LOCUS       sequence-270713-        9959 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  dox-dependent expression of mouse Eomes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9959)
  AUTHORS   Benchetrit H, Herman S, van Wietmarschen N, Wu T, Makedonski K, Maoz
            N, Yom Tov N, Stave D, Lasry R, Zayat V, Xiao A, Lansdorp PM, Sebban
            S, Buganim Y
  TITLE     Extensive Nuclear Reprogramming Underlies Lineage Conversion into 
            Functional Trophoblast Stem-like Cells.
  JOURNAL   Cell Stem Cell. 2015 Nov 5;17(5):543-56. doi: 
            10.1016/j.stem.2015.08.006. Epub 2015 Sep 24.
  PUBMED    26412562
REFERENCE   2  (bases 1 to 9959)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2015.08.006"; journalName: "Cell Stem Cell"; date: 
            "2015-11-5- 5"; volume: "17"; issue: "5"; pages: "543-56"
COMMENT     Sequence Label: FUW-TetO-Eomes
FEATURES             Location/Qualifiers
     source          1..9959
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    join(163..181,182..204,205..223,224..246,247..265,266..288,
                     289..307,308..330,331..349,350..372,373..391,392..414,
                     415..433)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        466..504
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     501..525
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     misc_feature    2200..2788
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2253..2273)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2671..2682)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2791..2807)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2792..2808)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             3313..3493
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(3519..3536)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3525..3749
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3795..4223
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3882..3901)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4092..4113
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4232..4252)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4237..4566
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4417..4552
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4479..4498
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        4614..4661
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             4680..5054
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    5184..5305
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5221..5240)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5275..5294
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5354..5370)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5354..5370)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5367..5389)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5378..5394
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5402..5408,5409..5426,5427..5432))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5447..5468
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5585..5602)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5756..6344)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5836..5855)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6515..7306,7307..7375))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7138..7157
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7376..7480)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7555..7574)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        7746..8125
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8127..8325
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             8343..8523
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8570..8695
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9188..9421
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9606..9650
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    join(9948..9959,1..106)
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat
       61 acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag
      121 ggacagcaga gatccagttt ggttagatct cgagtttacc actccctatc agtgatagag
      181 aaaagtgaaa gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt
      241 taccactccc tatcagtgat agagaaaagt gaaagtcgag tttaccactc cctatcagtg
      301 atagagaaaa gtgaaagtcg agtttaccac tccctatcag tgatagagaa aagtgaaagt
      361 cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta
      421 tcagtgatag agaaaagtga aagtcgagct cggtacccgg gtcgaggtag gcgtgtacgg
      481 tgggaggcct atataagcag agctcgttta gtgaaccgtc agatcgcctg gagacgccat
      541 ccacgctgtt ttgacctcca tagaagacac cgggaccgat ccagcctccg cggccccgaa
      601 ttcgccctta tgcagttggg agagcagctc ctggtgagct cggtgaacct gcccggcgcg
      661 cacttctact cgctggagag tgctcgcgga ggaggaggag gaggaggcgg aggaggaggg
      721 ggaggcggtc ctggcgccta tccctacggc cagggttctc cgctctacgg gccatacgcc
      781 ggaacctcag cggccgggtc ttgtggagga ttggggggcc taggggtgcc tggctccggc
      841 ttccgcgccc acgtctacct gtgcaaccgg cccctatggc tcaaattcca ccggcaccaa
      901 actgagatga tcatcaccaa acagggcagg cgcatgtttc ctttcttgag cttcaacata
      961 aacggactca accccaccgc ccactacaat gttttcgtgg aagtggttct ggccgaccct
     1021 aaccactggc gcttccaggg gggcaagtgg gtgacctgcg gcaaagcgga caataacatg
     1081 cagggcaata agatgtacgt tcacccagaa tctcctaaca ctggctccca ctggatgagg
     1141 caggagattt cctttgggaa gttaaaactc accaataaca aaggtgcaaa caacaacaac
     1201 acacagatga tagtgttgca gtctctgcac aaataccaac cgaggctgca catcgtggaa
     1261 gtgacagagg acggtgtgga ggacttgaat gaaccttcca agactcagac cttcaccttc
     1321 tcagagacac agttcatcgc tgtgacggcc taccaaaaca cggatatcac ccagctaaag
     1381 atcgaccata accccttcgc caaaggcttc cgggacaact acgattccat gtacacggct
     1441 tcagaaaatg acaggttaac tccatctccc acggattccc ctagatccca tcagattgtc
     1501 cctggaggtc ggtacggcgt tcaaaacttc ttcccggagc cctttgtcaa cactttgcct
     1561 caagcccgat attataacgg tgagagaacc gtgccacaga ccaacggcct cctctcaccc
     1621 caacagagcg aagaggtggc caaccctccc cagcggtggc ttgtcacgcc tgtccagcaa
     1681 cctgtgacca acaagctaga catcggttct tatgaatctg aatatacttc cagtaccttg
     1741 ctcccatatg gtattaagtc cttgcccctg cagacatccc atgccctggg gtattaccct
     1801 gacccgacct tccctgctat ggcagggtgg ggaggccgtg gcgcttatca gaggaagatg
     1861 gcagctggac taccatggac atccagaatg agcccacctg tcttcccaga agatcagctt
     1921 gccaaggaaa aagttaaaga agagattagt tcctcctgga tagagactcc cccctccatc
     1981 aagtctctag actccagcga ctccggggtg tacaacagcg cttgcaagag aaagcgcctg
     2041 tctcccagca cccccagcaa tggaaactcg ccccccataa agtgtgagga cattaacact
     2101 gaagagtaca gtaaagacac ctccaaaggc atgggggctt attatgcttt ttacacaagt
     2161 ccctagagca agggcgaatt cgatatcaag cttatcgata atcaacctct ggattacaaa
     2221 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
     2281 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
     2341 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
     2401 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc
     2461 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc
     2521 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
     2581 gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt
     2641 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc
     2701 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt
     2761 cggatctccc tttgggccgc ctccccgcat cgataccgtc gacctcgaga cctagaaaaa
     2821 catggagcaa tcacaagtag caatacagca gctaccaatg ctgattgtgc ctggctagaa
     2881 gcacaagagg aggaggaggt gggttttcca gtcacacctc aggtaccttt aagaccaatg
     2941 acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg
     3001 ctaattcact cccaacgaag acaagatatc cttgatctgt ggatctacca cacacaaggc
     3061 tacttccctg attggcagaa ctacacacca gggccaggga tcagatatcc actgaccttt
