LOCUS       sequence-270742-        9087 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral inducible expression (TetOn) of mouse PTTG1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9087)
  TITLE     de Carcer unpublished plasmids
REFERENCE   2  (bases 1 to 9087)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pLENTI_TetOn_Hu_PTTG1(WT)
FEATURES             Location/Qualifiers
     source          1..9087
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        65..379
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    73..91
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    109..127
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    144..162
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    180..198
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    216..234
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    251..269
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    287..305
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     352..376
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    457..481
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1115..1139)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    1170..1758
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1223..1243)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1641..1652)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        1814..2313
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(1849..1872)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     2246..2265
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             2331..3356
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             2331..3356
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     LTR             3730..3963
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4035..4156
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4072..4091)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4126..4145
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4196..4331
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4351..4370)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4352..4370)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4380..4397)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4380..4396)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4389..4411)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4538..4993
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4625..4644)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4835..4856
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5019..5123
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5124..5192,5193..5984)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5342..5361)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6155..6743
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6644..6663
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6897..6914
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7031..7052
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7067..7072,7073..7090,7091..7097)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7105..7121
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7110..7132
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7129..7145
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7129..7145
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7164..7184
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7166..7184
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7212..7438
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7439..7619
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7666..7791
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8284..8517
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8702..8746
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    join(9013..9087,1..43)
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttatcgatc acgagactag
       61 cctcgagttt actccctatc agtgatagag aacgtatgtc gagtttactc cctatcagtg
      121 atagagaacg atgtcgagtt tactccctat cagtgataga gaacgtatgt cgagtttact
      181 ccctatcagt gatagagaac gtatgtcgag tttactccct atcagtgata gagaacgtat
      241 gtcgagttta tccctatcag tgatagagaa cgtatgtcga gtttactccc tatcagtgat
      301 agagaacgta tgtcgaggta ggcgtgtacg gtgggaggcc tatataagca gagctcgttt
      361 agtgaaccgt cagatcgcct ggagaattcg agctcggtac ccggggatcc accggttcgt
      421 cgactagtcc agtgtggtgg aattctgcag atatcaacaa gtttgtacaa aaaagcaggc
      481 tccgcggccg cccccttcac catggctact cttatctttg ttgataagga taatgaagaa
      541 cccggcagcc gtttggcttc taaggatggg ttgaagctgg gctctggtgt caaggcctta
      601 gatgggaaat tgcaggtttc aacgcctcga gtcggcaaag tgttcaatgc tccagccttg
      661 cctaaagcca gcagaaaggc tttggggaca gtcaacagag ttgccgaaaa gcctatgaag
      721 actggtaaac ccctccaacc aaaacagccg accttgactg ggaaaaagat caccgagaag
      781 tctactaaga cacaaagttc tgttcctgct cctgatgatg cctacccaga aatagaaaag
      841 ttcttccctt tcaatcctct agattttgag agttttgacc tgcctgagga gcaccagatc
      901 tcacttctcc ccttgaatgg cgtgcctctc atgaccctga atgaagagag agggctggag
      961 aagctgctgc atctgggccc ccctagccct ctgaagacac cctttctatc atgggaatct
     1021 gatccgctgt actctcctcc cagtgccctc tccactctgg atgttgaatt gccacctgtt
     1081 tgttacgatg cagatattaa gggtgggcgc gccgacccag ctttcttgta caaagtggtt
     1141 gatatccagc acagtggcgg ccgctcgaca atcaacctct ggattacaaa atttgtgaaa
     1201 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa
     1261 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat
     1321 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt
     1381 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc
     1441 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc
     1501 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg
     1561 ggaagctgac gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga
     1621 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc
     1681 tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc
     1741 tttgggccgc ctccccgcct ggaattctgc agatatccgg actagtgatc tctcgaggtt
     1801 aacgaattct accgggtagg ggaggcgctt ttcccaaggc agtctggagc atgcgcttta
     1861 gcagccccgc tgggcacttg gcgctacaca agtggcctct ggcctcgcac acattccaca
     1921 tccaccggta ggcgccaacc ggctccgttc tttggtggcc ccttcgcgcc accttctact
     1981 cctcccctag tcaggaagtt cccccccgcc ccgcagctcg cgtcgtgcag gacgtgacaa
     2041 atggaagtag cacgtctcac tagtctcgtg cagatggaca gcaccgctga gcaatggaag
     2101 cgggtaggcc tttggggcag cggccaatag cagctttgct ccttcgcttt ctgggctcag
     2161 aggctgggaa ggggtgggtc cgggggcggg ctcaggggcg ggctcagggg cggggcgggc
     2221 gcccgaaggt cctccggagg cccggcattc tgcacgcttc aaaagcgcac gtctgccgcg
     2281 ctgttctcct cttcctcatc tccgggcctt tcgacctgca tcccgccacc atgaaaaagc
     2341 ctgaactcac cgcgacgtct gtcgagaagt ttctgatcga aaagttcgac agcgtctccg
     2401 acctgatgca gctctcggag ggcgaagaat ctcgtgcttt cagcttcgat gtaggagggc
     2461 gtggatatgt cctgcgggta aatagctgcg ccgatggttt ctacaaagat cgttatgttt
     2521 atcggcactt tgcatcggcc gcgctcccga ttccggaagt gcttgacatt ggggaattca
     2581 gcgagagcct gacctattgc atctcccgcc gtgcacaggg tgtcacgttg caagacctgc
     2641 ctgaaaccga actgcccgct gttctgcagc cggtcgcgga ggccatggat gcgatcgctg
