LOCUS       sequence-270744-        3177 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mouse PTTG1 cDNA in Gateway pENTR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3177)
  TITLE     de Carcer unpublished plasmids
REFERENCE   2  (bases 1 to 3177)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pENTR_Hu_PTTG1(WT)
FEATURES             Location/Qualifiers
     source          1..3177
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     173..192
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     426..443
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(602..629)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(721..807)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     856..878
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     870..887
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     871..887
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    903..1002
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1636..1735)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1752..1771)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1753..1771)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1776..1792)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1776..1792)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1894..1913)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1905..2714
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1972..1991)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      join(2861..3177,1..272)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
       61 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
      121 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
      181 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
      241 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
      301 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
      361 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
      421 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
      481 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
      541 gcagtgagcg caacgcaatt aatacgcgta ccgctagcca ggaagagttt gtagaaacgc
      601 aaaaaggcca tccgtcagga tggccttctg cttagtttga tgcctggcag tttatggcgg
      661 gcgtcctgcc cgccaccctc cgggccgttg cttcacaacg ttcaaatccg ctcccggcgg
      721 atttgtccta ctcaggagag cgttcaccga caaacaacag ataaaacgaa aggcccagtc
      781 ttccgactga gcctttcgtt ttatttgatg cctggcagtt ccctactctc gcgttaacgc
      841 tagcatggat gttttcccag tcacgacgtt gtaaaacgac ggccagtctt aagctcgggc
      901 cccaaataat gattttattt tgactgatag tgacctgttc gttgcaacaa attgatgagc
      961 aatgcttttt tataatgcca actttgtaca aaaaagcagg ctccgcggcc gcccccttca
     1021 ccatggctac tcttatcttt gttgataagg ataatgaaga acccggcagc cgtttggctt
     1081 ctaaggatgg gttgaagctg ggctctggtg tcaaggcctt agatgggaaa ttgcaggttt
     1141 caacgcctcg agtcggcaaa gtgttcaatg ctccagcctt gcctaaagcc agcagaaagg
     1201 ctttggggac agtcaacaga gttgccgaaa agcctatgaa gactggtaaa cccctccaac
     1261 caaaacagcc gaccttgact gggaaaaaga tcaccgagaa gtctactaag acacaaagtt
     1321 ctgttcctgc tcctgatgat gcctacccag aaatagaaaa gttcttccct ttcaatcctc
     1381 tagattttga gagttttgac ctgcctgagg agcaccagat ctcacttctc cccttgaatg
     1441 gcgtgcctct catgaccctg aatgaagaga gagggctgga gaagctgctg catctgggcc
     1501 cccctagccc tctgaagaca ccctttctat catgggaatc tgatccgctg tactctcctc
     1561 ccagtgccct ctccactctg gatgttgaat tgccacctgt ttgttacgat gcagatatta
     1621 agggtgggcg cgccgaccca gctttcttgt acaaagttgg cattataaga aagcattgct
     1681 tatcaatttg ttgcaacgaa caggtcacta tcagtcaaaa taaaatcatt atttgccatc
     1741 cagctgatat cccctatagt gagtcgtatt acatggtcat agctgtttcc tggcagctct
     1801 ggcccgtgtc tcaaaatctc tgatgttaca ttgcacaaga taaaaatata tcatcatgaa
     1861 caataaaact gtctgcttac ataaacagta atacaagggg tgttatgagc catattcaac
     1921 gggaaacgtc gaggccgcga ttaaattcca acatggatgc tgatttatat gggtataaat
     1981 gggctcgcga taatgtcggg caatcaggtg cgacaatcta tcgcttgtat gggaagcccg
     2041 atgcgccaga gttgtttctg aaacatggca aaggtagcgt tgccaatgat gttacagatg
     2101 agatggtcag actaaactgg ctgacggaat ttatgcctct tccgaccatc aagcatttta
     2161 tccgtactcc tgatgatgca tggttactca ccactgcgat ccccggaaaa acagcattcc
     2221 aggtattaga agaatatcct gattcaggtg aaaatattgt tgatgcgctg gcagtgttcc
     2281 tgcgccggtt gcattcgatt cctgtttgta attgtccttt taacagcgat cgcgtatttc
     2341 gtctcgctca ggcgcaatca cgaatgaata acggtttggt tgatgcgagt gattttgatg
     2401 acgagcgtaa tggctggcct gttgaacaag tctggaaaga aatgcataaa cttttgccat
     2461 tctcaccgga ttcagtcgtc actcatggtg atttctcact tgataacctt atttttgacg
     2521 aggggaaatt aataggttgt attgatgttg gacgagtcgg aatcgcagac cgataccagg
     2581 atcttgccat cctatggaac tgcctcggtg agttttctcc ttcattacag aaacggcttt
     2641 ttcaaaaata tggtattgat aatcctgata tgaataaatt gcagtttcat ttgatgctcg
     2701 atgagttttt ctaatcagaa ttggttaatt ggttgtaaca ctggcagagc attacgctga
     2761 cttgacggga cggcgcaagc tcatgaccaa aatcccttaa cgtgagttac gcgtcgttcc
     2821 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     2881 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     2941 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     3001 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     3061 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     3121 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggct
//