     3121 ggatggtgct acaagctagt accagttgag caagagaagg tagaagaagc caatgaagga
     3181 gagaacaccc gcttgttaca ccctgtgagc ctgcatggga tggatgaccc ggagagagaa
     3241 gtattagagt ggaggtttga cagccgccta gcatttcatc acatggcccg agagctgcat
     3301 ccggactgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     3361 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     3421 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     3481 aaaatctcta gcagggcccg tttaaacccg ctgatcagcc tcgactgtgc cttctagttg
     3541 ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc
     3601 cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc
     3661 tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag
     3721 gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc
     3781 tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
     3841 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
     3901 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
     3961 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg
     4021 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
     4081 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta
     4141 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat
     4201 ttaacaaaaa tttaacgcga attaattctg tggaatgtgt gtcagttagg gtgtggaaag
     4261 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     4321 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     4381 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     4441 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     4501 gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
     4561 tgcaaaaagc tcccgggagc ttgtatatcc attttcggat ctgatcagca cgtgttgaca
     4621 attaatcatc ggcatagtat atcggcatag tataatacga caaggtgagg aactaaacca
     4681 tggccaagtt gaccagtgcc gttccggtgc tcaccgcgcg cgacgtcgcc ggagcggtcg
     4741 agttctggac cgaccggctc gggttctccc gggacttcgt ggaggacgac ttcgccggtg
     4801 tggtccggga cgacgtgacc ctgttcatca gcgcggtcca ggaccaggtg gtgccggaca
     4861 acaccctggc ctgggtgtgg gtgcgcggcc tggacgagct gtacgccgag tggtcggagg
     4921 tcgtgtccac gaacttccgg gacgcctccg ggccggccat gaccgagatc ggcgagcagc
     4981 cgtgggggcg ggagttcgcc ctgcgcgacc cggccggcaa ctgcgtgcac ttcgtggccg
     5041 aggagcagga ctgacacgtg ctacgagatt tcgattccac cgccgccttc tatgaaaggt
     5101 tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca
     5161 tgctggagtt cttcgcccac cccaacttgt ttattgcagc ttataatggt tacaaataaa
     5221 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     5281 tgtccaaact catcaatgta tcttatcatg tctgtatacc gtcgacctct agctagagct
     5341 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
     5401 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
     5461 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     5521 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
     5581 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     5641 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     5701 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     5761 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     5821 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     5881 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     5941 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     6001 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     6061 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     6121 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     6181 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     6241 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     6301 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     6361 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     6421 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     6481 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     6541 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     6601 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     6661 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     6721 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     6781 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     6841 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     6901 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     6961 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     7021 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     7081 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     7141 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     7201 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     7261 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     7321 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     7381 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     7441 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     7501 cacctgacgt cgacggatcg ggagatctcc cgatccccta tggtgcactc tcagtacaat
     7561 ctgctctgat gccgcatagt taagccagta tctgctccct gcttgtgtgt tggaggtcgc
     7621 tgagtagtgc gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg acaattgcat
     7681 gaagaatctg cttagggtta ggcgttttgc gctgcttcgc gatgtacggg ccagatatac
     7741 gcgttgacat tgattattga ctagttatta atagtaatca attacggggt cattagttca
     7801 tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc
     7861 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat
     7921 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
     7981 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc
     8041 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta
     8101 cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg
     8161 atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt
     8221 gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac
     8281 gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagcgcgt tttgcctgta
     8341 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     8401 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
     8461 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     8521 gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg
     8581 caggactcgg cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac
     8641 gccaaaaatt ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat
     8701 taagcggggg agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa
     8761 aaaatataaa ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa
     8821 tcctggcctg ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc
     8881 ccttcagaca ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg
     8941 tgtgcatcaa aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga
     9001 gcaaaacaaa agtaagacca ccgcacagca agcggccgct gatcttcaga cctggaggag
     9061 gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac
     9121 cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag
     9181 tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag
     9241 cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga
     9301 acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca
     9361 tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc
     9421 tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta
     9481 gttggagtaa taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca
     9541 gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc
     9601 aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt
     9661 ttaacataac aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg
     9721 taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt
     9781 caccattatc gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa
     9841 tagaagaaga aggtggagag agagacagag acagatccat tcgattagtg aacggatcgg
     9901 cactgcgtgc gccaattctg cagacaaatg gcagtattca tccacaattt taaaagaaa
//
