttttggggcc gcgggcggcg
      721 acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga gcgcggccac
      781 cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct ggcctcgcgc
      841 cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca gttgcgtgag
      901 cggaaagatg gccgcttccc ggccctgctc cagggggctc aaaatggagg acgcggcgct
      961 cgggagagcg ggcgggtgag tcacccacac aaaggaaagg ggcctttccg tcctcagccg
     1021 tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat tagttctgga
     1081 gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg gagtttcccc
     1141 acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa ttctccttgg
     1201 aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca gtggttcaaa
     1261 gtttttttct tccatttcag gtgtcgtgaa gcttgttaac gtttaaacta gccggggcca
     1321 tggactacaa agacgatgac gacaaggaat tgcaccatca tcatcatcat gaattcaatc
     1381 cggcctcggc gccccctccg ctcccgccgc ctgggcagca agtgatccac gtcacgcagg
     1441 acctagacac agacctcgaa gccctcttca actctgtcat gaatccgaag cctagctcgt
     1501 ggcggaagaa gatcctgccg gagtctttct ttaaggagcc tgattcgggc tcgcactcgc
     1561 gccagtccag caccgactcg tcgggcggcc acccggggcc tcgactggct gggggtgccc
     1621 agcatgtccg ctcgcatgcg tcgcccgcgt ccctgcagct gggcaccggc gcgggtgctg
     1681 cgggtagccc cgcgcagcag cacgcgcacc tccgccagca gtcctacgac gtgaccgacg
     1741 agctgccact gcccccgggc tgggagatga ccttcacggc cactggccag aggtacttcc
     1801 tcaatcacat agaaaaaatc accacatggc aagaccctag gaaggcgatg aatcagcctc
     1861 tgaatcatat gaacctccac cctgccgtca gttccacacc agtgcctcag aggtccatgg
     1921 cagtatccca gccaaatctc gtgatgaatc accaacacca gcagcagatg gcccccagta
     1981 ccctgagcca gcagaaccac cccactcaga acccacccgc agggctcatg agtatgccca
     2041 atgcgctgac cactcagcag cagcagcagc agaaactgcg gcttcagaga atccagatgg
     2101 agagagaaag gattcgaatg cgccaagagg agctcatgag gcaggaagct gccctctgtc
     2161 gacagctccc catggaagct gagactcttg ccccagttca ggctgctgtc aacccaccca
     2221 cgatgacccc agacatgaga tccatcacta ataatagctc agatcctttc ctcaatggag
     2281 ggccatatca ttcgagggag cagagcactg acagtggcct ggggttaggg tgctacagtg
     2341 tccccacaac tccggaggac ttcctcagca atgtggatga gatggataca ggagaaaacg
     2401 caggacaaac acccatgaac atcaatcccc aacagacccg tttccctgat ttccttgact
     2461 gtcttccagg aacaaacgtt gacttaggaa ctttggaatc tgaagacctg atccccctct
     2521 tcaatgatgt agagtctgct ctgaacaaaa gtgagccctt tctaacctgg ctgtaactcg
     2581 acaattctgc atctagggcg gccaattccg cccctctccc cccccccctc tccccccccc
     2641 cccctaacgt tactggccga agccgcttgg aataaggccg gtgtgcgttt gtctatatgt
     2701 tattttccac catattgccg tcttttggca atgtgagggc ccggaaacct ggccctgtct
     2761 tcttgacgag cattcctagg ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga
     2821 atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag acaaacaacg tctgtagcga
     2881 ccctttgcag gcagcggaac cccccacctg gcgacaggtg cctctgcggc caaaagccac
     2941 gtgtataaga tacacctgca aaggcggcac aaccccagtg ccacgttgtg agttggatag
     3001 ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg aaggatgccc
     3061 agaaggtacc ccattgtatg ggatctgatc tggggcctcg gtgcacatgc tttacatgtg
     3121 tttagtcgag gttaaaaaaa cgtctaggcc ccccgaacca cggggacgtg gttttccttt
     3181 gaaaaacacg atgataagct tgccacaacc cgggatcctc tagcaaggtg aggaactaaa
     3241 ccatggccaa gcctttgtct caagaagaat ccaccctcat tgaaagagca acggctacaa
     3301 tcaacagcat ccccatctct gaagactaca gcgtcgccag cgcagctctc tctagcgacg
     3361 gccgcatctt cactggtgtc aatgtatatc attttactgg gggaccttgt gcagaactcg
     3421 tggtgctggg cactgctgct gctgcggcag ctggcaacct gacttgtatc gtcgcgatcg
     3481 gaaatgagaa caggggcatc ttgagcccct gcggacggtg ccgacaggtg cttctcgatc
     3541 tgcatcctgg gatcaaagcc atagtgaagg acagtgatgg acagccgacg gcagttggga
     3601 ttcgtgaatt gctgccctct ggttatgtgt gggagggcta agcacttcgt ggcggcccgc
     3661 gtaccggtta gtaatgagtt tggaattaat tctgtggaat gtgtgtcagt tagggtgtgg
     3721 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     3781 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     3841 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc
     3901 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     3961 ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     4021 cttttgcaaa aagctccccc tgttgacaat taatcatcgg catagtatat cggcatagta
     4081 taatacgaca aggtgaggaa ctaaaccatg gccaagttga ccagtgccgt tccggtgctc
     4141 accgcgcgcg acgtcgccgg agcggtcgag ttctggaccg accggctcgg gttctcccgg
     4201 gacttcgtgg aggacgactt cgccggtgtg gtccgggacg acgtgaccct gttcatcagc
     4261 gcggtccagg accaggtggt gccggacaac accctggcct gggtgtgggt gcgcggcctg
     4321 gacgagctgt acgccgagtg gtcggaggtc gtgtccacga acttccggga cgcctccggg
     4381 ccggccatga ccgagatcgg cgagcagccg tgggggcggg agttcgccct gcgcgacccg
     4441 gccggcaact gcgtgcactt cgtggccgag gagcaggact gacacgtgct acgagattta
     4501 aatggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa
     4561 agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc tgctttttgc
     4621 ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
     4681 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
     4741 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
     4801 ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg
     4861 aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa
