LOCUS       sequence-270708-         669 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Used to sense miRNA activity using fluorescence based tools. 
            hsa-miR-33b-5p target in 3' UTR of of mKate. miRNA activity can be 
            measured by the repression of mKate2 relative to EBFP2 fluorescent 
            proteins . Plasmid is inherently unstable; please see Depositor 
            Comments.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 669)
  AUTHORS   Gam JJ, Babb J, Weiss R
  TITLE     A mixed antagonistic/synergistic miRNA repression model enables 
            accurate predictions of multi-input miRNA sensor activity.
  JOURNAL   Nat Commun. 2018 Jun 22;9(1):2430. doi: 10.1038/s41467-018-04575-0.
  PUBMED    29934631
REFERENCE   2  (bases 1 to 669)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-06-22"; volume: "9(1):2430. doi"; pages: " 
            10.1038/s41467-018-04575-0"
COMMENT     Sequence Label: LSB-hsa-miR-33b-5p
FEATURES             Location/Qualifiers
     source          1..669
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(111..135)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(351..370)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    416..471
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(470..489)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
ORIGIN
        1 aatcaaggag gccgacaaag agacatacgt cgagcagcac gaggtggctg tggccagata
       61 ctgcgacctc cctagcaaac tggggcacaa acttaattga gaattctacc acccagcttt
      121 cttgtacaaa gtggtacgtc tcaagaccag aggtctctgc ttgggttcaa gcaatgcaac
      181 agcaatgcac gcaatgcaac agcaatgcac gcaatgcaac agcaatgcac gcaatgcaac
      241 agcaatgcac caaccagaag agacctgtgc ttcactcctc aggtgcaggc tgcctatcag
      301 aaggtggtgg ctggtgtggc caatgccctg gctcacaaat accactgaga tctttttccc
      361 tctgccaaaa attatgggga catcatgaag ccccttgagc atctgacttc tggctaataa
      421 aggaaattta ttttcattgc aatagtgtgt tggaattttt tgtgtctctc actcggaagg
      481 acatatggga gggcaaatca tttaaaacat cagaatgagt atttggttta gagtttggca
      541 acatatgccc atatgctggc tgccatgaac aaaggttggc tataaagagg tcatcagtat
      601 atgaaacagc cccctgctgt ccattcctta ttccatagaa aagccttgac ttgaggttag
      661 atttttttt
//
