LOCUS       sequence-27081-e         698 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 698)
  AUTHORS   Oancea E, Teruel MN, Quest AF, Meyer T
  TITLE     Green fluorescent protein (GFP)-tagged cysteine-rich domains from 
            protein kinase C as fluorescent indicators for diacylglycerol 
            signaling in living cells.
  JOURNAL   J Cell Biol. 1998 Feb 9. 140(3):485-98.
  PUBMED    9456311
REFERENCE   2  (bases 1 to 698)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol. 1998 Feb 9. 140(3):485-98."
COMMENT     Sequence Label: GFP-C1-PKCgamma-C1A
FEATURES             Location/Qualifiers
     source          1..698
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    399..520
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(436..455)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     490..509
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(664..685)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 tctcggcatg gacgagctgt acaagtccgg ccggactcag atctcgagct caagcttcga
       61 attctgcagt cgacggtacc ttggggcggg ccacaagttc accgctcgtt tcttcaagca
      121 gccaaccttc tgcagtcact gtaccgactt catctggggc attggaaagc agggcctgca
      181 atgtcaagtc tgcagctttg tggttcaccg ccgatgccac gaatttgtga ccttcgagtg
      241 tccaggagct ggacggtacc gcgggcccgg gatccaccgg atctagataa ctgatcataa
      301 tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc
      361 tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata
      421 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
      481 attctagttg tggtttgtcc aaactcatca atgtatctta acgcgtaaat tgtaagcgtt
      541 aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag
      601 gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt
      661 gttccagttt ggaacaagag tccactatta aagaacat
//