     4921 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     4981 caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct aactccgccc
     5041 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     5101 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
     5161 ggcttttttg gaggcctagg gacgtaccca attcgcccta tagtgagtcg tattacgcgc
     5221 gctcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta
     5281 atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg
     5341 atcgcccttc ccaacagttg cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg
     5401 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
     5461 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
     5521 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
     5581 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
     5641 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
     5701 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt
     5761 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa
     5821 tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     5881 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     5941 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     6001 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     6061 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     6121 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     6181 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
     6241 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     6301 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     6361 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     6421 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     6481 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     6541 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     6601 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     6661 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     6721 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     6781 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     6841 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     6901 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     6961 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     7021 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     7081 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     7141 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     7201 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     7261 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     7321 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     7381 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     7441 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     7501 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     7561 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     7621 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     7681 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     7741 cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc
     7801 gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag
     7861 tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt
     7921 tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa
     7981 cagctatgac catgattacg ccaagcgcgc aattaaccct cactaaaggg aacaaaagct
     8041 ggagctgcaa gcttaatgta gtcttatgca atactcttgt agtcttgcaa catggtaacg
     8101 atgagttagc aacatgcctt acaaggagag aaaaagcacc gtgcatgccg attggtggaa
     8161 gtaaggtggt acgatcgtgc cttattagga aggcaacaga cgggtctgac atggattgga
     8221 cgaaccactg aattgccgca ttgcagagat attgtattta agtgcctagc tcgatacata
     8281 aacgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
     8341 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
     8401 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
     8461 agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac
     8521 gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta
     8581 cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta
     8641 ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga
     8701 aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta
     8761 atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat
     8821 cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca accctctatt
     8881 gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag
     8941 agcaaaacaa aagtaagacc accgcacagc aagcggccgc tgatcttcag acctggagga
     9001 ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa
     9061 ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca
     9121 gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca
     9181 gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag
     9241 aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc
     9301 atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc
     9361 ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct
     9421 agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac
     9481 agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag
     9541 caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg
     9601 tttaacataa caaattggct gtggtatata aaattattca taatgatagt aggaggcttg
     9661 gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat
     9721 tcaccattat cgtttcagac ccacctcc
//