     2701 cggccgatct tagccagacg agcgggttcg gcccattcgg accgcaagga atcggtcaat
     2761 acactacatg gcgtgatttc atatgcgcga ttgctgatcc ccatgtgtat cactggcaaa
     2821 ctgtgatgga cgacaccgtc agtgcgtccg tcgcgcaggc tctcgatgag ctgatgcttt
     2881 gggccgagga ctgccccgaa gtccggcacc tcgtgcacgc ggatttcggc tccaacaatg
     2941 tcctgacgga caatggccgc ataacagcgg tcattgactg gagcgaggcg atgttcgggg
     3001 attcccaata cgaggtcgcc aacatcttct tctggaggcc gtggttggct tgtatggagc
     3061 agcagacgcg ctacttcgag cggaggcatc cggagcttgc aggatcgccg cggctccggg
     3121 cgtatatgct ccgcattggt cttgaccaac tctatcagag cttggttgac ggcaatttcg
     3181 atgatgcagc ttgggcgcag ggtcgatgcg acgcaatcgt ccgatccgga gccgggactg
     3241 tcgggcgtac acaaatcgcc cgcagaagcg cggccgtctg gaccgatggc tgtgtagaag
     3301 tactcgccga tagtggaaac cgacgcccca gcactcgtcc gagggcaaag gaatagagta
     3361 gatgccgacc gaacaagagc tgatttcgag aacgcctcag ccagcaactc gcgcgagcct
     3421 agcaaggcaa atgcgagaga acggccttac gcttggtggc acagttctcg tccacagttc
     3481 gctaagctcg ctcggctggg tcgcgggagg gccggtcgca gtgattcagg cccttctgga
     3541 ttgtgttggt ccccagggca cgattgtcat gcccacgcac tcgggtgatc tgactgatcc
     3601 cgcagattgg agatcgccgc ccgtgcctgc cgattgggtg cagatccgtc gagggcccgg
     3661 tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa
     3721 aggggggact ggaagggcta gctcactccc aacgaagaca agatctgctt tttgcttgta
     3781 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     3841 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
     3901 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     3961 gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat
     4021 cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     4081 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     4141 tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc cgcccatccc
     4201 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     4261 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
     4321 ttttggaggc ctagggacgt acccaattcg ccctatagtg agtcgtatta cgcgcgctca
     4381 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc
     4441 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc
     4501 ccttcccaac agttgcgcag cctgaatggc gaatgggacg cgccctgtag cggcgcatta
     4561 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     4621 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     4681 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     4741 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     4801 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     4861 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     4921 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta
     4981 acgcttacaa tttaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     5041 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     5101 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     5161 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     5221 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     5281 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     5341 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
     5401 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     5461 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     5521 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     5581 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     5641 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
     5701 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     5761 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     5821 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     5881 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     5941 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     6001 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     6061 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     6121 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     6181 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     6241 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     6301 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     6361 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     6421 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     6481 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     6541 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     6601 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     6661 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     6721 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     6781 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     6841 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     6901 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
     6961 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
     7021 gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc
     7081 ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct
     7141 atgaccatga ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc
     7201 tgcaagctta atgtagtctt atgcaatact cttgtagtct tgcaacatgg taacgatgag
     7261 ttagcaacat gccttacaag gagagaaaaa gcaccgtgca tgccgattgg tggaagtaag
     7321 gtggtacgat cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac
     7381 cactgaattg ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg
     7441 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     7501 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     7561 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     7621 tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg
     7681 actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca
     7741 aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag
     7801 cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa
     7861 tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct
     7921 ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt
     7981 cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg
     8041 catcaaagga tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa
     8101 aacaaaagta agaccaccgc acagcaagcg gccgctgatc ttcagacctg gaggaggaga
     8161 tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt
     8221 aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg
     8281 aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc
     8341 aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa
     8401 tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa
     8461 gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg
     8521 gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg
     8581 gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga
     8641 aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga
     8701 aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa
     8761 cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg
     8821 tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc
     8881 attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga
     8941 agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg gatctcgacg
     9001 gtatcggtta acttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat
     9061 agtagacata atagcaacag acataca
//
